운동강도의 차이가 대장에서의 Heme oxygenase-1 발현에 미치는 영향: Oligonucleotide chip microarray analysis

최은주 ( Eun Ju Choi ),류호영 ( Ho Young Ryu ),차광석 ( Kwang Suk Cha ) 한국운동생리학회(구-한국운동과학회) 2012 운동과학 Vol.21 No.1

이 연구의 목적은 운동강도 차이에 의한 대장조직이 Heme oxygenase-1(HO-1)의 mRNA 발현에 어떠한 영향을 미치는지 규명하기 위해, Sprage-Dawley계 흰쥐를 대상으로 통제그룹(CON)과 저강도 운동그룹(LIE), 고강도 운동그룹(HIE)으로 구분하여 4주 동안 트레드밀운동 후 48시간 이후에 High through-put microarray analysis방법으로 다음과 같은 실험을 실시하였다. 본 실험은 Rat ABI oligo chip 26,857 개의 유전자 중 filtering을 통하여 12.079개 유전지를 선택하였고, 이 중 유의성 있는(p<.05) 유전자 12,072개를 선별하였으며, clustering분석 방법과 면역조직화학염색법에서도 HO-l이 운동강도에 따라 유의하게 발현하였다(p<.05). Microarray분석 후 RT-PCR로 확인한 결과 HO-I의 유전자 발현양상이 일치하는 결과를 얻었다. 이 실험의 결과로서 운동이 대장에서 HO-1 발현에 영향을 주는 것을 알 수 있었다. 운동이 대장에서의 mRNA 발현이 저강도 운동그룹(LIE)보다 고강도 운동그룹(HIE)에서 더 많이 발현되어지는 것으로 보아 고강도 운동에 의해 발생되는 산소라디칼을 HO-l의 유도를 통하여 적절히 제거함으로써 스스로 항상성을 유지하고 있는 것으로 생각되어진다. The purpose of this study is to identify how large intestine tissue effects to mRNA expression in Heme oxygenase-l (HO-1) due to differences in exercise intensity. To perform this experiment we divided Sprage-Dawley rats into 3 groups (control group (CON). low-intensity exercise group (LIE) and high-intensity exercise group (HIE)), and in 48 hours after 4 weeks treadmill exercise. the following experiment was performed with "High through-put micro array analysis methods" . 12,079 genes were chosen by filtering of the Rat ASI oligo chip 26.857 genes in this experiment Among 12.079 genes, we selected significant genes (p<.05) that were also expressed in the ways of clustering analysis and immunohistochemistry. With results of RT-PCR confirming and microarray analyzing, we derived that gene expression profiles of HO-l had been consistent. As a result of this experiment, we certainly identified that exercise effects to HQ-l expression in large intestine. mRNA expression was more expressed in high-intensity exercise group CHIE) than low-intensity exercise group (LIE), it seems that homeostasis maintains itself by properly removing oxygen radical. caused by high intensity exercise. through reduction of HO-l.

Arch교의 橫桁連結部 局部應力과 疲勞擧動에 관한 硏究

최광순,조호근,손영호,최광규 동아대학교 건설기술연구소 1997 硏究報告 Vol.21 No.2

In this paper, the relation between the local stress and the fatigue behavior at gusset plate connected with main girder and floor beam in arch bridges was obtained. The conclusion was as follows : (1) In-plane bending moment and out-of-plane bending moment are higher than other internal stress resultants. (2) When a vehicle crosses the bridges, the change of out-of-plane bending moment corresponds with the change of relative difference in displacement in the longitudinal direction between the tie-girder and stringer. (3) The change of in-plane bending moment corresponds with the change of the displacement in the transverse direction of arch-rib. (4) Fatigue crack can be initiated by in-plane bending stress that Is produced by out-of-plane bending moment at the toe of gusset plate. (5) Fatigue crack can be initiated by tension stress that is produced by in-plane bending moment at the toe of gusset plate.

Arch교의 橫桁連結部 局部應力과 疲勞擧動에 관한 硏究

최광순,조호근,손영호,최광규 東亞大學校 建設技術硏究所 1997 硏究論文集 Vol.21 No.2

In this paper, the relation between the local stress and the fatigue behavior at gusset plate connected with main girder and floor beam in arch bridges was obtained. The conclusion was as follows : (1) In-plane bending moment and out-of-plane bending moment are higher than other internal stress resultants. (2) When a vehicle crosses the bridges, the change of out-of-plane bending moment corresponds with the change of relative difference in displacement in the longitudinal direction between the tie-girder and stringer. (3) The change of in-plane bending moment corresponds with the change of the displacement in the transverse direction of arch-rib. (4) Fatigue crack can be initiated by in-plane bending stress that is produced by out-of-plane bending moment at the toe of gusset plate. (5) Fatigue crack can be initiated by tension stress that is produced by in-plane bending moment at the toe of gusset plate.

Azithromycin으로 치료한 임신 중 쓰쓰가무시병 1예

김광석,최진욱,서호종,김기훈,박성호,서광섭,고성만,김순혜,김호정 대한감염학회 2001 감염 Vol.33 No.5

We report a case of tsutsugamushi disease in a 26 year-old pregnant woman who was treated with azithromycin. Her gestation period was 27 weeks and she admitted with fever, rash, and eschar on the right shoulder. Currently recommended medications for the treatment of scrub typhus are doxycycline or chloramphenicol. But, these drugs are class D drugs according to the U.S. Food and Drug Administration (FDA) Fetal Risk Summary, so they couldn't be used to treat pregnant women. Recently, a few case reports suggested that azithromycin, a relatively new macrolide antibiotic, was effective and safe for the treatment of scrub typhus in pregnant women. And, there is no evidence that azithromycin causes harmful effects to the developing fetus or to children. On the basis of current in vivo test that confirms the effectiveness of azithromycin, it may be the drug of choice for the treatment of scrub typhus in pregnant women and children. (Korean J Infect Dis 33:380-382, 2001)

국산 Lidocaine 알칼리화를 위한 Bicarbonate 혼합시 침전유발 최소량에 대한 고찰

이선호,김현수,곽인숙,안원식,최관호,김광민,손민제 대한마취과학회 2000 Korean Journal of Anesthesiology Vol.39 No.5

Background : When local anesthetics for regional anesthesia is used, usually small amounts of bicar-bonate are added for rapid onset. This addition gives the mixed solution a more alkaline pH. The following result is an increased unionized form of the local anesthetic and rapid penetration of the drug into tiissue. Unfortunately, no data about adequate mixing volumes of domestic lidocaine and bicarbonate is available. Methods : We examined six mixing pairs of two kinds of 2% lidocaines and three kinds of 8.4% bicarbonates for minimum volumes of bicarbonate to cause a precipitation of 2% 20 ml lidocaine. Results : The mean volumes of bicarbonate to cause precipitation were 1.54 ml for Kwang-Myung lidocaine with Kwang-Myung bicarbonate, 2.90 ml for Kwang-Myung lidocaine with Dae-Won bicarbonate, 2.73 ml for Kwang-Myung lidocaine with Je-Il bicarbonate, 0.97 ml for Je-Il lidocaine with Kwang-Myung bicarbonate, 1.26ml for Je-Il lidocaine with Dae-Won bicarbonate and 1.39 ml for Je-Il lidocaine with Je-Il bicarbonate. Conclusions : We conclude that the Kwang-Myung lidocaine and the Je-Il lidocaine could cause precipitation when mixing with a smaller bicarbonate volume than foreign textbook recommended. (Korean J Anesthesiol 2000; 39: 726-729)

성인에서 발생한 설사 동반성 용혈성 요독 증후군 3례

김진호,장광열,김구,배성진,김성은,김기현,최필선 동아대학교의과대학 1997 동아의대학술지 Vol.9 No.2

고온에 노출된 콘크리트의 손상 깊이에 따른 색상변화와 잔존압축강도

최광호 남서울대학교 2005 남서울대학교 논문집 Vol.11 No.2

The determination of the depth of deteriorated concrete is one of the main problems in the structural assessment of concrete structures that have been subjected to a fire. This information is particularly important in order to optimize the future operations of repair/strengthening, or in decision-making concerning a possible demolition. The purpose of this study is to propose evaluation technique of damaged depth of concrete exposed at high temperature and to investigate the relation between color change and residual compressive strength. In order to evaluate damaged depth of core picked at member under fire, the 24 specimens have been made with variables of concrete strength(20MPa, 40MPa, 60MPa) and heating exposure condition in 600℃ and 800℃ for 2 hours. Color change analysis at split specimen after heating have been measured and split tensile stress test was performed to find the residual compressive strength against the depth of specimen. The results show that the residual strength of specimens are coincident with the full development of the pink/red color and the method may be used to define the distance from a heated surface where strength degradation has occurred.

상악 후방의 부분 무치악부에 Sintered porous surface implant의 적용에 관한 문헌고찰 및 증례보고

최희준,이광호,이영석,손경준 조선대학교 구강생물학연구소 2002 Oral Biology Research (Oral Biol Res) Vol.26 No.2

Implant therapy based on the principle of osseointegration has seen a remarkable expansion of its application in dentistry in recent years. This article is a consideration of an endosseous dental implant device(Endopcre, Innova) that uses a sintered porous surface geometry to achieve implant fixation by bone ingrowth. Its implant is documented excellent survival rate(97.2% after a mean functional time of 34.6 months) in Partially edentulous maxillae and insignificant crestal bone loss from baseline. The combination of an innovative, minimally invasive, indirect sinus elevation procedure with a porous-surfaced dental implant system may allow the routine treatment of posterior maxillary site in patients who would otherwise require much more sophisticated and expensive reconstructive surgery to receive longer dental implants .

고속 가공기의 스핀들 진동에 영향을 주는 요소 분석

최영호,윤두표,김광영,최병오 한국공작기계학회 2001 한국공작기계학회 춘계학술대회논문집 Vol.2001 No.-

In this paper, We have studied on the critical vibration limits of spindle unit for the high speed ball pen tip processing machine. The vibration of bearing can be measured by FFT, and the influence of vibration amplitude due to the Unbalance, bearing deflect, bite and timing belts tension are analyzed. So, the critical vibration limits of spindle is determined by the X, Z directional vibration of spindle Unit.

철근콘크리트 기둥과 철골보로 구성된 복합구조 접합부의 하중전달에 관한 연구

최광호 남서울대학교 2001 남서울대학교 논문집 Vol.7 No.-

The composite frame system with reinforced concrete column and steel beam has some advantages in the structural efficiency by complementing the shortcomings between the two systems. The system, however, has also a lot of problems in practical design and construction process due to the material dissimilarities. Considering these circumstances, this research is aimed at the development of the composite structural system which enables the steel beams to be connected to the R/C columns with higher structural safety and economy. Basically the proposed connection system is composed of four split tees, structural angles reinforced by stiffener, high strength steel rods, connecting plates and shear plates. The structural tests have been carried out to verify the moment transfer mechanism from beam flange to steel rods or connecting plates through the angle reinforced by siffener. The four prototype specimens have been tested until the flange of beam reached the plastic states. From the tests, no distinct material dissimilarities between concrete and steel have been detected and the stress transfer through wide flange beam - structural angle - high strength steel rod or connecting plate is very favorable.