http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
( Soon Man Yoon ),( Do Hoon Kim ),( Mi Young Do ),( Byong Duk Ye ),( Jeong Sik Byeon ),( Suk Kyun Yang ),( Jin Ho Kim ),( Seung Jae Myung ),( Hyun Mi Kim ),( Kyeong Soon Park ),( Kwang Meyung Kim ),( 대한소화기학회 2007 SIDDS Vol.9 No.-
Background/Aims: Early detection of small primary tumors remains the mainstay of successful cancer therapy and improves survival rates. Matrix metalloproteinases (MMPs) revealed to participate in the early stages of tumorigenesis and primary tumor growth. The aim of the current study was to develop an efficient animal model for early and small colon tumors and assess the feusibility of protease activatable probes to detect early and small tumors. Methods: We used a mouse model for colon tumor by a combined treatment with azoxymethane (AOM) and dextran sodium sulfate (DSS). Male BALB/c mice (n=8, age 5 weeks) were given a single intraperitoneal administration (10 mg/kg body weight) of a colon carcinogen, AOM, followed by 2% DSS in drinking water for 7 days, and there-after they received no further treatment for up to 14 weeks. At week 16, all mice were injected via tail vein with MMP reporter probe (MMPSenseTM680, VisEn, Medical, Inc., MA, USA) followed by surgical exposure of the colon. Excised colons were evaluated with fluorescence imaging, histologic examination, and immunohistochemistry. Results: All mice treated with AOM and DSS developed colon tumors in distal colon with a multiplicity (no. of tumors/mouse) of 7.9±2.1. Fluorescence imaging demonstrated that all tumors were visible with the protease activatable probe. A target-to-background ratio (TBR) of 5.61 for tumor to adjacent normal mucosa was achieved. Histopathologically, all colon tumors revealed adenocarcinoma with or without adenoma. Results of immunohistochermistry confirmed MMP overexpression in the tumor compared with adjacent mucosa. Conclusions: The current experimental study shows that the expression of MMP is up-regulated in the mouse colon tumors induced by AOM and DSS, and highly matched with fluorescence imaging by a protease activatable probe. This strategy can be used to detect early stage tumors and may be used for early diagnosis of early and small tumors.
김명국,김민정,김광수,유경무 고신대학교 의학부 1999 高神大學校 醫學部 論文集 Vol.14 No.1-2
Background : Carcinomatous meningitis is a rare and often devastating complication in solid tumor patients. Methods : We reviewed 12 cases of leptomeningeal carcinomatosis seen at the Kosin University Gospel Hospital from Mar. 1993 to Jul. 1999. Leptomeningeal carcinomatosis were comfirmed by characteristic clinical symptoms, abnormal neuroimaging findings, elevated cerebrospinal fluid (CSF) carcinoembryonic antigen (CEA) titer, and positive CSF cytology. Results : The primary cancers of leptomeningeal carcinomatosis were lung cancer, stomach cancer, rectal cancer, and breast cancer. In one case the primary site was not identified. Clinical manifestations were headache, nausea, vomiting, and nuchal rigidity. The positive yields in the diagnosis of leptomeningeal carcinomatosis were 88.9% in CSF CEA titer, 72.7% in CSF cytology, and 58.3% in neuroimaging. Mean survival after diagnosis was 19 days with conservative treatment, and 44 days with intrathecal chemotherapy. Conclusions : For early diagnosis of lepto eningeal carcinomatosis, it is important to consider CSF CEA titer as well as CSF cytology.
Evaluation of the Anti-Tumor Effects of Paclitaxel-Encapsulated pH-Sensitive Micelles
Han, Jong-Kwon,Kim, Min-Sang,Lee, Doo-Sung,Kim, Yoo-Shin,Park, Rang-Woon,Kim, Kwang-Meyung,Kwon, Ick-Chan The Polymer Society of Korea 2009 Macromolecular Research Vol.17 No.2
We evaluated the efficacy of pH-sensitive micelles, formed by methoxy poly(ethylene glycol)-b-poly($\beta$)-amino ester) (PEG-PAE), as carriers for paclitaxel (PIX), a drug currently used to treat various cancers. PTX was successful encapsulated by a film hydration method. Micelles encapsulated more than 70% of the PTX and the size of the PTX-encapsulated micelles (PTX-PM) was less than 150 nm. In vitro experiments indicated that the micelles were unstable below pH 6.5. After encapsulation of PTX within the micelles, dynamic light scattering (DLS) studies indicated that low pH had a similar demicellization effect. An in vitro release study indicated that PTX was slowly released at pH 7.4 (normal body conditions) but rapidly released under weakly acidic conditions (pH 6.0). We demonstrated the safety of micelles from in vitro cytotoxicity tests on HeLa cells and the in vivo anti-tumor activity of PTX-PM in B16F 10 tumor-bearing mice. We concluded that these pH-sensitive micelles have potential as carriers for anti-cancer drugs.
Near-Infrared Fluorescence Imaging Using a Protease-Specific Probe for the Detection of Colon Tumors
( Soon Man Yoon ),( Seung Jae Myung ),( Byong Duk Ye ),( In Wha Kim ),( Nam Gon Lee ),( Yeon Mi Ryu ),( Kyeong Soon Park ),( Kwang Meyung Kim ),( Ick Chan Kwon ),( Young Soo Park ),( Chan Sik Park ),( 대한소화기기능성질환·운동학회 2010 Gut and Liver Vol.4 No.4
Background/Aims: Early tumor detection is crucial for the prevention of colon cancer. Near-infrared fluorescence (NIRF) imaging using a target-activatable probe may permit earlier disease detection. Matrix metalloproteinases (MMPs) participate in tumorigenesis and tumor growth. The aim of this study was to determine whether NIRF imaging using an MMP-activatable probe can detect colon tumors at early stages. Methods: We utilized two murine colon cancer models: a sporadic colon cancer model induced by azoxymethane (AOM), and a colitis-associated cancer model induced by a combination of AOM and dextran sodium sulfate (DSS). Colonic lesions were analyzed by histologic examination, Western blotting, immunohistochemical staining, and NIRF imaging using an MMP-activatable probe. Results: Multiple variable-sized tumors developed in both models and progressed from adenomas to adenocarcinomas over time. At the early stage of the AOM/DSS model, diffuse inflammation was observed within the tumors. MMP expression increased progressively through normal, inflammation, adenoma, and adenocarcionoma stages. NIRF signal intensities were strongly correlated with each tumor stage from adenoma to adenocarcinoma. NIRF imaging also distinguished tumors from inflamed mucosa. Conclusions: NIRF imaging using a protease-activatable probe may be a useful tool for early tumor detection. This approach could translate to improve the endoscopic detection of colon tumors, especially in patients with inflammatory bowel disease. (Gut Liver 2010;4:488-497)