PWM을 이용한 화재 감지용 자외선 센서의 구동 방법

고낙용,최한수,김광진,임병현 조선대학교 에너지.자원신기술연구소 2004 에너지·자원신기술연구소 논문지 Vol.26 No.1

We propose driving method of Ultraviolet sensor for fire alarms using pulse width modulation that is used to fire detector. Ultraviolet sensor is UV Tron using gas multiplication effect to current discharge and photoelectric effect of metal. To have high sensibility and to gain proper output voltage. it should have highly responsive performance. This research designed a driving circuit with UV sensors and proposed a method for false alarm reduction. The result shows the prevention and extinction of fire technique degree, certificated operation of detector.

제2형 당뇨병 동물모델인 OLETF 쥐에서 장기간 라미프릴 투여가 내당능 및 췌도 베타세포에 미치는 효과

고승현,윤건호,김명미,안유배,송기호,유순집,손현식,차봉연,이광우,손호영,강성구 대한당뇨병학회 2002 Diabetes and Metabolism Journal Vol.25 No.6

연구배경:최근 소개된 HOPE(Heart Outcomes Prevention Evaluation)study의 결과에 의하면, 심혈관질환이나 이에 대한 위험인자가 1가지 이상인 9,541명을 대상으로 안지오텐신 전환효소 억제제의 일종인 라미프릴을 투여하여 사망율과 뇌졸증, 심부전, 당뇨병 합병증이 개선되었을 뿐 아니라 다른 항고혈압제에 비해 새롭게 당뇨병으로 진단되는 환자가 줄었음을 보고한바 있다. 이에 저자들은 라미프릴(ramipril)을 제2형 당뇨병 동물모델에 장기간 투여함으로써 당뇨병을 예방하거나 고혈당 상태 또는 인슐린 감수성을 개선시킬수 있는지를 알아보고자 하였다. 방법:생후 24주된 체중 400∼450g의 OLETF와 LETO 쥐를 대상으로 6개월간 라미프릴 경구투여 하였고 혈압, 24시간 단백뇨 및 인슐린 내성검사를 시행하였다. 6개월 이후로는 8주간 투여군과 대조군의 체중을 비슷하게 맞추어 내당능 상태를 평가하였고 이후 다시 8주간 고농도 포도당을 투여하였다. 경구당부하검사를 시행한 후 희생시켜 조직표본을 만들어 guinea pig anti­human insulin 항체로 염색후 DAB로 발색하여 point count 법으로 베타세포를 정량하였고, 면역염색으로 췌도의 변화와 섬유화의 정도를 관찰하였다. 결과:라미프릴을 6개월간 투여하였을 때 OLETF 쥐에서 1)체중 증가가 적었으며 2)경구 당부하검사시 포도당하 면적은 의미있게 감소하였고 3)인슐린 내성검사상 Kitt가 증가되는 경향을 보였으며 4)수축기, 이완기 혈압과 24시간 단백뇨양이 의미있게 감소하였고 5)체중을 맞추면 내당능 상태가 비슷해졌다가 고농도 포도당 주입시 다시 포도당하 면적이 대조군에서 더 증가되었다. 라미프릴 투여군에서 췌도변형 및 파괴와 탈과립, 췌도내 섬유화가 감소하였다. 결론:본 실험에서는 인슐린 비의존성 당뇨병 동물 모델인 OLETF 쥐에서 당뇨병 발생 이전 단계부터 장기간 라미프릴을 투여할 경우 체중증가가 적었고, 투여군과 대조군의 체중을 맞추었을 때는 내당능상태의 차이가 없아가 고혈당 투여시 다시 투여군과 대조군에서 의미있는 차이를 보였다. 라미프릴 투여가 베타세포의 기능면에서 이로운 효과를 보일 것으로 생각되었다. 따라서 비만한 환자에서 안지오텐신 전환효소 억제제의 장기투여는 단백뇨 감소, 혈압강하 효과 이외에 체중증가의 억제 및 당뇨병 발생을 억제할 가능성을 시사한다. Background : In a Heart Outcomes Prevention Evaluation HOPE study, ramipril, a long-acting angiotensin-converting enzyme (ACE) inhibitor, significantly reduced the death rates the number of myocardial infarctions, strokes, heart failure as well as the risk of complications related to diabetes and of diabetes itself. However, it is known that ACE inhibitors improve glucose tolerance or insulin sensitivity or reduce the incidence of diabetes. Methods : 24 week-old OLETF (Otsuka Long Evans Tokushima Fatty) rats weighing 400 ~ 450 g were used in this study. 4 groups of rats were examined in parallel for 40 weeks. The OLETF rats were randomized for treatment with an aqueous solution of ramipril (5 mg/Kg) daily [OL (RMP), n=10)] and with saline [OL (CON), n=10)]. The LETO rats were also randomized in the same was as the OLETF rats (LT(RMP), n=10, LT (CON), n=10). The blood glucose level, body weight, systolic and diastolic blood pressure was assessed every month. At 3 and 6 months, the 24hrs urinary protein concentration was measured, and as insulin tolerance test and oral glucose tolerance test were conducted in all experimental groups. After 6 months, the body·weight was matched for 2 months in each corresponding group. Subsequently, a 15% sucrose loading was done for 2 months. After the glucose tolerance test, the pancreas was excised and immuno histochemical staining was conducted for insulin to quantify the beta cell mass by a point-counting method. In addition, the islet morphology was evaluated in the pancreas. Results : Ramipril treatment for a period of 6 months improved the 2hr blood glucose level, the area under the glucose curve in the oral glucose tolerance test, insulin sensitivity in addition to lowering significantly systolic and diastolic blood pressure and 24hrs urinary protein level significantly in OLETF rats. Of note, a lower weight gain was observed in both the ramipril-treated animals at 6 months. After weight matching, the AUC g and 2hr blood glucose level values were similar between the corresponding groups, but a 15% sucrose loading worsened the AUC g value. Histologically, the islets were less disorganized and the extent of fibrosis was lower in the ramipril-treated OLETF rats in the trichrome stain. Conclusion : Long-term treatment of ramipril, a long acting angiotensin-converting enzyme inhibitor may be useful for suppressing weight gain and proteinuria in addition to having a protective effect on the islet to harmful stimuli such as hyperglycemia (J Kor Diabetes Asso 25:469~482, 2001).

烏梅(Mume Fructus)水性엑스의 家兎 腸運動에 미치는 影響

高錫太,朴炫貞,任光楠 조선대학교 약학연구소 1994 藥學硏究誌 Vol.16 No.1

Effect of .Mume Fructus on intestinal function was investigated in rabbit, making use of its water extract. Mume Fructus water extract(MWE), of small doses(0.1, 0.3㎎/ml) produced contractile action but did not effect the spontaneous movement strips of high dose(1.0㎎/ml) elicited relaxative action and the inhibition of spontaneous movement in ileum strips. In lieum strips, MWE did not influence the contractile action but augmented the spontaneous movement to acetylcholine, MWE elicited the inhibition of contractile action and the agumentation of spontaneous movement to pilocarpine, MWE inhibited the both actions to histamine, did not affect to serotonin and blocked to dopamine. MWE exhibited the relaxation and the inhibition of spontaneous movement in longitadinal muscle of colon. In longitudinal muscle of colon, MWE inhibited both actions to acetylcholine, did not influence the contractile actions but augmented the spontaneous movement to histamine and serotonin. MWE elicited the increase of contractile actions and the decrease of spontaneous movement in circular muscle of colon. In circular muscle of colon, MWE augmented both actions to acetylcholine, inhibited both actions to serotonin, and then did not affect the contractile action but augmented the spontaneous movement to histamine.

Pulmonary Echinococcosis - A Case Report -

고일향,이진,구본일,손광현 인제대학교 1986 仁濟醫學 Vol.7 No.1

Pulmonary Echinococcosis (폐포충증)은 아직까지는 우리나라에서 보기드문 질환인 바 근래 국제 교류가 빈번해지면서부터 문제가 되기 시작하고 있으며, 특히 중동 지역에서 근무한 병력이 있는 환자에서 발생한 국내 보고가 수 예 있을 뿐이다. 저자들의 수술 치험 1례를 보고하는 바이다. Echinococcosis (hydatid disease) is endemic to the Mediterranean region, South America, Australia, and New Zealand where livestock, especially, sheep, are kept. The disease is considered to be rare in this country. However, recently, with increasing numbers of Koreans visiting those countries where hydatid disease is endemic especially Mid East region, the reports of hydatidosis are increasing, in the liver, but very rarelly in the lung. Authors experienced a case of pulmonary hydatid disease in the 30 year old male which was removed surgically on July 1985. We report the case to emphasize the need to consider this condition adding to other differential diagnosis of the mass lesion in the lung in our country and the literatures reviewed.

농산물 전자상거래 교육을 위한 농업용 E-Business 시스템 구현

고광현,김종숙 한국농업정보과학회 2000 농업정보과학 Vol.1 No.2

World wide spread of internet technologies has resulted in economic development and social change in many countries. In particular, it has contributed in creating a cyber shopping system in which fast transactions in commerce are made possible. The electronic marketing system in the Korean agricultural sector is still in its infant stage of development. But in the near future I-business will become popular in agricultural marketing. The professional agrirultural industry managers have to be well prepared to adapt themselves to the challenges of E-commerce that is now being used in the global market. Thus, we established the I-business sys-tem that will open a virtual shop and operate it fer education and practice in Korea National Agricultural Col-lege(KNAC). The E-business system's main organization is made up of five modules. information management of agricultural products, marketing management, marketing analysis, consumers and stores' management and fifteen sub modules of these modules. Complementary to these, we included Webpage designing for agricultural products. We constructed DB with Microsoft Access DB and SQL. We used DHTML(based on ASP, Java Script and CSS on Windows NT and 98) to make our home page. Also we gave EC's decicated server and If address and connected interact with "shop.kn.ac.kr" by E1 Line(2048Mbps). This system is designed primarily for stu-dents after graduation and practicing in their own farms. As such, it is also envisioned to become a big farmers' association shopping mall where individual Homepages of former students are connected with KNAC's main Homepage to facilitate easy access to various agricultural products. When the EC system expands, we plan to add more useful skills and develop new versions.

농업정보기술을 위한 ILP 프로세서에서 정·동적 분류를 이용한 결과 값 예측기

고광현,조영일 한국농업정보과학회 2003 농업정보과학 Vol.4 No.1

Recent works have addressed that the limit imposed by data dependencies could be overcome to some extent by the data value prediction. A hybrid value predictor could obtain high prediction accuracy using advantages of various predictors, however it had a defect that identical instructions had overlapping entries in all predictors. In this paper, we proposed a new hybrid value predictor which achieved high performance by using information of static and dynamic classification. The proposed predictor could enhance prediction accuracy and efficiently decreased prediction table size of the predictor, because it allocated each instruction into single best-suited predictor during a fetch stage by using information of static classification. Simulation results based on the SimpleScalar/PISA tool set and the SPECint95 benchmarks showed the average correct prediction rate of 86.3% by using the static classification mechanism. Also, we achieve the average correction prediction rate of 87.9% by using static and dynamic classification mechanism.

유기용제 노출에 따른 신발제조업체 근무 여성근로자들에서의 후천적 색각이상

변주현,이광영,김영기,고광욱,이용환 대한산업의학회 2001 대한직업환경의학회지 Vol.13 No.3

목적 : 복합유기용제에 노출되는 신발제조업체 근무 여성근로자들에서 유기용제 누적노출량과 후천성 색각이상 발현빈도간의 상관성을 조사하기 위해 본 연구를 실시하였다. 방법 : 신발제조업체 근무 여성근로자들 가운데 유기용제에 노출되는 85명과 유기용제에 노출되지 않는 근로자 88명을 대상으로 조사하였다. 조도가 표준화된 방에서 시력검사와 색각이상 검사를 실시하였다. 작업 부서를 접착, 사상 및 선처리 부서로 나누었고 작업 부서별로 현재노출량과 누적노출량을 구하였다. 결과 : 색각이상의 유병률은 노출군이 대조군보다 통계학적으로 유의하게 높았으며 (P=0.017). 유기용제 노출군 85명 가운데 후천적 색각이상인 청황색 색각이상자가 5명(5.9 %)이었다. 안증상도 노출군이 대조군보다 발생빈도가 높았으며 통계학적으로 유의한 차이를 보였다(P=0.001). 색 혼란지수에 영향을 줄 수 있는 변수들인 연령, 교육기간, 누적노출량 등은 유의한 변수로 나타나지 않았다. 결론 : 유기용제 노출군에서 색각이상의 유병률이 대조군보다 유의하게 높았으며, 특히 유기용제 노출군에서 후천적 색각이상인 청황색 색각이상도 발견되었다. 그러나 유기용제 누적노출량과 색 혼란지수와 유의한 연관성은 발견할 수 없었는데 이는 유기용제의 노출량이 너무 낮았으며, 근로자의 이직률 또한 놓아 누적노출량 계산을 위한 추적기간이 제한되었기 때문인 것으로 생각되며 앞으로 고농도 노출 근로자를 대상으로 연구를 하면 분명한 결과를 얻을 수 있을 것으로 기대된다. Objectives : This study was undertaken to investigate the relationship between color vision defects and occupational exposure to mixed organic solvents in women workers who were engaged in the shoe manufacturing industry. Methods : A total of 173 women workers were involved in this study, 85 of whom were exposed to mixed organic solvents and were defined as the exposed group, and 88 workers comprised the non-exposed control group. The patients were questioned as to their drinking history, the year in which they were exposed and whether they had eye symptoms of blureed vision, eye fatigue, and eye irritation. The exposed workers in the shoe manufacturing factory were engaged in 3 work areas which were pasting, trimming, and cleaning. Their Color vision was assessed using the Hahn's double 15 hue test under standard illumination and their current and cumulative exposure levels were measured. Results : The prevalence of color vision defect was 21.2% in the exposed group and 8.0% in the control group, and the blue-yellow defect was found to be 5.9% in the exposed group. The logistic regression for the acquired dyschromatopsia and color confusion index showed that there were no variables that had significant relationships. Eye symptoms were more frequently developed in the exposed group. Conclusions : There is a possibility of developing color vision defects when workers are exposed to mixed organic solvents. However, the results of the color confusion index showed that there was no significant relationship to the coumulative exposure level, because of the low exposure level and high occupational turn over rate of the workers. Because the workers in the present study were exposed to low level solvents it will be necessary to study workers exposed to higher levels of organic solvents.

합성피혁제조업체 근로자들의 요중 N-Methylformamide 농도의 계절적 변이

이광영,변주현,송혜란,김진하,고광욱,이용환 大韓産業醫學會 2003 대한직업환경의학회지 Vol.15 No.2

목적: 본 연구는 여름과 겨울의 계절적 차이에 따른 디메틸포름아미드의 체내 흡수의 변화를 알아보고자 시행되었다. 방법: 일개 합성피혁제조업체에서 직접적으로 디메틸포름아미드를 취급하는 22명의 근로자들을 대상으로 2001년 2월(겨울)과 7월(여름)에 작업환경측정을 통해 기중 DMF농도를 측정하였고 특수건강검진을 실시하여 요중 NMF의 배설량을 측정하고 간기능 검사를 시행하였다. 또한 검사 당일의 평균 기온과 평균 습도를 측정하고 설문지를 통해 알코올 음주 정도, 피부 질환의 유무, 보호구 착용 유무 등을 조사한 후 여름과 겨울의 자료를 비교 분석하였다. 결과: 겨울과 여름의 평균 공기 중의 디메틸포름아미드 기하 평균 농도는 각각 11.55ppm, 13.78ppm으로 통계적으로 유의한 차이는 없었으나 요중엔-메틸포름아미드 배설량의 기하 평균은 겨울이 31.23㎎/g creatinine인 것에 비해 여름이 96.09㎎/g creatinine으로 겨울에 비해 여름의 요중 엔-메틸포름아미드 배설량이 유의하게 높았다(p<0.05). 공기 중 디메틸포름아미드 농도(ppm)에 대한 요중 엔-메틸포름아미드 배설량 (㎎/g creatinine)의 비는 여름(1:6.97)이 겨울(1:2.70)에 비해 약 2.6배 정도 높았다. 결론: 여름과 겨울의 요중 NMF배설량의 차이는 아마도 연구 대상 사업장의 환기 시설이 미약하고 근로자들이 DMF에 적합한 보호구를 적절하게 사용하지도 않았기 때문이고, 무엇보다도 높은 온도와 습도에서 짧은 옷을 입고 근무함으로써 피부 노출면적이 증가되어 DMF의 피부 흡수의 양이 많아졌기 때문으로 판단된다. Objectives: This study was carried out to identify seasonal variations of urinary concentrations of N-methylformamide(NMF) among workers employed at a synthetic leather factory. Methods: Study subjects consisted of 16 male and 6 female workers who were involved in the direct treatment of dimethylformamide(DMF) in a synthetic leather factory. By using health examination data and the results of air measurements and biologic monitoring conducted in February and July, 2001, we identified seasonal variations of the DMF concentrations in the air and NMF concentrations in urine. Results: 1) In winter and summer, average temperatures at the working sites were 3.2℃ and 26.5℃, respectively and average humidities were 35.4% and 84.5%, respectively. 2)Airborne DMF concentrations were not significantly different between summer(13.78ppm) and winter(11.55ppm). 3)NMF concentrations in urine were found to be significantly higher in summer(96.09㎎/g creatinine) than in winter(31.23㎎/g creatinine) (p<0.001). Conclusions: The seasonal difference in the urinary excretion values of NMF may be due to increased dermal absorption of DMF with the higher ambient temperature and humidity in summer and the increased area of exposed skin.

임신부 뇨로부터 정제된 인간 상피세포 증식 인자 유사체의 in vitro bioassay 및 특성

박세철,전재현,남정현,권태종,고인영,유광현 한국미생물생명공학회 ( 구 한국산업미생물학회 ) 1996 한국미생물·생명공학회지 Vol.24 No.4

벤조산흡착, 음이온 교환수지, 단일클론항체을 이용한 immunoaffinity chromatography를 통하여 임신부 뇨로부터 천연의 hEGF를 정제하였다. 정제된 hEGF는 μ Bonda C_18 column을 사용한 HPLC 분석을 통하여 4개의 fraction으로 분리가 가능하였으며 western blot과 double immunodiffusion 실험 결과, 각각의 fraction이 hEGF의 특성을 가진 유사체인 것을 알 수 있었다. 또한 hEGF 표준 물질과의 spiking 및 아미노산 분석 등을 통하여 두 번째 fraction이 nhEGF와 동일한 것으로 확인하였다. nhEGF 및 그 유사체의 생물학적 활성 비교를 위하여 NIH 3T3 세포주에서 5'-Brdu incorporation 측정을 위한 labelling 시간, 혈청 농도의 최적 조건을 결정하였다. NIH 3T3 세포주의 DNA 합성능은 0.2% FCS가 포함된 저혈청 배지에서 hEGF가 0.1~10 ng/ml 농도로 첨가하였을 때 증가하는 경향을 나타냈다. HPLC를 통하여 분리된 두번째 hEGF 유사체가 다른 유사체보다도 생물학적인 활성이 우수하였으며, rhEGF 표준물질과의 spiking 및 아미노산 서열 분석등을 통하여 nhEGF로 밝혀졌다. 임신부의 뇨의 hEGF 유사체 함량중 natural hEGF는 46%이었다. Natural human epidermal growth facto (nhEGF) was purified from pregnant human urine by benzoic acid adsorption, DEAE-Sepharose ion exchange, and immunoaffinity chromatography. The purified nhEGF was further separated into four fractions using Bondapak C_18 HPLC system. Following characterization by Western blot and double immunodiffusion, we found that each fraction corresponds to four derivatives of the nhEGF. For biological analysis of nhEGF, we optimized the labeling time and serum concentration for the incorporation of 5-bromo-2'-deoxy-uridine (BrdU), a non-radioactive alternative for [^3H]-thymidine uptake, into NIH 3T3 cells. The DNA synthesis of NIH 3T3 cells was gradually increased at the nhEGF concentrations between 0.1~10 ng/ml in the Dulbecco's Modified Eagles Medium (DMEM) containing 0.2% Fetal calf serum (FCS). When we assayed the biological activity of four fractions, the activity of the second fraction was superior to that of the others.

烏貝散이 흰쥐 胃의 Gastrin, Histamine, Somatostatin 면역반응세포에 미치는 영향

이시섭,나현욱,고병문,이광규,이창현 대한동의병리학회 2001 동의생리병리학회지 Vol.15 No.5

To investigate the elects on the administration of Opae-san in rats. Opae-san (500mg/day) and omeprazole(10mg/day) were administration with stomach tube for 4 weeks and 8 weeks. This experiment were investigated numerical change of immunoreactive cells of gastric, histamine and somatostatin in rat stomach mucosa by the immunohistochemical method. The results were as follows : 1. In Opae-san administration group for 4 weeks, the number of gastric immunoreactive cells were increased in one and a half times than that of control group. In omeprazole administration group for 4 weeks, the number of gastric immunoreactive cells were increased in four times than that of control group. In Opae-san administration group for 8 weeks, the number of gastric immunoreactive cells were increased four times than that of control group. In omeprazole administration group for 8 weeks, the number of gastrin immunoreactive cells were increased in six times than that of control group. 2. In Opae-san administration group for 4 weeks, the number of histamine immunoreactive cells were increased in two times than that of control group. In omeprazole administration group for 4 weeks, the number of histamine immunoreactive cells were increased in six times than that of control group. In Opae-san administration group for 8 weeks, the number of histamine immunoreactive cells were increased three times than that of control group. In omeprazole administration group for 8 week, the number of histamine immunoreactive cells were increased in seven times than that of control group. These results suggest that Opae-san extracts inhibit the secretion of gastric acid and this extract use to therapeutic herb of gastric disorders related to the hyperacidity and gastric ulcer.