신부전이 동반된 당뇨병 환자에서 발생된 침습성 모균증 : 수술과 Liposomal amphotericin B 및 GM-CSF 병합 요법에 의한 성공적인 치험 1예

이원영,오기원,임국희,장재혁,이동건,최정현,강무일,신완식,차봉연,이광우,손호영,강성구 대한화학요법학회 1999 대한화학요법학회지 Vol.17 No.4

저자들은 신부전이 동반된 당뇨병 환자에서 발생된 부비동형 모균증에 대하여 수술과 함께 liposomal amphotericin B 및 GM-CSF의 복합치료를 하여 성공적으로 치료한 1예를 경험하였기에 문헌 고찰과 함께 보고하는 바이다. Mucormycosis (zygomycosis) primarily affects diabetic or immunocompromised patients and typically progresses rapidly, necessitating surgical excision and antifungal therapy with amphotericin B. Large doses of amphotericin B are needed for cure, but it has the risk of causing significant renal toxicity. The recent development of liposomal amphotericin B allows antifungal therapy to be administered with potentially improved efficacy and reduced nephrotoxicity. We have experienced a case of paranasal mucormycosis successfully treated with surgery, liposomal amphotericin B and GM-CSF. A 59-year-old male suffering from diabetes mellitus for 6 years was admitted with pain at left maxillary area. He was diagnosed as mucormycosis after cytologic exam on the necrotic nasal mucosa, which showed typical hyphae. He have had diabetic nephropathy with macroproteinuria and had rapidly rising serum creatinine levels with the amphotericin B treatment: creatinine levels reverted to basal level with the use of liposomal amphotericin B. Despite surgical excision and continued antifungal therapy, his infection was not effectively controlled. Therefore, GM-CSF was administered additionally to improve phagocytic activity of leukocytes. He was finally cured after receiving a combination of aggressive surgery, liposomal amphotericin B and GM-CSF. To our knowledge, this is the first detailed clinical description of the treatment of mucormycosis with liposomal amphotericin B in Korea.

原乳의 冷却方法과 市乳의 殺菌方法 및 貯藏方法에 따른 細菌學的인 變化에 관한 硏究

金秀光,李革新 건국대학교 1979 論文集 Vol.9 No.1

The experiment was carried out to compare Total Standard Plate Counts (S.P.C) according to Cooling Method of Raw Milk and Pasteurization Method of Market Milk, to examine Changing of Market Milk at 5℃ and room temperature during various Storage Period. They were investigated in S.P.C. and quality of Markt Milk. The results obtained were as follows; 1.Cooling Methods of raw milk: S.P.C. were 4.7×107 cells/ml in cooling method by ground water, 3.2×107 cells/ml in refrigerator storage method, both were above the law. Then 13% of 4.7×107 cells/ml in milk cooler method and 75% of 1.9×106 cells/ml in bulk tank method were below the law. 2.Pasteurization methods of market milk; Raw milk contained 2.4×107 cells/ml, then M.T.S.T milk contain 2.6×105 cells/ml, both was above the law. H.T.S.T. and holding mixed method and L.T.L.T. method were contained 2.0×102 cells/ml and 1.3×102 cells/ml, respectively both were below the law, 6.2×10cells/ml by V.H.T. method was very excellent among them. 3.Storage of market milk at 5˚C; S market milk after 4th days was 2.6×103cells/ml in September, 3.2×102 cells/ml in October, both were below the law, K market milk after 4 th days in September was 6.0×104 cells/ml, after second day in October was 1.2×105 cells/ml, but in spite of pasteurization SA control market milk in September was 2.5×105 cells/ml, after firstday in October was 4.4×104 cells/ml, all were above the law. All samples were normal in Acidity test, Alcohol precipitation teat and Organoleptic test. 4.Storage of market milk at room temperature (23-25℃) ; S market milk after 12 hours storage in September was 1.8×105 cells/ml, and after 24 hours in October was 1.0×106 cells/ml. K market milk both in September and October after 8 hours were 1.3×105cells/ml and 8.4×104 cells/ml respectively SA market milk pasteurized immediately in September and in October were 2.0×105 cells/ml, and 2.6×105 cells/ml, and both were above the law. All of market milk were 0.18~0.25% in Acidity test, positive in Alcohol test, and abnormal flavor in Organoleptic test after 24 hours.

중이강 내에 발생한 선천성진주종

김광수,박철규,이병돈,장혁순,강주원 순천향의학연구소 1995 Journal of Soonchunhyang Medical Science Vol.1 No.2

Congenital cholesteatoma is a rare disease which originated from the ectoderm that from the primitive notochord and embryonal cell rests. It may occur in any portion of the cranial bones. Various symptoms may develope about position of the lesions. If the lesion located in the middle ear, symptoms were showed conductive hearing loss and normal tympanic membrane. We have experienced two cases of congenital cholesteatomas which were located in the middle ear. We report these cases with review of literatures.

培地形態와 貯藏溫度 및 期間이 Str.lactis와 L.bulgaricus에 미치는 影響

金光洙,李革新 건국대학교 1979 論文集 Vol.9 No.1

The lactic acid bacteria were inoculated in original media as culture or washed cell form after 16 hours incubation in skimmilk or MRS broth and then production of lactic acid, etc. were investigated during 8 months frozen storage. The studies on the storage at low temperature after incubation in skimmilk and the use of additive (glycerol, skimmilk powder, lactose) to prevent injury of cells were carried out as accompanying treatments. The results obtained in this study were as follows. 1.The lactic acid production ability of L. bulgaricus after 8 months preservation at skimmilk medium was 0.72% in the culture treatment and 1.21% in washed cell treatment, but at MRS broth medium, it was 0.50% in culture treatment and 0.54% in washed cell treatment and shows the same trend at Str. lactis. It shows that skimmilk is better than MRS broth as a medium. 2.In washed cell treatment, the lactic acid production ability of Str. lactis after 8 months preservation was 0.26% in skimmilk medium and 0.57% in MRS broth medium, and in culture treatment it was 0.21% in skimmilk and 0.31 % in MRS broth. The same trend was shown in L, bulgaricus. 3.The activity of Str. lactis after 8 months frozen storage as washed cell form was better than of 18 days storage at 4℃. 4.In additive, the viability of lactic acid bacteria after 7 months preservation was 2.76×108 in glycerol 1.2×108 in skimmilk and 0.79×108 in lactose.

자동영상추적장치를 이용한 카드뮴 처리 Oryzias latipes의 행위독성연구

류지성,이철우,최필선,최성수,류홍일,이길철,정규혁,박광식 한국환경독성학회 1999 환경독성보건학회지 Vol.14 No.4

Changes in certain fish behaviors are known to be very sensitive indicators of sublethal exposure to environmental contaminants. Therefore, behavioral toxicity tests, if properly designed, can be very useful to assess the influence of hazardous chemicals on fish. However, quantitative analysis of xenobiotic -mediated changes in locomotor behavior in fishes are rare, due mainly to the methodological difficulties. In general, fish movement has been known to be hypertrophic or hypotrophic according to the chemicals. As a study of fish behavioral toxicities, we quatified the swimming movement of Orydas latipes using computer-automated video tracking system. Oryzias latipes was exposed to cadmium of 128 mg/L for 1 hour in a limited aquaria, then the total swimming distance, the average swimming velocity, the histogram of turning angles, and the turning frequency were analyzed. Fish treated with cadmium showed decreased swimming activities, decreased velocity, and decreased turning frequency, which means hypotrophic activity. From these results, the computer-automated video tracking system of this study seems to be a good tool for the evaluation of the potential ecotoxicological studies.

공정변수의 변동을 고려한 호감도 함수를 통한 다중반응표면 최적화

권준범,이종석,이상호,전치혁,김광재 한국경영과학회 2005 한국경영과학회지 Vol.30 No.1

A desirability function approach to a multiresponse problem is proposed considering process parameter fluctuation which may amplify the variance of response. It is called POE(propagation of error), which is defined as the standard deviation of the transmitted variability in the response as a function of process parameters. In order to obtain more robust process parameter setting, a new desirability function is proposed by considering POE as well as distance-to-target of response and variance. The proposed method is illustrated using a rubber product case in Ribeiro et al. (2000).

흰쥐에서 음경발기 평가의 척도로서 음경해면체내압측정술 확립

송윤섭,김용준,이광우,김준모,문기혁,박영호,민영기,유형균,김형건 순천향의학연구소 2001 Journal of Soonchunhyang Medical Science Vol.7 No.1

Purpose: Because studies about the control of penile erection at the entral nervous system was dependent on the animal experiments, establishment of measuring penile erection is important to evaluate the effects changes at the central nervous system on the penile erection. Intracavernous pressure measurement in rats has been introduced as a suitable index for the evaluation of penile erection but this method is invasive and technically difficult. So, we established the intracavernous pressure measurement in rats as the experimental index for penile erection. Materials and Methods: 42 male adult Sprague-Dawley rats (250-300gm) were divided to saline or papaverine treated group. Rats were placed on a heating table to maintain their body temparature and anesthetized with 50mg/kg of pentobarbital sodium by intraperitoneal injection. A cannula was inserted to the left carotid artery to measure systemic blood pressure. Saline (0.05, 0.1ml), papaverine(0.05, 0.1, 0.25, 0.5, 1.0, 2.0mg) were injected intracavernously via 26 guage needle filled with saline or papaverine which connected to pressure transducer and polygraph was inserted into the corpus cavernosum on one side to inject the drug and to measure intracavernous presure. Results: Properly executed insertion and intracavernous administration produced a instantaneous but transient rise in intracavernous pressure that substantially stabilized and maintained at 5.4±0.4mmHg. Intracavernous pressure and duration of penile blood flow following intracavernous injection of papaverine are increased comared to those of saline. Conclusions: Monitoring intracavernous pressure in rats represents a suitable index for the evaluation of penile erection in small laboratory animals.

Humic acid가 카드뮴 어독성에 미치는 영향

류지성,정규혁,최필선,이길철,최덕일,최성수,류홍일,박광식 한국환경독성학회 1998 환경독성보건학회지 Vol.13 No.1

Cadmium, a major aquatic pollutant in many parts of the world, is toxic and readily accumulated in aquatic organisms. It mainly exists in water as complexes with organic ligands such as dissolved organic carbon and this complexation is known to have effects on the aquatic toxicities. In this study, acute toxicity, histology, and bioaccumulation were studied to evaluate effects of humic acid on cadmium toxicity and bioavailability to fish using Oryzias latipcs and Cypriruts carpio. 96h~LC50 of cadmium was 6.38 mg/L using Oryzias latipes. However, the mortality showed the dose-dependent decrease when humic acid was added to the test solution. When fish were exposed to 5 mg/L of cadmium, histological changes in the exposed organs (caudal fins, gills, kidney, liver, and gut) of Cyprinifs carpio were decreased by humic acid, especially in kidney and liver. Bioaccumulation of cadmium also decreased by treatment of humic acid. It seems that the formation of complexes between cadmium and humic acid may decrease bioavailability of cadmium to fish, and thus reduce the toxic effects of cadmium.

Humic acid가 카드뮴 어독성에 미치는 영향

류지성,정규혁,최필선,이길철,최덕일,최성수,류홍일,박광식 성균관대학교 약학연구소 1998 成均藥硏論文集 Vol.10 No.1

Cadmium, a major aquatic pollutant in many parts of the world, is toxic and readily accumulated in aquatic organisms. It mainly exists in water as complexes with organic ligands such as dissolved organic carbon and this complexation is known to have effects on the aquatic toxicities. In this study, acute toxicity, histology, and bioaccumulation were studied to evaluate effects of humic acid on cadmium toxicity and bioavailability to fish using Oryzias latipes and Cyprinus carpio. 96h-LC50 of cadmium was 6.38 ㎎/L using Oryzias latipes. However, the mortality showed the dose-dependent decrease when humic acid was added to the test solution. When fish were exposed to 5㎎/L of cadmium, histological changes in the exposed organs(caudal fins, gills, kidney, liver, and gut) of Cyprinus carpio were decreased by humic acid, especially in kidney and liver. Bioaccumulation of cadmium also decreased by treatment of humic acid. It seems that the formation of complexes between cadmium and humic acid may decrease bioavailability of cadmium to fish, and thus reduce the toxic effects of cadmium.

전국 10개 종합병원 중환자실 환자의 직장내 Vancomycin-resistant Enterococci(VRE) 보균율, 보균의 위험요인 및 유전적 다양성 조사

정희진,송준영,엄중식,김우주,최선주,최정현,이혁,정문현,이광미,신동현,손보라,최종성,우흥정,권정아,이경원,최강원,박승철 대한감염학회 2002 감염 Vol.34 No.5