Ki-67 labeling index as a prognostic marker in advanced stomach cancer

Sang Hyuk Seo,Kwang Hee Kim,Sang Hoon Oh,Yunseon Choi,Ki Jung Ahn,Ji Young Lee,Sang Min Lee,박지선,Woo Gyeong Kim 대한외과학회 2019 Annals of Surgical Treatment and Research(ASRT) Vol.96 No.1

Purpose: Proliferation marker Ki-67 is widely used in cancer prognosis prediction. We tried to investigate the role of Ki-67 as a prognostic factor in stomach cancer after surgery in this study. Methods: We retrospectively evaluated 251 patients who underwent curative resection for gastric cancer from 2010 to 2015. In pathologic examination, Ki-67 labeling index was defined as the percentage of Ki-67 antigen positive cells. Prognostic significance of Ki-67 for gastric cancer was evaluated. Disease-free survival (DFS) was assessed as a primary end-point. Results: The median follow-up period was 28.0 months. Thirty-one patients (12.4%) showed Ki-67 labeling index (LI) lower than 25%. Sixty-eight patients (26.6%) showed recurrence during follow-up period. Recurrence was associated with Ki- 67 LI level (≤25%, P = 0.016), and lymph node metastasis status (P = 0.002). High Ki-67 LI level (>25%) was also related to p53 positivity (P < 0.001) and poorly cohesive type (P = 0.002). The 3-year DFS was 69.4%. Low Ki-67 LI level (≤25%) was related with low DFS (47.6% vs. 72.6%, P = 0.016). T stage (P < 0.001), N stage (P = 0.006), lymphovascular invasion (P = 0.010), and neuronal invasion (P = 0.001) also affected the DFS. In addition, T stage (P = 0.03) and Ki-67 LI (P = 0.035) were independent prognostic factors for DFS. In patients treated with adjuvant chemotherapy (n = 239, 93.4%), low Ki-67 (≤25%) was a poor prognostic factor for DFS (P = 0.013). Conclusion: Low Ki-67 LI predicts high rate of progression and low DFS of stomach cancer. Ki-67 LI can be a predictive marker in resected stomach cancer treated with surgery and adjuvant chemotherapy.

Reconstruction of Rabbit Corneal Epithelium on Lyophilized Amniotic Membrane Using the Tilting Dynamic Culture Method

Ahn, Jae-Il,Lee, Doo-Hoon,Ryu, Yang-Hwan,Jang, In-Keun,Yoon, Mun-Young,Shin, Youn Ho,Seo, Young-Kwon,Yoon, Hee-Hoon,Kim, Jae-Chan,Song, Kye-Yong,Yang, Eun-Kyung,Kim, Ki-Ho,Park, Jung-Keug Blackwell Publishing Inc 2007 Artificial Organs Vol.31 No.9

<P>Abstract: </P><P>Rabbit corneal epithelium was reconstructed using tilting dynamic culture with a self-manufactured, amniotic membrane (AM) supporter and a lyophilized amniotic membrane (LAM). Rabbit corneal epithelial (RCE) cells were cultured and cryopreserved after isolation from the limbus. The second- and third-passage RCE cells were plated onto the epithelial side of the LAM of Ahn's AM supporter. Two days later, the air–liquid interface culture was maintained with third-passage RCE cells for 6 days and second-passage corneal epithelial cells for 9 days. The average viability of thawed RCE cells, assessed using trypan blue dye exclusion, was 77.42%. The reconstructed corneal epithelium was characterized by histological (hematoxylin and eosin) and immunohistochemical staining (proliferating cell nuclear antigen) for light microscopy, and by reverse transcriptase-polymerase chain reaction, glucose assay, and transmission electron microscopy. The basal layer of the reconstructed corneal epithelium was well formed, and the epithelium was tightly constructed due to the increase in cell proliferation and differentiation caused by the tilting dynamic culture, as opposed to static culture. Tilting dynamic culture was useful for the reconstruction of the epithelium using easily damaged epithelial cells and resulted in more stratum cell layers. Moreover, cytokeratin (CK3) mRNA expression in tilting dynamic cultured third-passage RCE cells seeded onto AM was greater than in static cultured third-passage RCE cells. The morphology of the reconstructed corneal epithelium on LAM by tilting dynamic culture for 9 days resembled that of the skin epidermis. This was thought to be because the tilting dynamic culture not only accelerated the proliferation and differentiation of cells by physical or mechanical stimulation, but also ensured that the supply of medium was delivered to the basal cells more efficiently. Thus, the reconstruction of the corneal epithelium using LAM and tilting dynamic culture was considered to be a good in vitro model for autologous or allogeneic transplantation of corneal epithelium and skin epidermis in patients with damaged epithelia. </P>

Cell proliferation and neuroblast differentiation in the rat dentate gyrus after intrathecal treatment with adipose-derived mesenchymal stem cells.

Choi, Jung Hoon,Chung, Jin Young,Yoo, Dae Young,Hwang, In Koo,Yoo, Ki-Yeon,Lee, Choong Hyun,Yan, Bing Chun,Ahn, Jin Ok,Youn, Hwa Young,Won, Moo-Ho Kluwer Academic/Plenum Publishers 2011 Cellular and molecular neurobiology Vol.31 No.8

<P>Mesenchymal stem cells (MSC) have emerged as a new therapeutic tool for a number of clinical applications, because they have multipotency and paracrine effects via various factors. In the present study, we investigated the effects of adipose-derived MSC (Ad-MSC) transplantation via intrathecal injection through the cisterna magna on cell proliferation and differentiation of endogenous stem cells in the hippocampal dentate gyrus (DG) using Ki-67 (a marker for proliferating cells), and doublecortin (DCX, a marker for neuroblasts). The transplanted Ad-MSC were detected in the meninges, not in the hippocampal parenchyma. However, the number of Ki-67-immunoreactive cells was significantly increased by 83% in the DG 2 days after single Ad-MSC injection, and by 67% at 23 days after repeated Ad-MSC treatment compared with that in the vehicle-treated group after Ad-MSC transplantation. On the other hand, the number of DCX-immunoreactive cells in the DG was not changed at 2 days after single Ad-MSC injection; however, it was significantly increased by 62% 9 days after single Ad-MSC injection. At 23 days after repeated Ad-MSC application, the number of DCX-immunoreactive cells was much more increased (223% of the vehicle-treated group). At this time point, DCX protein levels were also significantly increased compared with those in the vehicle-treated group. These results suggest that the intrathecal injection of Ad-MSC could enhance endogenous cell proliferation, and the repeated Ad-MSC injection could be more efficient for an enhancement of endogenous cell proliferation and differentiation in the brain.</P>

안검하수의 치료

안기영,백봉수,박효천 大韓成形外科學會 1985 Archives of Plastic Surgery Vol.12 No.1

The aim of this thesis is to study 20 cases of blepharoptosis who were followed-up among many patients who received ptosis surgery at Kyungpook National University Hospital from Jan. 1980 to April 1984. To obtain the best surgical results in blepharoptosis, it was necessary to select the appropriate operation form each case according to levator function, the amount of ptosis, its etiology, as well as other factors. In cases of 4.1mm or more levator function, the author attempted to resect the levator muscle, but in cases of between 3mm and 4mm, the combination of levator muscle resection and frontalis sling was selected. On the other hand, in cases of 3mm or less levator function the fontalis sling was selected combined with fascia lata. The results were as follows; 1. The ratio of congenital versus acquired ptosis was 17 : 3. 2. Among 17 cases of congenital ptosis, male versus female was 11 : 6, and monocular versus bi-ocular was 9 : 8. Among 9 cases of congenital monocular ptosis, Lt. versus Rt. eye incidence was 5 : 4. 3. Among 3 cases of acquired ptosis, the causes were traumatic, neurofibromatosis, and birth injury respectively in each separate case. 4. In 5 of the congenital cases, blepharophimosis was combined, and in 4 strabismus was observed. 5. In the amount of ptosis, 7 cased were belew 2mm, 3 cases between 2.1∼4,9mm, and 10 cases above 5mm. In the measurement of the lavator muscle function, 7 cases were belew 3.0mm, 2 cases between 3.1∼4mm, and 11 cases above 4.1mm. 6. The best results were obtained by the levator muscle resection: Which was done for the patients with more than 4.1mm of levator muscle function. Good to excellent results were obtained in 8 cases among 10 cases. 7. The combination of levator muscle resection and frontalis sling was done for the patients with levator muscle function of 3.1mm∼4mm, and gave poor results. 8. Undercorrection and lid fold deformity were the most common types, except for lid lag and lagophthalmos in sleeping.

시공간 트렐리스 부호화 CDMA 시스템에서의 선택적 PRC를 사용한 레이크 수신기의 성능 분석

기영민,임은정,안철용,김동구,이석규 延世大學校 電波通信共同硏究所 2002 電波通信論文誌 Vol.6 No.1

다중 경로 페이딩 채널에서의 시공간 트렐리스 부호 (STTC, Space-Time Trellis Codes)의 성능 열화를 보완하기 위한 시공간 등화기는 수신기를 복잡하게 하는 단점이 있다. 본 논문에서는 PRC (Principal Ratio Combining) 레이크 수신기를 제안하였다. 이 수신기는 다중 경로 신호를 PRC 기법을 사용하여 컴바이닝 함으로서 수신 SINR (Signal to Interference and Noise Ratio)을 개선하는 효과를 얻는다. 또한, 본 논문에서는 모든 다중 경로 컴바이닝에 비교하여, 낮은 복잡도로 비슷한 성능을 얻을 수 있는 선택적 PRC (Hybrid Selection / Principal Ratio Combining) 구조를 제시하였다. The space-time equalizer has been used to enhance the space time code in the multipath fading channel. However it requires higher receive complexity. In this paper, the principal ratio combining (PRC) receiver for space-time trellis coded CDMA system is proposed in the multipath fading channels. It maximizes the received signal to interference and noise ratio (SINR) and reduces the complexity of the system compared to optimum ML receiver. We also present the hybrid selection / principal ratio combining (HS/PRC) scheme that can achieve the performance comparable to that of the full path PRC combining system.

입원 환자 영양검색 전산 프로그램 개발

안수미,윤기영,신연명,최경현,임근승,장문경,송윤미,공은희 고신대학교의과대학 2008 고신대학교 의과대학 학술지 Vol.23 No.2

Background : About 30% of patients in the hospital are under nourishment. A large portion of people are undernourished when they are admitted to the hospital and in the majority of these, undernutrition develops further while in hospital. Patient-Generated subjective global assessment(PG-SGA), a method of nutritional assessment based on clinical judgment, has been widely used to assess the nutritional status of adults for both clinical and research purposes. Modified PG-SGA can be used as a nutrition screening tool, and a comprehensive nutrition assessment is desirable for those who are malnourished. The purpose of this study is to develop a simple, reliable and valid nutrition screening program(NSP). Methods : We applied the nutrition screening program(NSP) to adult inpatients who are at risk of malnutrition using the Modified Patient-Generated subjective global assessment(PG-SGA) and objective assessment(electrical medical record data). Results : Therefore simple and beneficial NSP is developed for patients with severe malnutrition. Using NSP. nutritional information of the severe malnutrition patient should be shared with the physicians and they should be taken care of by clinical dietitians to improve their nutritional status. Conclusion : The Modified PG-SGA and nutrition screening tool are simple, reliable and valid nutrition screening program(NSP).

상업스포츠시설 이용자의 스포츠 소비행동에 관한 연구

안영필,정종훈,조재기,김종백 東亞大學校 附設 스포츠科學硏究所 1995 스포츠科學硏究論文集 Vol.13 No.-

With 509 regular members of commercial sports centers as the subjects, such variables as their consumption behavior, expectation & satisfaction were analyzed. For the purpose of setting up a strategic theory of marketing segmentation, this sports market's consumer characteristics and responses were segmented. The following sums up the conclusion : THE Segmentation Matrix of the Commercial Sports Center Market -------------------------------------------------------------------------------- Division Large Users (27.0%) Small Users (24.2%) -------------------------------------------------------------------------------- Class by skill(beginning, Expectation intermediate, advanced, sex, age) Parking lot. Shuttle buses Friendship matches with some invited athletes. ------------------------------------------------------------------------------- Satisfaction Convenient facilities and Price of using the center VIP or discount tickets. -------------------------------------------------------------------------------- Unsatisfactory in convenient Unsatisfactory in divers facilities, clean weter, programs,health counselling, periods and contens of leaders' advise and programs, technicques, class of by skill, number of price of using,shuttle buses, members a class,various and general environment. Satisfaction programs,health counselling, A little satisfactory in the Against leaders' advise,coaching items of parking lot and Expectation echnicques,price of using, special events for shuttle buses,parking lot, member's friendship and environment. Rather satisfactory in the items of convenient membership and attitude to customers. --------------------------------------------------------------------------------

馬山灣에서의 燐과 窒素의 動態에 관하여

안태석,안태영,권오섭,조기성 江原大學校 附設 環境硏究所 1989 環境硏究 Vol.6 No.-

1978년 부터 1982년 까지 마산만 4개 정점에서 인산에 인과 질소 상태에 대한 조사 결과, 인산태 인과 질소 상태에 대한 조사 결과, 인산태 인은 각 정점별 평균치가 0.86∼2.70 μg at - P/ℓ이였고, Ammonia + amino 산태 질소는 1.21 ∼4.36 μg at - L/ℓ이였고, 질산태 질소는 0.94 ∼ μg at - N/ℓ 로 비교적 높은 값을 보였다. 마산시에 인접한 정점 1, 2에서는 질소의 농도가 점차로 증가하고 있는 경향을 보였다. 또한 질소와 인의 비율은 정점 1과 2에서 40을 넘는 값을 보여주어, 질소의 유입이 큰 것을 뜻하므로 이에대한 방지대책이 시급히 요구된다. The concentrations of phosphate-P, ammonia + amino acid-N, nitrite-N, and nitrate-N were measured at 4 sites in the polluted Masan Bay from 1978 to 1982. The mean concentration of phosphate-P, ammonia + amino acid-N, nitrine-N and nitrate-N in each site varied 0.68-1.90 ug at P/1, 1.21-4.36 ug at-N/1, 0.36-3.23 ug at-N/1 and 2.17-29.45 ug at-N/1, respectively. At site 1 and 2, nearyby Masan City, the concentration of nitrogen was gradually increasing according to the increase of the population of the city and the N/P ratios at site 1 and 2 reached more than 40.

조혈모세포이식 환자에서의 기계 환기의 위험 인자 : Assessment of Risk Factors

안중경,이홍기,황정혜,박세훈,이효락,송서영,이순일,박준오,김기현,김원석,정철원,임영혁,강원기,박근칠 대한조혈모세포이식학회 2002 대한조혈모세포이식학회지 Vol.7 No.1

연구배경: 조혈모세포이식을 시행한 환자에서 집중 치료 시 예후가 매우 나쁜 것으로 알려져 있고, 기계 환기는 강력한 사망 예측 인자로서 보고되고 있다. 그러나 현재까지 이식 환자에서 기계 환기의 위험 인자를 밝힌 연구는 매우 드물다. 따라서 기계 환기를 시행한 조혈모세포이식 환자의 임상적 특징을 살펴보고 기계 환기에 대한 위험 인자를 알아보고자 하였다. 방법: 삼성서울병원에서 조혈모세포이식을 시행한 환자 중 기계 환기를 시행한 23명과 기계 환기를 시행하지 않은 142명을 대상으로 후향적 연구를 시행하였다. 기계 환기에 대한 위험 인자의 여부에 관해서는 chi-square 또는 Fisher's exact 검정을 시행하였으며 기계 환기에 대한 각 위험 인자의 영향에 관해서는 다중로지스틱 회귀분석을 시행하였다. 결과: 기계 환기를 시행한 23명의 환자 중 30일째 생존자는 1명이었고 6개월째 생존율은 0%였다. 생존자와 사망자 모두에서 다기관 기능부전이 관찰되었으며, APACHE II 점수와 SAPS II 점수에 의한 예측 사망률은 각각 56%, 59%였다. 조혈모세포이식 후 기계 환기의 위험 인자로는 선행 질환이 혈액질환, 부분불일치 동종이식, 간정맥폐색성질환, 이식 전 질병 상태가 재발하였거나 약물에 반응하지 않는 경우였다. 다중로지스틱 회귀분석 결과 기계 환기의 위험 인자로 통계적으로 유의한 차이를 나타낸 것은 단지 부분불일치 동종이식을 시행한 경우였다. 결론: 현재까지 조혈모세포이식 환자에서 집중 치료의 역할에 대해서는 확실히 정립되어 있지는 않으며, 이식 환자에서 기계 환기는 강력한 사망 예측 인자이다. 따라서, 조혈모세포이식을 시행한 환자에서 기계 환기의 위험 인자와 불량한 예후 인자를 고려하여 기계 환기 여부에 대한 신중한 결정을 내려야 한다. Background: Respiratory failure requiring mechanical ventilation is a frequent, critical complication of hematopoietic stem cell transplantation (HSCT). Patients who require mechanical ventilatory support after HSCT generally have a very poor prognosis. Mechanical ventilation in HSCT recipients is a strong predictive factor of mortality. The objectives of this study are to describe clinical characteristics of HSCT recipients undergoing mechanical ventilation and to identify the risk factors for mechanical ventilatory support after HSCT. Methods: We performed a retrospective chart review of all patients >15 yrs old who received HSCT at Samsung Medical Center and subsequently required mechanical ventilatory support between 1996 and 2001. Results: Thirty-day mortality rate in HSCT recipients undergoing mechanical ventilation was 95.6%. The mean predictive mortality rates of APAHCE II score and SAPS II score were 56% and 59%, respectively. Reasons for mechanical ventila-tion were sepsis (47.8%) followed by fungal infection (13%) and diffuse alveolar hemorrhage (8.7%). Univariate analysis identified relapsed or refractory diseases at HSCT, hematologic disease, hepatic venoocclusive disease and allogeneic or HLA-mismatched transplant as significant risk factors for mechanical ventilation. On multivariate logistic regression analysis, only allogeneic mismatched transplant remained significant. Conclusion: Overall outcome of HSCT recipients undergoing mechanical ventilation is very poor. Therefore, the risk factors and the poor prognostic factors for mechanical ventilation should be taken into account in making further treatment decision for HSCT recipients requiring mechanical ventilation.

부분 치수절단술을 이용한 복잡 치관파절의 치료 : 증례보고

안병덕,김영재,장기택,이상훈,김종철,한세현,김정욱 大韓小兒齒科學會 2008 大韓小兒齒科學會誌 Vol.35 No.2

혼합 치열기 및 초기 영구치열기의 소아,청소년에서 외상에 의한 치관 파절은 빈번하게 발생한다. 치관파절은 치수의 노출 유무에 따라 단순 치관파절,복잡 치관파절로 분류된다. 치수가 노출된 복잡 치관파절의 경우 치수에 대한 고려 및 처치가 필수적인데,초기 영구치열기에는 전치부 치근의 발육이 완전히 이루어지지 않았기 때문에 완성된 치근을 가지는 치아보다 치료가 복잡하다. 치료의 목적은 적절한 치수 치료를 통해 치근의 완성을 도모하는 것으로 이를 위해 직접 치수복조술,부분 치수절단술,치경부 치수절단술의 방법을 이용한다. 이 중 부분 치수절단술은 성공률도 높으며 치경부 치수를 보존하기 때문에 여러 장점을 지니는 치료방법이다. 복잡 치관파절을 주소로 내원한 환자에서 부분 치수절단술을 시행하여 양호한 결과를 나타내어 이를 보고하는 바이다. Traumatic crown fracture in mixed dentition or early permanent dentition is relatively common. Crown fracture is classified into simple or complicated crown fracture by the presence of pulp exposure. The condition of pulp must be considered in treatment of crown fractures with pulp exposure. Treatment of immature crown-fractured incisor with pulp exposure is more complex because of its incomplete root formation. Pulp capping, partial pulpotomy, cervical pulpotomy can be used for continuous root development in immature crown-fractured incisor with pulp exposure. The success rate of partial pulpotomy is very high and there are several advantages of partial pulpotomy because the cell-rich coronal pulp tissue is preserved. This paper reports 2 cases of crown-fractured permanent incisors with pulp exposure that had been treated by partial pulpotomy successfully.