Focal adhesion kinase and src expression in premalignant and malignant skin lesions

( Ju Yeon Choi ),( Jae Yun Lim ),( Han Saem Kim ),( Jung Min ),( Jung In Kim ),( Hyun Min Seo ),( Sang Hyeon Hwang ),( Chong Won Choi ),( Yoon Hwan Kim ),( Jin Hee Sohn ),( Hyunjoo Lee ),( Won Serk Ki 대한피부과학회 2015 대한피부과학회 학술발표대회집 Vol.67 No.2

Background: Focal adhesion kinase (FAK) and Src are non-receptor tyrosine kinases. FAK and Src play a critical role in inducing malignant transformation in tumor cells. Objectives: We performed immunohistochemical staining for total and phosphorylated forms of FAK and Src, to evaluate the role of FAK and Src in the development of premalignant and malignant skin lesions. Methods: A total of 59 facial skin samples (30 actinic keratoses, 10 Bowen``s diseases, 13 squamous cell carcinomas and six perilesional skins) were immunohistochemically stained for Ki-67, total (t) and phosphorylated (p) form of FAK and Src. Methods: A total of 59 facial skin samples (30 actinic keratoses, 10 Bowen``s diseases, 13 squamous cell carcinomas and six perilesional skins) were immunohistochemically stained for Ki-67, total (t) and phosphorylated (p) form of FAK and Src. Results: Cells positive for t-Src, p-Src-y530, t-FAK and pFAK-s722 were detected in premalignant intra-epithelial lesions (PELs) and squamous cell carcinomas (SCCs), but not in the perilesional skin. There was a tendency towards high correlation between Ki-67 and t-FAK or pFAK-s722, suggestive of the active role of FAK in cell proliferation. Conclusion: Our findings of higher t-Src and p-Src-y530 positive cells in PELs, as compared to SCCs (with higher Ki-67 level), are suggestive of the other role of Src in tumor formation and progression, which requires further investigation.

소멸된 고환암의 혈행성 폐전이 1예

서기현 순천향의학연구소 2004 Journal of Soonchunhyang Medical Science Vol.10 No.2

The tern 'regressed or burned-out tumor' of the testis describes a spontaneously involuted testicular tumor which presents by its metastases to retroperitoneum, mediastinum, lymph nodes such as supraclavicular, cervical and axillary, lungs, and liver. This is a rare form of testicular germ cell neoplasia. Author presents the findings on scrotal sonography, contrast-enhanced chest CT, and abdominopelvic CT of a testicular regressed tumor in a 29-year-old man presenting with hematogenous lung metastasis.

항암 치료에 잘 반응하는 악성 중피종 1예

서기현 순천향의학연구소 2004 Journal of Soonchunhyang Medical Science Vol.10 No.2

Despite many years of clinical research, there is still no effective therapy for malignant pleural mesothelioma (MPM). Untreated, the prognosis is poor, with a median survival of < 1 year. In general, MPM is a disease confined to the pleural cavity for a long time before metastasizing. Therefore, surgical resection has been considered the mainstay of treatment by some. However, surgery alone results in high recurrence rates, and the survival benefit remains questionable. In recent years. the gemcitabin/cisplatin combination is a valid option in the treatment of MPM. In this case, a patient had histologically proven MPM with no previous surgery, chemo- and/or radiotherapy. He underwent the gemcitabin/cisplatin combination chemotherapy. Thereafter he has been free of disease and symptoms for 6 months. I presents a case of malignant mesothelioma showing marked improvement after gemcitabin/cisplatin chemotherapy.

편도체 기저외측핵의 AP5 투여가 상승된 경악반응의 습득에 미치는 영향

서미숙,조소현,김기석,이만영 한국심리학회 한국심리학회지 생물 및 생리 Vol.5 No.1

본 연구는 고전적 공포조건화에 관여하는 장소로 알려진 편도체의 기저 외측핵에 장기상승작용(Long-Term Potentiation: LTP)의 생성을 차단하는 약물인 MNDA (N-methyl-D-aspartate)길항제 AP5를 미세주입함으로써 시각적 조건자극(CS)을 사용한 경우에, 상승된 경악 반응의 습득이 차단되는지를 보고자 하였다. 동물은 AP5-AP5, AP5-식염수, 식염수-AP5, 식염수-식염수 집단으로 나누어서 CS-US 배쌍직전에 AP5나 식염수를 주입한 후 훈련시켰다고 다시 검사직전에 AP5 혹은 식염수를 주입하였다. AP5-AP5집단과 AP5-식염수집단은 상승된 경악반응을 보이지 않았고, 식염수-AP5, 식염수-식염수 집단은 상승된 경악반응을 보였다. CS-US배쌍 직전에 주입한 AP5는 상승된 경악반응을 차단하였고, 검사직전에 주입한 AP5는 상승된 경악반응을 차단하지 않은 결과는 AP5가 습득을 차단했으되 그 표현에는 영향을 미치지 않았다고 해석된다. 그리고 AP5-AP5, AP5-식염수 집단이 상승률에서 유의미한 차이를 보이지 않았으므로, 상승된 경악반응의 차단이 상태의존적 인출 실패에 기인한 것이 아니고, AP5 자체의 약효때문임을 알 수 있다. It has been known that the amygadala is the neural substrate for conditioned fear as well as unconditioned fear. Among the substructures of amygdala, the basolateral nucleus where CS and US inputs converge and LTP occurs, contains high densities of NMDA receptors and so seems to have a critical role in synaptiplasticity. NMDA antagonist, AP5 prevent induction of Long-term potentiation, but not expression of LTP. LTP is an activity dependent enhancement of synaptic efficacy and is regarded the psysiological mechanisms that might underlie learning and memory. So this experiment was done to investigate what effect AP5 injection to the basolateral amygdala on the acquisition and expression of the fear conditioning, using the fear potentiated Startle paradigm. Animals were allocated to AP5-AP5, AP5-saline, saline-AP5, saline-saline groups. AP5 or saline was injected just before conditioning and testing. The re sult is that AP5-AP5, AP5-saline group didn't show the potentiated startle, comparative to the saline-AP5, saline-saline group, and AP5-AP5 group is not significantly different from AP5-saline. So we conclude that AP5 blocked the acquisition but not expression of conditio-ned fear-potentiated startle and convince that the blocking is not due to state-dependent retrieval failure.

Nuss 방법에 의한 소아 오목가슴 수술 후 정맥 통증자가조절법을 사용한 Fentanyl 과 Fentanyl-Ketorolac의 진통효과 비교

안기량,서은정,김천숙,강규식,유시현,정진헌,정지원 순천향의학연구소 2006 Journal of Soonchunhyang Medical Science Vol.12 No.2

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향토수목원 및 관광지 조경을 위한 자생식물의 개발과 이용

윤영활,서옥하,이기의,조현길 江原大學校 森林科學硏究所 1998 Journal of Forest Science Vol.14 No.-

要約우리는 우리 지방에 자생하면서 기후풍토에 적응된 아름답고 진귀한 많은 화목류와 교목류 등을 부존하고 있으면서도 이들을 연구 개발하지 않고 주로 도입종과 재배종에만 의 존하고 있는 것이 오늘의 현실이다. 그러므로, 국내외의 광광객이 dyurn하는 즉, 강원도는 물론 우리나라 고유의 정취와 이미지를 줄 수 있는 많은 야생종들을 연구, 개발하여 조경식 물화시켜 대량으로 번식시키는 동시에 일반 재배화함으로써 향토색 짙은 관광한국을 만들어 관광사업의 근본목표인 경재적 효용을 다하기 위해 매우 개발가치가 있는 교복류 37종과 관 목류 73종의 특성, 재배법, 용도 등을 조사하였다.ABSTRACTThis study examined ecophysiological characteristics, cultivation methods and planting uses of tree and shrub species native to Kangwon province and valuable as landscape plants.The results were as followos :1. 73 species selected as shrubs were; Rhododendron brachycarpum, Rhododendron micranthum, Abelia mosanensis, Abeliophyllum distichum, Acanthopanax sessiliflorus, acanthopanax senticosus, Alangium platanifolium var. macrophyllum etc. 2. 38 species selected as trees were ; Cornus controversa, Sorbiis commixta. Mains baccata, Prunus padns, Acer triflorum, Acer tegmentosum, Styrax japonica, Styrax obassia, Sorbiis ainifolia etc. 3. Shade-tolerant species were ; Abies koreana. Rhododendron brach\carpum. Rhododendron micranthum, Primus padus, Sorbus commixta, Acer triflorum, Rhus japonica, Cornus kousa, Kalopanax pictus, Chionanthus retusa, Sorbus ainifolia, Magnolia sieboldii, Sorbaria sorbifolia var. stellipila, Berberis koreana, Lindera obtiisiloba, Rhododendron schlippenbachii and Euonymus alatiis. 4. Species growing well even in less fertile soils were ; Finns thunbergii, Quercus dentata, Koelreuteriapaniculata, Rhus japonica, Quercus acutissima, Albiz^ia julibrissin, Sorbus ainifolia, Staphylea buinalda, Philadelphus schrenckii, Clerodendron Iriclwtomum, Deut^ia coreana, Weigela florida, Zanthoxyiuin schinifolium and Lindera obtusiloba. 5. Salt-tolerant species were ; Finns thunbergii, Quercus aliena, Primus padus, Styrax japonica, Quercus dentata, Kalopanax pictus, Chionanthus retusa, Albizua julibrissin, Tilia amurensis, Forsvthia koreana, Lonicera inaackii, Samplocos chinensis for. pilosa, Clerodendron trichotomum, Sambiicus williamsii var. coreana, Abelia mosanensis, Deutua coreana. Viburnum cariesii, Rhus japonica, Rhododendron yedoense var. poukhanense, Zanthoxylum schinifolium, Callicarpa dichotoma, Rhododendron schlippenbachii and Euonymusalatus. 6. Pollutant-tolerant species were ; Styrax japonica, Prunus padus, Quercus dentata, Kalopanax pictus, Chionanthus retusa, Gleditsia japonica var. koraiensis, Styrax obassia, Forsythia koreana, Spiraea salicifolia, Clerodendron trichotomum. Viburnum sargentii, Viburnum cariesii, Weigela florida and Rhododendron vedoense var. poukhanense.

비만을 동반한 위암 환자에서 두 병을 같이 수술 치료한 경험(증례)

최경현,윤기영,문형환,신연명,서경원,안수미,송윤미,석정희,정경연,이은하 고신대학교의과대학 2008 고신대학교 의과대학 학술지 Vol.23 No.3

Obesity is growing problem in Korea. We had a case of bariatric surgery during gastric cancer operation. Patient was 29 year old Korean lady with early gastric cancer located in the lesser curvature side of the middle 1/3 of the stomach. Her body weight was 89 kg, height 163 cm, and thus body mass index was 33.5 kg/m2. Preoperative blood pressure was 130/90 mmHg, hemoglobin 12.9 g/dL, total lymphocyte count 3,280/mL, serum albumin 4.3 g/dL, CEA 1.1 ng/mL, CA 19-9 9.1 ng/mL, and alpha fetoprotein 2 ng/mL. Another associated disease was right thyroid follicular neoplasm. The chest X-ray was normal. After IM injection of 2,500 units of heparin 30 minutes before the induction of general anesthesia, she received curative subtotal gastrectomy and Roux en Y gastrojejunostomy when a malabsorption loop of 120 cm jejunum was incorporated between the Treitz ligament and the end to side jejuno-jejunostmy site at May 29th 2006. Her postoperative course was uneventful except a minor wound seroma and the elevations of serum amylase(up to 4 folds) and lipase(up to 2 folds). She lost her body weight 9 kg in 5th, 21 kg in 7th, and 27 kg in 10th postoperative months to became 61.5 kg. On follow up exams in July 2008, she gained 4 kg to overcome her weakness and fatigue. From the above result, the incorporation of a malabsorption loop during reconstruction phase of gastric cancer operation was a good option for obese gastric cancer patients especially in cases of early gastric cancer.

실시간 엘리베이터 감시 및 제어 시스템의 설계 방법론

서현곤,김기형 嶺南大學校 工業技術硏究所 1998 工業技術硏究所論文集 Vol.26 No.2

As modern buildings become larger and higher, the number of elevators to be controlled in buildings also increases. For efficient management of the elevators in a building, a real-time elevator status monitor and control system is essential. In this paper, we present a design methodology of the real time elevator monitor and control(EMC) system. The EMC system is designed to be connected directly to the internal network of elevator systems, thereby efficient monitoring and control can be possible.

뇌동맥류의 진단에 대한 전산화단층촬영 뇌혈관조영술과 고식적 뇌혈관조영술의 비교

송상현,윤수한,안영환,안영민,조기홍,조경기,김선용,서정호 대한신경외과학회 1996 Journal of Korean neurosurgical society Vol.25 No.10

Cerebral angiography has been essential for the diagnosis of the intracranial aneurysms but. is sometimes accompanied by serious complications. Resolution of CT angiography was up-graded greatly to represent the three-demensional structure of vesseles since helical CT had been introduced. We have compared 26 cases of CT angiography and 28 cases of conventional angiography in terms of specificity and sensitivity for the diagnosis detectable aneurysm diameter. configurational diagnosis and diagnostic confidency. All results showed no statistical difference between CT angiography and conventional angiography. These should suggest that CT angiography could be replaced with conventional angiography for the diagnosis of ruptured aneurysms and even of unruptured aneurysms. resulting in the introduction of first screening modality of unruptured aneurysms.

저혈청 배지에서 인간 전골수세포(HL-60)를 이용한 tPA 생산과 세포사멸기작에 관한 연구

김현구,성기돈,김태호,안주희,함문선,박진서,이현용 한국미생물생명공학회 ( 구 한국산업미생물학회 ) 1996 한국미생물·생명공학회지 Vol.24 No.1

tPA 생산과 세포 사멸의 연구를 하기 위해서 인간 전골수세포가 배양되었다. 연속 관류 배양하에서 최대 tPA 생산량은 324 ng/ml이며 최대 세포 농도는 5.27×10 exp (6) cells/ml였다. Phorbol ester를 넣었을 때 tPA 생산량은 420 ng/ml까지 향상되었다. 형광 현미경으로 관찰했을 때, apoptosis는 세포 배양 초기부터 중반까지 우세했고 빠른 교반 속도에서도 우세했다. 그러나 necrosis는 세포배양 말기와 동결된 세포가 해동 되었을 때 우세했다. HL-60 was cultivated to produce tPA (tissue-type plasminogen activator) and study the mechanism of cell death. Maximum cell density and tPA production were obtained as 5.27×10 exp (6) cells/ml and 324 ng/ml, respectively under perfusion cultivation. tPA production was enhanced to 420 ng/ml in adding 160 nM of phorbol ester. The cells were gradually differentiated to granulocytes rather than proliferation. By Fluorescent microscope, apoptosis was prevailed except the death phase and in high agitation speed, but necrosis was prevailed in thawed cells and during the latter periods of the cultivation. It was also proved that tPA was most produced in apoptosis. To obtain higher tPA productivity, the cells must be maintained in apoptosis, not necrosis phase when the cells were dying.