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건조 담체 고정화 Zymomonas mobilis 세포의 oxidoreductase 활성도
장기효,이제혁,전억한 경희대학교 유전공학연구소 1991 遺傳工學論文集 Vol.3 No.-
박테리아인 Zymomonas mobilis는 glucose와 fructose를 각각 gluconic acid와 sorbitol으로 동시전환할 수 있다. Glucose fructose oxidoreductase에 의하여 glucose의 산화와 fructose의 환원이 일어난다. 균체를 cationic detergent인 CATB(cethyltrimethylammoniumbromide)로 처리하여서 투과성을 높인 후에 glutaraldehyde로 crosslinking 하였던 결과, 균체에서의 glucose-fructose oxidoreduc-tase의 유출이 감소되었으며 고정화균체의 안정성이 증가하였다. 투과성을 증가시킨 균체를 K-carra-geenan으로 고정화하여서 연속공정을 실시한 결과 효소의 안정성이 30일 이상 지속되었다. Kcarrageenan으로 고정화한 균체를 건조시 beads의 견고성과 저장성의 향상이 나타났다. 건조된 Kcarrageenan 담체의 V_(max)값은 39C와 pH6.2에서 free cells의 거의 절반값을 나타내었다. 연속공정에서 회석비율 0.1h^(-1)일때 wet beads와 건조 beads에서의 sorbitol 생산성은 각각 3.2∼3.5, 2.85∼2.9(g/l-h)를 나타내었다. The bacterium Zymumonas mobilis is able to convert glucose and fructose to gluconic acid and sorbitol simultaneously. The enzyme complex, which is responsible for glucose oxidation and fructose reduction, has been described as glucose-fructose oxidoreductase. After treatment of the cells with cationic detergent CTAB(Cetyltrimethylammoniumbromide) for permeabilization, the cells were treated with glutaraldehyde in order to crosslink enzymes. Glutaraldehyde treatment of the permeabilized cells could improve the stability of the system by decreasing the loss of glucose-fructose oxidoreductase. The methods of glutaraldehyde cross-linking of enzymes in CTAB treated cells immobilized in K-carrageenan showed a stable ability of enzymes in CTAB treated cells oxidoreductase for 30 days of process. K-carrageenan beads entrapped with permeabilized cells were dried to improve bead rigidity and storage stability. The value of V_(max) for the dry K-carrageenan beads was found to a 1/2 of that of free cells. It was shown that the productivities of the continuous process with wet K-carrageenan beads and dry beads at dilution rate 0.1h^(-1) were 3.2-3.5 g/l-h and 2.85-2.9 g/l-h, respectively.
구순열 및 구개열 환자에 대한 Toxoplasma항체의 혈청학적 연구
심영기,이세일,홍인표,정평림,장효죽 大韓成形外科學會 1993 Archives of Plastic Surgery Vol.20 No.1
The etiology of the cleft lip and palate can be explained with a multifarious theory. According to articles, there are some relationships between congenital toxoplasmosis and the cleft lip&palate, but the direct relationships is not obvious. Therefore, this research aims to find the difference of the serum toxoplasma antibody titer between 172 cleft patients who have been treated at National Medical Center and their mother group of 81 cases, healthy control group of 150 cases. The antibody titer was identified by the indirect fluorescent antibody test(>16)and the enzyme-linked immunosorbent assay(>0.3). The results are as follow : 1. By ELISA, the positive rate of the cleft patients(8.1%, 14cases)and that of the mother group(9.9%, 8cases)is higher than the healthy control group(2.7%, 4cases)(p<0.05). 2. By IFAT, the positive rate of the cleft patients(10.5%, 18cases)is higher than the healthy control group(5.3%, 8cases), but the difference is not significant(p>0.05). And the positive rate of the mother group(13.6%, 11cases)is higher than the healthy control group(5.3%, 8cases)(p<0.05). 3. By ELISA and IFAT, the positive rate of the mother group is higher than the cleft patient but the difference is not significant. 4. By ELISA and IFAT, the positive rate of the cleft patients according to the clincal group is not significant. 5. By ELISA and IFAT, the positive rate of the chronic disease group(17.3% & 27.7%)is higher than the healthy control group(2.7% & 5.3%)(p<0.05).
Duplication or deletion of chromosome 17p11.2-p12 in CMT1 and HNPP peripheral neuropathy patients
Kim, Hyo-Sun,Jang, Young-Eun,Chung, Ki-Wha 公州大學校 基礎科學硏究所 2003 自然科學硏究 Vol.10 No.-
CMT와 HNPP 말초신경증은 가장 흔하게 발병하는 운동 및 감각 말초신경계의 선천성 질환이다. CMT1과 HNPP의 유전적 원인으로는 염색체 17p11.2-p12의 1.5 Mb 지역의 중복과 결실이 각각 50%이상과 70% 이상을 차지하는 것으로 알려졌다. 본 연구에서 한국인 CMT1 및 HNPP 환자를 대상으로 중복 및 결실을 조사하였는데, 31 CMT1 가족 중 13 가족에서 중복을 보였으며 (42%), 24 HNPP 가족 중 19 가족에서 결실을 보였다 (79%). 17p11.2-p12 중복을 보이는 CMT1A 환자가 HNPP 결실환자보다 발병 연령, 발병 기간, 근육 이양성 및 족 기형성에서 훨씬 심한 증상을 보였다. Charcot-Marie-Tooth disease (CMT) and hereditary neuropathy with liability to pressure palsies (HNPP) are most common inherited motor and sensory peripheral neuropathies. The majority of CMT1 patients have a 1.5 Mb duplication in chromosome 17p11.2 while most HNPP patients have a deletion of the same region. In the analysis of Korean CMT1 and HNPP patients, each HNPP deletion and CMT1A duplication was observed from 79% (19 families) among 24 HNPP families and 42% (13 families) among 31 CMT1 families. CMT1A duplication patients showed more severe clinical symptoms than HNPP deletion patients, when we examined oneset age, disease duration, muscular atrophy, foot deformity and scoliosis.
High Stiffness를 갖는 Digital 직류 Servosystem 설계
홍창희,장기효,장윤영 동아대학교 공과대학 부설 한국자원개발연구소 1989 硏究報告 Vol.13 No.2
The conventional proportional P controller has been used as the position controller of the dc servomotor. When the unknown and inaccessible load torque, such as the coulomb friction and so on, is imposed on the dc servomotor, this controller system has the stedy state error and transient-state error. this will be reduced by a new method in this paper, which is composed P type controller is based on the observer and programmed in the computer. by this method, We can obtain high stiffness of the DC servomotor control system. The discrete observer should be employed because this type of observer can estimate the external force simply and fast.
임영환,양시은,장동영,안효석,홍석기 한국공작기계학회 2008 한국공작기계학회 춘계학술대회논문집 Vol.2008 No.-
본 논문에서는 레이저 빔 투과를 이용한 본딩 웨이퍼 검사 방법을 제안하고 검사 장치를 설계 구현하였다. 1064nm파장에서의 정상웨이퍼를 일정한 비율로 투과하였다. 본딩 불량으로 인한 웨이퍼의 기공은 두께에 따라 투과율이 현저하게 변화하여 기공 부분을 검출하였다. 이러한 기공은 두께의 변화가 있으며 광량의 변화하는 부분이 에어갭으로 인식 카메라로 쉽게 구분이 가능하였다.
간경변증 환자에서 Escherichia coli 균혈증 합병 시 간기능 장애에 따른 C-reactive protein 생성 능력에의 영향
박완범,강철인,김동민,이기덕,장희창,김홍빈,오명돈,이효석,최강원 대한감염학회 2003 감염과 화학요법 Vol.35 No.5
목적 : C-reactive protein(CRP)은 간에서 생성되는 급성 반응물질이다. 하지만 간부전증 환자에서 CRP의 반응이 간기능에 따라 어느 정도 영향을 받는지는 별로 알려진 바가 없다. 본 연구에서는 간기능에 따른 CRP 생성 능력을 평가하고자 하였다. 방법 : E. coli 균혈증이 있는 간경변증 환자 30명을 대상으로 하였고 간기능은 균혈증이 발생하기 전 2개월 이내에 측정된 혈청 빌리루빈, 혈청 알부민, 프로트롬빈시간, Child-Pugh 점수로 평가하였다. 대조군 A는 간질환이 없으면서 E. coli 균혈증이 발생한 환자 30명으로 하였고 대조군 B는 간경변증이 있으면서 급성 감염의 증거가 없는 환자 30명으로 하였다. 환자군과 대조군 간에 CRP의 최대값을 비교하였다. 결과 : CRP의 최대값은 환자군에서 7.3±5.0㎎/dL, 대조군 A에서 17.9±8.3㎎/dL로 환자군에서 유의하게 낮았다.(P<0.001). 간경변증 환자에서 CRP의 생성은 Child-Pugh 점수에 비례하여 감소하였으나(P=0.004) Child=Pugh class C의 간기능을 가진 환자군에서도 대조군 B와 비교하여 의미있는 CRP의 생성을 보였다(5.3±3.2 vs. 0.5±0.4㎎/dL, P<0.001). 결론 : 간기능부전 환자에서 CRP 반응은 간기능 저하 정도에 따라 둔화되지만 심한 간기능 장애를 가진 환자에서도 CRP의 생성은 유지된다. Background : C-reactive protein (CRP) is an acute phase reactant produced in the liver. To assess the influence of liver dysfunction on the production of CRP, we evaluated CRP response to E. coli bacteremia in patients with or without liver cirrhosis (LC). Methods : 30 LC patients who developed spontaneous peritonitis with E. coli bacteremia were enrolled in the study. Baseline values of total bilirubin, serum albumin, and prothrombin time were obtained within 2 months prior to infection. Liver dysfunction was categorized according to the Child-Pugh score. 30 patients with E. coli bacteremia who had no underlying liver dysfunction were included as a control group. Matched-control of 30 LC patients without evidence of acute infection was also included. The peak CRP values were compared among the groups. Results : In the patients with E. coli bacteremia, the mean value of peak CRP was 7.3 (+/- 5.0) ㎎/dL in LC patients, 17.9 (+/- 8.3) mg/dL in patients without liver dysfunction (p<0.001). In the advanced LC patients with Child-Pugh class C, the level of CRP was 5.2 (+/- 3.3) ㎎/dL in patients with E. coli bacteremia, 0.5 (+/- 0.4) ㎎/dL in patients without acute infection (P<0.001). Child-Pugh score had correlation with decrease of CRP (linear regression test, P=0.004). Conclusion : CRP response during E. coli bacteremia was attenuated but maintained even in patients with advanced liver dysfunction.