韓國에서 發生되는 벼 오갈病에 關한 硏究

李起運 慶北大學校 1984 論文集 Vol.38 No.-

This experiment was conducted to obtain basic informations for an integrated control of the disease by studying the occurrence, damage disease cycle comprising virusinsect vector-plant, host range, and, testing methods for varietal resistance. The results obtained are as follow. It is expected that the disease be limited to southern area below the line connecting Daejon, Boeun and Uljin in Korea because viruliferous vectors are not able to overwinter in the area above the line. As a result, rice dwarf does not occur in seedling bed in the northern area although the disease may be found in the paddy field by infection by viruliferous vectors moved from southern area. High yield strains have been planted extensively for their high yield. In southern area ratoons of the high yield strains remain alive 8-30 days after harvest. The ratoons of infected plants serve insect vectors for acquisition of the virus by feeding and contribute to the increased number of the overwintered viruliferous insect vectors. Foot path serves as a habitat for insect vectors and contribute to higher disease occurrence near the foot path than in the middle of the field. When rice plants were inoculated at different leaf stages with the virus, high yield strains showed less infection than japonica strains in vegetative growth stage, but difference between the two strains was minor inreproductive growth stage. Incubation period in the plant was also longer in high yield strains than in Japonica strains. Inoculation of japonica strain rice at 3-9 leaf stage resulted in a high percentage of withering, whereas high yield strains inoculated at 5 leaf stage or later showed relatively low percentage of withering. Heading was possible only when rice plants of both high yield and japonica strains were inoculated at 7 to 9 leaf stages or later and ripening only at 9 leaf stage or later. Thirty five species of plants including cereals and weeds were tested for host of the rice dwarf virus. Twenty nine of them were identified to be hosts of the virus. There were Oryza sativa L. Nagdongbyeo, Hordeum sativum Jess. Olbori, Triticum aestivum L. Dahong mil, Avena sativa L. Zea mays L. Suwoen╋29, Setaria italica Beauv, Alopecurus aequalis Sosbol, Echinochloa hispidula Nakai, Echinochloa echinata Nakai, Glyceria acutiflora, Panicum miliaceum, Phleum pratense L., Poa annua L., Echinochloa crusgalli var. frumentosa, Secal cereal L. Digitaria sanguinalis L. var. cilialis, Digitaria sanguinalis Scopoli var. aultinervis Honda, Eleusine indica Gaert., Zoysis japonica Steudel, Arthraxon hispidus (Thunberg), Leersia sayanuka, Setaria viridis Beauvis var. purpurascens, Eragrostis curvular Mees, Eleocharis acicularis L., Eriocaulon atrvm Nakai, Eriocaulon robustius Nees., Elymus arenarius L. Coix agrestis Lour, Isachne globosa Kuniz. When the rice dwarf virus was double inoculated with other viruses such as rice stripe virus and rice black-streaked dwarf virus, only 6.7 to 17.0% of double infection was obtained. But the rate was relatively low compared to single inoculation indicating that infection is inhibited by double inoculation. Only 0.5 to 1.6% of barley seedlings infected with rice dwarf virus servived winter. This suggests that overwintering of the virus on infected plants is almost impossible. In fluctuation in occurrence of vectors, peak period was late July. Percentage of infected hills rapidly increase early in July and late in August. This suggests that overwintered adults, nymphs of second generation, and nymphs of third generation served as transmission of the virus. The percentage of viruliferous vectors was the highest in the second generation ones. Insect vectors can acquire infectivity by feeding infected plants only for 48 hours. Incubation period in the insect body was about 10 to 18 days. Vectors could be infective by injection with juice of infected plants or viruliferous insects. The transmission activity was high in nymphal stage and virus affinity was relatively highl in ecotype Ⅱ-a vectors collected from southern part of the peninsula. Inoculating seedlings at 3 leaf stage with an inoculum level of 1 to 2 insects was effective. On the physiopathological ecology of viruliferous vectors, viral infection reduced oviposition period of female vectors, and the number of hatched nymphs from oviposited eggs. Especially infected insects were sensitive to alternating temperature with low Causal virus of the rice dwarf disease was purified by an improved method. Purity was 3.24㎎/ml. Purified virus was injected to rabbit as an antigen and an antiserum with a titer of 1/4096 was obtained. When viruliferous vector and infected leaves were used as an antigen, detection of the virus was possible upto 1/8192 dilution by enzymelinked immunosorbent assay(ELISA) test. Seedlings of ninety lines of rice were inoculated for testing resistance. Eight lines including Milyang 30 were resistance, 23 lines including Nampung were moderate resistance and 60 lines including Kaya were susceptible. In mass screening of seedlings, of the 20 lines tested, Milyang 30 was resistance Naekyung and Nampung were moderate resistance and all the rest 17 lines were susceptible. In field screening, of the 64 lines tested, 20 lines including Milyang 30 were resistance, 15 lines including Baekyang were moderate resisance and 29 lines including Kaya were susceptible. temperature.

園藝作物(가지科)의 바이러스病 發生生態에 對하여

李起運,李準琸 COLLEGE OF AGRICULTURE KYUNGPOOK NATIONAL UNIVERSI 1984 慶北大農學誌 Vol.2 No.-

Several ecological factors were investigated for the virus disease occurring on the horticultural crops (Solanaceae spp). The results obtained were as follows: In the survey of primary biological inoculum, 42 weed species showing viral symptoms were collected on the circumference of pepper, potato and tomato fields. The number of weed species showing viral symptoms were ; 38 species on pepper field, 24 species on potato field and 21 species on tomato field, respectively. The screening of viruses revealed that more weeds are infected by Cucumber Mosaic Virus(CMV) and Potato Virus Y(PVY) than by Tobacco Mosaic Virus (TMV) and Potato Virus X (PVX). In single and double infection with viruses such as TMV, CMV and P VY on the pepper fields, the virus infection phases were different according to the area and according to the screening time. The pepper plants cultivated in Youngyang area were much more infected by virus than in Taegu, Namji and Kimchun area where the improved field hygien was applied. The severity of the disease in Youngyang area was mainly due to the double infection from the early growth stage. Transmission activity of TMV in different cultivation method on tomato was distinguishably higher in outdoor cultivation with furrow irrigation than vinyl house cultivation with furrow irrigation and outdoor cultivation without furrow irrigation. No significant difference the transmission activities was found between of vinyl house cultivation with furrow irrigation and outdoor cultivation without furrow irrigation.

慶北地方 果樹線蟲에 關한 硏究

崔永然,李起運 慶北大學校 1980 論文集 Vol.29 No.-

As the results of this research to the plant parasitic nematodes associated with apple trees in Kyungpook Province, Korea, 25 species belonging to 19 genera under 11 families were detected. Among them two species; Hemicycliophora koreana and Merlinius koreanus were already described as new species from apple orchards by the author separately. Seven species are newly recorded from apple trees; Eutylenchus africanus, Helicotylenchus dihystera, Hemicriconemoides varionodus, Merlinius nothus, Neolobocriconema serratum, Pratylenchus penetrans, Xiphinema americanum. Nematode species and density in each surveyed localities are given. The genus Tylenchorhynchus is dominant genus at apple orchards in kyungpook province, and the second is the genus Pratylenchus.

Nucleotide Sequence of Coat Protein Gene of Kyuri Green Mottle Mosaic Virus Isolated from Zucchini

Lee, Su-Heon,Lee, Young-Gyu,Park, Jin-Woo,Park, Hong-Soo,Kim, Yeong-Tae,Cheon, Jeong-Uk,Lee, Key-Woon The Korean Society of Plant Pathology 2000 Plant Pathology Journal Vol.16 No.2

The coat protein (CP) gene of kyuri green mottle mosaic virus zucchini strain (KGMMV-Z) isolated from zucchini (Cucurbita pepo) in Chonfu, Korea in 1999 was sequenced by the reverse transcription and polymerase chain reaction with degenerate and generate primers originated from tobamoviruses. The degenerate primers were very effective in amplification of KGMMV-Z CP region. The KGMMV-Z CP gene consisted of 486 nucleotides and had the same nucleotide length compared with those of cucurbit-infecting tobamoviruses. KGMMV-Z CP gene shared 43.8, 44.2, and 44.4% nucleotide sequence similarity with the CP gene of cucumber green mottle mosaic virus watermelon strain (CGMMZ-W), CGMMV-KW1, and CGMMV-SH, respectively, whereas three CGMMV strains among themselves showed 98.6-99.6% nucleotide similarity. The deduced amino acids of KGMMV-Z CP gene were 161 amino acid residues with the molecular weight of 17,181 daltons. The first 24 codons of KGMMV-Z CP gene corresponded to the sequences of the N-terminal amino acid of the viral capsid protein. The amino acid sequences of KGMMV-Z CP had 45.3% similarity compared with those of three CGMMV strains. However, the amino acid sequences of CGMMV strains were identical. These results showed that two cucurbit-infecting tobamovirus members, KGMMV-Z and CGMMV were genetically distantly related.

99mTc - HMPAO Brain SPECT in a Cryptococcal Meningitis Patient

Lee, Dong Soo,Chung, June Key,Kim, Jong Ho,Lee, Kyung Han,Lee, Myung Chul,Koh, Chang Soon,Choi, Chang Woon,Yang, Hyung In 대한핵의학회 1993 핵의학 분자영상 Vol.27 No.1

Measurement of Local Cerebral Glucose Utilization in the Rat by the Autoradiographic 2 - Deoxy - D - [14C] - Glucose Method

Lee, Dong Soo,Chung, June Key,Kim, Sang Eun,Lee, Myung Chul,Lee, Young Min,Koh, Chang Soon,Choi, Chang Woon,Yoon, Byung Woo,Roh, Jae Kyu,Bae, Sang Kyun,Hong, Seung Bong,Hong, Mee Kyung 대한핵의학회 1992 핵의학 분자영상 Vol.26 No.1

An Automatic Tagging System and Environments for Construction of Korean Text Database

Woon-Jae Lee,Key-Sun Choi,Yun-Ja Lim,Yong-Ju Lee,Oh-Woog Kwon,Hiong-Geun Kim,Young-Chan Park 한국소음진동공학회 1994 한국소음진동공학회 국제학술대회논문집 Vol.1994 No.4

Nucleotide Sequence of Coat Protein Gene of Kyuri Green Mottle Mosaic Virus Isolated from Zucchini

Su Heon Lee,Young Gyu Lee,Jin Woo Park,Hong Soo Choi,Yeong Tae Kim,Jeong Uk Cheon,Key Woon Lee 한국식물병리학회 2000 Plant Pathology Journal Vol.16 No.2

관상동맥질환에서 휴식기의 심근관류 정도와 휴식기 심기능 변화의 비교 - Dipyridamole 부하 99mTc - MIBI SPECT와 휴식기 Gated Blood Pool Scan -

이명철(Myung Chul Lee),정준기(June Key Chung),이영우(Young Woo Lee),서정돈(Jung Don Seo),박영배(Young Bae Park),이동수(Dong Soo Lee),김상은(Sang Eun Kim),최창운(Chang Woon Choi),이명묵(Myoung Mook Lee),고창순(Chang Soon Ko),배상균(Sa 대한핵의학회 1992 핵의학 분자영상 Vol.26 No.2

N/A Rest gated blood pool scan (Rest GBP scan) and dipyridamole Tc-99m-MIBI SPECT were performed in 34 patients with or suspected coronary artery disease. Both studies were performed within 2∼32 days (mean 8.1 days). A significant correlation was present between left ventricular ejection fraction (r=-0.7356, p〈0.001) and peak ejection rate and peak filling rate in rest GBP scan and perfusion defect in MIBI SPECT. And there were acceptable correlations (0.05〈p〈0.001) between regional ejection fractions and perfusion defects corresponding to the regions. There were 39 segments of fixed (rest) perfusion defects in MIBI SPECT and there was a significant difference in perfusion defect according to the regional wall motion (normal or mild hypokinesia in 23 regions: 26.2±10.8%, severe hypokinesia, akinesia or dyskinesia in 16 regions: 78.2±23.7, p〈0.001). These data indicate there is a significant coupling between the degree of myocardial perfusion and the myocardial functional change in coronary artery disease.

국내 콩에서 발생하는 바이러스 병해의 발생 상황

이영훈 ( Yeong Hoon Lee ),임승택 ( Seung Taek Lim ),윤영남 ( Young Nam Yoon ),전명기 ( Myeong Gi Jeon ),윤홍태 ( Hong Tae Yun ),고종민 ( Jong Min Ko ),이수헌 ( Su Heon Lee ),이기운 ( Key Woon Lee ),백인열 ( In Youl Baek ) 한국콩연구회 2013 韓國콩硏究會誌 Vol.29 No.1

국내 콩에서는 SMV, SbDV, AMV, CMV, CPMV, SYMMV, SYCMV와 PSV가 보고되었다. 과거 이들 바이러스 중에서 SMV가 심각한 피해를 입히고 있으며, 90%이상 우점하는 것으로 보고되었다. 하지만, 최근 SMV의 발생률은 50%정도로 낮아졌으며, SYMMV, SYCMV와 PSV 등에 의한 여러 가지 바이러스 병해가 피해를 주고 있는 것으로 확인되었다. 최근 기후 및 재배환경의 변화와 국제 농산물 교역으로 인해 병 발생 양상이 급속하게 변하고 있음을 나타내는 단적인 예인 것이다. 또한, 새로운 매개층과 전염원의 발생으로 신종 또는 미보고 바이러스 발생이 늘어나고 있지만, 콩과 같은 주요 작물의 병해 발생 상황 및 피해 양상 구명에 대한 연구는 미흡한 실정이다. 이러한 이유로 국내 콩에서 발생하는 바이러스 병해의 확인을 위해 8도19지역에서 193점의 시료를 채집하였다. 채집된 시료들은 SMV, SYMMV, SYCMV, SbDV, PSV, BCMV, AMV, PEMV, CMV, CCMV, TSV, BBWV2, BYMV와 CPMV 14종에 대한 정밀 진단을 위하여 각각의 종특이 프라이머를 이용하여 RT-PCR 진단이 수행되었다. 그 결과 채집된 시료의 86%가 바이러스에 감염된 것으로 확인 되었으며, SMV 141, SYMMV 14, PSV 8과 SYCMV 5점이 확인되었다. 대구와 나주에서 채집된 2점의 시료들에서는 SYMMV와 SYCMV가 복합감염 되어있었다. 나머지 미동정 시료에 대한 정밀 분석과 지속적인 발생상황 조사가 이루어 져야 할 것이다. It had been reported that soybean viral diseases are Soybean mosaic virus (SMV), Soybean dwarf virus (SbDV), Alfalfa mosaic virus (AMV), Cucumber mosaic virus (CMV), Cowpea mosaic virus (CPMV), Soybeun yellow rnottle mosaic virus (SYMMV), Soybean yellow common mosaic virus (SYCMV) and Peanut stunt virus (PSV) in Korea. Among these viral diseases, SMV caused severe damage to soybean in Korea, Although SMV occurred more than 90% in the past, recently several viruses such as SYMMV, SYCMV, SbDV and PSV have been reported in Korea, It means that the incidence of viral diseases are changing in soybean. To identify the viruses infecting soybean in Korea, the 193 samples with viral symptoms were collected in 19 areas of 8 provinces. And then the RT-PCR assay was conducted to detect 14 different viruses such as SMV, SYMMV, SYCMV, SbDV, PSV, BCMV, AMV, PEMV, CMV, CCMV, TSV, BBWV2, BYMV and CPMV. The results indicated that about 86% of samples were identified as Virus-infected, Among 193 soybean samples, 141 SMV, 8 PSV, 14 SYMMV, 5 SYCMV were detected. Two samples were coinfected with SYMMV and SYCMV. The rest of them were likely to express the mosaic similar to virus-induced symptoms. Therefore, the identifications of the unknown samples have been performed by the dererminatlon of the nucleotide sequences of the genomic RNAs.