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Surveying the damage: the challenges of developing nucleic acid biomarkers of inflammation
Son, Junghyun,Pang, Bo,McFaline, Jose L.,Taghizadeh, Koli,Dedon, Peter C. Royal Society of Chemistry 2008 Molecular bioSystems Vol.4 No.9
<P>Epidemiological evidence points to a cause and effect relationship between chronic inflammation and human maladies such as cancer, atherosclerosis and autoimmune disease. A critical link between inflammation and disease may lie in the secretion of highly reactive oxygen and nitrogen species by macrophages and neutrophils, including hypohalous acids, nitrous anhydride, and nitrosoperoxycarbonate. Exposure of host epithelial cells to the resulting oxidation, nitration, nitrosation and halogenation chemistries leads to damage of all types of cellular molecules. Since nucleic acids sustain damage representative of the full spectrum of different chemistries and the damage likely plays a causative role in disease etiology, DNA and RNA damage products can serve as surrogates for the short-lived chemical mediators of inflammation, and as markers that provide both mechanistic understanding of the disease process and a means to quantify risk of disease. However, the very small quantities of the damaged molecules pose a challenge to the simultaneous quantification of the spectrum of lesions in the manner of proteomics or metabolomics. The goal of this Highlight is to provide an update on the chemistry of inflammation and the development of biomarkers of inflammation in the age of -omics technologies.</P> <P>Graphic Abstract</P><P>Chronic inflammation and human disease may be linked by damage to biomolecules such as DNA and RNA, with the spectrum of products reflecting the chemistry of phagocyte-generated reactive species. <IMG SRC='http://pubs.rsc.org/services/images/RSCpubs.ePlatform.Service.FreeContent.ImageService.svc/ImageService/image/GA?id=b719411k'> </P>
Yoo, Hye Hyun,Son, Junghyun,Lee, Jaeick,Kim, Nam Sun,Shin, Myungyoup,Kang, Min-Jung,Kim, Dong-Hyun John Wiley Sons, Ltd. 2006 Rapid communications in mass spectrometry Vol.20 No.13
<P>The metabolism and excretion of 2-methylaminoethoxycarbonyl-4,4′-dimethoxy-5,6,5′,6′-dimethylenedioxybiphenyl-2′-carboxylic acid (DDB-S) were investigated in both rats and humans using liquid chromatography/electrospray ion trap mass spectrometry (LC/ESI-MS/MS). In rats, DDB-S was rapidly eliminated from the body after a single 50 mg/kg intravenous injection, with urine being a major excretion route. DDB-S was metabolically stable; approximately 96% of the administered dose was recovered in the form of the parent compound. Nevertheless, 12 metabolites were detected in the urine and feces collected from DDB-S-treated rats. The structural characterizations of the metabolites were elucidated from the MS<SUP>n</SUP> spectral analysis. Because DDB-S has a pseudo-symmetrical methylenedioxy biphenyl structure, regioselective deuterium-substituted DDB-S (d<SUB>5</SUB>-DDB-S) was used to assign the metabolic modification. The major metabolic pathways of DDB-S were identified as demethylenation of the methylenedioxy moiety, O-demethylation of the methoxy moiety and glucuronidation. In addition, N-demethylation of the methylaminoethyl group was also detected as a minor reaction. Copyright © 2006 John Wiley & Sons, Ltd.</P>
Junghyun Park,Youngsu Ha,Sukbon Yoon,Kiho Son 한국방사성폐기물학회 2023 한국방사성폐기물학회 학술논문요약집 Vol.21 No.1
In Korea, many characteristic component facilities and technologies in general experimental areas for non-radiative materials are owned by industry-academia research. Still, no characteristic analysis test technology has been developed for large, intermediate-level decommissioning waste emitted by neutron irradiation. Since Korea plans to decommission nuclear power plants in 2027, securing analysis technology for intermediate-level decommissioning waste is essential. Accordingly, the Korea Research Institute of Decommissioning (KRID) plans to secure an infrastructure (hot cell) to analyze the characteristics of intermediate-level dismantled waste. Afterward, we intend to stably dispose of the waste generated while decommissioning the current Gori Unit 1/Wolseong Unit 1 using the intermediatelevel dedicated hot cell. It aims to secure high-dose/high-radiation decommissioning waste handling technology through intermediate-level hot cells for the first time in Korea, supports domestic nucleardecommissioning projects, and secure and validate procedures related to material characteristics and nuclide analysis of intermediate-level waste. Furthermore, research on intermediate-level radioactive materials is expected to be carried out in cooperation with schools and research institutes.
Junghyun Park,Eun Kyoung Jun,Daryeon Son,Wonjun Hong,Jihoon Jang,Wonjin Yun,Byung Sun Yoon,Gwonhwa Song,In Yong Kim,Seungkwon You 생화학분자생물학회 2019 Experimental and molecular medicine Vol.51 No.-
Alopecia, one of the most common chronic diseases, can seriously affect a patient’s psychosocial life. Dermal papilla (DP) cells serve as essential signaling centers in the regulation of hair growth and regeneration and are associated with crosstalk between autocrine/paracrine factors and the surrounding environment. We previously demonstrated that amniotic fluid–derived mesenchymal stem cell–conditioned medium (AF-MSC-CM) accelerates hair regeneration and growth. The present study describes the effects of overexpression of a reprogramming factor, Nanog, on MSC properties, the paracrine effects on DP cells, and in vivo hair regrowth. First, we examined the in vitro proliferation and lifespan of AF-MSCs overexpressing reprogramming factors, including Oct4, Nanog, and Lin28, alone or in combination. Among these factors, Nanog was identified as a key factor in maintaining the self-renewal capability of AF-MSCs by delaying cellular senescence, increasing the endogenous expression of Oct4 and Sox2, and preserving stemness. Next, we evaluated the paracrine effects of AF-MSCs overexpressing Nanog (AF-N-MSCs) by monitoring secretory molecules related to hair regeneration and growth (IGF, PDGF, bFGF, and Wnt7a) and proliferation of DP cells. In vivo studies revealed that CM derived from AF-N-MSCs (AF-N-CM) accelerated the telogen-to-anagen transition in hair follicles (HFs) and increased HF density. The expression of DP and HF stem cell markers and genes related to hair induction were higher in AF-N-CM than in CM from AF-MSCs (AF-CM). This study suggests that the secretome from autologous MSCs overexpressing Nanog could be an excellent candidate as a powerful anagen inducer and hair growth stimulator for the treatment of alopecia.
Isolation of mesenchymal stem cells from Pap smear samples
( Junghyun Park ),( Daryeon Son ),( Wonjun Hong ),( Jihoon Jang ),( Geum Joon Cho ),( Gwonhwa Song ),( In Yong Kim ),( Seungkwon You ) 대한산부인과학회 2020 Obstetrics & Gynecology Science Vol.63 No.5
Objective Exploiting their ability to differentiate into mesenchymal lineages like cartilage, bone, fat, and muscle, and to elicit paracrine effects, mesenchymal stem cells (MSCs) are widely used in clinical settings to treat tissue injuries and autoimmune disorders. One of accessible sources of MSC is the samples used for Papanicolaou (Pap) test, which is a cervical screening method for detecting potentially pre-cancerous and cancerous alterations in the cervical cells and to diagnose genetic abnormalities in fetuses. This study aimed to identify and isolate the stem cells from Pap smear samples collected from pregnant women, and to trace the origin of these cells to maternal or fetal tissue, and characterize their stem cell properties. Methods To investigate the possibility and efficiency of establishing MSC lines from the Pap smear samples, we were able to establish 6 cell lines from Pap smear samples from 60 pregnant women at different stages of gestation. Results The 3 cell lines randomly selected among the 6 established in this study, displayed high proliferation rates, several characteristics of MSCs, and the capacity to differentiate into adipocytes, osteocytes, and chondrocytes. Our study identified that the stem cell lines obtainable from Pap smear sampling were uterine cervical stromal cells (UCSCs) and had 10% efficiency of establishment. Conclusion Despite their low efficiency of establishment, human UCSCs from Pap smear samples can become a simple, safe, lowcost, and donor-specific source of MSCs for stem cell therapy and regenerative medicine.