금식시 흰쥐 소장 점막 유당분해효소에 대한 갑상선 호르몬의 영향 및 이의 유전적 조절 기전

이국래,이동호,이한주,윤정환,김재규,정운태,정현채,송인성,김정룡 大韓消化器病學會 1996 大韓消化器病學會誌 Vol.28 No.6

정상과 만성염증성 활막배양세포에서 Corticotropin releasing hormone(CRH)이 cyclooxygenaase-1(COX-1)과 cyclooxygenase-2(COX-2) mRNA발현에 미치는 영향

정운원,이승관,이창규,조경진,김성욱,하주희,서장훈 高麗大學校 倂設 保健大學 保健科學硏究所 2000 保健科學論集 Vol.26 No.1

Corticotropin-releasing hormone(CRH) is a major regulator of the hypothalamic-pituitaryadrenal axis(HPA). In inflammatory stress, the cytokines TNF-, IL-6 stimulate the production of CRH, a 41 amino acids neuropeptide, in the hypothalamus. The release of CRH leads to pituitary production of adrenocorticotropic hormone, followed by glucocorticoid secretion by the adrenal cortex. Glucocorticoids suppress namy components of the inflammatory process. Recently, CRH and CRH receptor were reported to be located in the periphery such as Immune system and chronic inflammatory sites as rheumatoid arthritis (RA). Cyclooxygenase consisted of two isoforms, COX-1 and COX-2, converts arachdonate to prostaglandins(PGs) which are important mediators of inflammation. insymoviocyte in RA, it was described that COX-2 mRNA was markedly increased by inflammatory agents, PMA or IL-1 and COX-1 transcripts were not modulated. We examined the modulation of COX by immune CRH in cultured normal and rheumatoid synoviocytes. Our results were shown that COX-1 mRNA expression decreased with the each stimulation of PMA and IL-1 in normal synoviocyte. In RA synoviocyte, PMA and IL-1 were increased mRNA expression of COX-1. In simultaneous treatment with CRH, PMA group was decreased, but IL-1 group was increased mRNA expression of COX-1. COX-2 mRNA expression was slightly increased by the treatment with PMA and highly increased by IL-1. After CRH treatment, PMA and IL-1 addictively increased COX-2 mRNA expression. We think that these results are contributed to the influence of increased cANP by CRH on the promoter of COX-2 in normal and synoviocytes. Because many cytokines, neuropeptides, and signal transduction pathways are involved in chronic inflammation, the exact role of CRH on inflammation is not fully elucidated. To achieve this goal, further experiments are needed.

Smear layer 처리에 따른 미세누출에 대한 연구

이정민,박상혁,최기운 대한치과보존학회 2006 Restorative Dentistry & Endodontics Vol.31 No.5

본 연구는 도말층 제거 여부에 따른 근관 밀폐효과를 평가하기 위해 단근관 치아를 3% NaOCI 하에서 Ni-Ti file을 이용하여 crown-down 법으로 근관 형성 후 최종세정제로 NaOCI을 사용한 군과 EDTA를 사용한 군, 6개월 보관한 NaOCI-6군과 EDTA-6군으로 분류하였다. Continuous wave법으로 근관 충전 시행 후 색소 침투를 시행한 다음 해부학적 근첨에서 1.5 mm(Level l), 3.0 mm(Level 2), 4.5 mm (Level 3)에서 수평절단 하여 누출률을 측정하였다. 1. 모든 실험군에서 평균 누출률은 치근단부에서 치경부로 갈수록 감소하였다. 2. NaOCI 군의 누출률이 EDTA 군보다 level l, 2, 3에서 높게 나타났으나 level l에서만 통계학적 유의차를 보였다 (p<0.05). 3. NaOCI-6 군의 누출률이 EDTA-6 군보다 Level l, 2, 3에서 높게 나타났으나 level l에서만 통계학적 유의차를 보였다 (p<0.05). 4. NaOCI-6 군의 누출률이 NaOCI 군에 비해 Level l, 2, 3에서 증가하였으나 level l에서만 통계학적 유의차를 보였다 (p<0.05). 5. EDTA-6 군의 누출률이 EDTA 군에 비해 Level l, 2, 3에서 증가하였으나 통계학적 유의차는 없었다. 6. 주사전자현미경 관찰 결과 NaOCI 군과 NaOCI-6 군은 도말층이 제거되지 않아 상아세관내로 sealer 및 근관충전 재가 침투하지 못한 반면, EDTA 군과 EDTA-6 군에서는 도말층이 제거되고 상아세관내로의 sealer와 근관충전 재의 침투가 관찰되었다. 이상의 연구결과 EDTA를 이용하여 도말층을 제거한 경우 근관충전 즉시와 6개월 후 치근단 1/3 부위의 근단부 미세누출을 감소시킬 수 있을 것으로 사료된다. The purpose of this study was to compare the sealing ability of root canal obturation with or without the treatment of smear layer. Eighty extracted human teeth with one canal were selected. Instrumentation was performed with crown-down technique. After instrumentation, root canals of the NaOCI group and NaOC1-6 group were irrigated with 3% NaOCI. EDTA group and EDTA-6 group were irrigated with 17% EDTA. Then all teeth were obturated using continuous wave obturation technique. NaOCI group and EDTA group were immersed in methylene blue solution for 84hours. NaOCI-6 group and EDTA-6 group were immersed in methylene blue solution for 6months. The teeth were sectioned at 1.5 mm (Level 1), 3.0 mm (Level 2) and 4.5 mm (Level 3) from the root apex. The length of dye-penetrated interface and the circumferential length of canal at each level were measured using Sigma-Scan Pro 5.0. 1. The mean leakage ratio was decreased cervically. 2. NaOCI group showed higher mean leakage ratio than EDTA group at each level. But there was significant difference at level 1 only (p<0.05). 3. NaOCI-6 group showed higher mean leakage ratio than EDTA-6 group at each level. But there was significant difference at level 1 only (p<0.05). 4. NaOCI-6 group showed higher mean leakage ratio than NaOCI group at each level. But there was significant difference at level 1 only (p<0.05). 5. EDTA-6 group showed higher mean leakage ratio than EDTA group at each level. But there was no significant difference. 6. In NaOCI group and NaOCI-6 group, scanning electron micrographs of tooth sections generally covered with smear layer. In EDTA group and EDTA-6 group, tooth sections showing the penetration of sealers to opened dentinal tubules. The results suggest that removal of smear layer was effective to reduce the apical microleakage of the root canal.

수용성 항진균성 항생물질을 생산하는 방선균 LAM-593의 분리 및 물질생산조건

이동희,김중배,이노운 建國大學校 附設 産業技術硏究所 1993 논문집 Vol.18 No.-

토양에서 분리 선별한 수용성 항진균성 물질을 생산하는 Streptomyces속 균주 LAM-593에 의한 항진균성 물질의 생산조건을 검토한 결과는 다음과 같다. 물질생산을 위한 탄소원으로는 soluble starch가, 질소원으로는 soybean meal과 peptone이 가장 우수하였으며 glycerol의 첨가로 물질 생성량이 현저히 증가하였으나 인산염에 의해서는 저해되었다. 생산 최적온도와 초발 pH는 각각 30℃와 9.5였으며, soluble starch 1.0%, glycerol 1.0%, soybean meal 0.3%, peptone 0.1%, MgSO₄·7HO 0.05%, NaCI 0.05%, silicone oil 0.01% 조성의 배지(pH 9.5) 2.5L를 5L fermenter에 넣고 전배양한 배양액을 3% 수준으로 접종하여 통기량 0.9vvm, 교반속도 200rpm으로 30℃ 에서 2일간 배양하였을 때 비지내의 항진균성 물질의 축적량이 최대에 달하였다. Cultural conditions for production of water soluble antifungal antibiotic by Streptomyces sp, strain LAM-593, isolated from soil, were studied. For the antibiotic production, soluble starch and soybean meal was favorable as carbon and nitrogen source, respectively. The antibiotic production was stimulated by the addition of Mg? and glycerol. Optimal temperature and initial pH for production of the antibiotic was 30℃ and pH 9.5, respectively. Accumlation of the antibiotic in th culture broth reached at maximum level after 2 days cultivation under aeration rate 0.9 vvm and impeller speed 200rpm in 5L-fermenter with 3L of medium containing soluble starch 1.0%, glycerol 1.0%, soybean meal 0.3%, peptone 0.1%, MgSO₄·7H₄O 0.05% and Nacl 0.05%.

Hall 소자를 이용한 승강기용 로프의 평가

이종오,윤운하,손영호,김정우,이종규 한국비파괴검사학회 2003 학술대회 논문집 Vol.- No.1

홀 소자를 이용한 누설자속 측정으로 승강기용 와이어 로프의 결함 검출에 대한 연구를 수행하였다. 소선파단, 마모, 내부 소선파단 형태의 인공결함을 제작하여 시험편에 영구자석으로 자장을 걸어 결함에서 누설되는 누설자속을 홀 센서를 사용하여 검출하였다. 로프의 소선은 0.5, 0.8mm 두 종류로 시험결과 단선모델 시험에서 0.4mm, 내부의 소선 파단의 경우 1mm 정도 깊이를 가진 인공결함까지 검출이 가능하여 1개의 소선 파단도 검출할 수 있는 것으로 보인다. 마모모델에서 결함의 폭이 작은 경우 검출이 불가능하였으나, 폭이 4mm이상인 경우 자극이 결함을 통과할 때 자속의 변화에 의해 깊이가 0.2mm 정도의 작은 결함도 구별이 가능하였다. 따라서 Hall 센서에 의한 누설자속 측정이 wire rope의 결함 검출에 좋은 도구가 된다고 본다. Defect detection of wire rope for an elevator was investigated through the measurement of magnetic flux leakage. The types of defect usually found in wire rope categorized such as inner and outer wire breakage and wear. The specimens that has artificial defects were magnetized via permanent magnet, and measurement of magnetic flux leakage on the defects was performed with Hall sensor. In wire broken model, a defect smaller than 0.4mm and 1mm in depth on outer and inner wire rope, respectively, could be detected well. In wear model, smaller defect could not be detected clearly, however, appearance of changing of total magnetic flux during magnetic pole of the sensor passing through a defect 0.2mm in depth at 4mm or above width could make possible to detect it. From the results, the measurement via Hall sensor might be useful tool for defect detection of wire rope.

제주도산 가무락, Cyclina sinensis의 생식주기에 관한 조직학적 연구

이정재,조운삼 제주대학교 해양연구소 1985 해양자원연구소연구보고 Vol.9 No.-

DNA Microarray를 이용한 인유두종 바이러스(Human Papilloma Virus:HPV)의 진단

정운원,이승관,이창규,조경진,김성욱 高麗大學校 倂設 保健大學 保健科學硏究所 2001 保健科學論集 Vol.27 No.1

Human papillomavirus(HPV) has been known as one of the important pathogenic agent in uterine cervical carcinoma. The molecular works such as PCR enable the detection of large number of HPV genotypes obtained from viginal swab. Many of the PCR-based methods for HPV detection involve an amplification step followed by any of a number of methods for distinguishing different HPV types. In this study, we adopted the DNA chip technology enabling a HPV type-specific differentiation both low-risk group(type-6, 11, 34, 40, 42, 43, and 44) and high-risk group(type-16, 18, 31, 33, 35, 39, 45, 51,52,54, 56, and 58). MY09/MY11 and GP5+/GP6+primers covered LI region are used in nested PCR to improve PCR amplification. HPV type-specific probes for DNA chip were modified with NH2-C6, followed by spotting on silylated slides, washing slides and hybridization with each PCR products. Of 163 DNA samples chosen randomly, 42 samples were negative, 8 ones for low-risk group of HPV and 96 ones for high-risk group of HPV. Especially, co-infections with various HPVs were shown in 17 samples. A recent study found that multiple HPV is a factor in persistent HPV infection, resulting in the development of cervical dysplasia. This result emphasized the necessity to detect multiple HPV infection. The application of DNA-chip to determinate specific HPV typing will be a stronger candidate than any other PCR-based methods. Furthermore, the sequencing data of the positive PCR products were shown no discrepancy with DNA chip results. This means that DNA chip is very useful tool for both HPV detection and typing.

일측전정기과 손상 희쥐의 전정보상과정에서 뇌간 신경핵의 cAMP-Response Element Binding 단백질 활성화

윤상대,이정헌,김진호,김민선,박병림 대한이비인후과학회 2001 대한이비인후과학회지 두경부외과학 Vol.44 No.12

구획 화재시 창유리 파괴 현상에 관한 실험적 연구

이수경,김종훈,최종운,이정훈 韓國火災ㆍ消防學會 1998 한국화재소방학회논문지 Vol.12 No.3

본 연구는 실제 화재실험을 통하여 구획 화재 시 일어날 수 있는 창유리의 파괴 형태 및 시간 그리고 개구 조건과의 관계 등을 고찰하고자 했다. 화재 시 창유리의 파괴현상은 창호 유리 중간 부분과 프레임 안에 있는 유리부분의 현저한 온도차이로 인한 열응력으로 일어남을 확인했다. 실험 1-3은 프레임 내부와 유리면의 온도차가 233.4℃ 였으며, 실험 2-1은 138℃, 실험 2-2는 83.6℃의 차이를 보였다. 파괴 발생 시 실내의 온도는 실험 2-1의 경우 434.4℃, 실험 2-2는 83.6℃의 차이를 보였다. 파괴 발생 시 실내의 온도는 실험 2-1의 경우 434.4℃, 실험 2-2는 440.6℃ 실험 2-3은 400.9℃를 기록하여 화염이 직접 닿지 않는 경우의 균열은 400 ~ 500℃의 온도에서 발생한다. 화재로 인한 창유리(한글라스 플로팅 유리)는 1541.14kW의 화재에 노출되었을 시 열응력에 의한 파괴가 일어나기는 하나 유리면이 파괴에 의해 개구부가 되지 않는다. This is a study on the relation of window glass breaking time, shape, and vent condition in a compartment fire through the experiment. We recognize the phenomenon that window glass breakage in a compartment fire be arose from the thermal stresses due to the temperature temperature difference was 233.4℃ for test 1-3, 138℃ for text 2-1, 83.6℃ for test 2-2. The interior test 2-2, 400.9℃ for test 2-3. so if the flame didn't reach at the surface of window glass, the breakage of glass occur at 400℃ ~ 500℃. When the fire size reached to 1541.14kW, the window might be broken by thermal stress. But window glass was not collapsed.

WDM 시스템용 광대역 증폭기의 시뮬레이션

李鎬俊,沈雲用,鄭白鎬 湖西大學校工業技術硏究所 1999 工業技術硏究所論文集 Vol.18 No.-

In recent years, the biggest issue of WDM system is in increasing its bandwidth. Transmission bandwidth of WDM may be limited by many kinds of component, and the bandwidth plays impotent role in most of all optical amplifiers. Transmission bandwidth of recent WDM is limited in about 40 nm. This limit depends on EDFA(?? Fiber Amplifier) gain bandwidth. To overcome this problem, we may use L and S band using Yb/Nd doped fiber amplifier. Wideband amplifier, which is consisted of RFA for L band and EDFA for M band using single pump laser diode, is presented. The amplifiers have been analyzed numerically for the design.