Complete genome sequence and comparative analysis of Streptomyces seoulensis, a pioneer strain of nickel superoxide dismutase

Jihoon Shin,Shinae Park,Jung‑Shin Lee,Eun‑Jin Lee,Hong‑Duk Youn 한국유전학회 2020 Genes & Genomics Vol.42 No.3

Background Streptomyces seoulensis has contributed to the discovery and initiation of extensive research into nickel superoxide dismutase (NiSOD), a unique type of superoxide dismutase found in actinomycetes. Still so far, there is no information about whole genome sequence of this strain. Objective To investigate complete genome sequence and perform bioinformatic analyses for genomic functions related with nickel-associated genes. Methods DNA was extracted using the Wizard Genomic DNA Purification Kit then sequenced using a Pacific Biosciences SMRT cell 8Pac V3 DNA Polymerase Binding Kit P6 with the PacBiov2 RSII platform. We assembled the PacBio longreads with the HGAP3 pipeline. Results We obtained complete genome sequence of S. seoulensis, which comprises a 6,339,363 bp linear chromosome. While analyzing the genome to annotate the genomic function, we discovered the nickel-associated genes. We observed that the sodN gene encoding for NiSOD is located adjacent to the sodX gene, which encodes for the nickel-type superoxide dismutase maturation protease. In addition, several nickel-associated genes and gene clusters-nickel-responsive regulator, nickel uptake transporter, nickel–iron [NiFe]-hydrogenase and other putative genes were also detected. Strain specific genes were discovered through a comparative analysis of S. coelicolor and S. griseus. Further bioinformatic analyses revealed that this strain encodes at least 22 putative biosynthetic gene clusters, thereby implying that S. seoulensis has the potential to produce novel bioactive compounds. Conclusion We annotated the genome and determined nickel-associated genes and gene clusters and discovered biosynthetic gene clusters for secondary metabolites implying that S. seoulensis produces novel types of bioactive compounds.

조혈모세포이식 후 발생한 주폐포자층 폐렴에 대한 고찰

주지현,최정현,이동건,백지연,고윤호,이혜정,김세희,신호진,박윤희,박지영,김유진,신완식,김춘추 대한감염학회 2001 감염 Vol.33 No.4

Background : Pneumocytitis cainii pneumonia (PCP) can occur in immunocompromised hosts especially such as AIDS or cancer patients. Although recent research had focused on PCP in AIDS patients, few studies have described the clinical presentations of PCP in recipients of stem cell transplantation (SCT). We evaluated the clinical manifestations of PCP in SCT patients admitted at St. Mary's hospital, Seoul, Korea. Methods : The medical records of 17 PCP patients undergoing SCT between Feb. 1998 and Feb. 2000 were reviewed. The diagnosis of PCP was confirmed through the demonstration of Pneumocytitis cainii via either cytology of brochoalveolar lavage (BAL) or histological technique of lung biopsy. CMV disease and CMV infection were confirmed by BAL culture and antigenemia respectively . Results : Seventeen patients were all recipients of allogeneic SCT and 7 of 17 patients were performed non-sibling SCT. Patients presented with symptoms including brief period (4 ∼23 days) of fever (76%), dyspnea (70%), cough (64%), and signs such as rare(58.8%), Sixteen patients (94%) had been receiving immunosuppressive agent such as cyclosporine A (64%) or Fk506 (35%) without PCP prophylaxis. Eleven patients (64%) were treated with corticosteroid with mean dose of 16 mg/day prednisolone and mean duration of 4.6 months after post-SCT period. Twelve patients were co-infected with CMV. Another co-infected miCroorganisms were Pseudomonas aeruginosa, Mycobacterium tuberculosis, herpes simplex virus, parainfluenza virus, Average duration of treatment with trimethoprim-sulfamethoxazole (TMP/SMX) was 21 ±9 days. Four patients died, and three of them were related with PCP. Conclusion : PCP developed frequently in patients who were taking immunosuppressive drug due to graft versus host disease or were not taking TMP/SMX prophylaxis. High risk patients showing fever, cough, or dyspnea should be considered to take early bronchoscopic intervention for detection of PCP. When treat for PCP, it also be considered to the possibility of coinfection such as CMV. (Korean J Infect Dis 33:273∼279, 2001)

자료포락분석(DEA)를 이용한 보건의료의 효율성 측정

김남송,박홍섭,김은진,김설화,김정숙,김현아,김경숙,신지혜,최은정,양은진,유선미,유수진 圓光大學校 醫科學硏究所 2007 圓光醫科學 Vol.22 No.1

쓰레기 매립장 주변 농촌 주민들의 삶의 질 연구

이명경,최준열,김인경,조영아,김영신,정혜진,김리나,이영규,조영태,Lee, Myung-Kyung,Choi, Jun-Yeol,Kim, In-Kyoung,Cho, Yeong-Ah,Kim, Young-Shin,Jung, Hye-Jin,Kim, Li-Na,Lee, Young-Kyu,Cho, Young-Tae 대한예방의학회 2006 예방의학회지 Vol.39 No.4

Objectives: This study aims to examine if a garbage dumping site has real and negative influence on the quality of life (QOL) for the nearby residents. The net effects of the residential distance from the garbage dumping site and from the garbage truck route were investigated for five domains of the QOL. Methods: Two hundred fifty seven Shin-dong Myeon residents, Chun-cheon Si, participated in a self-administrated survey. The Shin-dong Myeon garbage dumping site began operating in 1996. ANCOV A with generalized linear models and multiple regression analysis were performed. Results: Descriptive analyses show that a residence nearby a garbage dumping site is negatively associated with the physical and environmental domains of the QOL. The residential distance from the garbage truck route does not exert any significant effect on various domains of QOL, except for the environmental domain. On the multivariate analysis, the residents living near the garbage dumping site tended to have a significantly negative QOL in the physical and environmental domains. However, the distance from the garbage truck route did not show a significant nor substantial effect on the QOL. The demographic and socioeconomic control variables are associated with a number of the QOL domains, and their patterns are consistent with the general expectations. Conclusions: The results indicated that a garbage dumping site is considered to be an environmental hazard among the nearby residents according to the lower scores on the physical and environmental domains of the QOL. The findings from this study provide comprehensive understanding on the residents' QOL, and they may help politicians and policy makers make decisions for appropriate interventions.

중부지역 과수원 토양중의 중금속 함량 평가

정구복,김원일,이종식,신중두,김진호,윤순강 한국환경농학회 2004 한국환경농학회지 Vol.23 No.1

우리나라 중부지역에서 1998년 3∼5월에 과수원 토양 164지점(경기 48, 강원 36, 충북 36, 충남 44지점)을 대상으로 표토(0∼20 ㎝)와 심토(20∼40 ㎝)로 나누어 채취하여 토양내 중금 속함량과 분포특성, 총함량에 대한 침축액별 가용성 함량 비율 및 토양 이화학성과의 관계 를 비교 검토한 결과는 다음과 같다. 과수원 토양중 0.1 N-HCl 침출성 평균함량은 Cd 0.080, Cu 4.23. Pb 3.42 ㎎/㎏, 1 N-HCl 침출성 As 평규함량은 0.44 ㎎/㎏, 중금속 총함량 은 Zn 78.9, Ni 16.09 및 Hg 0.052 ㎎/㎏ 이었다. 과수원 토양내 중금속 평균함량은 우리 나 라 토양환경보전법의 토양오염 우려기준(Cd 1.5, Cu 50, Pb 100, Zn 300, Ni 40, Hg 4 ㎎/ ㎏)과 비교하여 1/25∼1/76.9 수준으로 안전하였다. 토양의 중금속 총함량에 대한 침출액별 가용성 함량비율은 Cd 5.4∼9.2, Cu 27.9∼47.8, Pb 12.6∼21.8, Zn 15.8∼20.3, Ni 5.3∼6.3, Cr 0.7∼3.6% 이었고, 특히 0.05 M-EDTA 침출성 CU 및 Pb의 침출비율이 상대적으로 높 게 나타났다. 토양내 Cd, Pb 및 Ni의 총함량은 모래함량과 부의상관, 미사와 점토함량과는 정의 상관을 보였다. 토양의 중금속 총함량에 대한 침출액별 가용성 함량비율은 점토함량과 는 부의 상관을 보였으며, Zn과 Ni의 함량비율은 토양 pH값, 유기물 및 유효인산 함량과 정 의 상관을 보였다. 이상의 결과에서 볼 때 과수원 토양의 중금속 함량은 토양환경보전법의 토양오염기준보다 매우 낮아 안전하였으나 영농활동에 의한 영향으로 볼 수 있는 농도수준 이 검출된 일부 토양에서 조사되었다. 따라서 최근의 친환경농업 측면으로 볼 때 영농형태 별 중금속의 분포 및 농업자재에 의한 농경지내 중금속 부하량에 근거하여 중금속 오염유무 를 평가할 수 있는 판단기준에 대한 연구가 필요하다고 생각된다. Objectives of this study were to monitor the distribution of heavy metals, to compare extractable heavy metal with total content and to investigate the relationships between soil physico-chemical properties and heavy metals in orchard soil. sampling sites were 48 in Gyeonggi, 36 in Gangwon, 36 in Chungbuk, and 44 in Chungnam. Soils were collected form two depths, 0 to 20 and 20 to 40 cm (here after referred to as upper and lower layers) from March to May in 1998. Total contents of heavy metal in soils were analyzed by ICP-OES after acid digestion (HNO₃:HCl:H₂O₂) whereas extractable contents were measured after successive extraction of 0.1 N-HCl, 0.05 M-EDTA, and 0.005 M-DTPA. Mercury was analysed by mercury atomizer. The average contents of Cd, Cu, and Pb in the extractant with 0.1 N-HCl at upper layer were 0.080, 4.23, and 3.42 ㎎/㎏, respectively. As content in the extractant with 1 N-HCl was 0.44 ㎎/㎏, and total contents of 20, Ni, and Hg were 78.9, 16.1, and 0.052 ㎎/㎏, respectively. m e ratios of concentrations of heavy metals to threshold values (Cd 1.5, Cu 50, Pb 100, Zn 300, Ni 40, Hg 4 ㎎/㎏) in soil Environmental Conservation Act in Korea (2001) were low in the range of 1/2.5-1/76.9 in orchard soils. The ratios of extractable heavy metal to total content ranged 5.4-9.21% for Cd, 27.9-47.8% for Cu, 12.6-21.8% for Pb, 15.8-203% for Zn, 5.3 -6.3% for Nii and 0.7 -3.6% for 2n, respectively. Cu and Pb contents in 0.05 M-EDTA extractable solution were higher than those in the other extractable solution. Total contents of Cd, Ni, and Ni in soils were negatively correlated with sand content, but positively correlated with silt and clay contents. Ratios of extractable heavy metal to total content were negatively correlated with clay content, but Zn and Ni contents were positively correlated with soil pH, organic matter, and available phosphorous. Therefore, the orchard soil was safe because the heavy metal contents of orchard soil were very low as compared to its threshold value in the Soil Environmental Conservation. Act However, it need to consider the input of agricultural materials to the agricultural land for farming practices for assessment of heavy metals.

'-ㄹ]Noun+ㅅ+Noun' 合成語의 通時 語彙論

愼重珍 국어학회 2004 국어학 Vol.0 No.44

오늘날의 '칼날, 글귀, 길가, 물결, 물고기, 밀가루, 발톱, 솔방울, 활시위, 뭇사람, 이튿날, 사흗날, 일기, 바닷가, 바닷물, 송진, 햇빛'과 같은 어휘는 각각 중세국어 시기의 '?Fㄴㆍㄹㅎ, ?G句, ?zㄱㆍㅅ, ?逵? ?逵慈? ?Bㄱㆍㄹㆍ, ?M톱, ??방올, ?시울, ?O사ㄹㆍㅁ, 이?날, 사ㅇㆍㄽ날, ??氣運, 바ㄹㆍㄽㄱㆍㅅ, 바ㄹㆍㄽ믈, ??진, ??빛' 등에 대응되는데, 모두 '-ㄹ]Noun+ㅅ+Noun'의 동일한 內的 構造를 갖는다. 본고에서는 오늘날 상이한 구조의 어휘들이지만, 기원적으로는 동일한 구조의 어휘들을 相同構造의 어휘라고 부른다. 결과적으로 字素形을 중심으로 볼 때, '-ㄹ]Noun+ㅅ+Noun'이었던 어형들은 크게 세 가지 유형으로 심리 어휘부(mental lexicon)에 전승·저장되었는데, 'ㄹ' 유지형인 '칼날, 글구/글귀, 길가, 물결, 물고기, 밀가루, 발톱, 솔방울, 활시위' 등, 'ㄹ' 탈락형인 '뭇사람; 이튿날, 사흗날' 등, 그리고 대체형인 '일기(日氣), 바닷가, 바닷물, 송진(松津), 햇빛' 등이 그것이다. 즉 이들은 원래 상동구조였으나 전승·저장되는 과정에서 심리 어휘부 속의 어근 어휘부나 접사 어휘부의 각기 다른 간섭의 결과로 서로 상이한 내적 구조 유형에 속하게 된 것이다. 본고에서는 현대국어 시기의 어형을 바탕으로 나눈 이 세 가지 유형에 따라 어근 어휘부와 접사 어휘부가 이들의 변화에 각각 어떻게 간섭하였는지를 밝힌다. This paper aims to take a close look at '-ㄹ]Noun+ㅅ+Noun' compounds that appered upon the scene of history variously. For example, '?Fㄴㆍㄹㅎ, ?G句, ?zㄱㆍㅅ, ?逵? ?逵慈? ?Bㄱㆍㄹㆍ, ?M톱, ??방올, ?시울, ?O사ㄹㆍㅁ, 이?날, 사ㅇㆍㄽ날, ??氣運, 바ㄹㆍㄽㄱㆍㅅ, 바ㄹㆍㄽ믈, ??진, ??빛'. As far as '-ㄹ]Noun+ㅅ+Noun' compounds is concerned, we denominate lexemes of the etymologically same structure lexemes of "the homologous structure(相同構造)". From the standpoint of a grapheme, '-ㄹ]Noun+ㅅ+Noun' compounds are divided into three groups, which have been transmitted to the mental lexicon. For example, '칼날(the blade of a knife), 글구/글귀(passage), 길가(roadside), '물결(wave), 물고기(fish), 밀가루(wheat flour), 발톱(toenail), 솔방울(pine cone), 활시위(bowstring)' etc belong to 'ㄹ' maintenance group, '뭇사람(crowd); 이튿날(next day), 사흗날(third day)' etc belong to 'ㄹ' omission group, and '일기(weather), 바닷가(beach), 바닷물(sea water), 송진(pine resin), 햇빛(sunshine)' etc belong to alternation group. Because the root lexicon and the affix lexicon of the mental lexicon have interfered in '-ㄹ]Noun+ㅅ+Noun' compounds. Accordingly they have been belonged to the unlike internal structures.

Conceptual Study for Tissue-Regenerative Biodegradable Magnesium Implant Integrated with Nitric Oxide-Releasing Nanofi bers

Jin‑Kyung Jeon,Hyunseon Seo,Jimin Park,Soo Ji Son,Yeong Rim Kim,Eun Shil Kim,Jong Woong Park,Woong‑Gyo Jung,Hojeong Jeon,Yu‑Chan Kim,Hyun‑Kwang Seok,Jae Ho Shin,Myoung‑Ryul Ok 대한금속·재료학회 2019 METALS AND MATERIALS International Vol.25 No.4

The excessive initial corrosion rate of Mg is a critical limitation in the clinical application of biodegradable Mg implantsbecause the device loses its fi xation strength before the fractured bone heals. This study suggests a new approach to overcomethis hurdle by accelerating tissue regeneration instead of delaying the implant biodegradation. As angiogenesis is anessential process in early bone regeneration, a Mg implant coated with electrospun nanofi bers containing nitric oxide (NO),which physiologically promotes angiogenesis, is designed. The integrated device enables adjustable amounts of NO to bestored on the NO donor-conjugated nanofi ber coating, stably delivered, and released to the fractured bone tissue near theimplanted sites. An in vitro corrosion test reveals no adverse eff ect of the released NO on the corrosion behavior of the Mgimplant. Simultaneously, the optimal concentration level of NO released from the implant signifi cantly enhances tube networkformation of human umbilical vein endothelial cells without any cytotoxicity problem. This indicates that angiogenesis canbe accelerated by combining NO-releasing nanofi bers with a Mg implant. With its proven feasibility, the proposed approachcould be a novel solution for the initial stability problem of biodegradable Mg implants, leading to successful bone fi xation.

LCA 기법을 적용할 제품의 우선순위 선정 방법론

신동희,권은선,문승식,김만영,정종연,허진호 한국전과정평가학회 2004 한국전과정평가학회지 Vol.5 No.1

본 연구의 목적은 일정한 논리적 절차를 거쳐서 객관적인 전과정평가의 우선순위를 선정하기 위한 일반적인 방법론의 개발이다. 우선 전과정평가 대상이 되는 제품(산업군)의 우선순위를 평가하기 위해서 제품의 환경성과 관련된 환경규제, 국내외 전과정평가 수행사례, 유해물질 등을 평가항목으로 설정하였다. 설정된 평가항목들에 대한 자료조사 및 분석을 통하여 세분화한 데이터베이스를 생성하였으며, 또한 학계 및 산업계의 의견을 반영하기 위해 전과정평가 전문가 및 산업계 관계자들을 대상으로 설문조사를 실시하였다. 설문조사결과를 바탕으로 각각의 평가항목 및 세부평가항목의 가중치를 부여하였고, 각 평가항목별로 점수를 설정하였다. 본 연구에서 개발한 방법론은 우선순위를 비교해야하는 제품(산업군)들이 각각의 평가항목에서 설정해놓은 해당 점수를 부여받게 된다. 부여받은 각 평가항목의 점수들을 모두 합산하면 평가대상이 되는 제품(산업군)에 대해 하나의 우선순위 평가 점수를 갖게 된다. 이와 같은 과정으로 방법론이 구축되었으며, 사례연구를 통하여 방법론의 타당성을 검증하였다. 이렇게 산출된 우선순위 평가점수를 평가하고자하는 제품(산업군)들과 비교하여 높은 점수를 갖는 제품(산업군)이 전과정평가를 먼저 실시하는 것이 바람직하다는 결론을 얻을 수 있다. The purpose of this study is to develop a general method to decide the priority of LCA application through a logical procedure. First of all, evaluation categories were developed to decide priority of subject products(industrial groups). The evaluation categories consist of environment regulations related to subject product, LCA case studies, hazardous substances, etc. The subdivided database was constructed through researching and analyzing information about the selected evaluation categories. In addition, a questionnaire was conducted to reflect the opinions from academic and industrial circles. Based on the result of the questionnaire, each evaluation category and sub-evaluation category were weighed differently, and certain points were assigned to them. According to the method developed in this study, comparing industrial groups or products for prioriting will have its total score that is a sum of points assigned to each evaluation category. This results that an industrial group or product having higher score than the others becomes the subject to apply LCA.

韓國 衣類學 硏究의 現況과 再照明 : 1959∼1990

鄭燦辰,朴信貞,黃善珍 한국의류학회 1991 한국의류학회지 Vol.15 No.1

Clothing and Textiles was introduced in the 1950s to Korea and has been developed. At this moment, it seems to be valuable to identify state of art of researches in clothing and textiles field. The purpose of the study was to investigate trends of subject-matter emphasis in clothing and textiles. The data were included clothing and textiles related research articles published in three professional journals from 1959 through 1990 and condensed at 5 year intervals. The identified 620 articles with clothing and textiles subject-matter emphasis were categorized in six areas: clothing construction, textiles, history of costume, design and aesthetics, sociopsychological aspect of clothing, and fashion merchandising. The results were as follows: 1. Since 1959, there has been a significant growth in terms of the number of research as well as in quality of research particulary considering the short history of the field. 2. The number of each area research was ranked as follows: 1) textiles (217) 2) history of costume (173) 3) socio-sychological aspect of clothing (88) 4) clothing construction (79) 5) fashion merchandising (34) 6) design and aesthetics (22) and others (7) 3. In the area of textiles, the most dominant area was clothing management (102 out of 217) and clothing hygenics research was getting increased from the late of 1980 through journal of the Korean Society of Clothing and Textiles. 4. In the area of history of costume, most of the research have been published through Journal of Korean Costume Society. History of korean costume was the most dominant area (120 out of 173) and history of eastern costume area was getting increased from the late of 1980s. 5. In the area of socio-psychological clothing, the research was accelated in the beginning of 1980s through Journal of the Korean Society of Clothing and Textiles, while the research was decreased a little in the late of 1980s. 6. In the area of clothing construction, it was revealed its decrease the percentage of total number of research and most of them were published through journal of Korean Home Economics. 7. In the area of fashion merchandising, there has been continuous increase in the number of research from the late of 1970s to 1990, present. For the future direction, implications for interdisciplinary and ecological approach were suggested.

동하중을 받는 FRAME 구조물의 확률유한요소해석 및 신뢰성해석

신재철,김진홍,정인수,한성호 충남대학교 산업기술연구소 2003 산업기술연구논문집 Vol.18 No.1

Most dynamic systems have various random properties in excitation and system parameters. Therefore, in this paper, reliability analysis for structural response is proposed fro the linear dynamic system with random properties in both excitation and system parameters. The system parameters and responses with random properties are modeled by the perturbation technique. A displacement and bending moment of frame structure is acquired in Stochastic Finite Element Method Program(SFEMP) and Dynamic Stochastic Finite Element Method Program(DSFEMP) using perturbation method, and Direct Monte Carlo Simulation Program(DMCSP) is used to verify and compare previous results. Comparing outputs of SFEMP and DSFEMP with them of DMCSP, it show that they are similar to each other. Also DMCSP need much time to analyze a complicated structure, but SFEMP and DSFEMP can reduce quite time. In this study, it is assumed that excitations, system parameters and response are Gaussian. The results of response for the analysis are compared to those of numerical simulation. Also the probability of failure are calculated for using Advanced First Order Second Moment(AFOSM) of the general reliability analysis method.