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- 저자 : ( Ju An Zhang ) (검색결과 311 건)
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Isoforms of wild type proteins often appear as low molecular weight bands on SDS-PAGE.
Zhang, Ju,Lou, Xiaomin,Shen, Haihong,Zellmer, Lucas,Sun, Yuan,Liu, Siqi,Xu, Ningzhi,Liao, D Joshua Wiley 2014 Biotechnology Journal Vol.9 No.8
<P>Immunoblotting, after polyacrylamide gel electrophoresis with sodium dodecyl sulfate (SDS-PAGE), is a technique commonly used to detect specific proteins. SDS-PAGE often results in the visualization of protein band(s) in addition to the one expected based on the theoretical molecular mass (TMM) of the protein of interest. To determine the likelihood of additional band(s) being nonspecific, we used liquid chromatography - mass spectrometry to identify proteins that were extracted from bands with the apparent molecular mass (MM) of 40 and 26 kD, originating from protein extracts derived from non-malignant HEK293 and cancerous MDA-MB231 (MB231) cells separated using SDS-PAGE. In total, approximately 57% and 21% of the MS/MS spectra were annotated as peptides in the two cell samples, respectively. Moreover, approximately 24% and 36.2% of the identified proteins from HEK293 and MB231 cells matched their TMMs. Of the identified proteins, 8% from HEK293 and 26% from MB231 had apparent MMs that were larger than predicted, and 67% from HEK293 and 37% from MB231 exhibited smaller MM values than predicted. These revelations suggest that interpretation of the positive bands of immunoblots should be conducted with caution. This study also shows that protein identification performed by mass spectrometry on bands excised from SDS-PAGE gels could make valuable contributions to the identification of cancer biomarkers, and to cancer-therapy studies.</P>
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Analysis of Factors Impacting Atmospheric Pressure Plasma Polishing
Ju-Fan Zhang,Bo Wang,Shen Dong 한국정밀공학회 2008 International Journal of Precision Engineering and Vol.9 No.2
Atmospheric pressure plasma polishing (APPP) is a noncontact precision machining technology that uses low temperature plasma chemical reactions to perform atom-scale material removal. APPP is a complicated process, which is affected by many factors. Through a preliminary theoretical analysis and simulation, we confirmed that some of the key factors are the radio frequency (RF) power, the working distance, and the gas ratio. We studied the influence of the RF power and gas ratio on the removal rate using atomic emission spectroscopy, and determined the removal profiles in actual operation using a commercial form talysurf. The experimental results agreed closely with the theoretical simulations and confirmed the effect of the working distance. Finally, we determined the element compositions of the machined surfaces under different gas ratios using X-ray photoelectron spectroscopy to study the influence of the gas ratio in more detail. We achieved a surface roughness of Ra 0.6 ㎚ on silicon wafers with a peak removal rate of approximately 32 ㎣/min.
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Analysis of Factors Impacting Atmospheric Pressure Plasma Polishing
Zhang, Ju-Fan,Wang, Bo,Dong, Shen Korean Society for Precision Engineering 2008 International Journal of Precision Engineering and Vol.9 No.2
Atmospheric pressure plasma polishing (APPP) is a noncontact precision machining technology that uses low temperature plasma chemical reactions to perform atom-scale material removal. APPP is a complicated process, which is affected by many factors. Through a preliminary theoretical analysis and simulation, we confirmed that some of the key factors are the radio frequency (RF) power, the working distance, and the gas ratio. We studied the influence of the RF power and gas ratio on the removal rate using atomic emission spectroscopy, and determined the removal profiles in actual operation using a commercial form talysurf. The experimental results agreed closely with the theoretical simulations and confirmed the effect of the working distance. Finally, we determined the element compositions of the machined surfaces under different gas ratios using X-ray photoelectron spectroscopy to study the influence of the gas ratio in more detail. We achieved a surface roughness of Ra 0.6 nm on silicon wafers with a peak removal rate of approximately 32 $mm^{3}$/min.
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Control of a Laboratory 3-DOF Helicopter: Explicit Model Predictive Approach
Ju Zhang,Xinyan Cheng,Jiaqi Zhu 제어·로봇·시스템학회 2016 International Journal of Control, Automation, and Vol.14 No.2
A helicopter flight control system is a typical multi-input, multi-output system with strong channel couplingand nonlinear characteristics. This paper presents an explicit model predictive control (EMPC) for attituderegulation and tracking of a 3-Degree-of-Freedom (3-DOF) helicopter. A state-space representation of the systemis established according to the characteristics of each degree-of-freedom motion. Multi-Parametric Quadratic Programming(MPQP) and online computation processes for explicit model predictive control and controller design fora 3-DOF helicopter are discussed. The controller design for set-point regulation and tracking time-varying referencesignals of a 3-DOF helicopter are presented respectively. Numerical study of explicit model predictive controlfor attitude regulation and tracking of a 3-DOF helicopter are conducted. A hardware-in-the-loop experimentalstudy of explicit model predictive control of a 3-DOF helicopter is made. To analyze the performances of an EMPCcontrolled helicopter system, an Active Mass Disturbance System and manual interference are considered in comparisonwith PID scheme. Numerical simulation and HIL experimental studies show that explicit model predictivecontrol is valid and has satisfactory performance for a 3-DOF helicopter.
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Zhang, Cheng,Ju, Haoran,Zhang, Dalin,Wu, Shuijin,Xu, Yijun,Wu, Yingwei,Qiu, Suizheng,Su, G.H. Korean Nuclear Society 2020 Nuclear Engineering and Technology Vol.52 No.5
The flow characteristics of reactor fuel assembly always intrigue the designers and the experimentalists among the myriad phenomena that occur simultaneously in a nuclear core. In this work, the visual experimental method has been developed on the basis of refraction index matching (RIM) and particle image velocimetry (PIV) techniques to investigate the detailed flow characteristics in China fast reactor fuel subassembly. A 7-rod bundle of simulated fuel subassembly was fabricated for fine examination of flow characteristics in different subchannels. The experiments were performed at condition of Re=6500 (axial bulk velocity 1.6 m/s) and the fluid medium was maintained at 30℃ and 1.0 bar during operation. As for results, axial and lateral flow features were observed. It is shown that the spiral wire has an inhibitory effect on axial flow and significant intensity of lateral flow mixing effect is induced by the wire. The root mean square (RMS) of lateral velocity fluctuation was acquired after data processing, which indicates the strong turbulence characteristics in different flow subchannels.
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Nonlinear Hybrid Controller Design for Perturbed Quadrotor Robot by Uncertainties
Ju-Qian Zhang,Yu-Ru Shi,Yong Wu,Bang-chun Wen 한국정밀공학회 2018 International Journal of Precision Engineering and Vol.19 No.11
In this paper, a novel nonlinear hybrid observers (HOB) based dynamic surface control (DSC) system, which integrates nominal and compensation controller, is designed for autonomous quadrotor robot perturbed by the uncertainties, e.g., external disturbances and measurement-delay. In this hybrid control system, the nominal controller based on dynamic surface control superior to the backstepping technique is the main controller, and the disturbance observer (DOB) is incorporated as the compensation controller to eliminate the effect of uncertainties caused by model mismatch and external disturbances. In addition, in order to relax the impact of measurement-delay induced by sensors of poor quality and heavy computation burden, the measurement-delay observer (MOB) is derived. Next, stability of the closed-loop control system is analytically proved based on Lyapunov theorem. Finally, comparative controllers are evaluated on simulation and experimental environments to demonstrate the effectiveness and merits of the proposed controller for quadrotor robot against the uncertainties.
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CXCL-13 Regulates Resistance to 5-Fluorouracil in Colorectal Cancer
Guolin Zhang,Xin Luo,Wei Zhang,Engeng Chen,Jianbin Xu,Fei Wang,Gaoyang Cao,Zhenyu Ju,Dongai Jin,Xuefeng Huang,Wei Zhou,Zhangfa Song 대한암학회 2020 Cancer Research and Treatment Vol.52 No.2
Purpose 5-Fluorouracil (5-Fu) is used as a conventional chemotherapy drug in chemotherapy for patients with advanced colorectal cancer, but many patients still suffer from treatment failure due to 5-Fu resistance. Emerging observations revealed the important role of chemokine (C-X-C motif) ligand 13 (CXCL-13) in tumor microenvironment and its relationship with prognosis in patients with colorectal cancer. This study is designed to reveal the important role of CXCL-13 in causing colorectal cancer resistance to 5-Fu. Materials and Methods CXCL-13 levels of patient's serum or cell culture supernatants were measured separately by enzyme-linked immunosorbent assay. In cell assays, cell viability is detected by Cell Counting Kit-8. Therefore, the recombinant human CXCL-13 was used to simulate its high expression in cells while its antibody and siRNA were used to reduce CXCL-13 expression in cells. Results In this study, we demonstrated that CXCL-13 is associated with 5-Fu resistance by culture medium exchange experiments and cytokine arrays of colorectal cancer resistant and nonresistant cells. Clinical studies showed that CXCL-13 is highly expressed in the serum of 5-Fu–resistant patients. High levels of serum CXCL-13 also predict a worse clinical outcome. The addition of recombinant CXCL-13 cytokine resulted in 5-Fu resistance, while its antibody overcame 5-Fu resistance, and knockdown of CXCL-13 expression by siRNA also reduced 5-Fu resistance, which can be saved by added recombination CXCL-13. Conclusion These results not only identify a CXCL-13 mediated 5-Fu resistance mechanism but also provide a novel target for 5-Fu–resistant colorectal cancer in prevention and treatment strategies.
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Dong-Ju Chen,Li-Hua Yan,Qian Li,Cai-jiao Zhang,Chuan-Ling Si,Zhong-Yuan Li,Ya-Jian Song,Hao Zhou,Tong-Cun Zhang,Xuegang Luo 한국식품과학회 2017 Food Science and Biotechnology Vol.26 No.6
Conjugated linoleic acid (CLA) isomers, c9, t11- CLA and t10, c12-CLA, have been proved to exhibit excellent biomedical properties for potential use in anticancer applications and in reducing obesity. Acer truncatum Bunge (ATB), which is rich in unsaturated fatty acids, including oleic acid, linoleic acid, and nervonic acid, is a new resource for edible oil. In the present study, we developed a new method for producing two CLA isomers from ATB-seed oil by fermentation using Lactobacillus plantarum CGMCC8198 (LP8198), a novel probiotics strain. Polymerase chain reaction results showed that there was a conserved linoleate isomerase (LIase) gene in LP8198, and its transcription could be induced by ATBseed oil. Analyses by gas chromatography–mass spectrometry showed that the concentration of c9, t11-CLA and t10, c12-CLA in ATB-seed oil could be increased by about 9- and 2.25-fold, respectively, after being fermented by LP8198.
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