유전자지문을 이용한 성폭행 피의자의 식별

황적준,나중열 고려대학교 의과대학 1991 고려대 의대 잡지 Vol.28 No.2

Sperms, recovered from vaginal swabs, were processed to isolate high-molecular weight deoxyribonucleic acid(DNA) by the use of a buffer containing sarkosyl and DTT. They were digested with a particular restriction endonuclease, Pst 1. and analyzed with YNH and VI probes that recognize hypervariable regions within the human genomic DNA. The pattern of restriction fragment length polymorphisms(RELPs) with VNTR is identical to that observed with DNA prepared from white blood cells of male sexual partner. Therefore, DNA fingerprint with YNH and VI probe can be used to exclude or to determine the identity of an assailant in rape cases.

Prostaglandin E₂의 子宮內 投與가 家兎의 卵集機能에 미치는 影響에 關한 實驗的 硏究

崔鏡淑,羅重烈,李壽鍾 고려대학교 의과대학 1983 고려대 의대 잡지 Vol.20 No.3

Prostaglandin E₂의 子宮內 投與가 家兎의 卵巢機能에 미치는 影響에 關한 實驗的 硏究

崔鏡淑,羅重烈,李壽鍾 고려대학교 의과대학 1983 고려대 의대 잡지 Vol.20 No.3

The effects of intra-uterine administration of Prostaglandin-E₂on the ovary and plasma steroid hormones were studied. Pseudopregnancy was induced in seven rabbits by the injection of 50㎍ of ethinyl estradiol and 100IU of hCG subcutaneously. And these seven pseudopregnant rabbits served as control group. In the prostaglandin-E₂ was implanted in uterus on Day seven under laparotomy and uterine incision with pentothal anesthesia. The plasma estrogen and progesterone level was determined by radioimmunoassay with three days interval in both control and prostaglandin=E₂ treated group. The uterus and ovaries were submitted to histologic study on Day 30. The following results were obtained; 1. Serum progesterone level in intra-uterine prostaglandin-E₂-implanted pseudopregnant rabbits was decreased at 14th day of pseudopregnancy, while the progesterone level of normal pseudopregnant rabbits was decreased from 15th day of pseudopregnancy and returned to normal values by 18th day. 2. Serum estrogen levels of pseudopregnant rabbit was higher than pretreatment state and returned to normal value by 30th day, while, in prostaglandin-E₂treated group, the serum estrogen values was decreased at 11th and 18th day below the level of pretreatment state and it was more decreased at 28th day. 3. The corpus luteum regresses from 15th day in control group, but in prostaglandin-E₂ Treated group, the corpus luteum was indistinct. The numbers of follicles in control group were 4-5 in number, while, in prostaglandin-E₂treated group, they were increased to 7-11 in number. The endometrium was papillary secretory patterns in control group, while, in prostaglandin-E₂ treated group, the endometrium was shown to have proliferative patterns.

Indomethacin 子宮內 揷入이 卵巢와 血漿 Hormone에 미치는 影響에 關한 實驗的 硏究

朴俊相,蘿重烈,李壽鍾 고려대학교 의과대학 1983 고려대 의대 잡지 Vol.20 No.3

The effects of intrauterine administration of indomethacin on the ovary and plasma steroid hormones were studied. Pseudopregnancy was induced in seven rabbit by the injection of 50 ug of ethinyl estradiol and 100 IU of hCG subcutaneously. And these seven pseudopregnant rabbits served as control group. In the indomethacin-treated group, pseudopregnancy was induced with 50 ug of ethinyl estradiol and 100 IU of hCG, and indomethacin was implanted in uterus on Day seven under laparotomy and uterine incision with pentothal anesthesia. The plasma estrogen and progesterone level was determined by radioimmunoassay with about three days interval in both control and indomethacin-treated group. The uterus and ovaries were submitted to histologic study on Day 30. The following results were obtained; 1. Serum progesterone level in pseudopregnant rabbis was decreased from 15th day of pseudopregnancy and returned to normal values by 18th day, while, in indomethacin-treated group, the high level of serum progesterone was maintained by 7 more days then control group. 2. Serum estrogen levels of pseudopregnant rabbit was higher than pretreatment state and returned to normal value by 30th day, while, in indomethacin-treated group, the serum estrogen values was higher than control group and did not return to normal value by 30th day. 3. The corpus luteum regresses from 15th day in control group, but in indomethacin-treated group, the corpus luteum was maintained till 25th day. The follicles are 4 to 5 in number in control group and 9 to 15 in indomethacin-treated group. The endometrium was papillary growth pattern in control group, but it was functionless in indomethacin-treated group. It seemed that the prostaglandin from the endometrium acts on corporal regression, but it's action on the follicles was different from that on corpus luteum.

子宮摘出이 家兎 卵巢의 機能에 미치는 影響에 關한 實驗的 硏究

金善行,羅重烈,李壽鍾 고려대학교 의과대학 1983 고려대 의대 잡지 Vol.20 No.3

To understand effects of hysterectomy on the retained ovarian function in the rabbits, pseudopregnancy was induced in seven rabbits, control group, by injection of 50㎍ of ethinyl estradiol and 100 IU of hCG subcutaneously. And a week after hysterectomy of another seven rabbits, hysterectomized group, pseudopregnancy was induced with 50㎍ of ethinyl estradiol and 100 IU of hCG. The serum levels of progesterone and estrogen were determined every 3 day by radioimmunoassay and microscopic examination of the ovaries was performed in each group. Both findings in each group was compared and obtained following results. 1. Luteal phase in hysterectomized group was prolonged about 6 days compared to control group. 2. Histologic examination of ovaries revealed functional corpora lutea in hysterectomized group up to 30 days of experiment. 3. Serum estradiol level and histological picture of the ovarian follicles showed no significant differences between two groups during experiment. 4. These findings show that experimental hysterectomy has inhibitory effect on regressive change of the corpora lutea but has no effect on ovarian follicle. These results suggest that PG is the luteolytic hormone in the rabbit. But, it was presumely concluded that the result in estrogen showed which PGF₂α or some other substances in the uterus might be related to the mechanism of folliculogenesis in ovary according to references.

산과 출혈과 수혈(Ⅰ)

구병삼,나중열 한국의학사 1990 診斷과治療 Vol.10 No.1

흰쥐 자궁에 유도된 염증으로 인한 substance P와 CGRP에 대한 면역반응의 변화

나중열(Joong Yol Na),장기훈(Ki Hoon Chang),유상욱(Sang Wook You),홍순철(Soon Choul Hong),이서은(Seo Eun Lee),한희철(Hee Chul Han) 대한산부인과학회 2001 Obstetrics & Gynecology Science Vol.44 No.5

N/A Objective : Recently some reports suggested substance P and CGRP might be important factors for inflammation and hyperalgesia. This study was performed to see whether substance P or CGRP containing nerve fibers might be changed by mustard oil-induced inflammation. Methods : After injection of mustard oil(5%) into uterine lumen, the uteri were removed and examined with immunohistochemical methods for substance P and CGRP. Results : In the normal uterus, most of the substance P- or CGRP-immunoreactive nerve fibers were observed along the vascular structure and some in the myometrium, only few in the endometrium. Mustard oil did not changed this pattern of nerve fiber distribution but after 48 hrs, the amount of substance P or CGRP immunoreactive nerve fibers were greatly reduced compared with the normal uterus. It is not clear whether the decrease of substance P and CGRP immunoreactive fibers in the uterus was resulted from the depletion of the neuropeptides in the nerve fibers or the retraction of nerve fibers. Conclusions : These results suggest that the inflammation should cause the change of nerve fibers included in the nociception. This change may attribute the generation of inflammation and inflammatory hyperalgesia.

Substance P에 의한 자궁의 기계적 수용체의 활동 변화에 대한 전기 생리학적 고찰

나중열(Joong Yol Na),장기훈(Ki Hoon Chang),신재철(Jae Chul Shin),유상욱(Sang Wook You),홍순철(Soon Choul Hong),이서은(Seo Eun Lee),한희철(Hee Chul Han) 대한산부인과학회 2001 Obstetrics & Gynecology Science Vol.44 No.4

N/A Objective : In inflammation, hyperalgesia is a common phenomenon but its mechanism has not been clarified. Recently some reports suggested substance P might be important factors for inflammatory hyperalgesia in somatic tissue. This study was performed to see whether substance P modulate the activities of uterine afferent fibers in the hypogastric nerve of the cat. Methods : While recording the electrical activities of nerve fibers, mechanical stimuli were applied as balloon distention using balloon inserted into uterine lumen before and during substance P infusion through uterine artery. Results : Substance P increased the responses to balloon distension of uterus in 14 uterine mechanoreceptive afferent fibers of 24 over 10% compared to before substance P infusion, and decreased the responses of 3. And L-703,606, the neurokinin 1 receptor antagonist failed the modulation of mechanosensitive response by substance P and reduced the spontaneous activities. Conclusions : These results suggest that substance P modulated the activities of uterine nerve fibers and their responses to mechanical stimulus. It is hypothesized that this kind of modulation of afferent nerve fibers by substance P may be important for the development of inflammatory hyperalgesia.

의과대학 학생현황에 대한 고찰

나중열(Joong Yol Na),최삼섭(Sam Sup Choi),최보율(Bo Yul Choi),정명현(Myung Hyun Chung) 한국의학교육학회 1994 Korean journal of medical education Vol.5 No.2

家兎子宮, 腦 및 胃組織의 Catecholamine 含量과 Human Chorionic Gonadotropin 및 Chlorpromazine이 이에 미치는 影響

羅重烈 고려대학교 의과대학 1977 고려대 의대 잡지 Vol.14 No.1

Chlorpromazine, one of the major tranquilizer, has been used for hypertensive disorders of pregnancy and psychiatric disorders. The drug may induce a number of untoward side-effects, such as amenorrhea, galactorrhea and inhibition of ovulation. Also the drug exerts an action on the central various system in various aspects. An attempt was made to clarify the effects of chlorpromazine on the catecholamine contents of uterine, brain and stomach tissue of mature female albino rabbits weighing approximately 2.0㎏. A group of albino rabbits was conditioned with 50 I.U./㎏ of HCG (human chorionic gonadotropin) for 5 days. Another group was conditioned with 25㎎/㎏ Of chlorpromazine plus 50 I.U/㎏ of HCG for 5 days and observed for 24 hours. Catecholamine content was determined by the Shore and Olin method. The results obtained were as follows: 1. The catecholamine content in normal uterine tissue was 0.40㎍/gm, while that of brain tissue was 0.11㎍/gm and that of stomach tissue 0.16㎍/gm. The catecholamine content in uterine tissue was richest: then in the stomach and in the brain tissue, respectively. 2. In the HCG conditioned group, the catecholamine content in the uterine tissue was 0.13㎍/mg, while that of brain tissue was 0.11㎍/mg and that of stomach tissue was 0.13㎍/mg. It showed that the catecholamine contents in the uterine and stomach tissue were same, and there showed no changes in the brain tissue. 3. In the chlorpromazine plus HCG conditioned group, the catecholamine content in the uterine tissue was 0.15㎍/mg, while that of brain tissue was 0.11㎍/gm and that of stomach tissue was 0.10㎍/gm. It also showed that the catecholamine content of the uterine tissue was richest among the three organs. 4. The catecholamine content of the uterine tissue showed a marked decrease in the HCG conditioned group, while there was lesser degree of decrease in the content of the chlorpromazine plus HCG conditioned group. 5. The catecholamine content of the brain tissue showed no change in HCG conditioned group and in the chlorpromazine plus HCG conditioned group. 6. The catecholamine content of the stomach tissue was decreased by HCG conditioning and it was decreased even more in chlorpromazine plus HCG conditioning. 7. The catecholamine conent of the uterine tissue was markedly decreased in the HCG conditioned group, while there was lesser degree of decrease in the chlorpromazine plus HCG conditioned group. In the brain tissue, the catecholamine content showed no significant change in HCG conditioned group as well as in chlorpromazine plus HCG conditioned group. In the stomach tissue, the catecholamine content showed some decrease in HCG conditioned group and it was decreased more in chlorpromazine plus HCG conditioned group.