DJ-1 deficiency impairs synaptic vesicle endocytosis and reavailability at nerve terminals

Kyung, Jae Won,Kim, Jin-Mo,Lee, Wongyoung,Ha, Tae-Young,Cha, Seon-Heui,Chung, Kyung-Hwun,Choi, Dong-Joo,Jou, Ilo,Song, Woo Keun,Joe, Eun-Hye,Kim, Sung Hyun,Park, Sang Myun National Academy of Sciences 2018 Proceedings of the National Academy of Sciences Vol.115 No.7

<P>Mutations in DJ-1 (PARK7) are a known cause of early-onset autosomal recessive Parkinson's disease (PD). Accumulating evidence indicates that abnormalities of synaptic vesicle trafficking underlie the pathophysiological mechanism of PD. In the present study, we explored whether DJ-1 is involved in CNS synaptic function. DJ-1 deficiency impaired synaptic vesicle endocytosis and reavailability without inducing structural alterations in synapses. Familial mutants of DJ-1 (M26I, E64D, and L166P) were unable to rescue defective endocytosis of synaptic vesicles, whereas WT DJ-1 expression completely restored endocytic function in DJ-1 KO neurons. The defective synaptic endocytosis shown in DJ-1 KO neurons may be attributable to alterations in membrane cholesterol level. Thus, DJ-1 appears essential for synaptic vesicle endocytosis and reavailability, and impairment of this function by familial mutants of DJ-1 may be related to the pathogenesis of PD.</P>

공무원들의 인식에 나타난 경관자원 특성 분석 연구

박선명(Park Sun-Myung),박선경(Park Sun-Kyung),김주현(Kim Joo-Hyun),김종하(Kim Jong-Ha),이정호(Lee Jung-Ho),최무혁(Choi Moo-Hyuck) 대한건축학회 2010 대한건축학회논문집 Vol.26 No.11

In this study, to set up landscaping plans, characteristics of landscape were extracted based on opportunity and weaknesses factors of landscape after carrying out a questionnaire targeting officers in 23 cities and counties in Kyung-buk province and the findings are as following. First, in analyzing condition, component and value of landscape based on opportunity and weaknesses factors, for opportunity factors, it was found that the condition of landscape was quantitatively great, the component of landscape was industrially great and the value of landscape was great in possibility of development. Also, for weaknesses factors, the 'quality' was superior in its condition, the industry was superior in its 'component' and the shortage of artificial resources was superior in its 'value'. Secondly, in the relation between factors consisting opportunity and weaknesses resources, firstly, it was found that industry, city and nature are superior for opportunity factors, and culture, traffic and population are superior for weaknesses factors. Next, in the relation between component and condition, 'qualitatively superior industry' and 'quantitatively superior industry' had the highest frequencies in opportunity factors and 'qualitatively outstanding city' and 'quantitatively preponderant industry' had the highest frequencies in weaknesses factors. Thirdly, in analyzing the importance of opportunity and weaknesses factors, for opportunity factors, qualitatively superior resource was a priority in the importance of condition and for weaknesses factors, it was qualitatively little greater than the quantity. In the importance of component, the industry was most superior in both of strength and weak points and in the importance of value, the shortage of artificial resources was a priority. Fourthly, in characteristics of landscape, internal characteristics were found stronger than external characteristics in each city and county. This means that there are more resources to strategize in landscape plans of individual city and county unit than other in metropolitan unit. Also, possibility of development, possession of complex resources and geographical adjoin were common points of their strategies and plans.

식품 기준·규격의 합리적 관리 ·운영에 관한 연구(Ⅰ) : 두부,식용유지, 전분의 분말상 원료에 관하여 Powdered marerials of Tofu, Edible oil, Starch

김희연,홍진환,박혜경,한상배,박종석,이은주,이정성,송경희,최은희,최영준,소경아,성영제,이주엽 식품의약품안전청 2000 식품의약품안전청 연보 Vol.4 No.-

본 연구는 분말상 원료(전분, 대두분)의 저장유통시 품질변화를 건전성과 안전성 측면에서 검토하여 식품의 기준 ·규격 설정f.」 합지적으로 반영하고자 하였다. 전분과 패두분의 이물을 검사하고, 2종 대두분(중국산, 미국산)을 ?0일간 IS'c와 30t에 저장하면서 성상, 수분, 산가, 과산화물가, 지방산 조성,아플라톡신 BB의 생성여부를 분석하였다. 중국산 대두분 30'』 저장군에서 50일 이후 곰팡이가 발생한 것 이외에 모든 시료에서 양호한 성상을 나타내었고, 전분과 대두닥 모든 시료에서 이물은 검출되지 않았다. 산가는 중국산과 미국산 시료간에 편차를 보여 증국산 30"C, 50일 저장시료에서 약 10배끙도 증가하였고, 과산화물가는 저장 90일후 15'E 저장군은 약 2.3배, 30'c 저장군은 3배정도 증가하였다. 대두분의 주요 지방산은 linoleic acidf18. 2), oleic acid(18 : 1), palmitic acid(16 : 0)이었으며, 3개월 저장에 따른 지방산 함량의 변화는 미미하였다. 아플라톡신 B₁은 모든 시료에서 검출되지 않았다. The objective of this study was to investigate auality changes of type raw materials(starch, soy flour) during various storage conditions. Starchflour(source of China, U.S.A) were slfred under two temperature(15'C, 30'c) forSensory evaluation, (o.reign material test, moisture content, acid value, peroxide ualue,composition, aflatorin Bi were analyzed. China soy flour(stor·ed 30'f) was contaminated by fungj on 50 days. Except for soy flour contardnated by fungi, sensory characteristics were not changed and foreign mater;als were notdetected. Acid value of China soy flour(stored at 30'C, 90 days) was about 10 times higherlevel before storage and acid value was more influenced by source(China, USA). Peroxide valuewas in proportion with the storage period slightly. The major fatty acid of soy flour waslinoleic acid(18 . 2), an(3 the change of fatty acid composition was not observed. Aflatoxlrl Blwas not detected.

급성기 뇌경색 환자에서 화열 관련 증상과 증후의 변화에 관한 연구

곽승혁,박수경,우수경,이은찬,박주영,정우상,문상관,조기호,조승연,박성욱,고창남,Kwak, Seung-hyuk,Park, Su-kyung,Woo, Su-kyung,Lee, Eun-chan,Park, Joo-young,Jung, Woo-sang,Moon, Sang-kwan,Cho, Ki-ho,Cho, Seung-yeon,Park, Sung-wook,Ko, Ch 대한중풍순환신경학회 2011 대한중풍.순환신경학회지 Vol.12 No.1

Objective : Fire and heat related symptoms and signs are considered common in acute stage of diseases. The purpose of this study is to evaluate the occurrences and changes of fire and heat related symptoms and signs in acute cerebral infarction patients. Method & subjects : 40 acute cerebral infarction patients hospitalized in Oriental medicine hospital, Kyung-Hee University, who had examined and diagnosed 2 or 3 times based on oriental medical diagnosis were selected. We chose 23 as fire and heat related symptoms and signs from 94 diagnostic articles, and we added all those scores together of each patient. We analysed the scores of fire and heat related symptoms and signs as the time passed, and depending on oriental medical diagnosis. Result : In acute cerebral infarction patients of this study, 4 of fire and heat related symptoms and signs were took 1st, 2nd, 4th and 8th places in most changeable 10 articles of total 94 articles. The mean score of fire and heat related symptoms and signs of all patients were decreased significantly over the 3 times of measurements. The 8 patients diagnosed as fire and heat diagnosis at visit1 were samely diagnosed as fire and heat diagnosis at visit2, and at visit3 5 patients of them except for 3 patients excluded between visit2 and visit3, were still diagnosed as fire and heat diagnosis. At all of 3 measuring times, the scores of fire and heat related symptoms and signs of fire and heat diagnosis group were higher than non-fire and heat diagnosis group. Conclusion : This study indicated that fire and heat related symptoms and signs were very changeable phenomenon in acute cerebral infarction patients. And they decreased as time goes on.

시멘트 콘크리트의 건조수축균열 특성

박제선,윤경구,이주형,김태환,정원경 강원대학교 부설 석재복합신소재 제품연구센터 2000 석재연 논문집 Vol.5 No.-

콘크리트는 본질적으로 인장강고와 휨강도가 약하며 취성적 성질을 나타내는 결점을 가지고 있으므로 이를 보강할 목적으로 콘크리트에 섬유를 혼입한 섬유보강 콘크리트가 크게 각광받고 있다. 특히 폴리프로필렌 섬유보강은 모르타르 및 콘크리트가 건조나 동해에 의해 수축될 때 구속에 의해 발생되는 인장응력 및 균열을 제어하고 인성의 증가와 충격, 마모, 피로에 대한 저항성, 내구성을 증대시키는 장점을 가지는 것으로 보고 되고 있다. 1축으로 구속된 건조수축의 구속으로 인한 조강형 콘크리트의 잔류응력을 선형모델을 이용하여 예측할 수 있었고 조강형 콘크리트에 섬유보강으로 인한 건조수축 제어효과는 일반큰코리트에 비해 효과가 매우 큰 것으로 나타났다. 따라서 폴리크로필렌 섬유의 혼입으로 인해 콘크리트의 균열제어가 가능하며, 균열 폭이 큰 균열보다는 많은 미세 균열을 유도하는 것을 알 수 있었다. 섬유보강 조강형 큰크리트의 동결융해저항특성은 거의 동해의 손상을 받지 않으며 좋은 내구성을 유지하고 있는 것으로 나타났다. 이는 불규칙하게 배향된 섬유가 공극수의 이동을 효과적으로 제어함으로써 메트릭스 내의 팽창압으로 인한 파괴를 최대한 줄였기 때문이라고 보여진다. 표면손상과 박리저항에 매우 우수하며 저항성을 증진시키는 효과가 뛰어남을 확인할 수 있었다. 따라서, 내구적 측면의 유지, 보수에 있어서도 섬유보강은 그 효과가 큰 것으로 나타났다. To improve the brittle nature of concrete, discrete fibers can be adapted in concrete. Since polypropylene fiber has many advantages in many points on its chemical stability and good durability, its usages have been increased gradually. It has been reported than polypropylene fibers can not only control restrained tensional stresses and cracks, but also increase toughness, resistance to impact, corrosion, fatigue, and durability. Although the polypropylene fiber reinforced concretes with ordinary cement have been studied so much, those with very early strength concretes have not studied at all. Therefore, it is necessary to initiate and conduct the researches for the polypropylene fiber reinforced very early strength concretes which has enough workability and durability. This study of polypropylene fiber reinforced very early strength concrete focused on the investigation each material's effect into strength development with analysis of restrained shrinkage properties with one-dimensional concrete members. The results showed that more optimized mixture combinations could be selected in very early strength concrete by incorporating polypropylene fibers, keeping in same strength levels at urgent repair works. The formation of needle-shape ettringite helps the early strength development, specially at flexural and this enables the repaired concrete structures or pavements to be opened to traffic after 3 hours of concrete placement. A residual stress of early strength concrete caused by one-dimensional restrained drying shrinkage was predicted from linear model. the control of shrinkage crack by fiber reinforcement at early strength concrete was better than that of ordinary portland cement concrete because of bridging effect of fiber at early age and resulting of more hair cracks rather than fewer wider cracks. As a results, this dissertation clearly validated the fiber reinforcement on the strength development and durability of polypropylene fiber reinforced very early strength concrete, and characteristic of restrained shrinkage. The proposed optimized concrete mixtures might be satisfactory applied at the repair works in fields, because of its durability and crack control capacity against freeze-thaw and contraction at early age, respectively.

CARBAMATE의 새 합성방법에 대하여(제1보)

주완철,박경래 成均館大學校 1979 論文集 Vol.26 No.-

Previous investigators have prepared carbamates by the Hoffmann-like rearrangement of amides using lead tetraacetate as an oxidation agent. Almost of these investigations are concerned mainly with the procedures of the carbamate preparations. For the formation of carbamates by the Hoffmann-like rearrangement of amides it seems very important to know the influences of the character of amides and also the acidity of alcohols. In this work we have studied at first the influences of amides with electron withdrowing and donating character upon the formation of carbamates. From our results we have found that there is no remarkable influence of amides on account of the yield or the reactivity. We have also found that this reaction does not initiate without base. Therefore we have concluded that the formation of isocyanate intermediate in the process of carbamate preparation occurs by a spontaneous intramolecular synchronous rearrangement and not by the free nitrene formation, and that the catalytic effect of a base, which acts to displace a nitrogen bonded proton of the amide and initiates the reaction, must be accounted as an important factor.

연안 도시 대기오염 물질의 농도분포 특성

박종길,석경하,김지형,차주완 한국환경과학회 2002 한국환경과학회지 Vol.11 No.12

This paper aims to find the characteristics of concentration distribution of coastal urban air pollutants. For this purpose, It was used the daily meteorological data and hourly concentration data O_3 and NO_2 in Busan metropolitan city from 1994 to 1996. It was investigated the annual and monthly distribution of ozone and nitrogen dioxide concentration at each site in Busan, and also investigated the characteristics of concentration change of air pollutants with time under the sea breeze. As a results, the concentration of nitrogen dioxide and ozone tend to be increased every year and nitrogen dioxide concentration is higher than ozone concentration at all site in Busan. The concentration of ozone is high in summer season and low in winter season, but the concentration of nitrogen dioxide have a reversed trend. The monthly peak concentration of ozone occurred in April and September, while the monthly minimum concentration of nitrogen dioxide occurred in August. Their trend were indentified by sites near the coastline than sites stands apart from the coastline. The sea breeze occurred annual mean 81 days in Busan from 1994 to 1996. The main wind direction of sea breeze was classified into southeasterly. In case of southwesterly, It was pronounced the south wind and southwest wind. In case of southeasterly, the occurrence frequency of east wind was high. Especially, the concentration of urban air pollutants, such as ozone and nitrogen dioxide, were high on time which the sea breeze flow, and the areas that ozone concentration was high moved from outside part to central part of city with time. In costal urban such as Busan, the wind direction of sea breeze is influenced the influenced the change of ozone and nitrogen dioxide concentration on time which the sea breeze flow at each site and air pollutants concentration of sites on the pathway of sea breeze.

호흡 부전을 동반한 급성 자율-감각신경병증 1예

박경필,안규환,김은주,김대성,정대수,박규현,김매자 대한신경과학회 1999 대한신경과학회지 Vol.17 No.5

장시간 불가피한 공복(fasting)후 인체의 내분비 변화 : 삼풍백화점 붕괴 사고후 구조된 3인의 증례 THREE CASES IN COLLAPSE OF SAMPOONG DEPARTMENT STORE

박규남,황주일,박조현,오동렬,이원재,오승택,김세경,김인철 대한응급의학회 1995 대한응급의학회지 Vol.6 No.2

The interactions between nutrition and the endocrine system are more intimate and complex that previously thought. Nutrition modulates secretion and activity of many hormones, as part of the stress adaptation process. Fasting, in particular, produces a number of important changes in the endocrine system, with teleological purpose of helping the body tolerate the lack of food ingestion. These changes are therefore beneficial and protective, although at times they may not appear to be that way. The endocrine changes of fasting mediate the metabolic response. We must emphasize that these changes are completely reversible when appropriate nutrition is established. These changes are as followes: 1) decreased insulin and increased glucagon levels, 2) decreased thyroid effect, 3) decreased sympathetic activity, 4) hypothalamic hypogonadism, 5) decreased growth, 6) altered glucocorticoid secretion and metabolism, 7) impaired mineralocorticoid response, 8) decreased ADH secretion and effect. We experienced three cases of involuntary prolonged fasting after collapse of Sampoong department store. They were released from collapsed field after 11(case Ⅰ), 13(case Ⅱ), 17days(case Ⅲ) respectively. In each case, the endocrine changes of plasma levels were as followes: decrease of cortisol in case Ⅰ, increase of GH in case Ⅱ, decrease of free T3, T3, cortisol and increase of reverse T3, insulin in case Ⅲ. Each one did not show the same endocrine changes of plasma levels. But we think these changes of fasting are beneficial in metabolic response of human.

건설용 LIFT의 안전사고 예방에 관한 연구

박주식,오종구,강경식 明知大學校 産業技術硏究所 2000 産業技術硏究所論文集 Vol.19 No.-

An aspect of recently happening industrial accident is increasing serious accident than a slight accident. Especially, accident for construction Lift to construction work was showing decreasing tendency annually, was showing increasing tendency form 1995, and was showing still more serious problem than other kind of accident because of a wounded person more than the killed and injured. A study is th object to prevent noble life that industrial accident of producted construction Lift within the country from 1994 to 1998 is survey and analysis.