지능형 에이전트를 사용한 가상 대학 설계

이홍주,이찬도 대전대학교 산업기술연구소 1999 산업기술연구소 論文集 Vol.10 No.2

Agents are a powerful abstraction through which users may interact with a complex data universe by delegating tasks involving objects or events of interest. Digital libraries are one of the many application areas where agent technologies are used. In these architectures, digital libraries are seen as a decentralized collection of interacting self-interested agents where an agent represents the knowledge and interests of an individual user. This paper describes intelligent agents based architectures for digital libraries and suggest an intelligent agents based virtual university, a limited version of digital library.

Preventive Glass lonomer Restoration의 치험증례

이종갑,이주헌,손흥규,최병재 大韓小兒齒科學會 1993 大韓小兒齒科學會誌 Vol.20 No.2

The “preventive glass ionomer restoration ”was first described by Garcia-Godoy in 1986, which was a variation of the preventive resin restoration. The preventive glass ionomer restoration has disadvantages of low wear resistance, technique sensitive, and no long term clinical evaluation. However, it has advantages as mininal setting shrinkage, fluoride release, chemical bonding to dentin and enamel, coefficient of thermal expansion sinilar to that of tooth structure, and low pulpal toxicity. Therefore, applying this technique on low stress bearing area of initial carious lesion can be a good restorative method.

SMAGE1/2 단백질의 생산과 anti-MAGE-1 polyclonal 항체의 생성

이정희,김종진,박주홍 昌原大學校 基礎科學硏究所 1999 基礎科學硏究所論文集 Vol.11 No.-

MAGE-1, with was originally identified by reacting with cytolytic T lymphocytes derived from the blood of melanoma patients, is a member of a gene family consisting of twelve structually and expressionally related genes, called MAGE-1 to 12 located in the Xq28 region. The MAGE genes are expressed only in testis among normal tissues and in a variety of tumors. However, what biological functions it has is currently unknown. SMAGE genes were found in a study aiming at detecting mouse genes homologous to human MAGE genes. Because mice are generally easier than humans to work on in studies endeavored for functional analysis of genes, functional study of SMAGE may be very helpful in understanding biological functions of MAGE protein. In the present study we have tried to develop antibodies against SMAGEl/2 protein to better understand biological functions of SMAGEl/2. We cloned the SMAGE-1 cDNA spanning exon Ⅲ amplified by polymerase chain reaction using mouse hepatocyte genomic DNA into an Escherichia coli expression vector and were able to produce Ni-NTA resin-purified recombinant SMAGEl/2 protein of 43 kDa on SDS/PAGE which was subsequently used for immunizing mice. Sera collected from immunized mice were shown to specifically react with SMAGEl/2 antigen by ELISA in combination with Western blotting This polyclonal anti-SMAGE serum will be used for functional studies of MAGE protein.

고속가공에서 박막 측벽(Thin wall) 파트 가공을 위한 연구

김홍배,이우영,최성주 한국공작기계학회 2000 한국공작기계학회 추계학술대회논문집 Vol.2000 No.-

The term 'High Speed Machining' has been used for many years to describe end milling with small diameter tools at high rotational speeds, typically 10,000 - 100,000 rpm. The process was applied in the aerospace industry for the machining of light alloys, notably aluminium. In recent years, however, the mold and die industry has begun to use the technology for the production of components, including those manufactured from hardened tool steels. And the end-mill is an important tool in the milling process. A typical examples for the end mill is the milling of pocket and slot in which a lot of material is removed from the workpiece. Therefore the proper selection of cutting parameters for end milling is one of the important factors affecting the cutting cost. The one of the advantages of HSM is cutting thin-wall part of light alloy like Al (thinkness about 0.3㎜). In this paper, firstly, we study characteristics of HSM, and then, we choose the optimal parameters(cutting forces) and investigate various machining strategies to cut thin-wall part by experiment.

Protein microarray를 이용한 Apin-단백질의 상호작용에 관한 연구

박주철,박선화,김흥중,박종태,윤성호,김지웅,이태연,손호현 대한치과보존학회 2007 Restorative Dentistry & Endodontics Vol.32 No.5

분편 인플루엔자백신(split influenza vaccine)의 임상효과 및 면역원성에 관한 연구

우흥정,김동림,정희진,천병철,이주연,안정배,김지희,박찬,신영규,김우주,김민자,박승철 대한화학요법학회 1999 대한화학요법학회지 Vol.17 No.1

목적 : 아단위 인플루엔자 백신 접종 후 백신의 인플루엔자 예방효과, 인플루엔자 방어 항체형성, 인플루엔자 백신의 안전성을 조사하고자 하였다. 방법 : 총 571명을 대상으로 인플루엔자 백신 접종을 하였고, 이들 접종자에서 인플루엔자 양질환의 이환을 조사하여 인플루엔자 백신의 인플루엔자예방효과를 알아보았고, 백신의 접종 전 및 접종 4주 후 혈청에서 혈구응집억제물(Hemagglutination Inhibition : HAI) 항체 검사를 실시하여 백신의 방어항체생성을 조사하였고, 백신의 안전성을 알아보기 위해 백신접종 후 1주일 이내의 부작용을 조사하였다. 결과 :백신 접종군과 백신 비접종군에서 인플루엔자 양 질환의 이환을 조사한 결과 접종군 28.35%, 비접종군 35.88%으로 나왔으며, p 값이 0.001로 통계적으로 접종군에서 유의하게 낮았고, 인플루엔자 양 질환의 예방 효과는 20.97%를 보였다. 백신의 방어항체 형성의 평가를 위해 유럽의 인플루엔자 백신 허가 기준을 조사하였는데 B/Guangdong/5/94균주의 백신접종 후 항체가 40이상의 비율을 제외한 다른 기준은 모두 만족 시켰다. 부작용은 전체 조사자 521명중 149명(29%)으로 주로 접종 부위의 국소 부작용을 호소했고, 전신 부작용은 2% 내외였으며 특별히 심각한 부작용은 발견되지 않았다. 결론 : 분편 인플루엔자 백신은 인플루엔자양질환의 예방과 방어항체생성에 효과 있으며 안전한 것으로 사료된다. Background : The safety and effectiveness of influenza vaccine are well known in developed country. The influenza vaccination has been recommended as one of the tentative immunization schedule for indicated persons since 1997 in Korea. But there are still no available data about them, even though nearly 5 million doses of influenza vaccine were used in 1997-1998 season. So it is immediately needed to investigate the safety. efficacy and immunogenicity of influenza vaccine among Korean. Methods : We studied the clinical efficacy of influenza vaccine by monitoring Occurrence of influenza-like illness in influenza risk group(vaccination ; 300, non-vaccination; 215) from December in 1997 to March in 1998. We used the split quadrivalent influenza vaccine containing 15 microgram of hemagglutinin of A/Beijing/262/95(HlNl), A/Wuhan/359/95(H3N2), B/Mie/1/93 and B/Guangdong/5/94. Hemagglutination inhibition(HA1) antibody titers were determined before immunization and 1 months after vaccination And we evaluated adverse effect of influenza vaccination at 7 days after vaccination. Results : Influenza vaccination was associated with si@icant reductions in influenza-like spptoms(vaccination group; 28.35%, non-vaccination group, 35.88%, p=0.001). The preventive effect of influenza-like i3lne.s among influenza risk goup was 20.97%. And immunogenicity of influenza A and B exceeded all of the European licensure criteria for immunogenicity except postvaccination proportion of titers 240 of B/Guangdong/5/94 strain. And the adverse effects were mainly local injection site problem and no serious adverse effect was noted. Conclusion : Split influenza vaccine is safe, inmunogenic and eff'tive in influenza risk group in Korea.

적외선 열화상 카메라를 이용한 고속가공에서의 열 발생 특성

김흥배,이우영,최성주,유중학 한국공작기계학회 2001 한국공작기계학회 춘계학술대회논문집 Vol.2001 No.-

The term 'High Speed Machining' has been used for many years to describe end milling with small diameter tools at high rotational speeds, typically 10,000 - 100,000 rpm. The process was applied in the aerospace industry for the machining of light alloys, notably aluminium. In recent year, however, the mold and die industry has begun to use the technology for the production of components, including those manufactured from hardened tool steels. With increasing cutting speed used in modern machining operation, the thermal aspects of cutting become more and more important. It not only directly influences in rate of tool wear, but also will affect machining precision recognized as thermal expansion and the roughness of the surface finish. Hence, one needs to accurately evaluate the rate of cutting heat generation and temperature distributions on the machining surface. To overcome the heat generation, we used to cutting fluid. Cutting fluid play a roles in metal cutting process. Mechanically coupled effectiveness of cutting fluids affect to friction coefficient at tool-work-piece interface and cutting temperature and chip control, surface finish, tool wear and form accuracy. Through this study, we examined the behavior of heat generation in high-speed machining and the cooling performance of various cooling methods.

치수제거후 흰쥐 삼차신경절에서 VIP 면역반응세포의 변화: 공초점레이저주사현미경적 연구

김흥중,김승재,박주철,박주철,이상호 대한소아치과학회 2001 大韓小兒齒科學會誌 Vol.28 No.1

말초신경 손상에 의한 VIP의 변화를 연구하기 위해 흰쥐 하악대구치 치수제거 후 삼차신경 절에서 VIP의 분포 및 반응강도를 공초점레이저주사현미경을 이용하여 관찰하였다. 체중 200g내외의 Sprague-Dawley계 흰쥐를 대조군과 하악대구치 치수제거 후 14일군으로 분리하여 희생시켰다. 1차 항체로 rabbit anti-VIP, 2차 항체로 fluorescein isothiocyanate(FITC) conjugated anti-rabbit IgG를 사용하여 면역형광염색을 시행한 후 공초점레이저주사현미경으로 관찰하여 다음과 같은 결론을 얻었다. 1.삼차신경절 하악부위에서 VIP 양성반응세포의 비율은 대조군에서 7.40%를, 실험군에서는 28.42%를 보였다. 대조군에 비해 실험군에서 양성반응세포의 증가를 보였다. 2.삼차신경절 하악부위에서 VIP 면역반응세포체에 대한 상대성 형광강도는 대조군에서 87.78을, 실험군에서는 138.65를 보였다. 대조군과 비교하였을 때 실험군에서 상대성 형광강도의 증가를 보였다. 3.실험군의 광연속절편(1㎛) 관찰에서 VIP 면역반응세포는 9개의 절편 대부분에서 강하게 나타났다. 축삭의 면역반응을 살펴보면, 대조군의 축삭에서는 약한 반응을 보였으며, 실험군의 축삭에서는 강한 면역반응을 보였다. 또한 양성반응 세포체의 크기는 20∼25㎛의 중간 크기의 세포체에서 강한 면역반응을 보였다. 위의 결과로 보아 치수제거 후에 삼차신경절 하악부위에서 VIP면역반응세포의 증가와 함께 상대성 형광강도가 높아졌음을 알 수 있었다. The purpose of this study was to investigate the distribution and fluorescence intensity of vasoactive intestinal polypeptide immunoreactive(VIP-IR) cells in rat trigeminal ganglion following pulp extirpation of rat mandibular molar. The animals were divided into control group(n=6) and experimental group(n=6). The experimental animals were sacrificed at 14 days after pulp extirpation. The trigeminal ganglion was removed and immersed in the 4% paraformaldehyde in 0.1M phosphate buffer. Serial frozen sections about 20㎛ in thickness were cut with a cryostat The immunofluorescence staining was performed. The rabbit anti-VIP(1:8,000) was used as primary antibody and fluorescene isothiocynate(FITC) cojugated anti-rabbit IgG(1:80) as secondary antibody. The slides were observed under confocal laser scanning microscope(CLSM). Unprocessed optical sections were obtained and stored on a optical disk. Color pictures were printed by a video copy processor. The results were as follows ; 1. The positive ratio of VIP-IR cells in mandibular part of trigeminal ganglion were 7.40% in control group and 28.42% in experimental group(14 days affter pulp extirpation). 2. The relative fluorescence intensity of VIP-IR cells in mandibular part of trigeminal ganglion were 87.78 in control group and 138.65 in experimental group. The relative fluorescence intensity of experimental group was 58% higher than that of control group. 3. In optical serial section analysis of VIP-IR cells of experimental group, most of the 9 section showed high fluorescence intensity. At high magnification, axons of the experimental group displayed greater VIP-IR than in the control group, and the positive cells were mainly of medium size. The result indicate that number and fluorescence intensity of VTP-IR cells were increased in the mandibular part of trigeminal ganglion following pulp extirpation of mandibular molar, and it suggests that VIP could play a role in processing of nociception.

NFI-C 결손 생쥐의 상아모세포 분화과정에서 DSPP와 BSP mRNA의 발현

정현구,이창섭,,이상호,이난영,김홍중,박주철 大韓小兒齒科學會 2005 大韓小兒齒科學會誌 Vol.32 No.4

NFI-C는 정상적으로 상아모세포, 골모세포에 존재하며,NFI-C가 결손된 경우 치근상아질을 형성하는 상아모세포의 분화에 이상이 있다고 알려져있다. 본 연구는 NFI-C(-/-) 생쥐에서 치관 및 치근의 상아질을 형성하는 상아모세포의 표현형을 상아모세포에 특이적으로 발현하는 DSPP와 골모세포에 특이적으로 반응하는 BSP 유전자를 이용한 in-situ hybridization을 통하여 연구하여 다음과 같은 결과를 얻었다. 1.NFI-C(-/-) 생쥐의 구치에서 치관 상아질은 정상적으로 형성되었으나, 치근 상아질은 형성되지 않았다. 2.NFI-C(-/-) 생쥐의 하악 절치의 순측 상아질은 비교적 많이 형성되었으나, 설측의 상아질은 형성되지 않았다. 3.NFI-C (-/-) 생쥐의 하악 절치의 상아모세포는 형태가 변화되었으며, 형성된 상아질 내에 세포가 함입되는 양상을 보였다. 4.NFI-C (-/-)생주의 구치에서 치관부위의 상아모세포에서는 DSPP가 강하게 발현되었으나. 치근부위의 상아모세포에서는 발현되지 않았다. 또한 NFI-C(-/-) 생쥐에서 절치의 순측 상아모세포에서는 DSPP가 약하게 발현되었다. 5. 정상 생쥐에서 절치의 상아모세포에서는 BSP가 발현되지 않았으나. NFI-C (-/-) 생쥐에서는 BSP가 강하게 발현되었다. 이상의 결과를 종합하여 볼 때 NFI-C가 결손된 경우 상아모세포가 골모세포로 분화되는 표현형의 변화를 보이는 것으로 사료된다. Nuclear factor I (NFI) exists in the odontoblast and osteoblast.NFI-C null mice demonstrated aberrant odontoblast differentiation, abnormal dentin formation, and molar lacking roots. The purpose of this study was to examine phenotype of the aberrant odontoblast in NFI-C null mice and to evaluate the expression of DSPP and BSP mRNAs in NFI-C null mice with in-situ hybridization. The results were as follows: 1.In the NFI-C (-/-) mice, the crown dentin of molar showed normally formation, but there was no root dentin. 2.In the NFI-C (-/-) mice, the labial dentin of mandibular incisors showed relatively a lot of dentin formation, but the lingual dentin showed defect. 3.In the NFI-C (-/-) mice, the odontoblast of mandibular incisors revealed abnormal shape and trapped in osteodentin-like mineralized tissue. 4.In the NFI-C (-/-) mice, the odontoblast in the crown dentin of molars showed strong expression of DSPP, the odontoblast in the root dentin of molars was not expression of DSPP. In the NFI-C (-/-) mice the odontoblast in the mandibular incisors showed weekly expression of DSPP. 5.In the wild mice, the odontoblasts of mandibular incisors were not expression of BSP, but in the NFI-C (-/-) mice the odontoblast of mandibular incisors showed strong expression of BSP. These results suggest that odontoblst in the NFI-C (-/-) mice changes the phenotype into osteoblast.

Nuclear Factor I-C 결손 생쥐에서 상아모세포의 형태학적 특징

고승백,이창섭,이난영,이상호,김흥중,박주철 大韓小兒齒科學會 2006 大韓小兒齒科學會誌 Vol.33 No.2

NFI-C K/O 생쥐에서는 상아모세포의 분화과정에 이상이 초래되어 상아질 형성에 이상이 생기고 치근 형성이 불완전하게 이루어지는 것으로 알려져 있으나 이에 대한 명확한 기전은 잘 알려져 있지 않다. 상아모세포가 분화하여 정상적으로 상아질을 형성하기 위해서 핵과 세포질이 극성을 띠고 잘 조직화되어야 하며, 이 과정에서 다양한 세포사이 결합장치들이 중요한 역할을 하는 것으로 알려져 있다. 본 연구에서는 NFI-C K/O 생쥐에서 상아질 형성에 이상이 생기는 것이 상아포세포의 형태학적 변화와 세포사이 결합장치들이 기능을 하지 못한 결과에서 기인한 것인지 알아보기 위하여, NFI-C K/O 생쥐에서 발생한 비정상적인 상아모세포들을 광학 및 투과 전자현미경을 이용하여 형태학적으로 관찰하고, Zo-1과 occludin의 발현을 면역조직화학적으로 관찰하여 세포사이 결합장치들의 분포를 확인하여 다음과 같은 결과를 얻었다. 1. 광학현미경 소견에서 NFI-C K/O 생쥐의 전치부 상아모세포는 세포 극성이 상실되고, 여러 층으로 배열되어 있었으며 상아질에 많은 세포들이 함입된 것과 같은 비정상적인 상아질의 소견을 나타냈다. 반면에 NFI-C K/O 생쥐의 구치부 상아모세포는 치관부에서는 잘 조직화된 소견을 보였으나 치근 형성 부위에서는 세포 배열이 불규칙해지고 세포 극성이 상실되었다. 2. 투과 전자현미경 소견에서 NFI-C K/O 생쥐 전치부의 비정상적인 상아모세포는 둥근 형태로 세포 사이 간격이 넓으며 폐쇄연접과 같은 세포사이 결합장치들이 전혀 관찰되지 않았다. 3. Zo-1의 면역조직화학적 염색에서 NFI-C K/O 생쥐의 전치부 법랑모세포의 근위부와 원위부에서 ZO-1이 강하게 발현되었으나 비정상적인 상아모세포에서는 ZO-1의 발현을 관찰할 수 없었다. 4. Occludin의 면역조직화학적 염색에서 정상 생쥐의 전치부 상아모세포에서는 occludin의 발현이 관찰되었으나 NFI-C K/O 생쥐의 비정상적인 상아모세포에서는 occludin의 발현이 관찰되지 않았다. 이상의 결과를 종합하여 볼 때 NFI-C의 결손은 상아모세포의 분화 이상을 초래하고 비정상적으로 상아질을 형성하는 과정에 세포사이 결합장치의 상실과 같은 형태학적인 변화의 중요한 요소로 작용하는 것으로 생각된다. NFO-C null mice demonstrated aberrant odntoblast differentiation and thus abnormal dentin formation while other tissues/organs in the body, including ameloblasts, appear to be unaffected and normal. However, little is known about the mechanism of NFI-C function in odontoblast differentiation and dentin formation. Odontoblasts are tall, highly polarized cells that are responsible for formation and maintenance of the predentin and dentin. An indication of their polarity is the acquisition of specialized intercellular junctions. As predontoblasts differentiate into odontoblasts, they are joined and attached at the apical end by well developed terminal webs of cytoskeletal actins, and associated tight as well as adherent njunctions. In this study, in order to investigate if disruption of the NFI-C gene interferes with formation of a specific or other structural proteins of the intercellular junctions, we examined morphological charcteristic of the aberrant odontoblast in NFI-C null mice using light and electron microscope. In addition, we determined the expression of major structural proteins of intercellular junctions, ZO-1 and occludin, during the differentiation of odontoblasts using immunohitochemistry. The results were as follows: 1. In light microscopy, abnormal odontoblasts of incisors of the NFI-C null mice were round in shape, lost their polarity, and trapped in osteodentin-like mineralized tissue. Mutant molars have relatively normal crowns, but short and abnormal differentiating adontoblasts in root formation area. 2. Electron microscopy of abnormal odontoblasts revealed the dissociation of the round osteoblast-like cells, the loss of their cellular polarity, and the absence of an intercellular junctional complex known as the tight junctions. 3. A mutant incisor showed labeling for ZO-1 at the proximal and distal ends of secreting ameloblasts, while staining for ZO-1 was not observed in the abnormal odontoblasts. 4. A normal incisor showed immunoreactivity for occludin in the differentiating odontoblasts. However, staining for occludin was not observed in the abnormal odntoblasts of mutant incisor. These results suggest that NFI-C gene causes dissociation of odontoblast and thus abberant odontoblast differentiation and abnormal dentin formation by interfering with the formation of intercellular junctions.