방사선 조사 후 대뇌결질에 분포한 Neuropeptide-Y 양성신경세포에 관한 면역조직화학적 연구

이상욱,김종중,정윤영,정종달,오윤경 조선대학교 2000 The Medical Journal of Chosun University Vol.25 No.2

Background and Objectives : Severe irradiation on the cerebral cortex of the human and animals may result in functional alterations of central nervous system. The purpose of this study, the irradiation effects on the cerebral cortex of the rats after brain irradiation was to investigate the change of distribution and morphology of neuropeptide-Y(NPY) neurons. Materials and Methods : Radiation was produced by the linear accelerator 6MV X-ray, and the animals were categorized into control and experimental groups and we use 45 Sprague-Dawley rats weighing about 200~250gm. The head areas of the animals were positioned within the radiation field of 12㎝ × 20㎝ and with the radiation depth of 1.5㎝. Sodium chloral hydrate-anesthetized rats were exposed to the radiation with the dose rate of 240 cGy/min. The total dose was 1800 cGy. Animals were sacrificed on 2 hours, 5 hours, 1 day, 3 days, 7days after brain irradiation. Under anesthesia, animals were perfused with 4% paraformaldehyde-1% glutaraldehyde solution. On one or two hours after the perfusion, brain were taken out and refixed over night in the same fixative. Using ABC immunohistochemistry, morphology and distribution of neuropeptide-Y immunoractive(NPY-IR) neurons were studied on the cerebral cortex of the control and brain-irradiated rats. We used light, transmission electron and confocal laser scanning microscope. Results : The following results were obtained : 1. On control group, NPY-IR neurons were found in all layers of the primary sensory and motor cerebral cortex, and the NPY-IR neurons were concentrated within the layer II, III, IV, V and VI. The typical NPY-IR perikarya was bipolar and multipolar shape. 2. On 2 hours, 5 hours, 1 day after X-irradiation, decreased number of NPY-IR neurons were detected in the primary sensory and motor cerebral cortex of the rats. Also shrunken and transformed NPY-IR neurons were detected in the primary sensory and motor cerebral cortex of the rats. 3. On 3 days and 7 days after X-irradiation, morphology and distribution of NPY-IR neurons in the primary sensory and motor cerebral cortex was generally restored. 4. In optical serial section analysis of NPY-IR neurons, high fluorescence intensity were observed in a part of the 8~11 sections of the control and all irradiated groups. In optical single section analysis of NPY-IR neurons, red color(high fluorescence intensity) was observed in a part of 6, 7 sections of the control and all irradiated groups. 5. By electron microscopy, NPY-IR neurons on 2 hours, 5 hours, 1 day after X-irradiation rats exhibited severe alterations of their organelles concerning intracellular material transport ; such as disappearance of microvilli and basal infoldings, reduction of invaginating pits on the basal and apical plasma membranes, reduction of transformed vesicles and shrunken Golgi complexes, etc. Conclusion : From the above results, it was concluded that the release of neurotransmitters and transcapillary leakage of blood substance were occurred on 2 hours, 5 hours, 1 day after X-irradiation, but the condition was generally restored on 3 days and 7 days following X-irradiation.

현행 중등학교 과학 실험 , 실습 교육 실태 조사 및 그 운영 진단 (Ⅱ) : 고등학교 과학 실험 , 실습 교육을 중심으로

김영호,이윤종,정원우,강용희,양승영,윤성효,안병호,윤일희,권용주,임성규,오철한,기우항,전명남,김중욱 한국과학교육학회 1998 한국과학교육학회지 Vol.18 No.3

본 연구는 96년도에 실시한 우리 나라 중학교 과학 실험·실습 교육의 실태 조사 연구에 따른 계속 연구의 일환으로 고등학교 과학 실험·실습 교육의 실태를 조사하기 위한 것이다. 연구내용은 고등학교 과학 교과서의 분석, 실험·실습 실시 현황 분석, 현행 실험·실습 교육의 문제점 분석, 현행 학교 구성원들의 실험·실습 교육 운영 실태 조사 및 현행 실험·실습 교육의 개선을 위한 연구과제 도출을 주요 연구내용으로 다루었다. 연구방법은 전국의 고등학교 80개교를 대상으로 이들 학교의 학생 1,977명, 교사 165명, 학교장 80명에게 적용하였다. 본 연구에서 밝혀진 결과를 중심으로 우리 나라 고등학교 과학 실험 실습 교육의 정상화를 위한 실험 내적인 연구과제를 제시하면 다음과 같다. (1)교재 개발에 대한 연구, (2)실험 킷트 개발에 대한 연구, (3)교사교육 및 재교육 프로그램 개발에 대한 연구, (4)교실 밖 실험·실습 활동 프로그램 개발에 대한 연구, (5)과학 실험·실습 소재의 개발에 대한 연구. This study was accomplished to analysis and survey on the experimental and practical science education of high school in korea for the consecutive study of the an analysis and survey on the experimental and practical science education of middle school in korea(Lee, Yoon-Jong et al., 1997). The status of facilities, management for the experiment, practices, teaching methods in high schools have been investigated. The present status and reasonable management of the high school science education have been grasped from the questionaires. To do this 165 high school science teachers, 1977 students and 80 principals of high schools in Korea are administered questionaires of Science Education Research Institute of Kyungpook National University(1997). The results of this study are as follows : The reasonable management for experiments and practices of science education were scanty in the high school around the urban and rural school owing to the shortage of facilities and equipments, crowded class, excessive class works for teacher, excessive contents of present textbooks and insufficiency of the administrative supports etc. The current teaching method of high school science has emphasized knowledge. This fact does not satisfy the objective of learning due to lack of the teaching method. Desirable directions for the improvement of present status of high school science education were proposed in this paper.

정신분열병에 대한 리스페리돈의 효과 및 안정성

이민수,김용구,김영훈,연병길,오병훈,윤도준,윤진상,이철,정희연,강병조,김광수,김동언,김명정,김상훈,김희철,나철,노승호,민경준,박기창,박두병,백기청,백인호,손봉기,손진욱,양병환,양창국,우행원,이정호,이종범,이홍식,임기영,전태연,정영조,정영철,정인과,정인원,지익성,채정호,한상익,한선호,한진희,서광윤 大韓神經精神醫學會 1998 신경정신의학 Vol.37 No.1

연구목적 : 본 시험의 목적은 임상시험 시작전에 연구자들을 대상으로 PANSS Workshop을 통하여 PANSS, ESRS에 대한 국내에서의 표준화 작업을 구축하고 새로운 정신병 치료제인 리스페리돈의 효과와 안정성을 재확인하여 리스페리돈 사용에 대한 적정화를 이루는데 있다. 연구방법 : 1996년 4월부터 1996년 9월까지 국내 39개 대학병원 정신과에 입원중인 혹은 증상이 악화되어 입원하는 정신분열병 환자 377명을 대상으로 다시설 개방 연구를 시행하였다. 1주일간의 약물 배설기간을 가진후, 리스페리돈을 8주간 투여하였고, 기준점, 1주, 2주, 4주, 그리고 8주후에 평가되었다. 용량은 제1일에는 리스페리돈 1mg씩 1일 2회, 제2일에는 2mg씩 1일 2회, 제3∼7일에는 3mg씩 1일 2회 투여하였다. 이후 환자의 임상상태에 따라 임의로 증량할 수 있으며, 최대 일일 16mg을 초과하지 않도록 하였다. 추체외로 증상을 조절하기 위한 투약을 허용하였다. 임상증상 및 부작용의 평가는 PANSS(Positive and Negative Syndrome Scale), CGI(Clinical Global Impression) 그리고 ESRS(Extrapyramidal Symptom Rating Scale)을 사용하였다. 연구결과 : 377명중 343명(91%)이 8주간의 연구를 완결하였다. 치료 종결시점인 8주후 PANSS 총점수가 20% 이상 호전된 경우를 약물 반응군으로 정의할때, 약물반응군은 81.3%였다. 리스페리돈에 반응하는 예측인자로는 발병연령, 이전의 입원 횟수, 유병기간이 관련 있었다. 리스페리돈은 1주후부터 PANSS양성, 음성, 및 일반정신병리 점수상에 유의한 호전을 보여 효과가 빨랐다. CGI의 경우도 기준점에 비해 1주후부터 유의한 감소를 나타내었다. ESRS의 경우, 파킨슨 평가점수는 기준점과 비교해 투여 1주, 2주, 4주후 유의하게 증가되었다가 8주후 기준점과 차이가 없었다. Dystonia 평가점수는 1주후만 유의한 증가를 보였으며, dyskinesia 평가점수는 유의한 차이가 없었다. 혈압, 맥박수의 생명징후 및 일반 혈액학 검사, 생화학적 검사, 심전도 검사에서 유의한 변화는 없었다. 결 론 : 이상의 다시설 개방 임상 연구를 통해 리스페리돈은 정신분열병 환자에서 양성증상뿐만 아니라 음성증상 및 전반적인 증상에도 효과적인 것으로 사료된다. 보다 명확한 평가를 위해서는 다른 항정신병약물과의 이중맹검 연구가 필요할 것으로 생각되며, 또한 장기적 치료에 대한 평가도 함께 이루어져야 하겠다. Objective : The purpose of this study was to investigate the efficacy and safety of risperidone in the treatment of Korean schizophrenic patients. Method : This multicenter open study included 377 schizophrenic patients drawn from 39 university hospitals. After a wash-out period of 1 week, the schizophrenic patients were treated with risperidone for 8 weeks and evaluated at 5 points ; at baseline, and 1, 2, 4 and 8 weeks of treatment. The dose was increased from 2mg/day(1mg twice daily) to 6mg/day(3mg twice daily) during the first week and adjusted to a maximum of 16mg/day over the next 7 weeks according to the patient's clinical response. Medication to control extrapyramidal symptoms was permitted. The psychiatric and neurological status of the patients was assessed by PANSS, CGI, and ESRS scales. Results : 343(91%) of 377 patients completed the 8-week trial period. Clinical improvement, as defined by a 20% or more reduction in total PANSS score at end point, was shown by 81.3% of patients. The predictors of response to risperidone were associated older age, shorter duration of illness, fewer previous hospitalization. Risperidone had rapid onset of action ; a significant decrease of the total PANSS and three PANSS factor(positive, negative, general), and CGI was already noticed at the end of first week. For the ESRS, parkinsonism rating scores were significantly increased until week 4 comparing with baseline. Dystonia rating scores were significantly increased until week 1, and dyskinesia rating scores were not significantly changed during the study. Laboratory parameters including vital sign, EKG, hematological, and biochemical values showed no significant changes during the trial. Conclusions : This study suggests that risperidone is generally safe and effective against both the positive and negative symptoms in our group of patients.

血淸 및 호르몬 添加가 소 卵胞卵의 體外成熟에 미치는 影響

尹鐘澤,鄭英彩,金昌根 中央大學校 遺傳工學硏究所 1991 遺傳工學硏究論集 Vol.4 No.1

These studies were carried out to investigate the effects of sera and hormones added to maturation medium on the in vitro maturation(IVM) in bovine follicular oocytes. The results obtained were as follows: 1. The IVM rate of oocytes was increased by addition of 10 to 15% FCS to m-KRB and DM and 20% FCS to Ham's F10, respectively. The addition of ECS to TCM199 was superior to that of FCS, but no difference in the IVM rate between ECS and FCS added to m-KRB. 2. The IVM rate in the media added with hormones was increased and the rate in m-KRB was higher when FSH was added to medium instead of HCG. The highest IVM rate was obtained in the TCM199 added with 10% ECS and LH. 3. The IVM rate of oocytes in the media added with sera was higher than that of hormones. 4. In conclusion, the present studies indicate that the addition of sera and hormones can improved the in vitro maturation of bovine follicular oocytes.

혈뇨의 감별진단을 위한 이형적혈구의 형태학적 연구

윤철종,박정오,문희주,윤기은 韓國電子顯微鏡學會 1999 Applied microscopy Vol.29 No.3

전체 성인의 약 4%에서 정도 차이는 있지만 혈뇨가 있다고 볼 때 그 원인을 구명하는 일이 중요하다. 그 원인이 사구체성 신염인지 기타 그 외의 비사구체성 원인에 따른 질환인지를 쉽게 감별하는 일이 중요하다. 본 연구자들은 혈뇨를 동반한 소아과 환자중에서 신 생검 조직검사까지 한 5명의 환자에서 혈뇨를 표본 제작하여 먼저 위상차현미경적 표본제작법을 통하여 적혈구를 감별하였고 방법을 달리하여 주사형 전자현미경으로 요침사를 관찰하여 형태학적 고찰을 통하여 이형적혈구를 보다 명확한 3차원구조를 관찰할 수 있었다. 침생검을 통해 얻은 조직학적 소견으로는 사구체의 기저막을 통과한 혈구들의 요로를 통하여 배출과정을 광학현미경 및 투과형 전자현미경으로 확인할 수 있었다. 본 연구에서 육안적 혈뇨의 색은 유출되는 적혈구의 양과 상관관계가 있으며 용혈된 정도가 심한 경우에 콜라색 혈뇨가 있음을 알 수 있었다. 이들 결과에서 본 연구자들은 사구체성 질환을 동반한 유극적혈구 및 환형 적혈구 등과 같은 변형된 적혈구를 주사형 전자현미경으로 명확한 관찰을 함으로 사구체성 질환으로 인한 혈뇨의 감별에 주사형 전자현미경이 가장 유용한 검사 방법이라고 생각된다. Examination of the morphology of red blood cells in the urine has been shown to be a promising adjunct in determining whether hematuria represents glomerular or nonglomerular bleeding. This is due to distortion of RBCs as they pass across the basement membrane of the glomerular capillaries. It is concluded that is method can greatly help the clinician in distinguishing between glomerular and non glomerular bleeding in patients with hematuria and channeling such patients toward the most appropriate investigations. We have experimented dysmorphic red blood cells that 5 patients of the hematuria are distorted with irregular outlines and often have small blebs extruding from the red cell membrane. Tried urinary sediments were seen with phase contrast microscope and confirmed scanning electron microscope. There are seen acanthocytes, anulocytes, ghost cells and sphero-echinocytes in dysmorphic erythrocytes. Clinical diagnosis was referred from the result of the biopsy-proven. Scanning electron microscopic findings of the hematuria are good diagnostic tool that disclose in distorted red blood cells from patients with glomerular disorders.

韓國農村의 近代化와 小作農에 관한 硏究 : 嶺南 地方을 中心으로

鄭喆洙,金種震,尹槿燮 慶北大學校 東洋文化硏究所 1978 東洋文化硏究 Vol.5 No.-

1. Duration of the Project: fron June 1977 to July 1978 2. Objectives of the Project: 1) An empirical survey on the real state and practices of the tenancy in rural community. 2) An analysis of the mobility of tenants and the relationships which exist between tenants and landlords. 3) Determining the orientation of the tenants' pattern of thought. 4) Examination of socio-economic factors related to the retardation of agricultural modernization under farm tenancy. 3. Methodology: Fourty rural villages, of these three types-villages near a city, in a plain and in a mountainous remote areas-have been selected for this research in Kyungsangbuk-do and Kyungsangnam-do areas. A tota1 of 3166 households were doing farming as their main occupations. Out of these 3166 farm households, there were 878 small tenant farm households (27.7%) which iuclude pure tenants, tenant farmers with their own farms, own farmers with tenant farms, ancestor worship farm tenants and others. Two thirds of these 878 tenant farmers (585 household heads) were selected for this research. However, a total of 514 tenants (responding rate:88%) out of 585 tenants were possible for interview. These 514 tenant farmers were subjects of analysis for this research. The findings are summarized in the following: (1) To see the ratio of tenant farm households to the total farm in suburban willages which are located near a city (48.5%); the next remote villages located in remote mountainous areas (23.1%) and plain villages located in a plain (20.6%). The reason for these differential ratio of tenant farmers in different types of villages may be explained in terms of rich urban residents' capability to buy farmlands near their living cities and then to rent those farms to the vi11age tenant farmers. (2) Around 90% of 514 respondents are males and they are mainly in their 40s and 50s(65%). 82% of them had less than elementary school education. There are 6.5 persons per household on the average. This number of average household members exceeds average number of Korean household members by 1.4 persons (1975). (3) 68% of all respondents farm less than one jongbo of arable land per house hold. Extremely poor farmers who farm less than 5 danbo are 22% of all respondents. Among these figures, pure tenant farmers are almost half of these extremely poor farmers(47%). As a whole, the size of tenant farmlands per household is: less than one jongbo(93%), less than five danbo(97%). The ratio of tenant farm area to the total average arable land per household is 57%. (4) Annual average income per household is: less than 700,000 won (55%), more than 1,000,000 won(24%). 87% of those pure tenant farmers, on the average, record an annual average income of less than 700,000 won. Compared with an annual average income of 1,430,000 won of Korean rural residents per household(1977), A majority of tenant farmers are earning less than half of the Korean farm household annual income in general. (5) 88% of all respondent farmers live in their own houses. However, only 65% of pure tenant farmers live in their own houses. Cultural conveniences include radio and TV (16% respectively), newspaper subscription (16%), and use of toothpasts(2.5%). (6) 65% of respondents have lived in their present villages since before August 15th(1945) Korean Liberation Day (i.e. for more than 30 years they have lived in the villages); These farmers born in their village comprise 31% while immigrants from other places are 69%. Out of these 69% of tenant farmers who moved in the present villages, a majority of them(90%) are from rural areas of their province of origin. (7) Social mobility of tenant farmers: 68% of respondents were farmers as thier previous occupations, and about 18% of respondents were front non-agricultural backgrounds (mannual laborers, agricultural laborers, salers, and tne non-employed). Around 23% of respondents who were landlords before the Land Reform showed downward social mobility by becoming tenant farmers after the Land Reform. About 21% of respondents showed reverse social mobility trend, i.e. upward social mobility from farm laborers or farm servants. Those tenant farmers whose fathers were own farmers or landlords were about 40% of all respondents while those who showed intergenerational upward mobility from employed laborer status to tenant farming covers only six per cent of all respondents. (8) Practice of tenancy has a long history in Korea, and the three different types of tenancy in general practice are: 1) fix ground rent (Jongdo-bob) which tenant farmer pays a certain amount of rent to the landlord regardless of good of bad harvest, 2) after-harvest rent (Tajak-bob) in which a certain amount of tenant rate is predetermined in advance, however, the actual amount of crops may vary depending upon the amount of crops well or illharvested, 3) before-harvest rent (Doji-bob) in which tenant rent is decided upon just before the harvest mainly by the estimates of the crops by tenant farmers and landlords. In this research we find that 49% of respondents are in after-harvest rent practice 42% in fix ground rent practice and 6% in before-harvest rent practice. Eighty-two percent of tenant farmers pay their rents by way of rice crops and only 13% of them pay the rent in cash. In the case of the after-harvest rent practice, 88% of the tenant farmers divide the harvest crops in half with the landlords. A total of 79 tenant farmers (15%) out of 514 tenant farmers pay some part of between-rice crops as their tenant rents in addition to the heavy main rents. In varying degrees, the tenant farmers pay land taxes(52%), and water tax or seed expenses and fertilizer cost (85%). Almost 47% of tenant farmers bear half of the lenses of carrying the rent crop to the landlord's house. 32% of tenants carry the rent crop to the landlord's house with tenant's expenses. (9) Tenant period tends not to be definite in terms of contracts (68%). About 89% of tenants rent the farms from the landlords by oral contracts. Around 87% of the tenants pay their rent after the harvest is completed. (10) Half of the tenant farmers have no kinship relations with their landlords. Around one third of the tenant farmers rent farms from close kinsmen including parent-child relations. Around 68% of landlords live in the villages, while 29% of landlords live in the cities. The landlords control the tenant frmers directly without brokers between them. (11) On the questions of their attitudes toward farming occupation, around 60% of tenant farmers expressed dissatisfaction of their farming occupation. About 40% of them satisfied themselves with their occupation, and equally about 40% of them cited farming or agriculature as their desired occupation. Asked what occupation they want their children to work on, they cited govern ment offices, teaching, sales. Agriculture or farming found the lowest scale of the desire. Judging from these phenomena, the tenant farmers do not like farming inside their heart. Therefore, those tenant farmers who really like working on the farm are not many. (12) Around over one third of tenant farmers think there are some differences in the harvest amount between their own lands and their related lands. The main reason for this pattern of thought seems to be in their attachment to their lands. Around 38% of those respondents answered in the positive when asked whether they could produce more harvest if their tenanted lands were their own lands. The majority of tenant farmers responded favorably to the Land Reform. In the above we reviewed the real state of tenancy in Korea. Although around 30 years have passed since the Land Reform in 1949, we have unfortunately found the actual existence of landlord-tenant relations which are forbidden by law. The existence of tenancy impedes the modernization of rural Korean community. Those tenant farmers who have only less than 24,506 acre of lands to cultivate are 70% of all respondents. These tenant farmers who pay 50% of their harvest as their rent to the landlords are around 90% of all the farmers interviewed. In addition, may of those tenants should bear the expenses of land taxes, water tax, seed purchase, fertilizer, other rents and the burden of carrying the crop rent to the landlords. Thus the majority of their annual average income are lower that half of that of Korean rural community in general. That is, their annual average income is only meager 700,000 won, forming lower income strata. However, these tenant families have 1.4 persons more members per family compared with the number of persons per family of the rural Korean community average in general. Thus, their subsistance faces further difficulty. These tenants, economically in difficult conditions, are also insulated from the cultural benefits of modernization. Their educational level shows that 80% of them had only less than 6 years of formal education. The fact that 16% of them posess radios and only 2.5% of them use toothpastes for brushing the teeth demonstrates their cultural deprivation. On the other hand, concerning the landlord tenant relationships, the oral contact dominate between the two parties, and no period of tenancy is decided in the beginning. Therefore we can safely see the practice of pre-modern patterns of practices. We think that the tenants still maintain the pre-modernization patterns of thought which can be seen in an answer by tenants to question on, what amount of rent would be desirable; around 58% of tenants said the proper amount of rent would be more than 40% of crops harvested. In short, for the rapid realization of Korean modernization and democratization, it is necessary to destroy the landlord-tenant relations according to the principle of "land to the tiller." In case of its difficulty to carry it out soon, at least we should see to it that the high rate of rent should be reduced to the level which may ease heavy burden of tenant farmers. These, measures should be taken steps soon so that the tenant farmers may enhance their level of life as well as enjoy the cultural benefits. These measures will help change some pattern of thought of tenant farmers.

컴퓨터를 이용한 유기용제 폭로 근로자의 신경행동학적 장애 검사

정종학,김창윤,사공준 大韓産業醫學會 1994 대한직업환경의학회지 Vol.6 No.2

유기용제폭로 근로자에 있어서 신경행동기능과 자각증상을 평가하기 위하여 1992년 10월부터 경상북도 구미시의 모 비디오테이프 제조공장에서 톨루엔, 시클로헥사논, 메틸 에틸 케톤에 주로 폭로되는 근로자들을 폭로군(고폭로군 45명, 저폭로군 44명)으로 하고 동일 공장 내 다른 부서의 생산직 근로자 43명을 대조군으로 하여 단면조사연구를 실시하였다. 신경행동검사는 개인용 컴퓨터와 신경행동검사용 컴퓨터 프로그램 Swedish performance evaluation system을 이용하여 단순반응시간(simple reaction time), 선택반응시간(choice reaction time), 숫자구분(digit classification), 손가락민첩성(finger tapping speed), 숫자더하기(additions), 부호숫자짝짓기(symbol digit)와 숫자외우기(digit span), 7가지의 신경행동검사를 시행하고 인구학적 변수와 작업과 관련된 변수를 조사하였다. 16문항의 설문지(Scandinavian questionnaire 16)와 신불안우울척도(New Anxiety - Depression Scale)를 이용하여 자각증상과 감정상태를 측정하였다. 조사대상의 평균연령은 29세, 평균근무년수는 5.4년으로 세 군간에 차이가 없었으며 83%가 남자였고, 85%가 고등학교 졸업자였다. 자각증상에 있어서 연령, 성, 교육수준, 음주여부를 보정한 후 유기용제 폭로수준에 따라 호소율의 차이를 보이는 항목은 없었고 총 증상점수 역시 폭로수준에 따라 유의한 차이가 없었으며 신불안우울척도에 있어서도 문항이나 총점수에서 유의한 차이가 없었다. 신경행동검사의 단순비교에서 선택반응시간, 숫자구분, 숫자더하기, 부호숫자짝짓기의 반응시간이 폭로수준이 증가할수록 유의하게 길어졌으며 단순반응시간, 손가락민첩성, 숫자외우기는 유의한 차이를 보이지 않았다. 측정치 중 반응시간의 표준편차나 잘못 반응한 횟수는 세군간에 유의한 차이를 보이거나 일정한 경향을 나타내지 않았다. 신경행동검사 항목들에 대한 요인분석에서는 숫자더하기, 부호숫자짝짓기, 숫자외우기가 "기억력관련요인", 양 손의 손가락 민첩성이 "협조운동관련요인", 단순반응시간, 선택반응시간, 숫자구분이 "반응속도관련요인"으로 분류되었으며 세 요인에 의한 설명력은 62.6%였다. 연령, 성, 교육수준 및 컴퓨터에 대한 친숙도 등을 보정한 후에도 선택반응시간, 숫자구분, 숫자더하기, 부호숫자짝짓기의 반응시간이 폭로수준에 따라 유의한 차이를 보였으며 열수의 손가락민첩성과 "기억관련요인", "반응속도관련요인"의 요인점수가 유의한 차이가 나타내었다. 잘못 반응한 횟수는 남자보다 여자에서 많았으며 교육수준이 낮을수록 복잡한 검사에서 잘못 반응한 횟수가 많았다. 컴퓨터에 대한 친숙도가 클수록 잘못 반응한 횟수가 적고 비교적 복잡한 검사항목에서 반응시간이 짧았다. 흡연군이 비흡연군에 비해 손가락 민첩성에서 두드림 횟수가 유의하게 적었다. 자각증상의 호소율은 신불안우울척도와는 높은 상관관계를 보였으나 신경행동검사 측정치와는 낮은 상관관계를 보였고 증상의 호소율이 높을수록 반응시간은 길어지고 두드림 횟수가 적어지며 숫자외우기의 숫자의 길이가 짧아지는 경향을 나타내었다. 7 가지 검사항목 중 부호숫자짝짓기의 반응시간이 폭로수준, 연령, 성, 컴퓨터에 대한 친숙도의 차이에 따라 가장 민감하게 변화하였다. A cross-sectional study was performed to evaluate the effects of chronic exposure to solvent on neurobehavioral performance of solvent exposed 89 workers engaged in a video tape manufacturing factory. A control group of 43 workers was selected from different sections of the factory. The mean age ad the mean duration of employment were 29 years and 5.4 years. Each worker completed a medical and occupational questionnaire. Adverse effects on the central nervous system were assessed by self reported Scandinavian questionnaire 16, New Anxiety - Depression Scale and seven tests of Swedish Performance Evaluation System. These included simple reaction time, choice reaction time, digit classification, finger tapping speed, additions, symbol digit and digit span. Several potential confounding factors were included as independent variables in all analysis of neurobehavioral tests and symptoms. These included age, sex, educational level, familiarity with computer, alcohol drinking and amount of smoking. No significant associations were found between solvent exposure and total symptom score and mood score. Although the mean duration of employment was 5.4 years, comparison of mean performance scores for confounding factors showed a significantly poorer performance on choice reaction time(P<0.05), digit classification(P<0.01), additions(P<0.01) and symbol digit(P<0.01) in exposed group. Factor matrix of neurobehavioal tests produced by varimax rotation showed three distinct factors, which correspond to assessments of "memory", "coordination" and "response speed". These factors accounted for 62.6% of the total variance of the sample. Familiarity with computer was found to be associated with performance of symbol digit and amount of smoking appeared to slow finger tapping speed. Male and college graduate workers have less errors than others. These were no significant correlation between neurotoxic symptom score and performance of neurobehavioral tests.

고양이 감각 및 운동겉질에서 calbindin D-28k와 parvalbumin에 대한 면역세포화학적연구

김종중,장인엽,정윤영,윤상필,문정석 대한해부학회 1998 Anatomy & Cell Biology Vol.31 No.2

한국의 광물명칭에 관한 연구

정원우,상기남,이윤종,김중욱,곽기근 慶北大學校 科學敎育硏究所 2003 科學敎育硏究誌 Vol.27 No.-

The purpose of this study is to investigate historical records on Korean mineral terms. One of the earliest records on Korean mineral terms is the Donguebogam(oriental medical handbook) written by Heo Jun in 1613. Most of geological investigation of Korea was accomplished by Japanese geologists in the period of the 1900~1945. Korean mineralogical records have been described by Gottsch(1886), Kwasaki(1935), Kinosaki(1937, 1940), Tsuda(1941, 1952) and Galleger(1963). Korean mineral species have been described about 300 species by KIER(Korea Institute Energy and Resources) in 1977. In the results of this study, Comparisons and classifications between current korean mineral terms and korean traditional and chinese medical mineral terms are suggested.

노화흰쥐의 해마에서 parvalbumin과 calbindin-D28K의 면역반응세포에 관한 연구

정윤영,김종중 朝鮮大學校 附設 醫學硏究所 2009 The Medical Journal of Chosun University Vol.33 No.S

Aging of the hippocampus has been characterized by generative changes of fiber connetions, cell loss, and involved memory and learning impariment, cognition deficit. Aging leads to alternation in activity of parval-bumin (PV) and calbindin-D28k(CB) expressing in the brain of the rats. Two of calcium binding proteins (CBPs), PV and CB are, widely expressed in the adult central nuervous system. The aim of the present study is, therefore, to investigative possible changes in the number and morphology of PV and CB-immunoreactive neurons with aging in each area of the hippocampus of aged rats. The number and density of PV-immunoreactive neurons in the dendate gyrus and CA1 subregion was decreased in the hippocampus in aged group when compared with the young group. But, the number of PV-immunoreactive neurons in the CA3 subregion of the aged group was similar to that in the young group. And, excepting the CA1 subregion, the number of CB-immunoreactive neurons was not practically different in the young group when compared with the aged group. These results showed the alterative expression of the two CBPs during aging process of hippocampus. The age-related reduction of CBPs in hippocampus subregion is likely to deprive neurons from the capacity to better intracellular calcium and to leave them vulnerable to pathological processes.