발효조를 이용한 Monascus anka의 적색소와 황색소의 생산

강성국,임종환,정순택,김선재 한국미생물생명공학회 ( 구 한국산업미생물학회 ) 1996 한국미생물·생명공학회지 Vol.24 No.6

Monascus anka를 이용하여 천연식용색소를 대량생산하기 위한 방법을 개발하기 위하여 발효조를 이용하여 적색소와 황색소의 색소생산의 최적 배양 조건과 생산된 색소의 색깔 특성을 조사하였다. Monascus anka 적색소와 황색소를 생산하였는데, 이들 색소는 균체내색소와 균체외 색소로 구분되었다. 균체의 적색소(ERP)는 494nm에서, 균체외 황색소(EYP)는 380nm에서, 균체내 적색소(IRP)는 506nm에서 그리고 균체내 황색소(IYP)는 388nm에서 최대 흡광도를 나타냈다. 적색소와 황색소 생산, 색조의 특성 및 균체증식력을 배양 온도, pH, 쌀가루 농도, peptone 농도, magnesium sulfate 농도, 통기량 및 교반속도에 대하여 조사한 결과 적색소 생산력은 30℃, 초기 pH 6.0, 쌀가루 농도 3~5%, peptone 농도 0.05%, magnesium sulfate 농도 0.25%, 통기량 0.1vvm 교반속도 300rpm의 조건에서 최대치를 보였으며, 이때 ERP, EYP, IRP 및 IYP는 각각 A_494nm 0.84, A_380 nm 0.71, A_506 nm 1.18, A_388 nm 1.10, L값은 각각 29.40, 6.44, 34,98, 8.94, a값은 각각 5.76, -1.08, 11.83, -1.55, b값은 각각 18-19, 3.41 27.26, 6.16 그리고 균체량은 7.4 g/l을 보였다. 또한 황색소 생산력은 온도의 경우 적색소 생산을 위한 최적온도 보다 높은 35℃ 부근에서 우수하였으며 초기 pH와 쌀가루 농도는 적색소 생산을 위한 최적조건과 같았고, 질소원과 무기염의 농도가 높을수록 그리고 통기량이 많을수록 황색소 생산력이 우수하였다. 적색소와 황색소 생산을 위한 발효조의 교반속도는 100~300 rpm이 적합하였다. Production of Red and Yellow Pigments from Monascus anda in a Jar Fermenter, Seong-Gook Kang, Jong-Whan Rhim, Soon-Teck Jung^* and Sun-Jae Kim. Department of Food Engineering. Mokpo National University Chonnam, 534-729, Korea - In order to develop the method for mass production of natural food colorant from Monascus anka, optimum cultivation conditions for producing red and yellow pigments by cultivating the mold in a jar fermenter and their color characteristics were investigated. The mold produced red and yellow pigments both intracellularly and extracellularly. These pigments showed unique light absorption characteristics with maximum absorption of 494, 380, 506, and 388 nm for extracellular red pigment (ERP), extracellular yellow pigment (EYP), intracellular red pigment (IRP), and intracellular yellow pigment (IYP), respectively. Optimum conditions for producing red pigments were found to be temperature 30℃, initial pH 6.0, rice powder 3~5%, peptone 0.05%, magnesium sulfate 0.25%, aeration rate 0.1vvm. Optimum temperature for producing yellow pigments was around 35℃ which is higher than that of producing red pigments. The initial pH rice powder concentration for producing yellow pigments were the same as those of producing red pigments. The higher concentration of nitrogen source and inorganic salt, aeration rate, the more the yellow pigments were produced. Them optimum agitation speed was 100~300 rpm for pigment production.

SEM 측정법에 의한 초정밀 표면가공 특성연구

강순준,오상록,이갑조,김종관 한국공작기계학회 2004 한국공작기계학회 춘계학술대회논문집 Vol.2004 No.-

The purpose of this paper is to look at the characteristics of surface finishing which is one of the form accuracies and to obtain the fundamental technical data from the process of machining with diamond tool through experiment and theoretical analysis. The experiments were conducted with domestic made ultra-precision machine and MCD·PCD tool, with aluminum alloyed material and brass being used for the work pieces. The goal of the size accuracy was set to 100nm. The most suitable tool nose radius and machining conditions were selected, and the variations of the surface roughness were observed using SEM method while machining the distance of up to 500km. These data were evaluated and they examined the variation of the machined surfaces while cutting up to 500km of machining distance. At the same time, the state for the wear of diamond tool nose was analyzed and carefully examined through the newest measuring device. Additionally, the characteristics of ultra-precision machining technology were studied through visual analysis.

초정밀 절삭가공에서 표면거칠기 특성 평가

강순준,김종관 한국공작기계학회 2004 한국생산제조학회지 Vol.13 No.1

In this study. experiments were conducted with an ultra-precision machine. developed in domestic. to find the characteristics and the most suitable cutting conditions of ultra-precision machining. To maximize the performance of the machine, the machine was installed in a room that is protected from vibration and is maintained constant temperature and constant humidity. Selected work pieces are an aluminum-alloyed material, which has excellent corrosion resistance and has loll deformation. The used tool is synthetic poly crystal diamond, which has excellent abrasion resistance and has low affinity, Four types of tool nose radius were used such as 0. 0.1, 0.2 and 0.4mm. machining is performed with cutting speed of 500, 800 and 1000m/min. feed rate of 0.005, 0.008, 0.01mm/rev. and cutting depth of 0.0005, 0.0025 and 0.005mm respectively which can generally be used in the field as a cutting condition. As a method of evaluation, surface roughness was measured for each cutting condition. and reciprocal characteristics are computed for each tool nose radius. cutting speed, feed rate and cutting depth. As a result. the most suitable cutting condition and characteristics of ultra-precision machining were identified which can usefully be applied in the industrial field.

外筒과 윗板이 回轉하는 二重圓筒內에서의 定常層流 流動解析

姜昌壽,權純範,李鍾鵬 경북대학교 공과대학 1981 工大硏究誌 Vol.10 No.-

In this paper, steady laminar flow in a tank of double cylinder with rotating outer cylinder and upper disc was analyzed numerically. For simplicity, the stream function and vorticity have been introduced into the Navier-Stokes and the continuity equations. The transformed governing equations were converted into the fimite difference equations which were solved numerically by ADI (Alternating direction implicit) and SUR (Successive under relaxation) method.

OFDMA 기반 이동통신시스템을 위한 핸드오버 알고리즘

강창순,염동화,석종원 국립7개대학공동논문집간행위원회 2005 공업기술연구 Vol.5 No.-

The wireless broadband (WiBro) system supports high quality internet data services with medium user's mobility. Towards this end, the system uses orthogonal frequency division multiple access (OFDMA) technology and the same frequency in every cell. To provide high-speed seamless services, handover functionality is needed. In this paper a handover algorithm for the WiBro system is proposed. Two kinds of OFDM channels are considered in the algorithm : diversity channel and safety channel. The handover algorithm is composed of intra-cell handover and inter cell handover using safety channels. In particular, the safety channel is used for the mobile users when the received signal quality is degraded. Performance analysis results show that the quality of service can be significantly improved by reducing the interference effect among OFDM channels.

HPLC-ECD를 이용한 흰쥐의 뇌 중 감마아미노부티르산 및 글루탐산의 정량

강종성,이순철 충남대학교 약학대학 의약품개발연구소 1999 藥學論文集 Vol.15 No.-

A sensitive and efficient assay method was applied to determine the level of glutamic acid (GA) and γ-aminobutyric acid (GABA) in frontal cortex and hippocampus of rat administrated with ethanol and drugs. The compounds were derivatized with ο-phthalaldehyde (OPA) and 2-mercaptoethanol for precolumn analysis. The condition for the simultaneous determination of GA, GABA and beta-aminobutyric acid (BABA) by high performance liquid chromatography with electrochemical detection was reverse phase C_18 column as stationary phase, 0.1M phosphate buffer containing 0.1mM Na_4EDTA: methanol=55:45 (v+v) pH 3.8 as mobile phase and 0.7V electrode voltage. The stability of reaction product of GA, GABA and BABA with OPA could be increased by adding the same volume of polyethylene glycol 400 to reaction mixture. The GABA level in frontal cortex of rat was significantly decreased by the administration of picrotoxin and diazepam, but it was significantly increased by the administration of red ginseng total saponin, N-methyl-D-glucamine and (-)-deprenyl.

인지질말과 콜레스테롤을 포함한 인지질막의 수용액에서의 산화반응 속도에 관한 연구

康順姬,朴鐘倫,金健,盧貞銀 梨花女子大學校 韓國生活科學硏究院 1988 韓國生活科學硏究院 論叢 Vol.42 No.-

생체막의 중요 성분중의 하나인 지질의 산화반응에 대한 연구로 모델 생체막인 인지질 이중층막을 제조한 후 pH 7.5 완충용액에서 KMnO_4로 산화시킬 때의 속도는 근사 일차 속도식으로 나타났다. PC 이중층막의 산화반응 속도 상수를 25℃에서 40℃ 온도 구간에서 얻어낸 결과 급격한 속도 상승 증가 현상이 PC 이중층막의 상전이 온도 구간에서 관측되었다. 그리고 PC에 콜레스테롤 10mol% 포함한 혼합 이중층막의 산화 속도 상수는 PC 이중층막의 산화속도 상수보다 모든 온도구간에서 크게 나타났다. The kinetics of oxidation of phosphatidyl choline(PC)and PC-cholesterol-reconstituted vesicle on water has been studied at 20°, 25°, 30°, 35°, and 40℃. The oxidation was initiated by KMnO_4 in pH 7.5 Tris-buffer aqueous solution and the product was measured by UV absorption at 230nm. The kinetics of oxidation of PC-vesicle and PC-cholesterol-reconstituted vesicle by KMnO_4 was pseudo-first order reaction. The oxidationof PC-cholesterol reconstituted vesicle was faster than that of PC vesicle. The highest difference of rate constants of PC vesicle was observed in the range of the phase transition temperature (35∼40℃) of PC-vesicle.

초기치 문제를 극복한 그림자 정보의 형상복구

강창순,배종갑 동의공업대학 1999 論文集 Vol.25 No.1

In this paper, we propose the new reconstruction algorithm of shape from shading without regularization. The proposed algorithm can overcome the initial value problem by using the singular point for the estimation of the surface normal. In our approach, it is assumed that the illuminant source direction is known and the object surface is uniform Lambertian. The normal vector of the given image pixel is calculated from the illuminant direction vector, the brightness of the image, and the singular point that has the local maximum of brightness. Computing speed in our method is faster than in other ones, since normal vectors are computed directly. In addition, it gives good results for the convex surfaces in contrast to other methods. Simulation results from synthetic image are provided to demonstrate the performance of the proposed algorithm and compare it with the performance of miminimum downhill principle.

음향궤환제거에 의한 보청기 성능향상

강창순,배종갑 동의공업대학 2001 論文集 Vol.27 No.1

This paper presents a new method of acoustic feedback cancellation in hearing aids which is based on the orthogonality principle. This method is less affected by ambient signals in steady state, therefore the adaptive filter estimates the feedback path well. The improved performance of this method is confirmed by computer simulation.

철봉 드가체프 동작의 운동학적 분석

강순용,박종진,김창욱,최성진 한국운동역학회 2001 한국운동역학회지 Vol.11 No.1

The purpose of this study is to investigate the kinematical variables of the Tkatchev motion on the horizontal bar and the effects of kinematical variables of 1 phase on 2, 3, 4 phases and the effects of kinematical characteristics of every phase on motion the air and re-grasping. The subjects Tkatchev motion were filmed with S-VHS camera at the speed of 60 fields per second and each field is digitalized to 5 fields with from the sum of handstand motion to landing. The Kwon 3D 2.1 version program is employed to obtain 3-dimensional data. Based on the results of this study, the following conclusions are drawn. 1. In the Down swing phase, the maintenance of great vertical downward velocity is an important variable to achieve large angular velocity of body center and it is also proved that the fast downward motion gives advantage in spreading body joints to get great motion power in the next Whip swing motion. 2. In the Whip swing phase, fast upward velocity and fast angular velocity of hip joint will positively affect upswing movement in t he phase. To shorten the distance between horizontal bar and body center helps effective upward motion as well. These also work beneficially in the next phase. 3. In the Releases phase, fast vertical upward velocity until pull back after curving shoulder joint and hip joint at the whip swing and large change of position of body center are important variables to perform high and long flight duration. 4. In the Re-grasp phase, the maximum spread of shoulder joint and hip and the spread of body center to the opposite direction of it helps body center to stay in higher place and to perform large circular flight motion with extended length of time. This ultimately makes effective re-grasping motion happen and connects next motion smoothly.