Aptamer‑linked immobilized sorbent assay for detecting GMO marker, phosphinothricin acetyltransferase (PAT)

Woo‑Ri Shin,Mun‑Jong Lee,SEKHONSIMRANJEET SINGH,Ji Hun Kim,Sun Chang Kim,Byung‑Kwan Cho,Ji‑Young Ahn,Yang‑Hoon Kim 대한독성 유전단백체 학회 2020 Molecular & cellular toxicology Vol.16 No.3

Background Development of genetically modified crops has rapidly increased in last few years. The most widely grown GM crops express genes that confer herbicide tolerance and insect resistance. Detection system of GM crops is important for safety evaluation before its consumption. Objective The purpose of this research is to detect GM crops, especially PAT, in food-samples. Results The bar gene (PAT protein, herbicide resistant) was cloned in pGEX-4T-1 and expressed by E. coli. The high-affinity PAT-specific single-stranded DNA (ssDNA) aptamers were obtained from a random DNA library. MOE docking study was performed to identify the potential binding region of the selected aptamers on PAT. Aptamer-linked immobilized sorbent assay (ALISA) method was used to detect PAT. Conclusion We screened aptamer against PAT for developing an efficient detection method. The selected PAT specific aptamers, HRPA-05 and HRPA-07, showed the distinct target binding behaviors, and detected PAT protein by aptamer-linked immobilized sorbent assay method with high efficiency and selectivity.

아미노글리코사이드 사용제한에 따른 병원성 균혈증 유발 그람음성간균의 내성변화 및 임상적 의의

오종택,김신우,손종원,도병훈,한승우,신병철,박지현,이종명,김능수 대한감염학회 2003 감염과 화학요법 Vol.35 No.4

목적 : 항균제 오남용으로 인한 내성균의 출현, 약물부작용의 발생 및 약제 비용의 증가는 환자 개인뿐만 아니라 사회적으로 문제가 되고 있다. 본 연구는 항생제의 오남용을 막기 위한 제도의 일환으로 시행한 아미노글리코사이드 사용 제한정책의 비용 효과적인 측면을 조사하고 그람음성간균에 의해 병원성 균혈증이 발생한 환자들을 대상으로 이 정책이 항생제 내성률과 환자 사망률에 미치는 영향을 연구하였다. 방법 : 930병상 규모의 3차 병원인 일개 대학병원에서 아미노글리코사이드 사용제한정책이 시행된 직후인 2002년 3월에서 9월 사이에 그람음성간균(Acinetobacter baumannii, Pseudomonas aeruginosa, Klebsiella pneumoniae, Escherichia coli, Enterobacter cloacae, Enterobacter aerogenes, Serratia marcescens, Stenotrophomonas maltophilia, Burkholderia cepacia)에 의해 발생한 병원성 균혈증 환자 60명(실험군)과, 이 정책이 시행되기 전에 입원한 환자들 중 균주별로 같은 수만큼 선별한 병원성 균혈증 환자 60명(대조군)의 의무기록을 후향적으로 조사하였다. 결과 : 아미노글리코사이드 사용제한정책이 시행된 시기인 2002년 3월에서 9월 사이에 처방된 아미노글리코사이드 사용량 및 비용을 이 정책이 시행되기 전인 2001년 3월에서 9월 사이와 비교한 결과, 아미노글리코사이드의 사용량(antimicrobial utilization density)은 사용제한 후 225.2에서 130.3으로 42% 감소하였고 약제 비용은 44% 감소하였다. 아미노글리코사이드 사용제한 후 병원성 균혈증을 유발한 그람 음성간균의 이 항생제에 대한 내성률은 유의한 변화를 보이지 않았으며 균혈증과 직접 연관된 사망률도 환자의 나이, 성별, 기저질환 및 원발병소 등을 보정할 경우 유의한 차이를 보이지 않았다(40.4% vs. 24.1%, P=0.11). 결론 : 아미노글리코사이드를 5일을 초과한 사용 시 감염전문가의 사전 승인을 필요로 하는 사용제한정책은 아미노글리코사이드의 사용량과 비용을 유의하게 감소시켰다. 또한 이 정책 시행 후 그람음성간균에 의한 균혈증 환자에서 균혈증으로 인한 사망률과 아미노글리코사이드에 대한 내성률은 유의한 차이가 없었다. Objective : To evaluate the effects of an aminoglycoside restriction policy on expenditures for aminoglycosides, antimicrobial resistance rates and clinical outcome of nosocomial bacteremia caused by Gram-negative bacilli (GNB). Methods : Starting in February, 2002, a prior consultation with an infectious disease specialist for using aminoglycoside antibiotics over 5 days was required in a 930-bed university hospital. In retrospective analysis of medical records 7 months after initiation of the aminoglycoside restriction policy, sixty cases of clinically relevant nosocomial bacteremia caused by GNB were found. These bacteremic patients were compared with sixty, species-matched, control patients with nosocomial Gramnegative bacteremia before the policy for total expenditures for aminoglycosides, susceptibility to antibiotics and clinical outcomes of bacteremia. Results : During the same period of 7 months before and after the restriction policy, total expenditures for aminoglycosides decreased by 44% in cost (from 465,030,841 Won to 259,618,337 Won) and the antimicrobial utilization density of aminoglycosides decreased by 42% (from 225,2 to 130.3). On the other hand, the patterns of antibiotic susceptibility and bacteremia-related in-hospital mortality rates after the policy did not show a significant change, compared with those before the policy. Conclusion : Antibiotic restrictions are among the most popular methods to diminish the practice of antibiotic overuse in hospitals. In this study, requirement for prior approval of aminoglycoside use over 5 days led to a significant decrease in the amount and cost of total aminoglycosides without a significant change in susceptibility patterns and bacteremia-related mortality rates.

뇌실외 배액술 시행과 관련된 뇌실염 : 원인균주와 경험적 치료약제의 적절성

도병훈,김신우,오종택,손종원,하상우,이응갑,장현하,이종명,김능수 대한감염학회 2005 감염과 화학요법 Vol.37 No.2

목적 : 본 연구는 뇌실외 배액술을 시행한 신경외과 환자들에서 뇌실염 발생 빈도, 원인균주와 감수성 추이 및 경험적 치료로 사용되는 항생제인 ceftazidime과 vancomycin의 적절성 여부에 대하여 조사하였다. 재료 및 방법 : 2000년 12월부터 2003년 10월까지 EVD를 시행한 경북대학교병원 신경외과 입원환자 340명 중 뇌실염이 발생한 39명을 대상으로 후향적으로 조사하였다. 결과 : 340명 중 39예 (11.5%)에서 뇌실염이 발생하였고 이들 가운데 4명이 뇌실염과 연관하여 사망하였다(10.3%). EVD 시행일로부터 뇌실염 발생까지는 평균 8.4일이었다. 원인균은 33예(87.1%)에서 확인되었으며 Aci-netobacter (19예, 45%), methicillin-resistant coagulase negative Staphylococcus (9예, 22%), methicillin-resistant Staphylococcus aureus (9예, 22%) 등의 순이었다. 가장 많이 동정된 Acinetobacter 19예 중에서 8예(42.1%)가 ceftriaxone에 내성을 보였으며 현재 경험적으로 널리 사용되는 ceftazidime에 대해서는 11예중 3예(27%)가 내성을 보였다. 결론 : 최근 EVD 시행과 관련된 뇌실염 뇌실염 최근 그람음성 non-fermenter 특히 Acinetobacter에 의한 경우가 증가하고 있으며, 이들 그람음성 non-fermenter는 현재까지 경험적으로 사용되는 ceftazidime에 점차 내성을 보이고 있다. 경험적 치료제에 대한 성공률을 높이기 위해서는 각 병원별 내실염의 원인균주의 감수성에 대한 검사가 필요하겠다. Purpose : To investigate the etiologic microorganisms of external ventricular drain (EVD)-related ventriculitis and the appropriateness of using ceftazidime and vancomycin as an empiric therapy in neurosurgical patients with EVD-related ventriculitis. Materials and Methods : Retrospective analysis of 39 patients with EVD-related ventriculitis among 340 neurosurgical patients to whom EVD had been placed during December 2000 and October 2003 at Kyungpook National University Hospital. Results : Thirty-nine EVD-related infections (39/340, 11.5%) occurred and the attributable mortality rate was 10.3% (4/39). The average duration from the ventricular catheter placement to the development of ventriculitis was 8.4 days. All patients with EVD infection had fever and 89.7% (35/39) of the patients showed nuchal rigidity. The positive culture rate in CSF was 87.1% (34/39) and the frequency of individual organism is as follows : Acinetobacter 45% (19 cases), methicillin-resistant coagulase negative Staphylococcus 22% (9 cases), methicillin-resistant Staphylococcus aureus 22% (9 cases), Enterococcus 5% (2 cases), Streptococcus pneumoniae 3% (1 case), non-fermenting gram-negative bacilli 3% (1 case). The polymicrobial infection rate was 15.4% (6/39). Among 19 cases of Acinetobacter infection, 42.1% (8/19) of the strains showed resistance to ceftriaxone and 15.7% (3/11) to ceftazidime. However, all cases were sensitive to meropenem. Conclusion : These findings show that the major etiologic organisms causing EVD-related ventriculitis have recently changed to Gram-negative non-fermenters, especially Acinetobacter. Because Gram-negative non-fermenting rods resistant to ceftazidime are increasing, an immediate change from ceftazidime plus vancomycin, the widely accepted empiric antibiotic therapy, to meropenem plus vancomycin should be considered when clinical symptoms and signs show no improvement or even deterioration.

한의진단명과 진단요건의 표준화 연구 II (표준화 실례) : 2차년도 연구결과 중간 보고

양기상,최선미,최승훈,안규석,박경모,박종현,김성우,신승호,정우열,전병훈,고현,김정범,신상우,김성훈,김동희,권영규,엄현섭,장혜옥 한국한의학연구원 1996 한국한의학연구원논문집 Vol.2 No.1

The diagnostic requirements were suggested and explained regarding the systems of differentiation of symptoms and signs in the second year study of standardization and unification of the terms and conditions used for diagnosis in oriental medicine. The systems were as follows; - differential diagnosis according to condition of body fluid, differentiation of syndromes according to the state of qi and blood, differential diagnosis according to relative excessiveness or deficiency of yin and yang(氣血陰陽津液辨證) - differentiation of diseases according to pathological changes of the viscera and their interrelation - analyzing and differentiating of febrile diseases in accordance with the theory of the six channels(傷寒辨證) The individual diagnosis pattern was arranged by the diagnostic requirements in the following order : another name(異名), notion of diagnosis pattern, index of differentiation of symptoms and signs(辨證指標), the main point of diagnosis, analysis of diagnosis pattern(證候分析), discrimination of diagnosis pattern(證候鑑別), a way of curing a diseases(治法), prescription(處方), herb in common use(常用藥物), diseases appearing the diagnosis pattern(常見疾病), documents(文獻調査). This study was carried out on the basis of the Chinese documents and references.

한국 정신장애의 역학 조사 연구[I] : 각 정신장애의 유병률

조맹제,함봉진,김장규,박강규,정은기,서동우,김선욱,조성진,이준영,홍진표,최용성,박종익,이동우,이기철,배재남,신정호,정인원,박종한,배안,이충경 大韓神經精神醫學會 2004 신경정신의학 Vol.43 No.4

Objectives : This study aims to estimate the prevalence of the DSM-IV psychiatric disorders in Korean population using the Korean version of Composite International Diagnostic Interview (K-CIDI). Methods : Subjects were selected by taking multi-stage, cluster samples of 7,867 adult household residents, 18 to 64 years of age, in ten catchment areas. Total 78 trained interviewers administered the K-CIDI to the selected respondents, from June 1 to November30,2001. Results : Total 6,275 respondents completed the interview. Some 33.5% of respondents reported at least one lifetime disorder, 20.6% reported at least one-year disorder, and 16.7% reported at least one-month disorder. The most common lifetime disorders were alcohol abuse/dependence (17.24%), nicotine dependence/withdrawal (11.19%), specific phobia (5.16%), and major de-pressive disorder (4.25%). The lifetime prevalence of substance abuse/dependence (0.25%) and schizophrenia (0.16%) was very low. Nicotine and alcohol use disorder showed very high male/female ratio. Mood disorder and anxiety disorder were more prevalent among female than male. Conclusion : The prevalence of psychiatric disorders was high. In comparison with other studies, remarkable differences in distributions of psychiatric disorders across the areas and times were observed.

Sprague-Dawley 백서의 소뇌 과립신경세포에서 Sevoflurane 에 의한 Baseline Potassium Channel 의 활성화

신우종,Winegar,Bruce D 대한마취과학회 2002 Korean Journal of Anesthesiology Vol.42 No.1

Background : Volatile general anesthetics have been widely used to produce reversible unconsciousness and analgesia in clinical practice over the last one hundred years, but the basic mechanism of anesthetic action is not yet completely understood. In addition to the well known mechanism of GABA_A and glycine channels, accumulating evidence indicates that neuronal baseline K^+ channels are also activated by volatile anesthetics. The goal of this study was to test the hypothesis that sevoflurane, one of the newly developed volatile anesthetics, activates baseline potassium channels in the cerebellar granule neurons of rats. Methods : whole cell measurement techniques were performed from cultured cerebellar granule neurons of seven day old male Sprague-Dawley rats using patch clamp techniques to see the effects of two MACs of sevoflurane on baseline K^+ channels. Holding potentials were set to -20 ㎷ and collect pulses from -90 to 90 in 10 ㎷ increments of 300 ㎳ duration. The electrode filling solution contained (in mM) 150 KOH, 105 aspartic acid, 3 Nacl, 10 HEPES, 86 glucose, 1 EGTA, 5 MgCl_2 (pH 7.4) and standard saline were used as bath solution. The bath contained 150 NaCl, 3 KCl, 10 HEPES, 12 glucose, 1 EGTA, 5 MgCl_2 (pH 7.4). Results : Analysis of multiple patch clamp experiments showed the presence of outward-rectifying K^+ selective ion channels with a conductance of 1.064 ± 0.32 nS (n = 10) at depolarized potentials. Cerebellar granule neurons exhibit rapid rising, noninactivating, outward-rectifying currents. These channels are insensitive to conventional K^+ channel blockers. Clinically relevant concentrations of sevoflurane (518 ㎛) increased the baseline K^+ channel outward currents from the control value by 225% in a standard saline perfusate (n = 10, P < 0.05, paired t-test). Channel activity enhanced during the duration of the exposure period to sevoflurane returned to the baseline activity level quickly upon wash. Conclusions : These outward-rectifying whole cell Ⅰ-Ⅴ curves are consistent with the properties of tandem pore K^+ channels. Activation of baseline K^+ channels in central neurons by two MACs of sevoflurane causes membrane hyperpolarization and increases neuronal input conductances providing an additional inhibitory mechanism that could contribute to the overall central depressant effects of this compound. (Korean J Anesthesiol 2002; 42: 107~114)

모형폐에서 폐유순도 변화가 기계적 조절환기에 미치는 영향

신우종,승익상 한양대학교 의과대학 1995 한양의대 학술지 Vol.15 No.1

Patients with acute respiratory failure requiring mechanical ventilation often undergo general anesthesia and surgery. The clinical feature of acute lung injury includes hypoxemia, hypercarbia, reduction in lung compliance and increase in work of breathing. In mechanical ventilation for these patients considerable volume from the anesthesia ventilator is not delivered to the lung, especially with lower complicance. In this study the effect of increasing respiratory frequency and tidal volume on the mechanical controlled ventilation of Draeger Remus A ventilator(Ventilog 2) was compared to identify minute volume and plateau inspiratory pressure by measuring at distal near the lung simulator and proximal end near the ventilator of breathing circuit in the 3 different states of lung compliance. Minute volume and plateau inspiratory pressure were simultaneously measured at the proximal end of breathing circuit near the ventilator and it's distal one near lung simulator under three study conditions using a simulated lung : 1) 50 ml/cmH2O of lung compliance and 5 cmH2O/1/sec of airflow resistance 2) 20 ml/cmH2O of lung compliance and 5 cmH2O of airflow resistance 3) 10 ml/cmH2O of lung compliance and 5 cmH₂O of airflow resistance. The results were as follows; 1. During controlled mechanical ventilation using anesthesia ventilator with any respiratory frequency and tidal volume, the change of intrapulmonary and total respiratory minute volume were directly related to the states of pulmonary compliance(p<0.05). 2. During controlled mechanical ventilation using anesthesia ventilator with any respiratory frequency and tidal volume in constant lung compliance, intrapulmonary minute volume was markedly less than total respiratory minute volume(p<0.05). 3. Generally there were differences between intrapulmonay and total respiratory minute volume and preset minute volume, and the difference was inversibly related to respiratory frequency and tidal volume, but proportional to lung compliance. 4. Intrapulmonary plateau inspiratory pressure in 20 and 10 ml/cmH2O of lung compliance was increased to approximately 1.5 to 2.0 and 2.5 to 3.0 times those in 50ml/cmH₂O of lung compliance respectively and about 0.5 to 1.5 times in total respiratory plateau inspiratory pressure. The above results suggest that there are statistically significant differences in minute volume and plateau inspiratory pressure between intrapulmonary and total respiratory system and that delivered minute volume to the lung actually did not reach to the level of preset minute volume especially in the lung with severe lowered lung compliance while it would increase for plateau intrapulmonary pressure to damage to the lung tissue.

웨이브렛 계수 Zero Crossings를 이용한 영상의 잡음제거 및 압축

신재호,이용준,은종숙,이성우 동국대학교 산업기술연구원 1997 산업기술논문집 Vol.10 No.-

윤곽선이나 에지같은 영상 데이터의 중요한 성분들은 웨이브렛 계수의 zero crossings를 통해 검출할 수 있다. 이런 방법은 영상의 패턴 인식에 관한 연구에서 sharp variation points를 검출하는데 사용되었다. 웨이브렛 변환과 zero-crossings를 거쳐 국부 극치의 위치를 검출하는데 이 방법을 적용하여서 영상의 잡음제거에 사용하도록 개선하였다. 더욱이 영상 데이터에서 전체 영상의 픽셀과 정보를 저장하는 대신, 대부분의 중요한 성분을 효과적으로 몇몇 국부 극치값으로 압축하였다. 그 결과 새로 개선된 기법은 패턴 인식 분야에서만 아니라 높은 압축률의 잡음 제거에도 사용될 수 있다. The important image data components such as contours and edges could be detected by zero crossings of wavelet coefficient. This method has been used for the studies on pattern recognition of images to detect sharp variation points. We have applied it to detection of the position of local maxima through zero-crossings and wavelet transform, so that it is improved to be used for noise-eliminating of image. In addition it could compress the image data into a few of local maxima which held the most of important component efficiently without saving All image pixels and information. As the result, the newly improved method could be implemented not merely for the field of pattern recognition but for that of noise removal at the higher compression ratio.

임베디드 시스템을 이용한 홈오토메이션 시스템 설계 및 구현

신종욱,윤병우 경성대학교 공학기술연구소 2004 공학기술연구지 Vol.11 No.-

An embedded home automation system with PXA255 was designed and implemented in this paper. TV receiver system, visitors' image storing and checking system with USB camera are implemented in this system. Fire, gas leaking, and intrusion alarms with several sensors are implemented too. TCLN9081DA27D TV tuner was used for this system and its device driver was implemented for PXA255 embedded system. USB camera was used for capturing of visitors images, and the captured images can be displayed in the TFT LCD monitor. TDUHC124B USB host controller was used for the interfacing between USB camera and implemented embedded system. The captured images from USB camera can be compressed as JPEG and stored as files. The stored images can be displayed in the TFT LCD at any time.

인듀서 내부의 캐비테이션 유동과 흡입성능에 관한 연구

申澈洙,金鍾喆,姜宇政,李先基 충주대 산업과학기술연구소 1995 産業科學論文集 Vol.3 No.-

In order to understand the influence of the inducer inlet angle on cavitation and suction performance, experiments were carried out for two kinds inducer, which have the same outlet angle distribution, length and number of inducer blades. The results showed that the change of inducer inlet angles influenced not only internal flow conditions but also cavity flow and suction performance. The improvement of suction performance by using inducer were also discussed.