산채류 추출물이 세균의 성장에 미치는 영향(Ⅰ)

지원대,이종만,정영건 효성여자대학교 부설 한국환경위생연구소 1994 환경위생연구 Vol.4 No.1

본 실험은 일반 가정의 식탁에서 흔히 볼 수 있는 산채류 20종에 대해 3주의 gram 양성균(Bacillus subtilis, Lactobacillus casei, Staphylococcus aureus)및 2주의 gram음성균(Klebsie-lla pneumoniae, Shigella sp.)의 성장에 미치는 영향을 조사하였다. 본 실험에 사용한 대부분의 산채류 추출액은 공시균의 성장을 다소 증가시켰다. 특히 표고버섯과 고사리는 그람양성균인 B.subtilis, L.casei, S.aureus균의 취, 더덕과 토란은 B.subtilis, S. aureus균의 성장을 대조군에 비해 2~6배로 크게 증가시켰다. 그러나 그람음성균인 K.pneumoniae와 Shigella sp.에 대해서는 성장을 크게 증가시키는 시료는 없었다. 한편, 죽순은 B.subtilis의, 부지깽이와 민들레는 L.casei의, 팽이버섯과 돌나물 및 두릅은 S. aureus의, 산초, 다래순, 두릅 및 마는 K. pneumoniae의, 죽순, 두릅 및 마는 Shigella sp.의 생육을 다소 저해하는 것으로 나타났으나 항균력은 인정할 수 없었다. This study was carried out to investigate how to influence mountainvegetables, which are commonly available in high mountains in Korea, on bacte-rial growth. Total 20 kinds of mountain vegetables and mushrooms were sampledand after boiling them in water, put water extracts of them into the media prepa-red for the smear of test strains, which were included three of Gram positivestrains (Bacillus subtilis, Lactobacillus casei and Staphylococcus aureus) andtwo of Gram negative strains(Klebsiella pneumoniae and Shigella sp.). The gro-wth of all strains were more or less increased in the media injected with waterextracted samples than that of the control. Especially the growth of B. subtilis,L. casei and S. aureus were increased by the water extracts of Pyogo mushroomand Bracke and the samples from Aster, Deodeok(Codonopsis lanceolata) andTaro were appeared with the increase in growth of B, subtilis and S. aureusby two to six times increase rate than that of control. However, the more growthincrease of K. pneumoniae and Shigella sp. were not found than that of control.There were also observed some of water extracted samples have slight inhibi-tions to the bacterial growth. The growth of strains inhibited by the sampleswere as follows .B. subtilis by water extracts of Bamboo shoot, L. casei bythem of Busiggaengi(Erysimum aurantiacum) and Dandelion, S. aureus by themof Pengi mushroom, Sedum and Fatsia, K. pneumoniae by of them of Chinesepepper, Darae(Actinidia arguta) , Fatsia and Yam and Shigella sp. by of themof Bamboo shoot. Fatsia and Yam. However. these antibiotic effects were soweak that scarecely proved their effects.

혈액투석 환자에게서 발생한 재생불량성 빈혈 1예

정지용,윤나라,강대웅,오종찬,장재현,선길홍,박경희,정종훈,김현리 朝鮮大學校 附設 醫學硏究所 2005 The Medical Journal of Chosun University Vol.30 No.1

Aplastic anemia is a disease characterized by pancytopenia in the peripheral blood and hypocellular bone marrow with the fatty infiltration. The causes of the acquired aplastic anemia were usually related to drugs and immune-related diseases. A 34-years old man was admitted due to general weakness. He has been going hemodialysis for 2 years because of end stage renal disease (ESRD) due to chronic glomerulonephritis. At admission, laboratory tests showed hemoglobin 6.2 g/dL, hematocrit 18.0 %, WBC 5,710mm (neutrophil 71.8%, lymphocyte 19.1%, monocyte 5.9%), reticulocyte 1.9%, platelet 93,000/mm, Fe 12.8 ug/dL, TIBC 204 ug/dL, ferritin 941.47 ng /ml, haptoglobin 0.72 g/L, vitamin B12 508.17 pmol/L, folate 24 ng/mL, total protein 6.54 g/dL, albumin 3.76 g/dL, Alk. pohsphatase 79 IU/L, AST 30.0 IU/L, ALT 39.7 IU/L, total bilirubin 0.57 mg/dl, BUN 79.4 mg/dl and creatinine 10.18 mg/dl. Peripheral blood smear showed the normocytic normochromic anemia with anisocytosis. Anemia was diagnosed but it was irresponsive to the recombinant human erythropoietin (EPO) and blood transfusion. In the bone marrow aspiration smear were there the hypoplastic myeloid and erythroid precursors. The bone marrow biopsy section showed the hypoplasia of all components (10-20%) and the fatty infiltration. We have experienced one case of the idiopathic aplastic anemia in a patient going through hemodialysis and we just report it with documentary records. 저자들은 혈액 투석 중인 환자에서 약물, 면역계 이상등의 원인을 발견할 수 없었음에도 재생 불량성 빈혈이 발생한 1예를 경험하여 문헌 고찰과 함께 보고하는 바이다.

말기신부전 환자에서 혈액투석 전후의 QT간격분산 비교

강대웅,정지용,윤나라,안치용,김종오,신병철,정종훈,김현리 朝鮮大學校 附設 醫學硏究所 2006 The Medical Journal of Chosun University Vol.31 No.2

Background: Some cases of QT interval, corrected QT interval (QTc), QT dispersion (QTd) and corrected QT dispersion (QTcd) have been reported in ESRD, but these results are variable and the influence of a hemodialysis is still controversial, In this study, we investigated the effect of hemodialysis on QT and QTc interval and QT and QTc dispersion in patients with ESRD 15 min before and 15 minute after each hemodialysis. Methods: Sixty-seven patients with ESRD (men 33 & women 34) on three-times stable hemodialysis (>3 months) were randomly enrolled. Fifty control subjects with a similar age and normal renal function were enrolled from this hospital. Routine biochemical studies were measured pre- and post dialysis, at the time of the ECG. Plasma Na+, K+, BUN, creatinine, ionized calcium and phosphate were checked. Twelve-lead electrocardiographs were performed at 10㎜/mv and 50㎜/s using a HewIett-Packard Pagewriter 100, before and after a single hemodialysis session.The QT interval was measured from the onset of the QRS complex to the end of the T wave. If the end of the T wave was not clear in a particular lead then it was excluded from analysis. When U waves were present, the end of the T wave was taken as the nadir between the T and U waves. Each QT interval was corrected for heart rate using Bazett's formula (QTc==QT/√(RR)) (ms). The difference between maximal and minimal QT interval duration was defined as QT dispersion (QTd) in each of the 12 leads. Result: This study demonstrates that QT, QTd, QTcd is higher in hemodialysis patients compared with control subjects, and QT and QTd rise postdialysis to levels comparable to those seen acutely following myocardial infarction, when patients are at greatly increased risk of potentially fatal ventricular arrhythmias, Conclusions: QT interval and QT dispersion, markers of risk for arrhythmias and sudden death, are elevated in hemodialysis patients, and rise postdialysis. QT interval and QT dispersion is an easily obtainable, noninvasive, simple, inexpensive, and widely available method of risk stratification in uremic patients receiving chronic dialysis. Additional studies are needed to clarity whether increased postdialysis QT dispersion results in an increased occurrence of arrhythmias.

香氣療法에 사용되는 精油의 本草學的 接近

박지현,김종대,강석봉 동서의학사 2001 동서의학 Vol.26 No.3

항결핵 치료과정에서 호전되었다가 악화된 뇌 결핵종 1예 보고

이대훈,유진홍,강미자,김홍기,김선화,고동훈,천지성,유원종 대한화학요법학회 1997 대한화학요법학회지 Vol.15 No.3

In spite of nationmide tuberculosis control program, tuberculosis is still prevalent in Korea. Accordingly, tuberculous meningitis also poses one of the main trouble some problems. Recently, We experienced a perplexing case of tuberculous menin-gitis and tubercdoma in a 24-year old female patient. She was admitted to our hospital owing to meningeal irritation sign, aphasia, VI nerve palsy, and ,fever. CSF analysis showed a finding consistent with that of presumptive tuberculous meningitis.. Initial MRI of the brain revealed a large mass(tuhercu1oma) on left parieto-temporal area. Antitubeculous regimen(1soniazid (INH), rifampin(RMP), ethambutol(EMB), pyrazinamide(PZA)} of meningeal dose was given immediately, and her symptoms and signs were dramatically disappeared with a few days after treatment. She was discharged with improved condition and under maintenance treatment of tuberculosis. Follow-up one year after treatment. revealed that the previous lesion was nearly diminished. She showed no symptom at all until she recognized a total blindness of sudden onset on her left eye a t the 18th month of treatment. Brain MRI checked at that time showed multiple masses of tuberculoma scattered over the brain base and one of them invaded left optic nerve. In our opinion, the cause of relapse in this patient might he explained by the selection and ultimate replication of a minor population of resistant strain. Although such relapse is an extremely rare event, we think that we should always pay attention to this possible resistance problem every time we meet patients with tuberculous meningitis.

새로운 열방성 액정중합체의 합성과 PET와의 블렌드

조정대,김희종,최재곤,조병욱,유지강 조선대학교 생산기술연구소 1997 生産技術硏究 Vol.19 No.1

Blends of new thermotropic liquid crystalline polymer(TLCP) with poly(ethylene terephthalate)(PET) were prepared by the coprecipitation from a common solvent. The thermal, mechanical properties and morphology of the blends(2wt%, 5wt%, lOwt% and 20wt% TLCP/PET) were examined. In the blends, liquid crystalline phase did not reveal any significant macrophase separation and thermal degradation at the processing temperature. According to the scanning electron micrograps, the TLCP domains in matrix were found to be more or less finely dispersed with 0.2㎛ to 0.4㎛ in size and interfacial adhesion between the TLCP and matrix polymer was excellent. Results of mechanical property measurement show that modulus of TLCP/PET blend was enhanced with increasing TLCP content.

老人 低血壓에 對한 文獻的 考察

郭益勳,김종대,鄭智天 동국대학교 한의학연구소 1995 東國韓醫學硏究所論文集 Vol.4 No.-

This study was perfomed to investigate causes of the senile hypotension, pathogenic mechanism, symptoms, and therapies through medical literatures, recent chinese medical literatures and chinese medical journals. The results are as follows ; 1. The senile hypotension has major symptoms dizziness, weakness, syncope, palpitation, shortness of breath, and deficiency of Qi. Additionally, it has minor symtoms of letharhy, isomnia, tinnitus, amnesia etc... 2. The prodromal symptoms of Kwul and Kwul are relating to the symptoms of tachycardia, facial pallor, sweating, anxietas, ambiguous consciousness, and fainting. Weakness and dizziness due to deficiency make the symptoms of exhaustion, fatigue, vertigo, lethargy, and brachycardia. 3. The most principal cause of the senile hypotension is deficiency of Shen due to aging, congenital deficiency, and chronic illness. The rest of causes are deficiency of Qi and blood, phlegm of retention, stagnation of Qi, blood stasis, blood prostration etc..In the view of the occidental medicine, the causes of the senile hypotension came from the reduction of cardiac output, the decretion of cardiovascular syatem's extention due to aging, hereditary factor, secondary factor due to exsanguination, diabetes mellitus, C.V.A. etc‥, and factor of neurogenic system's degeneration. 4. The principal pathogenic mechanisms are the insufficiency of Xing-Yang the deficiency of Qi in middle jiao, and deficiency of Shen-Oi. The rest of mechanisms are the deficiency of both Qi and blood, stagnation of the Gan-Qi, and the deficiency of Gan and Shen. Zang-Fu Organs have something to do with Xing, Bi, and Shen. 5. As principal therapies, there are warming and recuperation the Xing-Yang, strengthing the middle-jiao and replenishing Qi, replenishing vital essence to tonify the Shen, and warming and recuperation the Shen-Yang. Additionally, the therapies of invigorating the Qi and relieving menta1 stress, strengthning the Bi and tonifing the Shen, invigorating Qi and nourishing Yin, soothing the Gan and regulating the circulation of Qi, and tonifing the Shen and nourishingthe San help the cure of the senile hypotension. In prescriptions there are Baohe Yuan Tang, Buzhong Yigi Tang, Zuoguei Yin, Yougui Yin, Guipi Tang, Zhu Fu Tang, Shengmai San, Sini San, and Qi ju Dihuang Wan. The medical herbs of Astragali Radix, Codonopsitis Pilosulae Radix, Ginseng Radix, Aconiti Tuber, Ephedrae Herba, Cinnamomi Ramulus, Cinnamomi Corfex Spissus, Zingiberia Rhizoma, Polygalae Radix, Liriopis Tub er, Polygonati Sibirici Rhizoma, Lycii Fructus, Schigandrae Fructus and Glycyrrhizae Radix can be treated. 6. According to the clinical report, the principal causes are the deficiency of Qi, and insufficiency of Yang which symptoms are dizziness, vitality fatigue and acratia, amnesia, body cold and alger of 'extremity, spontaneous perspiration, and therapy and weak pulse. It was improved by taking WenYang YiQi Tang, Zhu Fu Tang about 20-30 days. The improvement was shown on disappearance of subjective symptoms or the ascending of blood pressure to normal figure, and the rate of improvement was over 70% . 7. As regimens, taking warming and recuperating food(a sheep mutton, juglans regia, chiness date, longan aril ete‥) and pungent food(chinese green onion, fress ginger, pipers fructns etc‥), doing physical training, not being ill in bed at a long time, and preventing descent of blood pressure coming from sudden change of posture are needed. Additionally, the usage of diuretic, abirritant, and depressor needs to be extra cautious.

운동 강도의 차이가 흰쥐의 혈액 성분과 적혈구의 형태에 미치는 영향

김부환,김종오,지용석,최대원,윤진환,정일규,김영욱,오봉석 한국스포츠리서치 2004 한국 스포츠 리서치 Vol.15 No.4

This study was to investigate the effects on hematological indices and heinz body formation in various intensity, Male Sprague-Dawley rats were randomly divided into the control group, the low-intensity exercise group, nigh-intensity exercise group (n=7 in each group). Animals of the low-intensity exercise groups were put on exercise 30 min per 1 day, 5 days per week for 6-weeks. Animals of the high-intensity exercise groups were put on exercise 120 min per 1 day, 5 days per week for 6-weeks. Red blood cell count (RBC), hemoglobin (Hgb), hematocrit (Hct), mean ceil volume (MCV), mean hemoglobin corpuscular (MCH), mean hemoglobin corpuscular concentration (MCHC) were analyzed in automated hematology analyzer (ABX Hematology MICROS 60) from 10㎕ of whole blood. Erythrocyte morphology were evaluated in super vital (Brilliant cresyl blue stain) stained blood films. In the present results, Hg, MCHC in the high-intensity exercise group was significantly decreased compared with the different exercise intensity and the control group. MCV in the high-intensity exercise group was significantly increased compared with the different exercise intensity end the control group. Heinz body formation in the high-intensity exercise group was significantly increased compared with the different exercise intensity and the control group. In the present study, it can be suggested that intensity of exercise can influence hematological variables and heinz body formation. These findings suggest that high intensity exercise induces oxidative damage to erythrocyte.

1,1,1,2-Tetrafluoroethane(HFC-134a)과 Propane(HC-290)의 2성분계 기-액 평형 측정

진현상,박지영,이병권,양대륙,임종성 한국화학공학회 2004 Korean Chemical Engineering Research(HWAHAK KONGHA Vol.42 No.2

혼합물을 냉매로 사용할 경우, 냉매의 성능 평가를 위해 기-액 상평형 데이터가 필수적으로 요구된다. 본 연구에서는 HFC-134a와 propane의 이성분계 상평형 데이터를 얻기 위해 273.15, 283.15, 283.15, 303.15, 313.15, 323.15K의 조건에서 실험을 실행하였다. 상평형 실험 장치로는 circulation type의 장치를 사용하였다. 실험데이터는 Peng-Robinson-Stryjek-Vera(PRSV)상태방정식과 NRTL excess Gibbs free energy model을 결합한 식에 다섯 개의 혼합법칙(Huron-Vidal original and modified equation)들을 이용하여 상관관계를 알아보았다. PRSV 상태방정식을 이용하여 얻은 데이터는 실험 데이터와 만족할 만한 일치를 볼 수 있었다. 실험은 실행한 모든 온도에서 공비점이 형성하는 것을 확인하였다. Vapor-liguid equilibrium(VLE) data are essential when using mixtures as a refrigerant. The VLE data of binary mixture containing 1, 1, 1, 2-tetrafluoroethane and propane were measured at 273.15, 283.15, 293.15, 303.15, 313.15 and 323.15K using circulation type experimental apparatus. The experimental data were correlated with five mixing rules combined with the Peng-Robinson-Stryjek-Vera equation of state. We compared the data obtained by using the PRSV EOS with experimental data and a satisfactory consistency of model predictions with experimental data were obtained. Azeotropic behavior has been observed in all these mixtures.

淸肝散이 急性 Ethanol 中毒에 흰쥐의 肝損傷에 미치는 影響

곽익훈,김종대,정지천,신억섭 한국한의학연구원 1996 한국한의학연구원논문집 Vol.2 No.1

Chunggansan(淸肝散) was tested for the effects on detoxication mechanism of alcohol. Chunggansan was treated firstly into samples, and then ethanol intoxicated animal models were set with them. In vitro, the level of lipid peroxide in tissue of liver proportionally decreased with the level of concentration of extract prepared from Chunggansan. In vivo, after the extract was administered to the animal model for seven days, the level of lipid peroxide in liver dependently decreased in proportion of concentration. The glutathione percentage purposely induced acute ethanol intoxicated samples, followed by Chunggansan pre-medicating administration, increased in compare with the group treated with only ethanol. Also, the enzyme activities of ALT, AST, and r-GTP in liver decreased.