정신분열병에 대한 리스페리돈의 효과 및 안정성

이민수,김용구,김영훈,연병길,오병훈,윤도준,윤진상,이철,정희연,강병조,김광수,김동언,김명정,김상훈,김희철,나철,노승호,민경준,박기창,박두병,백기청,백인호,손봉기,손진욱,양병환,양창국,우행원,이정호,이종범,이홍식,임기영,전태연,정영조,정영철,정인과,정인원,지익성,채정호,한상익,한선호,한진희,서광윤 大韓神經精神醫學會 1998 신경정신의학 Vol.37 No.1

연구목적 : 본 시험의 목적은 임상시험 시작전에 연구자들을 대상으로 PANSS Workshop을 통하여 PANSS, ESRS에 대한 국내에서의 표준화 작업을 구축하고 새로운 정신병 치료제인 리스페리돈의 효과와 안정성을 재확인하여 리스페리돈 사용에 대한 적정화를 이루는데 있다. 연구방법 : 1996년 4월부터 1996년 9월까지 국내 39개 대학병원 정신과에 입원중인 혹은 증상이 악화되어 입원하는 정신분열병 환자 377명을 대상으로 다시설 개방 연구를 시행하였다. 1주일간의 약물 배설기간을 가진후, 리스페리돈을 8주간 투여하였고, 기준점, 1주, 2주, 4주, 그리고 8주후에 평가되었다. 용량은 제1일에는 리스페리돈 1mg씩 1일 2회, 제2일에는 2mg씩 1일 2회, 제3∼7일에는 3mg씩 1일 2회 투여하였다. 이후 환자의 임상상태에 따라 임의로 증량할 수 있으며, 최대 일일 16mg을 초과하지 않도록 하였다. 추체외로 증상을 조절하기 위한 투약을 허용하였다. 임상증상 및 부작용의 평가는 PANSS(Positive and Negative Syndrome Scale), CGI(Clinical Global Impression) 그리고 ESRS(Extrapyramidal Symptom Rating Scale)을 사용하였다. 연구결과 : 377명중 343명(91%)이 8주간의 연구를 완결하였다. 치료 종결시점인 8주후 PANSS 총점수가 20% 이상 호전된 경우를 약물 반응군으로 정의할때, 약물반응군은 81.3%였다. 리스페리돈에 반응하는 예측인자로는 발병연령, 이전의 입원 횟수, 유병기간이 관련 있었다. 리스페리돈은 1주후부터 PANSS양성, 음성, 및 일반정신병리 점수상에 유의한 호전을 보여 효과가 빨랐다. CGI의 경우도 기준점에 비해 1주후부터 유의한 감소를 나타내었다. ESRS의 경우, 파킨슨 평가점수는 기준점과 비교해 투여 1주, 2주, 4주후 유의하게 증가되었다가 8주후 기준점과 차이가 없었다. Dystonia 평가점수는 1주후만 유의한 증가를 보였으며, dyskinesia 평가점수는 유의한 차이가 없었다. 혈압, 맥박수의 생명징후 및 일반 혈액학 검사, 생화학적 검사, 심전도 검사에서 유의한 변화는 없었다. 결 론 : 이상의 다시설 개방 임상 연구를 통해 리스페리돈은 정신분열병 환자에서 양성증상뿐만 아니라 음성증상 및 전반적인 증상에도 효과적인 것으로 사료된다. 보다 명확한 평가를 위해서는 다른 항정신병약물과의 이중맹검 연구가 필요할 것으로 생각되며, 또한 장기적 치료에 대한 평가도 함께 이루어져야 하겠다. Objective : The purpose of this study was to investigate the efficacy and safety of risperidone in the treatment of Korean schizophrenic patients. Method : This multicenter open study included 377 schizophrenic patients drawn from 39 university hospitals. After a wash-out period of 1 week, the schizophrenic patients were treated with risperidone for 8 weeks and evaluated at 5 points ; at baseline, and 1, 2, 4 and 8 weeks of treatment. The dose was increased from 2mg/day(1mg twice daily) to 6mg/day(3mg twice daily) during the first week and adjusted to a maximum of 16mg/day over the next 7 weeks according to the patient's clinical response. Medication to control extrapyramidal symptoms was permitted. The psychiatric and neurological status of the patients was assessed by PANSS, CGI, and ESRS scales. Results : 343(91%) of 377 patients completed the 8-week trial period. Clinical improvement, as defined by a 20% or more reduction in total PANSS score at end point, was shown by 81.3% of patients. The predictors of response to risperidone were associated older age, shorter duration of illness, fewer previous hospitalization. Risperidone had rapid onset of action ; a significant decrease of the total PANSS and three PANSS factor(positive, negative, general), and CGI was already noticed at the end of first week. For the ESRS, parkinsonism rating scores were significantly increased until week 4 comparing with baseline. Dystonia rating scores were significantly increased until week 1, and dyskinesia rating scores were not significantly changed during the study. Laboratory parameters including vital sign, EKG, hematological, and biochemical values showed no significant changes during the trial. Conclusions : This study suggests that risperidone is generally safe and effective against both the positive and negative symptoms in our group of patients.

수종의 구강 함수제가 치은 섬유아세포에 미치는 영향

서조영,박준봉 慶北大學校 齒科大學 1990 慶北齒大論文集 Vol.7 No.1

세균성 치태의 축적을 경감시키는 목적으로 구강함수제로 사용되는 0.1%와 1% Chlorhexidine, Cetylpyridinium Chloride과 Listerine이 치은 섬유아세포에 미치는 영향을 규명하여 이들 약제들의 생물학적 평가를 도모하고자 시험관적 실험을 실시하였다. 위 약제성분들이 함유된 구강함수제를 구입하여 소독후 100배 희석액을 만들었다. 치은 조직으로부터 분리 배양한 섬유아세포를 60mm 배양접시에 넣어 24시간 전 배양후 상충액을 제거한 다음 준비된 배양액을 재 주입히킨후 24, 48, 72시간동안 37℃ 100 습도 5% Co^2공기 혼합 배양기에서 배양하였다. 배양접시 기저부에 부착된 세포를 trypsin으로 분리하여 그 수를 측정하여 다음과 같은 결과를 얻었다. 시간이 경과함에 따라 세포수의 변화는 Listerine에서는 약간의 감소를 보였으며, 0.1% CHX, CPC, 1% CHX에서는 현저히 감소하였다. 치은 섬유아세포에 대한 독성은 Listerine, 0.1% CHX, CPC, 1% CHX 순으로 강하게 나타났다. 순수 배양액만으로 배양한 경우 24시간까지는 세포가 증식하지 않았으며, 72시간까지는 약2배의 세포 증식을 보였다. The purpose of this study was to evaluate the effect of the four gargling solutions-Listerine, Cetylpyridium chloride(CPC), 0.1% % 1% Chlorhexidine(CHX)-on the human gingival fibroblasts in vitro. The 3×10 exp (5) cells of human gingival fibrobalsts were seeded in each 60mm Petri dish with Dulbecco's Modified Eagle's Medium containing 100u/ml penicillin, 100㎍. ml streptomycin, 1㎍/ml amphotericin-B and 10% fetal bovine serum and incubated for 24 hours. After discard of the supernatant of old medium, those cells cultured with fresh prepared medium at 37℃ 5% Co2/air incubator. The number of cells in the each dish were counted on the hemocytometer after 24, 48, 72 hours incubation. The results were as follows; 1. In the course of time, Listerine was the weak decrease of cell counts and 0.1% CHX, CPC & 1%CHX were the most significant decrease of cell counts in comparision to control cultures. 2. To the cytotoxic effect on the human gingival fibroblast, Listerine, 0.1%CHX, CPC, 1%CHX in order showed more cytotoxic. 3. In the case of culture medium only used, the number of cells were increased two times at 72 hours after seeding. J. Kyungpook Univ. Sch. Dent. Vol. 7, No. 1, 1∼8, 1990.

Agrobacterium transformation 에 의해 Manganese SOD가 과발현된 transgenic tobacco의 개발에 대한 연구

徐廷彬,金榮照,李宇成 성균관대학교 기초과학연구소 1995 論文集 Vol.46 No.2

식물체는 냉해, 가뭄, air pollutants 등과 같은 다양한 외부 환경적인 스트레스에 노출되어짐으로써 매우 활동적인 oxygen radicals 생성을 유도한다. 이러한 활동적인 oxygen radicals 는 식물체 내의 수 많은 생리적 문제를 유발시킨다. 이러한 스트레스에 적응하고자 식물체 내에는 다양한 방어 기작이 있으며, 그 중 하나가 superoxide를 Hydrogen peroxide로 바꾸는 효소인 superoxide dismutase (SOD, EC 1.15.1.1)이다. 방어 기작에 있어서 SOD의 역할을 조사해 보고자, 본 실험에서는 담배의 엽록체에 완두의 manganese SOD를 과발현시킨 형질 전환 식물체를 개발하였다. Northern hybridization과 SOD activity gel staining을 해 본 결과, 이러한 형질 전환된 담배 엽록체 내에서 완두의 manganese SOD가 안정적으로 발현되고 있음을 확인할 수 있었다. Upon exposure of plant to diverse environmental stress such as chilling, drought, and air pollutants, the activated oxygen radicals are actively produced. These reactive oxygen species are associated with a number of physiological disorders in plant. One of the enzymes involved in the defense is superoxde dismutase (SOD, EC 1.15.1.1) which converts superoxide into hydrogen peroxide. To test the role of SOD in the defense system, We produce transgenic plants which are overexpressed Mn SOD of pea (Pisum sativum L.) in chloroplasts of tobacco (Nicotiana tabacum L. cv Xanthi). Using Northern hybridization and SOD activity gel staining, it was confirmed that these transgenic plants overexpressed the introduced gene.

PDGF와 IGF-I 병용 사용시 치주인대세표의 증식과 세포활성에 미치는 영향에 관한 연구

서조영,신홍인,경희문 경북대학교 병원 1997 경북대학교병원의학연구소논문집 Vol.1 No.1

Current acceptable methods for promoting periodontal regeneration are based on removal of diseased soft tissue, root treatment, guided tissue regeneration, introduction of new graft materials and biological mediators. Insulin-like growth factor-I(IGF-I) and Platelet-derived growth factor-BB(PDGF-BB), the members of the polypeptuyde growth factor family have been reported as the biological mediators which regulate a variety cellular matrix biologic activities of wound healing process including the cell proliferation, migration and extracellular matrix synthesis. The purposes of this study is to evaluate the combination effects of IGF-I and PDGF-BB on the cellular activity of the periodontal ligament cells to act as a regeneration promoting agent of periodontal tissue. Human periodontal ligament cells were prepared from the first permolar tooth extracted for the orthodontic treatment and were cultured in DMEM containing 10% FBS at the 37。C, 5% CO2 incubator. Author measured the DNA synthetic activity, and total protein, collagen and noncollagenous protein synthetic activities according to the concentration of 10, 100ng/ml IGF-I and 1, 10 ng/ml PDGF-BB in combination. The results were as follows: significantly increased in the 1 ng/ml PDGF-BB alone compared to the 10 ng/ml PDGF-BB alone(P<0.01)and in the 1 ng/ml PDGF-BB AND 10, 100NG/ML IGF-I in combination compared to the 1ng/ml PDGF-BB alone (P<0.05,P<0.01). The synthetic activity of the noncollagenous protein is increased according to the concentration of IGF_I, but not statistically significant(P>0.05). The percent of collagen is significantly in the 1ng/ml PDGF-BB and 10ng/ml IGF-I in combination compared to the 1ng/ml PDGF-BB alone(P0.05) and in the 10ng/ml IGF-I in combination compared to the 10ng/ml PDGF-BB alone(P<0.05). The synthetic activity of the DNA is in conclusions, the percent study shows that PDGF-BB and IGF-I in combination have a potentiality to enhance the DNA synthesis and the total protein and collagen synthesis of the periodontal ligament cells, especially it is more significant in the low concentration PDGF-BB compared to the high one. Thus, the PDGF-BB and IGF-I in combination may have important roles in promotion of periodontal ligament healing, and consequently, may useful for clinical application in periodontal regenerative procedures.

의복소비 및 활용실태 분석

趙必嬌,徐英淑,具銀鈴 服飾文化學會 1997 服飾文化硏究 Vol.5 No.2

폐농양 및 복부농양을 일으킨 Eikenella corrodens 감염 2예 : Two Case Reports and Review

정용희,류미숙,김영식,이우인,이희주,서환조 대한화학요법학회 2000 대한화학요법학회지 Vol.18 No.1

Eikenella corrodens is a slowly growing, facultative anaerobic gram-negative bacillus. It is a normal inhabitant of the human upper respiratory tract, and it is recognized as an infrequent cause of invasive disease. To date, a few cases of E. corrodens infection were noted from all other organ. We report herein two cases of E. corrodens infection. In one case E. corrodens was isolated in the lung abscess and in the other case it was in the appendiceal abscess. The patients were treated empirically with penicillin G. The results of the bacterial culture and sensitivity test were available with it. The patients were discharged 15 days later with marked improvement after treatment. Our report clearly demonstrates that E. corrodens can be the pathogen of lung abscess and intraabdominal abscess. We reviewed descriptions from the literature of infections caused by E. corrodens.

품질보증수준확보를 위한 최적검사설비대수의 결정

송서일,조영찬 동아대학교 공과대학부설 생산기술연구소 1998 生産技術硏究所硏究論文集 Vol.3 No.1

As the rapid growth of industrial society, many enterprises are inclined to 100 ppm management, even more to ppm management from the traditional 3σ management method. Therefore, it needs to extend process capability index for guarantee of high quality assurance level to ppm unit. Thus, in this paper, using the probability of standard normal distribution from 3σ tp 6σ, which was developed by an author, the optimal numbers of inspection facility is determined for guarantee of process capability index and quality assurance level. Also, high quality assurance level is determined by considering the precision of inspection when one and two sided specification is given for the probability which is out of the specification limit.

말라리아 환자 32예의 임상적 고찰

정용희,백일현,김태형,김영식,이우인,서환조 대한화학요법학회 2000 대한화학요법학회지 Vol.18 No.1

목적 : 삼일열 말라리아는 국내에서 1986년 이후에는 박멸된 것으로 알려졌으나 1993년 이후 증가하는 양상을 보이고 있다. 이에 말라리아의 임상적 고찰을 통하여 이 질환을 이해함에 있다. 방법 : 1991년 1월부터 1999년 10월까지 반복적 고열과 오한을 주소로 본 병원에 내원하여 말초혈액 도말검사에 의해 말라리아 감염으로 확진된 32예의 환자에 대해서 후향적으로 조사하였다. 결과 : 1991년부터 1999년 10월까지 총 32명의 환자의 연도별 발생빈도는 각각 1991년에 1명, 1996년에 1명, 1997년에 3명, 1998년에 13명, 1999년에 14명이었다. 월별발생빈도는 7월과 8월에 집중적으로 발생하였다.(46.9%) 환자의 거주지는 경기 연천과 파주에 주로 분포되었다(53.1%). 성별발생분포는 32예의 환자중에 여자가 5명, 남자가 27명으로 남자가 더 많았다. 발병연령은 22세에서 87세까지 분포되었고 20-30대에 많이 발생하였으며 20대가 가장 많았다. 군인 및 제대자가 17명(59.4%)에 해당하였으며, 제대자의 평균 제대후의 기간은 10개월 정도였다. 가장 흔한 임상증상은 발열 및 오한이었다.(100%) 비장종대 및 간장종대는 각각 26명(81.3%), 3명(9.4%)에서 관찰되었다. 검사실 소견은 평균적으로 WBC 5.317/㎕, 혈색소 12.5±7.1g/㎗, 혈소판 78,906.3±68391.4㎕, GOT/GPT 43.7±26.7/49.6±57.3 IU/ℓ, Total bilirubin 1.6±0.9㎎/㎗를 보였다. 말초혈액 도말검사상 모두 P. vivax였다. 치료는 hydroxychloroquine과 primaquine의 치료에 잘 반응하였다. 결론 : 말라리아는 7월과 8월에 한국에서 호발하는 중요한 급성 열성 질환으로 조기 진단 및 치료에 보다 관심을 기울여야 할 것이다. Background : In Korea malaria has been rare, but since reemergence of indigenous vivax malaria in 1993, the number of cases of malaria have recently increased. We analyzed the cases of malaria who were treated in Kyung-Hee University College of Medicine with regard to define the clinical characteristics of malaria. Methods : We retrospectively analyzed of 32 cases of malaria admitted to Kyung-Hee University Hospital between 1991 and 1999. Malaria was diagnosed by peripheral blood smear. Results : Among a total of 32 cases, the number of yearly incidence of 1991, 1996. 1997, 1998 and 1999 were 1,1,3,13,14 respectively. It demonstrated the high incidence especially between July and August. The most patients (53.1%) resided in Yeonchon and Pajoo. Among them, 5 cases were female and 27 cases were male. The age distribution ranged from 22 to 76 year old. The high incidence of age was between 20's and 30's. Military solidiers and ex-soldiers are 17 cases(59.4 %). The most frequent symptom was fever and chills (100%). Splenomegaly and hepatomegaly were also observed in 81.3 %(26 of 32) and 9.4 %(3 of 32) respectively. The laboratory findings were WBC 5.317/㎕. Hb 12.5±2.1 g/dl. platelet 78,906±68,391.4/㎕. GOT/GPT 43.7±26.7/49.6±57.3 IU/L. LD 678.3±50.1 IU/L. total bilirubin 1.6±0.9mg/dl- The all cases were treated suscessfully with hydroxychloroquine and primaquine. There were no central nervous system complications and pulmonary edema. Conclusion : Malaria is one of the important acute febrile illness with the high incidence between July and August.

치은 섬유아세포에 미치는 치주포대 추출물의 영향

이덕혜,서조영,박준봉 慶北大學校 齒科大學 1990 慶北齒大論文集 Vol.7 No.1

The purpose of this study was to evaluate the effect of soluble extracts form four peridontal dressings, two of which were eugenol and the others were non-eugenol, upon human gingival fibroblasts in vitro. Each of mixed dressing which added to medium was incubated in the 35mm petri dish for 24hrs. After discard of the supernatant of old medium, those cells were cultured with fresh prepared medium in the 5% CO2 incubator at the state of 37℃, for 24, 48, & 72 hours. Author counted the number of cells by hemocytometer at each designed time and observed the change of morphology of cells stained with Hematoxylin-Eosin on the inverted phase contrast microscope The results were as follows. 1. In the course of time, K.P pack was the most significant decrease of cell counts in comparison to control cultures with Wondrpak, Coe-pak Cellopack following in that order at original extracts and there were somewhat decrease at 1/10 & 1/100 diluted solutions in all samples. 2. Throughout the present study, the eugenol containing periodontal dressings, K.P pack & Wondrpak, showed more cytotoxic effects on fibroblasts than non-eugenol dressings, Cellopack & Coe-pak. 3. In microscopic apearance of four dressings, the degenerative changes with pyknotic nuclei, lysis of cytoplasm and marked vacuolizaion were evident at orignal extracts. 4. At 1/10 & 1/100 diluted solutions, there was nothing remarkable morphological change in all kinds of periodontal dressings as time goes by. J. Kyungpook Univ. Sch. Dent. Vol. 7, No. 1, 9∼21, 1990

종합검진센터 내원자에서 관상동맥질환 위험요인들의 분포

이충원,이종영,박종원,윤능기,김영조,이현우,이무식,서석권 啓明大學校 醫科大學 1993 계명의대학술지 Vol.12 No.3

1991년3월부터 8월사이에 대구시에 소재한 1개의 대학병원의 종합검진센타에 내원해서 종합검진을 받은 남자 422명(69.9%)과 여자 182명(30.1%)을 대상으로 하여 심헐관계질환의 위험요인들의 전체적인 분포를 보았다. 평균 연령은 남자가 43.3(표준편차, 10.3), 여자가 44.4(표준편차, 10.8)였다. 남자에서 연령에 따라 0.05 수준에서 통계적인 유의성을 보인 위험인자는 혈청 총콜레스테롤(TC). 트리글리세라이드(TC), 고밀도 지단백콜레스테롤(HDL), 저밀도 지단백콜레스테롤(LDL), 수축기혈압, 비체중, A형행동양상이었으며 생활습관으로서는 음주, 흡연, 규칙적인 운동, 우유와 커피섭취, 수면 등이었다. 여자에서는 TC, TG, LDL, 수축기와 확장기혈압, 비체중 그리고 커피섭취와 수면 등이었다. 남자에서 총콜레스테롤의 평균은 181.7mg/dl(표준편차, 32.2), 여자는 182.5mg/이(표준편차, 42.2)였다. 대부분의 위험인자들은 구미의 수준에 미치지 못했으나 남자에서 흡연율이 전체적으로 61.8%로 높으 수준이었으며 특히, 20~29세가 72.9%, 30~39세가 75.2%로서 다른 연령군에 비해 상대적으로 높았다. 여자에서는 전체적으로 6.0%에 지나지 않았다. 확장기혈압 90mmHg 이상을 고혈압자로 정의했을 때의 유병률이 여자 전체대상자에서 24.7%로 높았으며 50-59세는 표본수가 적어서 문제가 되었으나 53.1%였다. 어떤 인구집단내에서 총콜레스테롤의 평균이 200mg/dl 미만이면 고혈압과 흡연의 인구집단의 수준에 관계없이 관상동맥질환(coronary heart disease)의 발생은 드문 것으로 보고가 되고 있으므로 당분간 관상동맥질환 발생률의 급속한 절대적인 증가는 힘드리라 사료된다. 그러나 이러한 결과는 본 연구의 대상자들이 대표성을 지니지 못하며 건강검진센터에 자발적으로 내원한 사람들이므로 해석에 주의를 요한다. Authors examined the distributions of the risk factors for the coronary heart disease in the 422 male (69.9%) and 182 female (30.1%) visitors to the health examination center of a university hospital located in Taegu March to August 1991. Mean age of males was 43.3(standard deviation, SD 10.3) and that of females was 44.4(SD 10.8). Total cholesterol(TC), triglyceride (TG), high-density lipoprotein and low-density lipoprotein cholesterol(LDL), systolic blood pressure, Quetelet index, and Type A Behavior Pattern, and some of life habit variables(alcohol intake, smoking, regular exercise, milk and coffee intake and sleeping) were statistically significant in age groups of the males(P<0.05). In the females, TC, TG and LDL, systolic and diastolic pressure, Quetelet index, coffee intake and sleeping were statistically significant in age groups (P<0.05). Mean of total cholesterol was 181.7㎎/dl(SD,32.2) in males and that of females was 182.5㎎/dl(SD,42.2). Most of the risk factors levels were lower than the Euro-Americans', while smokers were high with 61.8%, especially 72.9% in 20-29 age group and 75.2% in 30-39 age group in males. In females, smokers were just 6.0%. Hypertensives defined by more than 90mmHg diastolic pressure were 24.7% in females, particularly 53.1% in 50-59 age group, but size of the strata was rather small(N=49). In the light of the report that coronary heart disease is uncommon irrespective of population levels of smoking and hypertension, where average total blood cholesterol level in a population is low(<200mg/dl), it is not likely that the absolute increase of the number of the coronary heart disease will increase markedly in the near future. But cautions should be exercised in interpreting the results of this study due to the lack of representativeness and volunteerism. .