Combined transcriptomic and proteomic analysis of flubendiamide resistance in Plutella xylostella

Li Jing‐Jing,Jin Ming‐Hui,Wang Nian‐Meng,Yu Qi‐Tong,Shang Ze‐Yu,Xue Chao‐Bin 한국곤충학회 2020 Entomological Research Vol.50 No.10

Diamondback moth (DBM), Plutella xylostella, is an important pest of crucifers worldwide. The extensive use of diamide insecticides has led to DBM resistance in the world, and this presents a serious threat to vegetable production. In the present study, transcriptomic and proteomic analyses were combined to investigate the potential flubendiamide‐resistance mechanism in DBM. The lab‐selected (Rh) and field‐collected (Rb) flubendiamide‐resistant lines of P. xylostella with resistance ratios of 1889.92‐fold and 1250.97‐fold, respectively, were used, as well as a lab‐reared flubendiamide‐susceptible line (S). Compared with the S group, the transcriptomic analysis revealed 151 upregulated and 287 downregulated gene messengers in the Rh group and 432 upregulated and 565 downregulated gene messengers in the Rb group. The most frequently enriched pathways of differentially expressed genes (DEGs) were mainly involved in metabolic pathways. Metabolism related genes, including two P450, two ABC transporters, and three trypsins, were upregulated in the Rh line. Additionally, some P450 genes, trypsin, juvenile hormone (JH), and mucin genes were also upregulated in the Rb line. In proteomic analysis comparisons with the S group, there were 78 upregulated and 90 downregulated proteins in the Rh group and 221 upregulated and 155 downregulated proteins in the Rb group. Further analyses found that three CYP and 11 CYP proteins were over‐expressed in Rh and Rb lines, respectively. Four glutathione S‐transferase (GST) and four UGTs were over‐expressed in Rb line. So, we deduced that the detoxification metabolism may be the main mechanism of flubendiamide resistance in P. xylostella.

Electro-hydraulic coupling jet printing technology based on Weissenberg effect under pulse voltage

Jing Wang,Xintao Wu,Wei Wang,Bing Xu,Yuanjie Fang,Jing Tang 대한기계학회 2021 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.35 No.6

In this paper, electro-hydraulic coupling jet printing technology based on the Weissenberg effect is put forward for the first time, and the pulse voltage is used to realize the on-demand printing. The experimental results show that the Weissenberg effect can inhibit the solution reflux and make the solution converge into a cone tip at the nozzle, which is more conducive to position printing precisely. By exploring the influence principles of process parameters on printing, it is found that droplet diameter is positively correlated with rotation speed and negatively correlated with voltage frequency. The printing frequency is negatively correlated with the rotation speed and positively correlated with the voltage frequency. However, the effect of voltage amplitude and duty ratio on the printing effect is not obvious in a certain range. It is found that the rotation speed of needle core within the range of 1800 r/min-7500 r/min can print out the ideal lattice structure, which proves the feasibility of this system to realize on-demand positioning printing. Finally, by using the printing equipment, the sacrificial layer structure of PDMS microfluidic chip was successfully made and the function of the microfluidic chip was realized, which further verified the practicability of the technical scheme proposed in this paper.

Adverse effect of DEHP exposure on the serum insulin level of Balb/c mice

Jing Wang,Jinquan Li,Kashif Rafiq Zahid,Kun Wang,Yan Qian,Ping Ma,Shumao Ding,Xu Yang,Xianliang Wang,Xianliang Wang,Xu Yang 대한독성 유전단백체 학회 2016 Molecular & cellular toxicology Vol.12 No.1

Di(2-ethylhexyl) phthalate (DEHP) is a common indoor pollutant in the world, which may cause lots of harmful effects in human including diabetes according to epidemiological studies. To explore the underlying role of DEHP in diabetes-like symptoms, Balb/c mice were chose to be the experimental animals in this paper. They were separated as eight groups as follows: (1) saline+normal diet (vehicle control), (2) 10 mg/kg.day DEHP+normal diet, (3) 50 mg/kg.day DEHP+normal diet, (4) 250 mg/kg.day DEHP+normal diet, (5) streptozotocin (STZ)+high fat diet (diabetes model), (6) 10 mg/kg.day DEHP+ STZ+high fat diet, (7) 50 mg/kg.day DEHP+STZ +high fat diet, (8) 250 mg/kg.day DEHP+STZ+high fat diet. The biomarkers of this experiment include four groups: (1) general indicates: body weight and drinking water, (2) blood biomarkers: serum insulin and fasting glucose, (3) pathological examination: pancreas section and kidney section, and (4) biomarkers of oxidative stress: reactive oxygen species (ROS) and malondialdehyde (MDA) in liver cells. Our study results demonstrate that: (1) at our treatment levels DEHP cannot directly induce diabetes, but reduce serum insulin level in DEHP-exposed non-STZ-treated animals, (2) pathological examination finds that these is a dose-dependent damage in the pancreas in DEHP- exposed STZ-treated groups, and (3) the oxidative mechanism may be involved in this pathological process.

Minimum Bandwidth Regenerating Codes Based on Cyclic VFR Codes

( Jing Wang ),( Shuxia Wang ),( Tiantian Wang ),( Xuefei Zhang ) 한국인터넷정보학회 2019 KSII Transactions on Internet and Information Syst Vol.13 No.7

In order to improve the reliability and repair efficiency of distributed storage systems, minimum bandwidth regenerating (MBR) codes based on cyclic variable fractional repetition (VFR) codes are constructed in this thesis, which can repair failed nodes accurately. Specifically, in order to consider the imbalance of data accessed by the users, cyclic VFR codes are constructed according to that data with different heat degrees are copied in different repetition degrees. Moreover, we divide the storage nodes into groups, and construct MBR codes based on cyclic VFR codes to improve the file download speed. Performance analysis and simulation results show that, the repair locality of a single node failure is always 2 when MBR codes based on cyclic VFR codes are adopted in distributed storage systems, which is obviously superior to the traditional MBR codes. Compared with RS codes and simple regenerating codes, the proposed MBR codes based on cyclic VFR codes have lower repair locality, repair complexity and bandwidth overhead, as well as higher repair efficiency. Moreover, relative to FR codes, the MBR codes based on cyclic VFR codes can be applicable to more storage systems.

Prediction Models for Solitary Pulmonary Nodules Based on Curvelet Textural Features and Clinical Parameters

Wang, Jing-Jing,Wu, Hai-Feng,Sun, Tao,Li, Xia,Wang, Wei,Tao, Li-Xin,Huo, Da,Lv, Ping-Xin,He, Wen,Guo, Xiu-Hua Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.10

Lung cancer, one of the leading causes of cancer-related deaths, usually appears as solitary pulmonary nodules (SPNs) which are hard to diagnose using the naked eye. In this paper, curvelet-based textural features and clinical parameters are used with three prediction models [a multilevel model, a least absolute shrinkage and selection operator (LASSO) regression method, and a support vector machine (SVM)] to improve the diagnosis of benign and malignant SPNs. Dimensionality reduction of the original curvelet-based textural features was achieved using principal component analysis. In addition, non-conditional logistical regression was used to find clinical predictors among demographic parameters and morphological features. The results showed that, combined with 11 clinical predictors, the accuracy rates using 12 principal components were higher than those using the original curvelet-based textural features. To evaluate the models, 10-fold cross validation and back substitution were applied. The results obtained, respectively, were 0.8549 and 0.9221 for the LASSO method, 0.9443 and 0.9831 for SVM, and 0.8722 and 0.9722 for the multilevel model. All in all, it was found that using curvelet-based textural features after dimensionality reduction and using clinical predictors, the highest accuracy rate was achieved with SVM. The method may be used as an auxiliary tool to differentiate between benign and malignant SPNs in CT images.

Exploration on the Development and Characteristics of Composites Painting in the Contemporary Art Ecology

Wang Jing Jing 국제문화기술진흥원 2023 International Journal of Advanced Culture Technolo Vol.11 No.1

The ecological expression of art in modern society takes the harmonious developmental relationship between man and nature as an element and combines it with the various manifestations of painting as an expression of ecological artistic development. The necessary relationship for the harmonious development of nature is accurately articulated, and the ecological changes in people are expressed in integrated materials that inspire human reflection. In the pursuit of material pleasures, mankind is neglectful and indifferent to the environment. The development of composite painting in art and ecology is a process that more reveres the harmony between man and nature as well as satisfying the creative value of the work. After systematic evaluation and research, people have engaged in various structural forms of composite art painting development in the long history of art development, focusing on the integration of environmental and ecological culture.In the process of nature education going through development, the comprehensive practical development of nature education is enhanced and efforts are made to feel new ecological art ideas and new ways of valuing environmental protection. In this paper, an observational study of eco-art will be carried out, starting from the theory of hierarchical division and analyzing the contradictory relationship between man and nature. Recognize nature, understand it and feel it through eco-art painting. The analysis of the contradictory relationship between man and nature is combined with the identification of various types of information that give value to the environmental protection public, and the combination of ecological painting and nature art to achieve an upgrade of the environmental protection idea of the value of painting. In the development of artistic ecological comprehensive painting, it is necessary to put forward technical requirements and standards that meet the development of modern industry according to the characteristics and methods of ecological painting. The author of this paper discusses the necessity and social value of artistic ecological painting creation from the artist's point of view, expounds the practical achievements of ecological painting from the perspective of the history and present situation of the development of ecological aesthetics at home and abroad, and gives the concept and creative method of contemporary art comprehensive material painting in combination with his own actual situation.

중국 수출통제법 내용분석과 대응방안

왕정정(Jing-Jing Wang),주선(Xuan Zhou),최창환(Chang-Hwan Choi) 한국통상정보학회 2020 통상정보연구 Vol.22 No.4

본 연구는 2020년 12월 발효될 ｢중화인민공화국 수출통제법｣에 대해 초안과 달라진 점과 기본적 입법 취지를 파악하는 한편, GATT 제XXI조(예외조항), 미국 무역확장법 제232조 조치와 차이점 및 우리 기업에 대한 시사점을 제시하였다. 수출통제법 연구결과 제9조 수출통제리스트, 제12조 Catch all 의무조항, 13조 허가기준에 국가안전과 이익 등을 근거로 수출을 통제할 수 있으나, 국가안전과 이익의 개념이 모호할 뿐만 아니라 GATT/WTO 안보 예외 사항에 해당하여 중국이 이 조항을 근거로 자의적으로 수출통제를 할 위험성이 매우 높다고 할 것이다. 수출통제법 발효로 인한 수출통제 대상과 범위, 처벌 수위가 높아질 것으로 예상되며, 이에 따라 중국진출 우리 기업들의 연관된 제품 수출시 동 법률을 자세히 사전 검토할 필요가 있으며, 수입하는 우리 기업들도 수출통제 대상이 되지 않도록 품목, 대상, 최종 사용자 등에 관한 철저한 연구가 선행되어야 할 것이다. This study examines the differences from the draft and the final version of the “Export Control Law of the People s Republic of China”, which will take effect in December 2020, while comparing the differences in Article XXI of GATT, Section 232 of the U.S. Trade Expansion Act, and its implications for Korean businesses. As a result of the study of the Export Control Act, exports can be controlled based on national safety and interests in accordance with Article 9 Export Control List, Article 12 Catch All obligations clause, and Article 13 Permit Standards, but the concept of national safety and interests is ambiguous. In addition, it would be said that there is a very high risk that China will arbitrarily control exports based on this provision as it falls under the GATT/WTO security exception. It is expected that export control targets, scope, and penalties will increase due to the entry into force of the Export Control Act. Accordingly, it is necessary to carefully review the law when exporting related products by Korean companies entering China. Thorough research on items, targets, and end users should be preceded so that they do not become targets.

The Positive Association between Subclinical Hypothyroidism and Newly-Diagnosed Hypertension Is More Explicit in Female Individuals Younger than 65

Xichang Wang,Haoyu Wang,Li Yan,Lihui Yang,Yuanming Xue,Jing Yang,Yongli Yao,Xulei Tang,Nanwei Tong,Guixia Wang,Jinan Zhang,Youmin Wang,Jianming Ba,Bing Chen,Jianling Du,Lanjie He,Xiaoyang Lai,Yanbo Li 대한내분비학회 2021 Endocrinology and metabolism Vol.36 No.4

Background: Subclinical hypothyroidism (SCH) is the most common thyroid dysfunction, and its relationship with blood pressure(BP) has been controversial. The aim of the study was to analyze the association between SCH and newly-diagnosed hypertension. Methods: Based on data from the Thyroid disease, Iodine nutrition and Diabetes Epidemiology (TIDE) study, 49,433 euthyroid individuals and 7,719 SCH patients aged ≥18 years were enrolled. Patients with a history of hypertension or thyroid disease were excluded. SCH was determined by manufacturer reference range. Overall hypertension and stage 1 and 2 hypertension were diagnosedaccording to the guidelines issued by the American College of Cardiology/American Heart Association in 2017. Results: The prevalence of overall hypertension (48.7%), including stage 1 (28.9%) and 2 (19.8%) hypertension, increased significantly in SCH patients compared with euthyroid subjects. With elevated serum thyroid stimulating hormone (TSH) level, the hypertension prevalence also increased significantly from the euthyroid to different SCH subgroups, which was more profound in femalesor subjects aged <65 years. The age- and sex-specific regression analysis further demonstrated the same trends in the general population and in the 1:1 propensity matched population. Similarly, several BP components (i.e., systolic, diastolic, and mean arterial BP)were positively associated with TSH elevation, and regression analysis also confirmed that all BP components were closely relatedwith SCH in female subjects aged <65 years. Conclusion: The prevalence of hypertension increases for patients with SCH. SCH tends to be associated with hypertension and BPcomponents in females younger than 65 years.

Fotemustine, Teniposide and Dexamethasone in Treating Patients with CNS Lymphoma

Wu, Jing-Jing,Wang, Xin-Hua,Li, Ling,Li, Xin,Zhang, Lei,Sun, Zhen-Chang,Fu, Xiao-Rui,Ma, Wang,Chang, Yu,Zhang, Xu-Dong,Han, Li-Juan,Zhang, Ming-Zhi Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.11

Purpose: We developed and evaluated a regimen including fotemustine, teniposide and dexamethasone (FTD) for treating patients with central nervous system (CNS) lymphoma based on pharmacokinetic properties of individual agents and in combination. Patients and Methods: In a comparison study, 8 patients with primary CNS lymphoma (PCNSL) and 8 with secondary CNS lymphoma (SCNSL) were treated with FTD (comprising fotemustine 100 mg/m2, 1h infusion, day 1; teniposide 60 mg/m2, >0.5 h infusion, on day 2, 3, 4; dexamethasone 40 mg, 1h infusion, on day 1, 2, 3, 4 and 5; and methotrexate 12 mg, cytosine arabinoside 50 mg plus dexamethasone 5 mg intrathecally, on day 2 and 7). Cycles were repeated every 3 weeks. After response assessment, patients received whole brain radiotherapy. Results: Of the 8 PCNSL patients, 4 (50%) achieved CR and 3 (38%) PR, an overall response rate of 88%. Four patients (50%) were in continuing remission at the end of this study after a median follow-up of 30 months (range 10 to 56 months). Of the 8 SCNSL patients the overall response rate was 63% (CR+PR: 38%+25%). All responses were achievable with predictable toxicity mainly reflecting reversible myelosuppression. Conclusion: This study suggests that FTD could be an effective treatment for CNS lymphoma, and is worthy of further evaluation.

Adaptive-and-Resolvable Fractional Repetition Codes Based on Hypergraph

Tiantian Wang,Jing Wang,Haipeng Wang,Jie Meng,Chunlei Yu,Shuxia Wang 한국인터넷정보학회 2023 KSII Transactions on Internet and Information Syst Vol.17 No.4

Fractional repetition (FR) codes can achieve exact uncoded repair for multiple failed nodes, with lower computational complexity and bandwidth overhead, and effectively improve repair performance in distributed storage systems (DSS). The actual distributed storage system is dynamic, that is, the parameters such as node storage overhead and number of storage nodes will change randomly and dynamically. Considering that traditional FR codes cannot be flexibly applied to dynamic distributed storage systems, a new construction scheme of adaptive-and-resolvable FR codes based on hypergraph coloring is proposed in this paper. Specifically, the linear uniform regular hypergraph can be constructed based on the heuristic algorithm of hypergraph coloring proposed in this paper. Then edges and vertices in hypergraph correspond to nodes and coded packets of FR codes respectively, further, FR codes is constructed. According to hypergraph coloring, the FR codes can achieve rapid repair for multiple failed nodes. Further, FR codes based on hypergraph coloring can be generalized to heterogeneous distributed storage systems. Compared with Reed-Solomon (RS) codes, simple regenerating codes (SRC) and locally repairable codes (LRC), adaptive-and-resolvable FR codes have significant advantages over repair locality, repair bandwidth overhead, computational complexity and time overhead during repairing failed nodes.