Expression and Significance of TSGF, CEA and AFP in Patients Before and after Radical Surgery for Colon Cancer

Hu, Yi,Wang, Jing-Liang,Tao, Hai-Tao,Wu, Bai-Shou,Sun, Jin,Cheng, Yao,Dong, Wei-Wei,Li, Rui-Xin Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.6

Objective: To explore the expression and significance of tumor specific growth factor (TSGF), carcinoembryonic antigen (CEA) and alpha fetoprotein (AFP) in cancer tissue and serum of patients with colon cancer. Materials and Methods: Radical surgery for colon cancer was performed on 43 patients with laparoscopu under conditions of general anesthesia. The Elisa method was used to detect the levels of serum TSGF, CEA and AFP before and after radical operation, and cancer tissue underwent TSGF, CEA and AFP immunohistochemistry staining after laparoscopic surgery. The decreased conditions of serum TSGF, CEA and AFP in patients with colon cancer at different levels of differentiation and clinical stagings were analyzed, and the relationships of expression rates between histological types, colon cancer morphology, lymph node metastasis and TSGF, CEA as well as AFP in cancer tissue were assessed. Results: Compared with before radical surgery, the levels of serum TSGF, CEA and AFP decreased notably in patients after operations (p<0.01). The decreased degree of TSGF and CEA was the largest in patients with poorly differentiated cancer tissue (p<0.01), while that of AFP was noted in patients with moderately differentiated cancer tissue (p<0.01). The decreased degree of TSGF and AFP was the largest in patients at phase Dukes A (p<0.01), while that of CEA in patients at phase Dukes C (p<0.01). There were no significant differences among the positive expression rates of TSGF, CEA and AFP with different histological types and colon cancer morphologies (p>0.05). The positive expression rates of TSGF and CEA in patients with lymph node metastasis were significantly higher than those without lymph node metastasis (p<0.01). Conclusions: TSGF, CEA and AFP can be used to evaluate the effect of radical operation for colon cancer, and the changed levels of different markers are associated with tumor differentiation, clinical stating and presence or absence of lymph node metastasis.

Analysis of Peripheral B Cell Subsets in Patients With Allergic Rhinitis

Jing Luo,Huanhuan Guo,Zhuofu Liu,Tao Peng,Xianting Hu,Miaomiao Han,Xiangping Yang,Xuhong Zhou,Huabin Li 대한천식알레르기학회 2018 Allergy, Asthma & Immunology Research Vol.10 No.3

Purpose: Recent evidence suggests that B cells can both promote and inhibit the development and progression of allergic disease. However, the characteristics of B cell subsets in patients with allergic rhinitis (AR) have not been well documented. This study aimed to analyze the characteristics of B cell subsets in the peripheral blood of AR patients. Methods: Forty-seven AR patients and 54 healthy controls were enrolled in this study, and the B cell subsets in peripheral blood of all subjects were analyzed by flow cytometry. Moreover, the serum total immunoglobulin E (IgE) and IgE concentrations secreted into the cultured peripheral blood mononuclear cells (PBMCs) were measured by using enzyme-linked immunosorbent assay. Results: We found the peripheral blood of AR patients contained higher percentages of memory B cells, plasma cells, and CD19+CD24hiCD27+ regulatory B cells (Bregs) than those of age-matched healthy controls (P<0.05), while the percentages of naïve B cells and CD19+CD24hiCD38hi Bregs were significantly lower in AR patients than in healthy individuals (P<0.05). In addition, the serum total IgE and IgE concentrations secreted into the cultured PBMCs were elevated in AR patients than in the healthy controls (P<0.05). Conclusions: Our findings indicate that AR patients were characterized by increase in terminally differentiated memory B cells or plasma cells and decreases in CD19+CD24hiCD38hi Breg cells in the peripheral blood.

Lactate potentiates angiogenesis and neurogenesis in experimental intracerebral hemorrhage

Jing Zhou,Tao Liu,Hao Guo,Hanjin Cui,Pengfei Li,Dandan Feng,En Hu,Qing Huang,Ali Yang,Jun Zhou,Jiekun Luo,Tao Tang,Yang Wang 생화학분자생물학회 2018 Experimental and molecular medicine Vol.50 No.-

Lactate accumulation has been observed in the brain with intracerebral hemorrhage (ICH). However, the outcome of lactate accumulation has not been well characterized. Here, we report that lactate accumulation contributes to angiogenesis and neurogenesis in ICH. In the first set of the experiment, a rat model of ICH was induced by injecting collagenase into the brain. The effects of lactate accumulation on the neurological function, apoptosis, and numbers of newborn endothelial cells and neurons, as well as the proliferation-associated signaling pathway, were evaluated in the rat brain. In the second set, exogenous L-lactate was infused into intact rat brains so that its effects could be further assessed. Following ICH, lactate accumulated around the hematoma; the numbers of PCNA+/vWF+ nuclei and PCNA+/DCX+ cells were significantly increased compared with the numbers in the Sham group. Moreover, ICH induced translocation of nuclear factor-kappa B (NF-κB) p65 into the nucleus, resulting in a notable upregulation of VEGF and bFGF mRNAs and proteins compared with the levels in the Sham controls. Administration of a lactate dehydrogenase inhibitor dramatically inhibited these effects, decreased the vascular density, and aggravated neurological severity scores and apoptosis after ICH. After exogenous L-lactate infusion, the numbers of PCNA+/vWF+ nuclei and PCNA+/DCX+ cells were strikingly increased compared with the numbers in the Sham controls. In addition, lactate facilitated NF-κB translocation to induce increased transcription of VEGF and bFGF. Co-infusion with an NF-κB inhibitor significantly inhibited these effects. These data suggest that lactate potentiates angiogenesis and neurogenesis by activating the NF-κB signaling pathway following ICH.

Synthesis and characterization of monodisperse yttrium aluminum garnet (YAG) micro-crystals with rhombic dodecahedron

Xu, Tao,Yuan, Rui,Xu, Peng-Cheng,Pan, Dong-Jie,Yang, Woochul,Guo, Hai-Bo,Shen, Yu-Fang,Hu, Jian-Feng,Zhang, Zhi-Jun,Zhao, Jing-Tai Elsevier 2018 Journal of alloys and compounds Vol.762 No.-

<P><B>Abstract</B></P> <P>Uniform, monodisperse yttrium aluminum garnet (YAG) rhombic dodecahedron micro-crystals were synthesized using coprecipitation and hydrothermal methods. The Na<SUB>2</SUB>SO<SUB>4</SUB> as a surfactant was helpful in improving the dispersity of the crystallites and forming well-faceted, micro-sized dodecahedral YAG crystallites. The effect of the reaction time and the solvent has also been studied. The crystal growth mechanism called dissolution/crystallization was determined by analyzing the experimental and simulated results. And the specific well-developed crystal facets in the {110} family have been demonstrated by the theoretical calculation. A higher integrated emission intensity can be achieved for the YAG:Ce crystallites by improving the phase purity, morphology with better developed dodecahedrons, and dispersion. Furthermore, a rather high luminous efficacy (LE = 104.14 lm/W) for the white LED fabricated using the YAG:Ce<SUP>3+</SUP> phosphor prepared in this work was realized. The correlation between the crystal growth, morphology and luminescence properties of YAG crystallites has been established, which exhibits a great importance for the synthesis and application of mono-dispersed YAG crystallites.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Uniform and monodisperse YAG rhombic dodecahedron micro-crystals were synthesized. </LI> <LI> The growth mechanism of YAG rhombic dodecahedron was studied through experimental and computational simulation. </LI> <LI> The correlation between the crystal growth, morphology and luminescence of YAG crystallites was established. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

Bevacizumab Concomitant with Chemotherapy is Effective in Treating Chinese Patients with Advanced Non-Squamous Non-Small Cell Lung Cancer

Sun, Jing,Hu, Yi,Wu, Bai-Shou,Wang, Jin-Liang,Tao, Hai-Tao,Zhang, Su-Jie Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.14

Objectives: To retrospectively review the safety and clinical efficacy of bevacizumab concomitant with chemotherapy in Chinese patients with advanced non-squamous non-small cell lung cancer (NSNSCLC). Methods: Clinical data for 79 patients with NSNSCLC who received bevacizumab concomitant with chemotherapy in Chinese PLA General Hospital from April 28th 2009 to May 5th 2013 were retrospectively reviewed to analyze the clinical efficacy including disease control rate (DCR), overall response rate (ORR), progression-free survival (PFS), overall survival (OS), the Eastern Cooperative Oncology Group (ECOG) score and the safety. Results: The Eastern Cooperative Oncology Group (ECOG) score was 0-2. By the final cutoff date (June 9, 2013), 54 (68.4%) patients had disease progression and 37 (46.8%) died. The ORR was 32.9% and the DCR was 83.5%. The ORR of the first-, second-, and third- or later-line treatments were 51.4%, 25.0% and 12.5%, while the DCR were 94.3%, 80.0% and 70.8%, respectively. The median OS (mOS) and PFS (mPFS) were 13.5 and 5.83 months, respectively. The mOS of patients with the first-, second-, and third- or later- line treatments were 16.2, 10.9 and 8.30 months, while the mPFS were 7.27, 5.90 and 5.17 months, respectively. Chemotherapy-related adverse events included myelosuppression, vomiting, hepatic dysfunction and renal dysfunction, while the common serious bevacizumab-related adverse events were thromboembolic problems, gastrointestinal perforation and reversible posterior leukoencephalopathy syndrome, which could be well managed. Conclusions: Bevacizumab concomitant with chemotherapy is effective and the related toxicity can be well tolerated in Chinese patients with NSNSCLC.

Highly selective separation of vanadium over iron from stone coal by oxalic acid leaching

Pengcheng Hu,Yimin Zhang,Tao Liu,Jing Huang,Yizhong Yuan,Qiushi Zheng 한국공업화학회 2017 Journal of Industrial and Engineering Chemistry Vol.45 No.-

In this paper, a novel method was proposed to selectively extract vanadium from stone coal by oxalicacid leaching. Vanadium was leached, but the iron impurity wasmaintained in the leaching residue. Theeffects of leaching conditions on the leaching efficiency of vanadium and iron were investigated foroxalic and sulfuric acid leaching, respectively. For oxalic acid leaching, 71.5% of the vanadium can berecovered with only 3.4% of the iron impurity leached under the leaching conditions: a dosage of H+ of12 mol/kg, a leaching time of 6 h, a leaching temperature of 368 K (95 8C) and a water–mineral ratio of1.5 L/kg. However, under the same conditions, 74.1% of the vanadium and 13% of the iron were leachedduring sulfuric acid leaching. The XRD and FTIR analyses showed that both oxalic and sulfuric acidleaching can destroy the crystal lattice structure of muscovite, resulting in the leaching efficiency ofvanadium without much difference. Further, the SEM-EDS and XPS analyses indicated that very littlepyrite dissolved during oxalic acid leaching, but that much more pyrite dissolved during sulfuric acidleaching. Therefore, oxalic acid leaching can achieve a highly selective separation of vanadium over ironfrom stone coal.

Low-temperature insertion bonding using electroless Cu-Co-P micro-cones array with controllable morphology

Yaqian Sun,Jing Wang,Xundi Zhang,Chenlin Yang,Anmin Hu,Tao Hang,Yunwen Wu,Huiqin Ling,Ming Li 대한금속·재료학회 2021 ELECTRONIC MATERIALS LETTERS Vol.17 No.6

At present, thermal compression bonding based on Cu and lead-free Sn based solder is often limited by high bonding temperature,which is higher than the melting point of solder (218 ℃). In this paper, we reported a low-temperature solid stateinsertion bonding method based on electroless Cu-Co-P micro-cones array. By adjusting the mass ratio of CuSO 4 ·5H 2 O andCoSO 4 ·7H 2 O, a series of Cu-Co-P micro-cones with diff erent morphologies were prepared. The Cu-Co-P micro-cones withhigher proportion of copper were sharper and denser and (111) orientation was also more. It was found that reducing theheight and density of micro-cones was conducive to achieve seamless bonding at lower temperature and force such as 170℃ and 750 gf. By optimizing the morphology of micro-cones, such as height, bottom diameter, vertex angle and density, theseamless and reliable bonding with high shear strength (39.9 MPa) could be achieved at 170 ℃ bonding temperature and1000 gf bonding force. The transmission electron microscopy results showed that intermetallic compounds including Cu 6 Sn 5and Cu 3 Sn existed at bonding interface, which indicated that signifi cant atomic diff usion had occurred between Cu-Co-Pmicro-cones and Sn based solder. Probable mechanisms for low-temperature insertion bonding were discussed.

An Improved Discontinuous Deformation Analysis to Solve Both Shear and Tensile Failure Problems

Wenjun Gong,Jing Hu,Zhigang Tao 대한토목학회 2019 KSCE JOURNAL OF CIVIL ENGINEERING Vol.23 No.5

This paper is mainly aimed to present an improved DDA (discontinuous deformation analysis) that can deal well with both shear and tensile failure problems. Firstly, the shear mechanism of DDA is detailed investigated. The results show that when handling the frictional interface, the critical shear resistance can be accurately determined only if the penalty value is carefully selected, however, when handling the cohesive interface, the critical shear resistance is significantly underestimated. The inaccurate prediction is due to the inconsistent distribution of normal force and shear force between the two vertex-to-edge contacts in one edge-to-edge contact. Here an edge-to-edge treatment is introduced into DDA. Secondly, to moderately reflect the tensile failure process of rock masses, a two-phase constitutive model is introduced into the DDA with edge-to-edge treatment, and the improved DDA is obtained. Finally, the improved DDA is used to simulate the failure process of gypsum centrifuge model. The results show the improved DDA can deal well with rock failure problems by shear or tension failure.

Transcriptomic analysis of developing embryos of apricot (Prunus armeniaca L.)

Yujia Bai,Weicheng Hu,Min Wang,Jing He,Yongxia Tao,Wei Huang,Zuoshan Feng 한국원예학회 2016 Horticulture, Environment, and Biotechnology Vol.57 No.2

Apricot (Prunus armeniaca L.) is an important, nutritionally valuable agricultural crop. A scarcity of transcriptomic and genomic data hinders progress toward understanding the molecular events of apricot embryogenesis, which is the primary determinant of fruit yield and kernel quality. In this study, three cDNA libraries were sequenced using an Illumina/Solexa platform. We obtained 35,928,496, 40,656,940, and 35,970,214 clean reads from endosperm formation (LT-A), embryo development (LT-B), and mature embryo (LT-C) cDNA libraries, respectively. De novo assembly of cDNA sequence data generated 35,614 unigenes with an average length of 973 bp. A total of 29,971 unigenes were matched in Nr, Nt, Swiss-Prot, KEGG, GO, and COG databases. The top matching species in the Nr database was P. persica (88.3% of matched Nr database accessions). A total of 8,327 differentially expressed genes (DEGs) were detected between LT-A and LT-B, of which 3,663 were upregulated and 4,664 were downregulated. Between LT-B and LT-C, 12,673 DEGs were detected, while 13,892 DEGs were uncovered between LT-A and LT-C. This work is the first reported application of next-generation sequencing techniques for transcriptome analysis of apricot embryo development. Besides providing valuable information on apricot embryo development, the comprehensive set of transcript sequences generated in this study may serve as additional molecular resources for the development of apricot functional genomics.

Analysis on Survival and Prognostic Factors for Cancer Patients with Malignancy-associated Hypercalcemia

Zhang, Su-Jie,Hu, Yi,Cao, Jing,Qian, Hai-Li,Jiao, Shun-Chang,Liu, Zhe-Feng,Tao, Hai-Tao,Han, Lu Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.11

Objective: To explore the incidence, clinical characteristics, diagnosis and treatment strategies, prognosis of patients with malignancy-associated hypercalcemia (MAH). Methods: The data of 115 patients with MAH who were treated at the Medical Oncology Department of Chinese PLA General Hospital from Jan., 2001 to Dec., 2010 was retrospectively reviewed. Survival analysis was performed using the Kaplan-Meier method and the Cox proportional hazard model with statistic software SPSS 18.0. Results: The patients had blood calcium levels ranging from 2.77 to 4.87 mmol/L. Except for 9 cases who died or were discharged within 5 days after admission, all other patients recovered to normal blood calcium level after treatment with bisphosphonates or intravenous hydration and diuretics; their survival after occurrence of MAH was from 1 day to 4,051 days, and the median survival time was only 50 days. In the log-rank test, the male, renal metastasis, central nervous system symptoms and hypercalcemia occurring over 140 days after cancer diagnosis were predictors of poor survival (P=0.002, P=0.046, P=0.000, P=0.009). In the COX analysis, being male, central nervous system symptoms and hypercalcemia lasting over 140 days after cancer diagnosis were independent prognostic factors for survival time (RR=2.131, P=0.027; RR=3.054, P=0.002; RR=2.403, P=0.001). According to these factors, a score system was established to predict the patient prognosis and adjust the treatment. Conclusion: Cancer patients with MAH have an extremely poor median survival. Some independent factors indicate poor prognosis, including male gender, central nervous system symptoms and hypercalcemia lasting over 140 days after cancer diagnosis. The prognostic score can serve as a reference for MAH prognosis and treatment, worthy of further investigation.