Interaction between Brain-Derived Neurotrophic Factor Val66Met Polymorphism and Recent Negative Stressor in Harm Avoidance

Kim, Seog Ju,Cho, Seong-Jin,Jang, Ha Min,Shin, Jonghan,Park, Pil-Whan,Lee, Yu Jin,Cho, In Hee,Choi, Jung-Eun,Lee, Heon-Jeong S. Karger AG 2009 Neuropsychobiology Vol.61 No.1

<P>There have been controversial results regarding the association between brain-derived neurotrophic factor (BDNF) Val66Met polymorphism and anxiety-related traits such as harm avoidance (HA). We aimed to investigate the interaction between <I>BDNF</I> Val66Met polymorphism and negative life stressors in HA. <I>BDNF</I> Val66Met polymorphism was genotyped in 391 community-dwelling Koreans (152 males, 239 females; 43.2 ± 14.1 years old). The Temperament and Character Inventory (TCI) and the Center for Epidemiological Studies for Depression Scale (CES-D) were self applied. The Structured Clinical Interview for DSM-IV axis I disorders and face-to-face interviews investigating negative life stressors within the last 6 months were also performed. There was no significant difference in TCI score, major depressive disorder prevalence and CES-D score among the 3 genotypes (94 Met/Met, 188 Val/Met and 109 Val/Val subjects). There was no significant difference in TCI scores between subjects with stressors and those without stressors, while more common major depressive episodes (p = 0.03) and higher CES-D scores (p < 0.001) were found in subjects with stressors. However, there was a significant interaction between the <I>BDNF</I> genotype and negative life stressors in HA (p = 0.02). Only subjects with the Val/Val genotype showed higher HA with recent negative stressors. Our finding suggests that <I>BDNF</I> Val66Met polymorphism might influence HA by interacting with recent negative stress experience.</P><P>Copyright © 2009 S. Karger AG, Basel</P>

광섬유상의 TiO2-코팅조건에 따른 이소프로필 알코올의 광분해

유동식,하진욱 한국공업화학회 2005 응용화학 Vol.9 No.1

The photodegradation of IPA according to coating conditions was examined by TiO₂/P-25-coated. POF. The following results can be concluded. In the photoactivity of TiO₂-coated POF, ethanol solvent was higher than other solvents. Inorganic(KR-400), organic(A-9540) and inorganic-organic hybrid(GPTMS·TMOS) resins were used as binder. TiO₂ coated surface of POF was clear with all binders. Organic binder(A-9540) showed the highest activity for IPA degradation. But organic binder was degraded by TiO₂, inorganic binder had lower binder ability than others. The optimum activity obtained when the ratio of TMOS and P-25 was 0.05~1:1.

일부 인쇄회로기판 납땜근로자들의 눈, 호흡기, 피부의 증상에 대한 조사연구

유재영,우극현,김진석,함정오,최태성,하봉구,정상재,박신구,김일룡 大韓産業醫學會 2001 대한직업환경의학회지 Vol.13 No.4

목적 : 이 연구는 납점작업자를 대상으로 납점작업으로 유발될 가능성이 높은 눈, 호흡기, 피부 등의 증상호소율 및 노출강도에 따른 관련성을 파악하기위해 수행되었다. 방법 : 연구참가자 146명중 연구목적에 적합한 20명을 제외한 126명을 대상으로 '눈이 가렵고 충혈됨', '코가 가렵거나 파가움', '재채기', '콧물'. '코막힘' , '목이 따끔거림' , '목에 무언가가 걸려있는것 같음' , '갑자기 연속적인 기침을 함' , '운동 시에 다른 사람에 비해 숨이 가쁨', '숨을 쉴 때 쌕쌕하는 소리가 남', '가래가 끓음', '손이나 안면에 가려움을 느낌' , '안면에 여드름이 날' '손이나 안면에 붉은 반점이 남' 등 14가지 증상에 대한 경험유무와 혈중연농도, 납점작업의 건강위험요인에 대한 지식상태 등을 조사하였다. 결과 : 조사된 14가지의 증상 중, '갑자기 연속적인 기침을 함'이 하루 납점시간 4시간 이상인 군아서 4시간 미만인 군보다 유의하게 증상호소율이 높았으며, 다른 증상의 경우 하루 납땜시간에 따라 유의한 결과를 보이지 않았다. 납땜작업의 건강위험요인에 대한 질문에 12.8 %가 플럭스에 의한 건강위험을 지적하였다. 전체 대상자의 평균 혈중연농도는6.05 rg/dL였으며, 히고치는 15.50 rg/dL이었다.결론 : 납땜작업자에게 호흡기 증상의 위험이 높을 가능성이 있으며, 납땜작업의 건강위험에 대한추가적인 연구와 납점작업자들을 대상으로 납점작업에 의해 유발될 수 있는 건강장해에 대한 적극적인교육 및 홍보활동을 필요로 한다. Objectives : This study was conducted to evaluate the prevalence of ocular, respiratory and skin symptoms among solderers and to investigate the relationship between symptom prevalence and exposure intensity. Methods : We analyzed 126 eligible participants out of a population of 146 male solder-ers who completed the symptom questionnaires. Fourteen symptoms including 'itchy and red eyes', 'itchy or prickly nose', 'sneezing', 'rhinorrhea', 'blocked nose', 'prickly throat', 'foreign body sensation in throat', 'sudden bouts of coughing', 'exertional breathlessness', 'wheezing', 'sputum production', 'itchy face or hands', 'acneiform erup-tions on the face' and 'red spots on the face or hands' were contained. Blood lead levels of all the 126 participants were tested and the participants' own assessments of the health risk of soldering were collected. Results : Of the 14 investigated symptoms, 'sudden bouts of coughing' was significantly more prevalent in solderers who worked 4 hours or more a day than those who worked less than 4 hours a day, as for the other symptoms, there were no significant differences in the prevalences related to daily soldering hours. 2.8% of the solderers considered the risk of flux exposure to be serious. The mean blood lead bevel was 6.05 μg/dL (maximum 15.50 μg/dL). Conclusions : Soldering may increase the risk of respiratory symptoms. Further invert tigations on the hazards of soldering processes are warranted and solderers should be educarted on these hazards.

금속판상의 TiO2-코팅 조건에 따른 이소프로필 알코올의 광분해

유동식,하진욱 한국공업화학회 2005 응용화학 Vol.9 No.1

The photodegradation of IPA according to coating conditions was examined by TiO₂/'P-25-coated aluminium metal plate. The following results can be concluded. Inorganic(KR-400), inorganic-organic hybrid(TMOS) and organic(A-9540) resins were used as binder. Ti0₂ coated surface of aluminum metal plate was clear with all binders. The activity for IPA degradation appeared to range in order A-9540>TMOS>KR-400. The optimum activity obtained when the ratio of TMOS and P-25 was 1.6:10, and when the ratio of A-9540 and P-25 was 1.0:6.0.

흡연습관의 변화가 혈청지질변화에 미치는 영향

신용하,이종영,김진석,유재영 순천향의학연구소 2003 Journal of Soonchunhyang Medical Science Vol.9 No.1

Objectives : This study was conducted to assess the effect of smoking status change on the lipid profile levels and to construct fundamental data on smoking and health promotion. Methods : 581 male examinees who had taken two times of general health screening tests for aged people from 1996 till 2000 were classified into four groups - continuous non-smoker, continuous smoker, smoking stopper and new smoker and change of serum lipid profile levels between two times of tests among 4 groups were compared. Results : At the first tests, 344(59.2%) out of 581 examinees were smokers and among them, 30(5.2%) stopped smoking while among initial non-smokers, 28(4.8%) began smoking. Low-density lipoprotein cholesterol levels according to smoking status change were +6.8±27.4 for continuous non-smokers, +11.2±25.0 for continuous smokers, +10.8±28.7 for smoking stoppers, and -7.6±22.5 for new smokers (adjested by alcohol intake and obesity index, p=0.002). Differences of triglyceride levels by smoking status changes were -3.9±78.5 for continuous non-smokers, -13.6±89.6 for continuous smokers,+5.5±65.0 for smoking stoppers, +31.4±73.7 (adjusted by alcohol intake and obesity index, p=0.048) Conclusions: Although the results of this study should be interpreted in the restricted sense since there had not been effective controls over important confounding variables that might have been related to serum lipid profile levels, like changes of health belief or behavior, For smoking cessation to be more effective strategy for prevention of hyperlipidemia and atherosclerotic cardiovascular diseases, elevation of lipid profile levels and weight gain following smoking cessation should also be prevented through concomitant dietary modification and sufficient exercise.

외상 환자에서 의식소실 유무와 외상후 스트레스 장애의 유병율, 증상 양상, 우울 및 불안에 관한 연구

이유진,연규월,우행원,김영철,임원정 大韓神經精神醫學會 2002 신경정신의학 Vol.41 No.4

연구목적 : 본 연구에서는 의식 소실 유무에 따른 외상후 스트레스 장애 유병율 및 증상, 우울, 불안 증상을 알아보기 위해 외상을 당한 환자를 의식 소실이 있었던 환자군과 의식 소실이 없었던 환자군으로 구분하여 두 집단의 외상후 스트레스 장애의 유병율, 증상 및 불안 정도를 비교해보고자 하였다. 방 법 : 사고를 당하고 1달 이상 경과한 환자 120명(남자 69명, 여자 51명)을 대상으로 하였다. 대상군을 의식 소실 유무에 따라 소실군과 비소실군으로 구분하고 사회인구학적 변수, 사고 당시 의식 소실의 유무, 의식 소실 시간, 사고의 종류, 사고에 대한 기억, 사고의 심각도, 손상 심각도 점수(Injury Severity Score)를 조사하여비교하였다. 또한 외상후 스트레스 장애 척도(Clinician Adminstered PTSD Scale), Beck의 우울증 척도(Beck Depression Inventory), 상태 특성 불안처도- Ⅰ, Ⅱ(State Trait Anxiety Inventory- Ⅰ, Ⅱ)를 이용하여 조사하여 두 군 사이의 외상후 스트레스 장애유병율 및 증상, 우울, 불안 정도를 비교하였다. 결 과 : 통 연구 대상자 120명중 의식 소실이 있었던 환자군(소실군)은 56명(46.7%)이었고 의식 소실이 없었던 환자군(비소실군)은 64명(53.3%)이었다. 총 대상자 120명중 30명(25%)이 외상후 스트레스 장애로 진단되었으며 소실군 56명중 17명(30.4%)이 비소실군 64명중 13명(20.3%0이 각각 외상후 스트레스 장애로 진단되었으나 두 군간의 차이는 통계적으로 유의하지 않았다(x^2=1.607, P>0.05). 외상후 스트레스 장애 증상 척도에서는 소실군에서 사고에 대한 기억 상실, 흥미 상실, 이탈, 감정 둔마, 수면자애, 예민함, 집중력 감소 증상의 빈도가 유의하게 높았다(P<0.05). 비소실군에서느 반복적인 회상과 놀람 반응 빈도가 유의하게 높았다(p<0.05). Beck의 우울증 척도 점수는 소실군에서 38.8±8.8, 비소실군에서 31.0±7.9로 의식 소실을 보고한 환자군의 점수가 유의하게 높았으며(t=-3.16, 0<0.05) 상태 특성 불안 척도-I, II는 소실군에서 각각 44.5±3.3, 44.6±3.9, 비소실군에서 각각 42.9±3.0, 42.3±3.4로 소실군의 점수가 유의하게 높았다(t=-2.75, p<0.05, t=-3.38, p<0.05). 결 론 : 사고 당시 의식 소실이 없었던 환자군과 비교하여 외상후 스트레스장애가 비슷한 빈도로 진단되며 의식 소실이 외상후 스트레스 장애의 발생에 유의한 영향을 미침은 물론 불안 및 우울에도 유의한 영향을 미친다고 할 수 있다. Objectives :This study investigated PTSD prevalence, symptoms, depressed mood and anxiety, comparing two groups of the patients in trauma ; one who had experienced the loss of consciousness(group "A" here after) and the other group of patients who had not(group "B" here after). Method : subjects were 120 patients(age18-66) who had received trauma(traffic accident, fall down) more than 1 month. Before they consisted of 56 patients who had experienced the loss of the consciousness(46.7%) and 64 who had not(53.3%). Men were 69 and women were 51. Clinician administered PTSD Scale, Beck Depression Inventory, State Trait Anxiety Inventory-I, II were administered. The factors such as sociodemographic variables, unconsciousness at the time of trauma, memory about tranmatic accidents were considered. Result : Out of 120 subjects, 30 patients(25%) were diagnosed as PTSD : 17 from 56(30.4%) and 13 from 64(20.3%) were found in the group "A" and "B" respectively. Prevalence of PTSD was higher in the group "A" showed significant difference between two groups(x^2=1.607,p>0.05). The CAPS of group "A" showed significantly high frequency in the loss of interest, detachment, restricted affect, sleep difficulty, irritability/anger. concentration difficulty(p<0.05), while the group "B" scored high in intrusive recall and increased startle. The scores of BDI, STAI-I, II were significantly higher in the group "A"(t=-3.16, p<0.05)(t=-2.75, p<0.05, t=-3.38, p<0.05). Conclusion : PTSD was mre frequent and depressed mood and anxiety appeared more often in the group qho experienced the loss of consciousness. Thus the loss of the consciousness at the time of trauma prones of the aevelopment PTSD and symptom of aepression and anxiety.

열원이 존재하는 작업장내 기류 및 온도장 예측

정유진,하현철,김태형,유근종 한국산업위생학회 2001 한국산업보건학회지 Vol.11 No.1

A CFD simulation of airflow and temperature filed in a heated room has been described in this paper. The thermal wall jet created by a radiator greatly influences the airflow pattern, temperature distribution. The area close to a heat source has a high risk of air-borne contamination and imposes a harmful effect on occupants in that area. The predicted flow field, temperature results show good agreement with the measured data. As the results were compared with experimental data. the applicability of CFD was satisfactorily verified. Also, the CFD simulation can capture the natural convective flow features. If a CFD simulation is applied ventilation design with a heat source, An effective design will be attained. Further study is required to improve the accuracy of CFD simulation.

화상환자에서 혈청중 가용성 Interleukin-2 Receptor의 변동

이희성,이유진,이종량,백광진,하재성,전건수 大韓成形外科學會 1991 Archives of Plastic Surgery Vol.18 No.2

Cell-mediated immunity frequently becomes severely impaired after burn. However, the cause of postburn immune dysfunction is unclear and controversy exists over both pathophysiology and clinical relevance of these abnormalities. This study was undertaken to invstigate the immune responses in vivo of patients with burn. Levels of soluble IL-2R, a sensitive marker of T-cell activation, were measured in 26 patients with burn. Serum soluble IL-2R levels in patients with burn were significantly higher than those of normal controls. Serum soluble IL-2R levels were correlated closely with the severity of the burn. The burn patients were classified according to the outcome. Patients with fatal outcom always showd a higher soluble IL-2R levels than survivors. Postburn sera significantly suppressed mitogen-indeced lymphocyte proliferation. These data indicate that soluble IL-2R might be useful parameters for monitoring of the clinical course in burn patients.

Mycophenolic Acid와 Rapamycin이 흰쥐 사구체 혈관간세포증식과 세포외기질 생성에 미치는 영향

김명수,박제현,하헌주,허규하,서지연,김유선,김혜진,박기일 이화여자대학교 약학연구소 2004 藥學硏究論文集 Vol.- No.14

Background: Excess proliferation and extracellular matrix (ECM) accumulation of mesenchymal cells such as vascular smooth muscle cells (VSMC) and glomerular mesangial cells cause chronic allograft nephropathy showing transplant vascular sclerosis and glomerulosclerosis. Mycophenolic acid (MPA) and rapamycin (RPM) are well known as strong inhibitors of VSMC proliferation, but their effects on the glomerular mesangial cells are not yet clearly understood. This study examined the effects of MPA or RPM on PDGF-induced proliferation and ECM accumulation in rat glomerular mesangial cells. Methods: Mesangial cells isolated from the glomeruli of Sprague-Dawley rats were cultured with DMEM containing 20% fetal bovine serum. Growth arrested and synchronized cells were administered with test drugs (MPA10 nM-10μM, RPM 0.1 nM-1μM) before the addition of PDGF 10 ng/mL. Cell proliferation was assessed by [³H]thymidine incorporation, collagen by [³H]proline incorporation, and fibronectin, ERK, and p38 MAPK by Western blot analysis. Results: PDGF increased mesangial cell proliferation by 4.64-fold. Compared to stimulated control, MPA above 500 nM and RPM above 10nM showed a significant inhibitory effect in a dose-dependent manner. The IC_(50) of MPA and RPM against PDGF-induced mesangial cell proliferation were around 500 nM and 100 nM, respectively. The collagen synthesis was also inhibited by MPA and RPM, but the fibronectin secretion was inhibited by MPA alone. The proliferation of mesangial cell correlated with activation of ERK and. p38 MAPK. MPA, but not RPM, inhibited ERK and p38 MAPK activation. Conclusion: This study demonstrated that MPA and RPM significantly inhibited PDGF-induced proliferation and ECM production in rat glomerular mesangial cells. The inhibitory effects of MPA, but not RPM, are correlated with ERK and p38 MAPK.

Proliferation Effect of Conditioned Medium Produced by Lymph Node Stromal Cells

Lee, Sang-Han,Moon, Sung-Hoon,Ha, Sang-Chul,Ma, Jin-Yeul,Kim, Min-Sook,Lee, Jin-Man,Kwon, O-Yu Korean Society of Life Science 2002 Journal of Life Science Vol.12 No.1

CS21 lymphoma cells that preferentially metastasize to lymph nodes after s.c. inoculation into BALB/c mice were grown in vitro in the presence of CA12 stromal cells isolated from lymph nodes. To obtain fundamental data on identification and characterization of the soluble factor(s) produced by CA12 stromal cells, we investigated the biological profile of the conditioned medium produced by CAl2 stromal cells. CAl2 conditioned medium has no affinity with Con A. CAl2 conditioned medium is associated with the proliferation of splenic T- and thymic T-cells with-out adding mitogen, although the conditioned medium cannot induce the differentiation of thymocytes. Additionally, we showed that H-7, not HA-1004 inhibits CS21 cell proliferation. These results suggest that CAl2 conditioned medium has a specific soluble factor(s) produced by lymph node stromal cells.