Anti-inflammatory and Anti-oxidant Effects of PHBV/Collagen (PHCP) on Lipopolysaccharide-Induced Skin Inflammation

Jin-Yi Han3*, Xu Zi Guang, Jyung-Sik Kwak, Ki-Wan Oh, Han-Ik Bae 충북대학교 동물의학연구소 2012 Journal of Biomedical and Translational Research Vol.13 No.1

The anti-inflammatory effect of PHBV/Collagen (PHCP) was examined in a mouse model of lipopolysaccharide (LPS)-induced skin inflammation. Vascular permeability on the back skin was measured by the local accumulation of Evan’s blue dye after subcutaneous injection of LPS (30 μg site-1). Dye leakage in the skin showed a significant increase at 2 h after injection of LPS. This LPS-induced dye leakage was also completely inhibited by HO-1 inhibitor, ZnPP, and antioxidants, including methyl gallate, trolox, and mannitol. To study the possible mechanisms underlying the in vivo anti-inflammatory effect of PHCP against LPS-induced inflammation, we also examined the effects of PHCP on malondialdehyde (MDA) and glutathione levels in skin tissues and found that pretreatment with PHCP resulted in inhibited MDA elevation and a remarkable reduction of glutathione level. In addition, similar results were obtained after pretreatment with antioxidants, including trolox and mannitol, and HO-1 inhibitor, ZnPP. Histopathologically, an influx of neutrophils into the skin dermis was detected between 24 h and 72 h after LPS injection (30, 100 μg site-1), compared to control animals after injection of saline. This increase was greater in mice treated with 100 μg of LPS than in those treated with 30 μg of LPS and was significantly suppressed by pretreatment with PHCP, antioxidants, and HO-1 inhibitor. These results collectively suggest that PHCP has an anti-inflammatory effect against LPS-induced inflammation model in vivo and may be a good candidate for the skin tissue engineering biomedical application primarily through manipulation of the redox state.

單症性 健康念慮症의 臨床經驗

韓辰熙,柳太烈,趙伯紀,李鍾六 서울醫大 1990 精神醫學 Vol.15 No.2

Four new compounds from the bulbs of Lycoris aurea with neuroprotective effects against CoCl2 and H2O2-induced SH-SY5Y cell injuries

An Jin,Xuelian Xiang,Yun-Yun Zhu,Heng-Yi Yu,Hui-Fang Pi,Peng Zhang,Han-Li Ruan 대한약학회 2014 Archives of Pharmacal Research Vol.37 No.3

Three new alkaloids, 2a-hydroxy-6-O-n-butyloduline,O-n-butyllycorenine, (-)-N-(chloromethyl)lycoramine(1–3), and a new phenolic compound, ((7S)-7-(4-hydroxyphenyl)-7-hydroxypropyl)-20-methylbenzene-30,60-diol (14), along with ten known alkaloids (4–13), wereisolated from the bulbs of Lycoris aurea collected fromHuaihua County of Hunan Province, China. Their structureswere elucidated by spectroscopic methods includingHRESIMS, UV, IR, and NMR. All the isolated compoundswere tested for their neuroprotective effects against CoCl2and H2O2-induced SH-SY5Y cell death. Compounds 1–7and 10 exhibited significant neuroprotective effects againstCoCl2-induced SH-SY5Y cell injury, while compounds1–5, 7, 10 and 12 showed obvious neuroprotective effectsagainst H2O2-induced SH-SY5Y cell death.

보행속력과 동작의 부드러움과의 상관관계에 관한 연구

탁계래,한영민,최진승,이정한,임영태,전재훈,박상균,박승하,Stefanyshin Darren 한국운동역학회 2006 한국운동역학회지 Vol.16 No.1

저크비용함수를 이용한 골프 숙련자와 초보자간의 퍼팅 동작 분석

임영태,최진승,한영민,김형식,이정한,전재훈,탁계래 한국운동역학회 2006 한국운동역학회지 Vol.16 No.1

『남양선생시집』 간행과 『고려대장경』 조성사업

최연주 ( Choi Yeon-joo ),전진이 ( Jun Jin-yi ),한홍익 ( Han Hong-ik ) 한국밀교학회 2022 불교학밀교학연구 Vol.1 No.-

『남양선생시집』은 고려 무인집권기에 문신관료였던 백분화의 시집이다. 그는 스스로 參禪居士라 할 정도로 禪法을 좋아하였다. 이 시집은 백분화의 장남인 희심이 간행을 주도하였다. 승려이자 문인이었던 희심은 부친이 생전에 남긴 시 2백여 首를 수습해 上下卷으로 편찬하고, 제목과 서문을 이규보 아들인 이함에게 의뢰하였다. 백분화는 이규보보다 나이가 10살 적었지만 일찍 出仕하였다. 최씨정권과 밀착되었던 백분화는 이규보의 출사를 위해 다방면으로 도움을 주었을 것이다. 이는 두 사람이 주고 받은 詩와 이규보가 지은 백분화의 묘지명을 통해 충분히 짐작할 수 있다. 이러한 두 사람의 관계는 아들인 희심과 이함에게 자연스럽게 이어져 시집의 제목과 서문 작성 배경이 되었을 것이다. 『남양선생시집』은 『고려대장경』 조성사업이 마무리된 직후인 고종 36년(1249)에 간행되었다. 시집에서 惠堅과 孝眞이라는 각수가 조사되었는데, 이들은 『고려대장경』 판각에 참여한 각수이다. 시집 간행이 『고려대장경』 판각과 밀접하게 연관되어 있음을 보여준다. 『남양선생시집』 간행 과정을 검토해 보면 이규보의 『동국이상국집』 간행과 유사한 점이 많다. 그렇다면 『고려대장경』 조성사업의 체계를 활용하여 분사대장도감(이하 분사도감으로 약칭함)에서 『남양선생시집』을 간행하였을 개연성이 매우 높다. 『Namyang Seonsaeng Sijib』(『南陽先生詩集』) is a collection of Beak Bunhwa(白賁華) in a period of the Choi military regime. He had continuously interchanged with the Buddhist community including reputed monks. 『Namyang Seonsaeng Sijib』 was published by monk Huisim(希諗), the eldest son of Beak Bunhwa. Twenty-five years after the death of his father, Huisim had collected scattered poems and compiled them into two volumes. Yi Ham(李涵), the son of Yi Gyubo(李奎報), wrote the preface of it. Likewise, Yi Gyubo wrote the epitaph of Beak Bunhwa. Beak Bunhwa and Yi Gyubo were deeply involved in politics and literature. To sum up this causality, these two families had a quite friendly relationship. Meanwhile, 『Namyang Seonsaeng Sijib』 was released immediately after the engraving of 『Tripitaka Koreana』. Hyegyeon(惠堅) and Hyojin(孝眞), engravers who participated in the engraving 『Tripitaka Koreana』, undertook repeatedly the editing of 『Namyang Seonsaeng Sijib』. It shows that publication of 『Namyang Seonsaeng Sijib』 and engraving of 『Tripitaka Koreana』 are closely linked. Beak Bunhwa and Yi Gyubo are politicians who cooperated with the Choi military regime. As 『Donggukyisanggukjip』(『東國李相國集』), the collection of Yi Gyubo, was produced in Bunsadogam(分司都監) with the support of King Gojong and the Choi military regime, it is highly likely that 『Namyang Seonsaeng Sijib』 was produced in the same time.

Efficient Generation of Dopaminergic Neurons from Mouse Ventral Midbrain Astrocytes

Han Jin Yi,Lee Eun-Hye,Kim Sang-Mi,Park Chang-Hwan 한국응용약물학회 2023 Biomolecules & Therapeutics(구 응용약물학회지) Vol.31 No.3

Parkinson’s disease (PD) is a common neurodegenerative disorder characterized by tremors, bradykinesia, and rigidity. PD is caused by loss of dopaminergic (DA) neurons in the midbrain substantia nigra (SN) and therefore, replenishment of DA neurons via stem cell-based therapy is a potential treatment option. Astrocytes are the most abundant non-neuronal cells in the central nervous system and are promising candidates for reprogramming into neuronal cells because they share a common origin with neurons. The ability of neural progenitor cells (NPCs) to proliferate and differentiate may overcome the limitations of the reduced viability and function of transplanted cells after cell replacement therapy. Achaete-scute complex homolog-like 1 (Ascl1) is a wellknown neuronal-specific factor that induces various cell types such as human and mouse astrocytes and fibroblasts to differentiate into neurons. Nurr1 is involved in the differentiation and maintenance of DA neurons, and decreased Nurr1 expression is known to be a major risk factor for PD. Previous studies have shown that direct conversion of astrocytes into DA neurons and NPCs can be induced by overexpression of Ascl1 and Nurr1 and additional transcription factors genes such as superoxide dismutase 1 and SRY-box 2. Here, we demonstrate that astrocytes isolated from the ventral midbrain, the origin of SN DA neurons, can be effectively converted into DA neurons and NPCs with enhanced viability. In addition, when these NPCs are inducted to differentiate, they exhibit key characteristics of DA neurons. Thus, direct conversion of midbrain astrocytes is a possible cell therapy strategy to treat neurodegenerative diseases.

Flavonoids and Their Free Radical Reactions

Jin-Yi Han, Jin-Tae Hong, Sang-Yoon Nam, Ki-Wan Oh 충북대학교 동물의학연구소 2009 Journal of Biomedical and Translational Research Vol.10 No.2

본문참고

Alleviation of Kainic Acid-Induced Brain Barrier Dysfunction by 4-O-Methylhonokiol in <i>In Vitro</i> and <i>In Vivo</i> Models

Han, Jin-Yi,Ahn, Sun-Young,Yoo, Jae Hyeon,Nam, Sang-Yoon,Hong, Jin Tae,Oh, Ki-Wan Hindawi Publishing Corporation 2015 BioMed research international Vol.2015 No.-

<P>This experiment was designed to investigate whether 4-O-methylhonokiol (MH), a principal ingredient of <I>Magnolia (M.) officinalis</I> bark, alleviated acute intraperitoneal (i.p.) kainic acid- (KA-) induced brain blood barrier dysfunction (BBBD) <I>via</I> pathological examination and cytological analyses of the brain tissues of mice. KA (10–30 mg/kg) time- and dose-dependently increased the water content of brain tissues and induced edema and encephalopathy. However, pretreatment with MH (5 and 20 mg/kg, i.p.) significantly reduced the water content of the brain compared to that observed in the KA control group. Furthermore, MH significantly and dose-dependently reversed the remarkable variations in evan's blue dye (EBD) staining and malondialdehyde (MDA) levels that were induced by KA (10 mg/kg, i.p.). MH also decreased the elevated seizure scores that were induced by KA (10 mg/kg, i.p.) in mice in a manner similar to scavengers such as DMTU and trolox. Additionally, MH significantly scavenged intracellular ROS and Ca<SUP>2+</SUP> within hippocampal cells. The tight junction seals mediated by claudin (Cld-5) were also found to be modulated by MH. MH efficiently reduced 1,1-diphenyl-2-picrylhydrazyl (DPPH) (IC<SUB>50</SUB>, 52.4 mM) and <SUP>•</SUP>OH with an electron spin resonance (ESR) signal rate constant of 4 × 10<SUP>9</SUP> M<SUP>−1</SUP> · S<SUP>−1</SUP>, which is close to the reactivity of the vitamin E analog trolox. Taken together, these results suggest that MH may enhance radical scavenging in lipid and hydrophobic environments, which may be important for the physiological activity of the barrier.</P>

Anti-inflammatory and Anti-oxidant Effects of PHBV/Collagen (PHCP) on Lipopolysaccharide-Induced Skin Inflammation

Jin-Yi Han,Xu Zi Guang,Jyung-Sik Kwak,Ki-Wan Oh,Han-Ik Bae 충북대학교 동물의학연구소 2012 Journal of Biomedical and Translational Research Vol.13 No.1

The anti-inflammatory effect of PHBV/Collagen (PHCP) was examined in a mouse model of lipopolysaccharide (LPS)-induced skin inflammation. Vascular permeability on the back skin was measured by the local accumulation of Evan’s blue dye after subcutaneous injection of LPS (30 µg site^-1 ). Dye leakage in the skin showed a significant increase at 2 h after injection of LPS. This LPS-induced dye leakage was also completely inhibited by HO-1 inhibitor, ZnPP, and antioxidants, including methyl gallate, trolox, and mannitol. To study the possible mechanisms underlying the in vivo anti-inflammatory effect of PHCP against LPS-induced inflammation, we also examined the effects of PHCP on malondialdehyde (MDA) and glutathione levels in skin tissues and found that pretreatment with PHCP resulted in inhibited MDA elevation and a remarkable reduction of glutathione level. In addition, similar results were obtained after pretreatment with antioxidants, including trolox and mannitol, and HO-1 inhibitor, ZnPP. Histopathologically, an influx of neutrophils into the skin dermis was detected between 24 h and 72 h after LPS injection (30, 100 µg site^-1), compared to control animals after injection of saline. This increase was greater in mice treated with 100 µg of LPS than in those treated with 30 µg of LPS and was significantly suppressed by pretreatment with PHCP, antioxidants, and HO-1 inhibitor. These results collectively suggest that PHCP has an anti-inflammatory effect against LPS-induced inflammation model in vivo and may be a good candidate for the skin tissue engineering biomedical application primarily through manipulation of the redox state.