Tetrahydrofuran-Containing Crown Ethers as Ionophores for NH⁺₄-Selective Electrodes

Jin, Hua-Yan,Kim, Tae-Ho,Kim, Jin-Eun,Lee, Shim-Sung,Kim, Jae-Sang Korean Chemical Society 2004 Bulletin of the Korean Chemical Society Vol.25 No.1

The ammonium ion-selective electrodes ($NH^+_4$-ISEs) based on the tetrahydrofuran(THF)-containing-16-crown-4 derivatives,1,4,6,9,11,14,16,19-tetraoxocycloeicosane ($L^1$) and 5,10,15,20,-tetramethyl-1,4,6,9,11,14,16,19-tetraoxocycloeicosane ($L^2$), were prepared and the electrode characteristics were tested. The conditioned $NH_4^+$-ISEs (E1) based on $L^1$ with TEHP as a plasticising solvent mediator gave best results with near-Nernstian slope of 53.9 mV/decade of activity, detection limit of $10^{-4.9}$ M, and enhanced selectivity coefficients for the $NH^+_4$ ion with respect to an interfering $K^+$ ion (log $K^{pot}_{NH_4^+,K^+}$ = -1.84). This result was compared to other ammonium ionophores reported previously, for example, that of nonactin (log $K^{pot}_{NH_4^+,K^+}$ = -0.92). The proposed electrode showed no significant potential changes in the range of 3.0 < pH < 9.0.

Content-based Image Retrieval Using Color Adjacency and Gradient

Jin, Hong-Yan,Lee, Ho-Young,Kim, Hee-Soo,Kim, Gi-Seok,Ha, Yeong-Ho The Institute of Electronics and Information Engin 2001 電子工學會論文誌-SP (Signal processing) Vol.38 No.1

본 논문에서는 칼라 인접성과 기울기를 이용한 새로운 내용 기반 영상 검색 방법을 제안한다. 칼라 영상의 특징 정보로 사용되는 칼라 히스토그램은 시점이나 영상의 회전등의 영향을 적게 받고 특징 정보의 계산이 간단하고 빠른 장점이 있지만 칼라의 위치 정보를 나타낼 수 없기 때문에 균일 양자화에 의해 비슷한 히스토그램을 가진 서로 다른 영상을 구별하지 못하고 특징 저장량이 많은 등 단점이 있다. 제안한 방법은 기존의 방법들에서 보편적으로 사용하는 양자화 대신 영상에서의 인접 화소의 칼라 변화량 즉 기울기를 계산하여 보다 정확한 색차를 구함으로써 비슷한 칼라가 서로 다르게 양자화됨으로 인한 오차를 감소시켰다. 동시에 영상의 주요 칼라 구성 특징을 나타나는 칼라 인접성 정보를 추출하여 이진 배열로 표시함으로써 특징 정보의 방대한 저장량을 줄이고 비교속도를 향상시켰다. 실험 결과 기존의 검색 방법에 비하여 제안한 방법은 적은 특징 저장 양으로 외부조건의 변화에 더욱 강건함을 보여주고 있다. A new content-based color image retrieval method integrating the features of the color adjacency and the gradient is proposed in this paper. As the most used feature of color image, color histogram has its own advantages that it is invariant to the changes in viewpoint and the rotation of the image etc., and the computation of the feature is simple and fast. However, it is difficult to distinguish those different images having similar color distributions using histogram-based image retrieval, because the color histogram is generated on uniformly quantized colors and the histogram itself contains no spatial information. And another shortcoming of the histogram-based image retrieval is the storage of the features is usually very large. In order to prevent the above drawbacks, the gradient that is the largest color difference of neighboring pixels is calculated in the proposed method instead of the uniform quantization which is commonly used at most histogram-based methods. And the color adjacency information which indicates major color composition feature of an image is extracted and represented as a binary form to reduce the amount of feature storage. The two features are integrated to allow the retrieval more robust to the changes of various external conditions.

The TO901317, an agonist of LXRα, Increased Mesangial Matrix and Renal Collagen 4 with the Enhancement of SREBP1 in Diabetic Animals

푸얀얀 ( Yan Yan Fu ),이은성 ( En Sung Lee ),이윤미 ( Yun Mi Lee ),유선진 ( Sun Jin You ),송영림 ( Young Rim Song ),진호준 ( Ho Jun Chin ),나기영 ( Ki Young Na ),김성권 ( Suhng Gwon Kim ),채동완 ( Dong Wan Chae ) 대한신장학회 2008 춘계학술대회 초록집 Vol.28 No.1

칼라 인접성과 기울기를 이용한 내용 기반 영상 검색

김홍염(Hong-Yan Jin),이호영(Ho-Young Lee),김희수(Hee-Soo Kim),하영호(Yeong-Ho Ha) 대한전자공학회 2000 대한전자공학회 학술대회 Vol.2000 No.6

Predictive test for chemotherapy response in resectable gastric cancer: a multi-cohort, retrospective analysis

Cheong, Jae-Ho,Yang, Han-Kwang,Kim, Hyunki,Kim, Woo Ho,Kim, Young-Woo,Kook, Myeong-Cherl,Park, Young-Kyu,Kim, Hyung-Ho,Lee, Hye Seung,Lee, Kyung Hee,Gu, Mi Jin,Kim, Ha Yan,Lee, Jinae,Choi, Seung Ho,Ho Elsevier 2018 LANCET ONCOLOGY Vol.19 No.5

<P><B>Summary</B></P> <P><B>Background</B></P> <P>Adjuvant chemotherapy after surgery improves survival of patients with stage II–III, resectable gastric cancer. However, the overall survival benefit observed after adjuvant chemotherapy is moderate, suggesting that not all patients with resectable gastric cancer treated with adjuvant chemotherapy benefit from it. We aimed to develop and validate a predictive test for adjuvant chemotherapy response in patients with resectable, stage II–III gastric cancer.</P> <P><B>Methods</B></P> <P>In this multi-cohort, retrospective study, we developed through a multi-step strategy a predictive test consisting of two rule-based classifier algorithms with predictive value for adjuvant chemotherapy response and prognosis. Exploratory bioinformatics analyses identified biologically relevant candidate genes in gastric cancer transcriptome datasets. In the discovery analysis, a four-gene, real-time RT-PCR assay was developed and analytically validated in formalin-fixed, paraffin-embedded (FFPE) tumour tissues from an internal cohort of 307 patients with stage II–III gastric cancer treated at the Yonsei Cancer Center with D2 gastrectomy plus adjuvant fluorouracil-based chemotherapy (n=193) or surgery alone (n=114). The same internal cohort was used to evaluate the prognostic and chemotherapy response predictive value of the single patient classifier genes using associations with 5-year overall survival. The results were validated with a subset (n=625) of FFPE tumour samples from an independent cohort of patients treated in the CLASSIC trial (NCT00411229), who received D2 gastrectomy plus capecitabine and oxaliplatin chemotherapy (n=323) or surgery alone (n=302). The primary endpoint was 5-year overall survival.</P> <P><B>Findings</B></P> <P>We identified four classifier genes related to relevant gastric cancer features (<I>GZMB, WARS, SFRP4</I>, and <I>CDX1</I>) that formed the single patient classifier assay. In the validation cohort, the prognostic single patient classifier (based on the expression of <I>GZMB, WARS</I>, and <I>SFRP4</I>) identified 79 (13%) of 625 patients as low risk, 296 (47%) as intermediate risk, and 250 (40%) as high risk, and 5-year overall survival for these groups was 83·2% (95% CI 75·2–92·0), 74·8% (69·9–80·1), and 66·0% (60·1–72·4), respectively (p=0·012). The predictive single patient classifier (based on the expression of <I>GZMB, WARS</I>, and <I>CDX1</I>) assigned 281 (45%) of 625 patients in the validation cohort to the chemotherapy-benefit group and 344 (55%) to the no-benefit group. In the predicted chemotherapy-benefit group, 5-year overall survival was significantly improved in those patients who had received adjuvant chemotherapy after surgery compared with those who received surgery only (80% [95% CI 73·5–87·1] <I>vs</I> 64·5% [56·8–73·3]; univariate hazard ratio 0·47 [95% CI 0·30–0·75], p=0·0015), whereas no such improvement in 5-year overall survival was observed in the no-benefit group (72·9% [66·5–79·9] in patients who received chemotherapy plus surgery <I>vs</I> 72·5% [65·8–79·9] in patients who only had surgery; 0·93 [0·62–1·38], p=0·71). The predictive single patient classifier groups (chemotherapy benefit <I>vs</I> no-benefit) could predict adjuvant chemotherapy benefit in terms of 5-year overall survival in the validation cohort (p<SUB>interaction</SUB>=0·036 in univariate analysis). Similar results were obtained in the internal evaluation cohort.</P> <P><B>Interpretation</B></P> <P>The single patient classifiers validated in this study provide clinically important prognostic information independent of standard risk-stratification methods and predicted chemotherapy

Life Science : original article ; Discovery, design and synthesis of Y-shaped peroxisome proliferator-activated receptor agonists as potent anti-obesity agenys in vivo

( Jung Yeob Ham ),( Hoo Sang Hwang ),( Euno Kim ),( Jeong Ah Kim ),( Sung Jin Cho ),( Jae Young Ko ),( Woo Jin Lee ),( Jae Hwan Lee ),( Harish Holla ),( Joydeep Banerjee ),( Seok Ho Kim ),( Jin Ho Yan 영남대학교 약품개발연구소 2012 영남대학교 약품개발연구소 연구업적집 Vol.22 No.0

We have discovered and demonstrated the in vitro and in vivo PPARδ-selective activity of novel Y-shaped agonists. These compounds activated hPPARδ with EC(50) values between 1 and 523 nM. Surprisingly, compounds 10a, 11d, 11e and 11f were the most potent and most selective hPPARδ agonists with 10(4)-fold selectivity over the other two subtypes, namely, hPPARα and hPPARγ. The PPARδ ligands 10a, 11e and 11f showed good bioavailability and in vivo efficacy. ⓒ 2012 Elsevier Masson SAS. All rights reserved.

林蛙(Rana temporaria chinesis David) 皮膚 色素細胞의 徵細構造

金龍勳,文仁祜,嚴昌國,盧鏞泰,朴仁峰 建國大學校基礎科學硏究所 1992 理學論集 Vol.17 No.-

林蛙의 皮膚色素胞인 멜라닌色素胞,黃色素胞 및 紅色素胞를 電子顯微鏡으로 觀察하여 다음과 같은 結果를 얻었다. 멜라닌色素胞 : 멜라닌色素胞의 全 細胞質에는 電子密度가 같은 멜라닌 色素顆粒으로 충만되어 있으며, 核은 細胞質 한쪽으로 치우쳤고 楕圓形이거나 不定形이다. 대부분의 멜라닌色素胞는 細胞質突起를 가지며 黃色素胞와 紅色素胞 사이에 나타나는 경우가 많았다. 黃色素胞 : 黃色素胞는 다수의 pterinosome과 소수의 carotenoid vesicle의 色素小器官으로 構成되었다. 그중 pterinosomem 은 小囊 속의 內容物質에 따라, 內容物質이 없는 것을 第1型 pterinosome, 小囊 속에 纖維質이 散在한 것을 第 2 型 pterinosome. 小囊 속에 소수의 lamel-lae層을 形成한 것을 第 3 型 pterinosome,그리고 小囊 속에 다수의 lamellae를 形成한 것을 第 4 型 pterinosome으로 區別.觀察하였다. 紅色素胞 : 紅色素胞는 長方形 等 不定形의 反射小板으로 채워져 있다. 黃色素胞와 멜라닌色素胞,멜라닌色素胞와 멜라닌色素胞 사이 等에 連接하였다. The ultrastructures of the pigment cells in the Rana temporaria chinensis dorsal skin were observed with electron microscope. The results of the fine structure in the melanophores,xanthophores and iridophores were as follow: Melanophores:Epidermal melanophores were filled with melanin granules which ap-peared as the same electron density. A few melanin granules were observed in a cornified cell. Dermal melanophores contained numerous melanin granules extended up the lateral sides of the xanthophores and iridophores. Xanthophores:Xanthophores were filled with pterinosomes and carotenoid vesicles. Type I pterinosomes had a clear limiting membrane. Type Ⅱ pterinosomes had the inner fibrous structure. Type Ⅲ pterinosomes were charavterized by a few superficial lamellae and Type Ⅳ pterinosomes by multiple concentric lamellae. Iridophores:Iridophores were filled with reflective platelets,each of which is rectangle and shapelessness.

Increased cyclooxygenase-2 and nuclear factor-κB/p65 expression in mouse hippocampi after systemic administration of tetanus toxin

YAN, BING CHUN,JEON, YONG HWAN,PARK, JOON HA,KIM, IN HYE,CHO, JEONG-HWI,AHN, JI HYEON,CHEN, BAI HUI,TAE, HYUN-JIN,LEE, JAE-CHUL,AHN, JI YUN,KIM, DONG WON,CHO, JUN HWI,WON, MOO-HO,HONG, SEONGKWEON SPANDIDOS PUBLICATIONS 2015 MOLECULAR MEDICINE REPORTS Vol.12 No.6

<P>Brain inflammation has a crucial role in various diseases of the central nervous system. The hippocampus in the mammalian brain exerts an important memory function, which is sensitive to various insults, including inflammation induced by exo/endotoxin stimuli. Tetanus toxin (TeT) is an exotoxin with the capacity for neuronal binding and internalization. The present study investigated changes in inflammatory mediators in the mouse hippocampus proper (CA1-3 regions) and dentate gyrus (DG) after TeT treatment. The experimental mice were intraperitoneally injected with TeT at a low dosage (100 ng/kg), while the control mice were injected with the same volume of saline. At 6, 12 and 24 h after TeT treatment, changes in the hippocampal levels of inflammatory mediators cyclooxygenase-2 (COX-2) and nuclear factor kappa-B (NF-κB/p65) were assessed using immunohistochemical and western blot analysis. In the control group, moderate COX-2 immunoreactivity was observed in the stratum pyramidal (SP) of the CA2-3 region, while almost no expression was identified in the CA1 region and the DG. COX-2 immunoreactivity was increased by TeT in the SP and granule cell layer (GCL) of the DG in a time-dependent manner. At 24 h post-treatment, COX-2 immunoreactivity in the SP of the CA1 region and in the GCL of the DG was high, and COX-2 immunoreactivity in the SP of the CA2/3 region was highest. Furthermore, the present study observed that NF-κB/p65 immunoreactivity was obviously increased in the SP and GCL at 6, 12 and 24 h after TeT treatment. In conclusion, the present study demonstrated that systemic treatment with TeT significantly increased the expression of COX-2 and NF-κB/p65 in the mouse hippo-campus, suggesting that increased COX-2 and NF-κB/65 expression may be associated with inflammation in the brain induced by exotoxins.</P>

Study on the Effectiveness of High-Speed Railway Communication and Signaling System Based on 4G LTE Technology

Yan Sun,Chang-Young Lee,Jeong-min Jo,Jin-ho Lee,Young-Jae Han 제어로봇시스템학회 2013 제어로봇시스템학회 국제학술대회 논문집 Vol.2013 No.10

Due to speed acceleration, higher requirements have been put forward to high speed railway communication and signaling system. The railway applications, including intra-train, train to ground and trackside networks, demand larger bandwidth, higher reliability and shorter response time from railway communication networks to ensure security operation and passenger communication. However, the current railway communication and signaling system has lagged behind the railway development, so advanced communication technologies are needed to improve the current situation. This paper presents high speed railway communication and signaling system based on 4G LTE technology, introduces its network architecture, key technology and analyzes its technical advantages compared with GSM-R system. According to analysis, LTE based high-speed railway communication network is more effective and reliable.

Serodiagnosis of Toxocariasis by ELISA Using Crude Antigen of Toxocara canis Larvae

Yan Jin,Chenghua Shen,Sun Huh,Woon-Mok Sohn,Min-Ho Choi,Sung-Tae Hong 대한기생충학열대의학회 2013 The Korean Journal of Parasitology Vol.51 No.4