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RISS 인기검색어
- 검색키워드
- 저자 : ( Jin Ah Ryuk ) (검색결과 33 건)
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Ryuk, Jin Ah,Lee, Hye Won,Ju, Young Seong,Ko, Byoung Seob The Korean Society for Applied Biological Chemistr 2014 Applied Biological Chemistry (Appl Biol Chem) Vol.57 No.2
Cynanchum wilfordii (Asclepiadaceae) is widely distributed throughout Korea, Japan, and China. Dried roots of this plant have been used as a tonic to promote renal function. Due to the morphological similarities of the dried roots of this plant to those of Cynanchum auriculatum, which is used as a substitute herbal medicine for C. wilfordii, distinguishing these two species is extremely difficult. The present study was conducted to develop molecular markers to distinguish C. wilfordii and C. auriculatum by using conventional polymerase chain reaction (PCR) and realtime PCR analyses. Comparative analysis based on the sequence of the trnL-trnF intergenic spacer revealed 4 base-pair variations, and the inter-individual sequences of the 2 species separately showed 100% homology. According to these results, the variations were divided into 2 groups. The 2 species were further distinguished using a sequence-characterized amplified region marker developed based on a randomly amplified polymorphic DNA-PCR product, and then a single nucleotide polymorphism marker was designed based on the trnL-trnF intergenic spacer for more efficient detection in real-time PCR. The results showed that species-specific molecular markers might allow accurate discrimination of C. wilfordii and C. auriculatum.
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Discrimination of Lonicera japonica and Lonicera confusa using chemical analysis and genetic marker
Ryuk, Jin Ah,Lee, Hye Won,Ko, Byoung Seob The Korea Association of Herbology 2012 大韓本草學會誌 Vol.27 No.6
Objective : Lonicera japonica THUNB. a traditional herbal medicine, has been commonly used anti-inflammatory disease. It has been very complicated with respect to its sources on the market. The significant selection of medicine depends on its origin. However, it is difficult to discrimination criteria for confirming L. japonica authenticity using the senses. This study was performed to determine the discriminant analysis of L. japonica and L. confusa. Methods : The identification of L. japonica and L. confusa were performed by the classification and identification committee of the national center for standardization of herbal medicines. And we examined its differences using HPLC and genetic marker analysis. Results : The analytical pattern of High Performance Liquid Chromatography was determined from the corresponding peak curves ((E)-aldosecologanin, chlorogenic acid, luteolin 7-O-glucoside, sweroside). For L. japonica, additional unknown peaks were detected at 13.8 min, 20.6 min, and 36.9 min. And, we developed genetic marker using the the tRNA-Leu gene, trnL-trnF intergenic spacer and tRNA-Phe region of chloroplast DNA. By the method, 164 bp PCR product amplified from L. confusa was distinguished into L. japonica and L. confusa efficiently. Conclusion : Base on these results, two techniques provide effective approaches to distinguish L. japonica from L. confusa.
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Ryuk, Jin Ah,Ko, Byoung-Seob,Lee, Hye Won,Kim, Da Sol,Kang, Suna,Lee, Yong Hyen,Park, Sunmin SAGE Publications 2017 Experimental biology and medicine Vol.242 No.6
<P>Tetragonia tetragonioides (Pall.) Kuntze (TTK) and JakYakGamCho-Tang (JGT) have been used for improving women's health and treating inflammatory diseases. We determined that the long-term consumption of these herbal extracts alleviates the progression of postmenopausal symptoms in high-fat-diet fed ovariectomized (OVX) rats, and further explored the mechanisms involved. Five groups of OVX rats were fed high fat diets that were supplemented with either 2% dextrin (control), 2% TTK (70% ethanol extract), 2% JGT (water extract), 1% JGT + 1% TTK (JGTT), or 30 mu g/kg body weight/day of 17 beta-estradiol (positive control). After eight weeks of dietary intervention, the herbal treatments did not change the serum concentrations of 17 beta-estradiol or uterine weight in control rats, but they were higher in the positive-control group. TTK rats exhibited higher daily energy expenditure, particularly fat oxidation, without modifying the energy intake than the controls. TTK lowered the fat mass but lean body mass of the abdomen and leg were increased. JGT decreased periuterine fat mass and lean body mass more than the control but the decrease was not as much as TTK. TTK resulted in substantially lower serum concentrations of the proinflammatory cytokines, tumor necrosis factor-alpha (TNF-alpha) and monocyte chemoattractant protein-1, than the control and JGT had lesser effect than TTK. Insulin resistance, determined by homeostasis model assessment estimate for assessing insulin resistance (HOMA-IR) and insulin tolerance test, was reduced in the decreasing order of control, JGT, JGTT, and TTK and the HOMA-IR of TTK was similar to the positive control. TTK, but not JGT, enhanced glucose tolerance compared with the control, although the serum insulin levels in TTK were lower compared to the control. Interestingly, the beta-cell masses were much greater in the TTK and JGTT groups than in the control, and they were comparable to the positive control. The increases in beta-cell masses in TTK and JGTT groups were associated with enhanced beta-cell proliferation and suppressed apoptosis, which was related to the decreased TNF-alpha and interleukin-1 beta expressions. In conclusion, JGTT did not improve menopausal symptoms better than TTK itself. TTK itself prevented the OVX-induced impairments in energy, lipid, and glucose metabolism, similar to the positive control, without changing serum17 beta-estradiol levels and potentiating insulin signaling and decreasing proinflammatory cytokines. TTK may be a useful intervention to alleviate some menopausal symptoms similar to selective estrogen receptor modulators and should be investigated with further human study.</P>
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Ryuk, Jin Ah,Choi, Go Ya,Kim, Young Hwa,Lee, Hye Won,Lee, Mi Young,Choi, Jae Eul,Ko, Byoung Seob Pharmaceutical Society of Japan 2010 BIOLOGICAL & PHARMACEUTICAL BULLETIN Vol.33 No.7
<P>Forsythiae Fructus has been used as a herbal medicine for a fruit of <I>Forsythia viridissima</I> L<SMALL>INDLEY</SMALL> or <I>Forsythia suspensa</I> V<SMALL>AHL</SMALL> (Oleaceae). In Korea, the fruit of <I>Forsythia viridissima</I> is used and in China, the fruit of <I>Forsythia suspensa</I> is used generally. There are differences in the amount and distribution of constituents between <I>Forsythia viridissima</I> (FV) and <I>Forsythia suspensa</I> (FS). Accordingly, a discrimination of these two herbal drugs is needed. In this study, we designed FV genetic marker based on the internal transcribed spacer (ITS) sequence of nuclear ribosomal DNA that can discriminate <I>Forsythia viridissima</I> and <I>Forsythia suspensa</I> and species-specific amplification product 252 bp was confirmed. Using the real-time polymerase chain reaction (PCR) (allelic discrimination) analysis, an accurate discrimination between <I>Forsythia viridissima</I> and <I>Forsythia suspensa</I> was accomplished. Accordingly, with the use of PCR analysis based on ITS region sequence of ribosomal DNA and the real-time PCR analysis which can efficiently discriminate between <I>Forsythia viridissima</I> and <I>Forsythia suspensa</I> was developed.</P>
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Genetic identification of Sinomenium acutum based on chloroplast gene ndhF sequences
Ryuk, Jin Ah,Lee, Hye Won,Ko, Byoung Seob The Korea Association of Herbology 2013 大韓本草學會誌 Vol.28 No.5
Objectives : This study was conducted to identify the original Sinomini Caulis et Rhizoma plant among Stephania tetrandra, Cocculus trilobus, and Aristolochiae fangchi to develop the genetic marker for Sinomini Caulis et Rhizoma. Methods : Sinomenium acutum was identified by the classification and identification committee of the National Center for Standardization of Herbal Medicines. The chloroplast ndhF gene was amplified. We performed sequences alignment analysis of Sinomenium acutum, Stephania tetrandra, C. trilobus, and A. fangchi using BioEdit program. The SFR markers designed were consisted of SF01, SR04, and SR05 primers. Results : Many variations of Sinomeni Caulis et Rhizoma are currently commercialized as herbal medicine. We compared the base sequences of the ndhF intergenic space of chloroplast DNA with Sinomenium acutum, Stephania tetrandra, C. trilobus, and A. fangchi. According to the results, it showed that the nucleotide variations were seen in 30 genes of four species. Phylogenetic analysis revealed that 4 species were classified into five groups based on an inter-group divergence in nucleotide sequence of 9%. We developed SFR marker nucleotides enough to authenticate respective species and confirmed its application on the band size at 419 base pair. These sequence differences at corresponding positions were available genetic markers to identity the Sinomeni Caulis et Rhizoma. Conclusions : Base on these results, the ndhF region was effective in distinguishing Sinomini Caulis et Rhizoma The SFR genetic marker was useful for identifying Sinomini Caulis et Rhizoma with other species.
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Ryuk, Jin Ah,Zheng, Ming Shan,Lee, Mi Young,Seo, Chang Seob,Li, Ying,Lee, Seung Ho,Moon, Dong Cheul,Lee, Hye Won,Lee, Je-Hyun,Park, Ju Young,Son, Jong Keun,Ko, Byoung Seob 대한약학회 2012 Archives of Pharmacal Research Vol.35 No.6
Phellodendri Cortex is the bark of the stems of Phellodendron amurense Ruprecht or P. chinense Schneider (Rutaceae), which is orginated from periderm. The internal transcribed spacer sequences of 20 originated plants and identified samples were analyzed. The result showed that the 99% of the base sequences of P. amurense were identical to that of P. chinense, but the differentiation of P. amurense and P. chinense was difficult. In addition, the ribulose-1, 5-bisphospate carboxylase large subunit (rbcL) intergenic spacer sequences of specific parts produced the same result. However, when the analysis was carried out by using the RAPD (randomly amplification polymorphism DNA) analysis method, which utilizes 48 randomly primers, it allowed us to confirm the polymorphism of P. amurense and P. chinense in 12 primers. A high-performance liquid chromatographic (HPLC) method was developed and validated for the simultaneous quantitation of berberine, palmatine and jatrorrhizine in a traditional herbal drug, Phellodendri Cortex. The HPLC method was applied successfully to the quantification of three constituents in the extract of twenty Phellodendri Cortex. The results indicated that the established HPLC and RAPD methods are suitable for the quantitative analysis and the quality control multi-simultaneous discrimination in Phellodendri Cortex.
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Ryuk, Jin Ah,Lixia, Mu,Cao, Shihua,Ko, Byoung-Seob,Park, Sunmin Elsevier 2017 COMPLEMENTARY THERAPIES IN MEDICINE Vol.33 No.-
<P><B>Abstract</B></P> <P><B>Objectives</B></P> <P>A systematic review and meta-analysis was conducted to evaluate the efficacy and safety of Gegen Qinlian decoction (GQD) for normalizing hyperglycemia in T2DM patients by pooling all available RCTs.</P> <P><B>Methods</B></P> <P>All relevant RCTs were searched using the keywords: “GQD”, “T2DM”, “hyperglycemia” and “insulin” from the electronic databases including PubMed, EMBASE, Cochrane Library, Korean databases, Chinese medical databases, and Indian scientific database. Each RCT included the control (metformin) and experimental (GQD+metformin) groups. The outcome measures were the assessments of changes in the severity of diabetic symptoms such as “markedly effective” and “effective” (fasting plasma glucose levels: <7 and 7–9, respectively; 2h postprandial glucose levels: <8.3 and 8.3–10.5mmol/L, respectively) after 8 weeks of treatment in each RCT.</P> <P><B>Results</B></P> <P>There were 186 articles selected from the initial searches and 181 irrelevant and duplicate articles were removed. Finally, 5 relevant RCTs involving 499 patients were included in this review. The meta-analysis showed the odds ratio of favorable GQD effect on the marked effectiveness of glycemia (n=499, OR: 2.34, 95% CI: 1.63–3.37, P<0. In a subgroup analysis by GQD composition, 4 RCTs with original GQD composition also showed the odds ratio of the original GQD effect on the marked effectiveness of glycemia (n=339, OR: 2.58; 95% CI=1.65–4.02, P<0.0001) in comparison to the control group. All five studies used an appropriate method for randomization of the subjects but some of them included allocation concealment and blinding of patients and practitioners. There was no significant publication bias in the meta-analysis.</P> <P><B>Conclusion</B></P> <P>The GQD and metformin had a synergistic effect on glycemic control in comparison to metformin alone as a T2DM therapy. More rigorous and larger studies are needed to confirm the therapeutic efficacy of GQD for hyperglycemia due to the moderate to high risk of bias in the 5 RCTs.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Five RCTs including 499 type 2 diabetic patients examined the efficacy of Gegen Qinlian decoction in glycemic control. </LI> <LI> GQD with metformin synergistically enhanced glycemic control in type 2 diabetic patients. </LI> <LI> GQD was not reported any adverse effects. </LI> <LI> More rigorous and larger studies are needed to confirm the therapeutic efficacy of GQD for hyperglycemia. </LI> </UL> </P>
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Discrimination of Lonicera japonica and Lonicera confusa using chemical analysis and genetic marker
Jin Ah Ryuk,Hye Won Lee,Byoung Seob Ko 대한본초학회 2012 大韓本草學會誌 Vol.27 No.6
Objective: Lonicera japonica THUNB. a traditional herbal medicine, has been commonly used anti-inflammatory disease. It has been very complicated with respect to its sources on the market. The significant selection of medicine depends on its origin. However, it is difficult to discrimination criteria for confirming L. japonica authenticity using the senses. This study was performed to determine the discriminant analysis of L. japonica and L. confusa. Methods: The identification of L. japonica and L. confusa were performed by the classification and identification committee of the national center for standardization of herbal medicines. And we examined its differences using HPLC and genetic marker analysis. Results: The analytical pattern of High Performance Liquid Chromatography was determined from the corresponding peak curves ((E)-aldosecologanin, chlorogenic acid, luteolin 7-O-glucoside, sweroside). For L. japonica, additional unknown peaks were detected at 13.8 min, 20.6 min, and 36.9 min. And, we developed genetic marker using the the tRNA-Leu gene, trnL-trnF intergenic spacer and tRNA-Phe region of chloroplast DNA. By the method, 164 bp PCR product amplified from L. confusa was distinguished into L. japonica and L. confusa efficiently. Conclusion: Base on these results, two techniques provide effective approaches to distinguish L. japonica from L. confusa.
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