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( Jia Cheng ),( Na Sun ),( Xin Zhao ),( Li Niu ),( Mei Qin Song ),( Yao Gui Sun ),( Jun Bing Jiang ),( Jian Hua Guo2 ),( Yuan Sheng Bai ),( Jun Ping He ),( Hong Quan Li ) 한국미생물 · 생명공학회 2013 Journal of microbiology and biotechnology Vol.23 No.8
Seventeen compounds derived from traditional Chinese medicines (TCMs) were tested for their antiviral activity against porcine reproductive and respiratory syndrome virus (PRRSV) in vitro. Visualization with the cytopathologic effect (CPE) assay and the 3-(4, 5-dimethyithiazol- 2-yl)-2,5-diphenyltetrazolium bromide test were used to determine the 50% cytotoxic concentration (CC50) and 50% effective concentration (EC50) in cultured Marc-145 cells. Among the tested compounds, chlorogenic acid and scutellarin showed potential anti-PRRSV activity. The EC50 values were 270.8 ± 14.6 μg/ml and 28.21 ± 26.0 μg/ml and the selectivity indexes were >5.54 and 35.5, respectively. The time-of-addition and virucidal assay indicated that the anti-PRRSV activity of the two compounds could be due to their inhibiting the early stage of virus replication and/or inactivating the virus directly. The inhibition of the virus attachment was not observed in the adsorption inhibition assay. The inhibition ratios of chlorogenic acid and scutellarin were, respectively, 90.8% and 61.1% at the maximum non-cytotoxic concentrations. The results have provided a basis for further exploration of their antiviral properties and mechanisms in vivo. We believe that the chlorogenic acid and scutellarin have a great potential to be developed as new anti-PRRSV drugs for clinical application.
Achievement and challenge, the nationality policies of China
Guo Jia-ji 한국사회학회 2003 한국사회학회 기타간행물 Vol.- No.-
Given the circumstances of fifty-six nationalities living together and their culture diversity, Chinese government made nationality policies, such as nationality equality, nationality comity, nationality autonomy and so on. Fully implementing these policies made sure of stability and development of minorities of China over 50 years. However, the system was made under planned economy and implemented by means of central government’s power. So under the circumstances of China’s entry into WTO and market economy, these policies will face huge challenge for going on. How to make a new system of policy which matches market economy and WTO orders is a crucial problem waiting us to resolve.
Guo, Jia,Seong, Eun-Soo,Wang, Myeong-Hyeon The Korean Society for Applied Biological Chemistr 2007 Journal of Applied Biological Chemistry (J. Appl. Vol.50 No.4
We characterized a full-length cDNA of CaCOP1 from pepper. Phylogenetic analysis based on the deduced amino acid sequence of CaCOP1 cDNA revealed high sequence similarity to the COP1 gene in Oryza sativa (84% identity). CaCOP1 shares high sequence identity with regulatory protein in Arabidopsis (84%), constitutively photomorphogenic 1 protein in Pisum sativum (81%) and COP1 homolog in Lycopersicon esculentum (79%). CaCOP1 gene exists single copy in the chili pepper genome. Expression of CaCOP1 was reduced in response to inoculation of non-host pathogens. The expression of this gene under abiotic and oxidative stresses was investigated, including 200 mM NaCl, 200 mM mannitol, cold ($4^{\circ}C$), 100 ${\mu}M$ abscisic acid (ABA), and 10 mM hydrogen peroxide ($H_2O_2$). CaCOP1 was induced significantly 3 h after low temperature treatment but not by dehydration or high salinity. Moreover, CaCOP1 was not induced by plant hormone ABA. These observations suggest that CaCOP1 gene plays a role in abiotic stress and may be belong to ABA-independent regulation system.
The Function of ArgE Gene in Transgenic Rice Plants
Guo, Jia,Seong, Eun-Soo,Cho, Joon-Hyeong,Wang, Myeong-Hyeon The Plant Resources Society of Korea 2007 한국자원식물학회지 Vol.20 No.6
We carried out to study the function of ArgE in transgenic rice plants, which were confirmed by PCR analysis and hygromycin selection. Transgenic rice plants were with selectable marker gene(HPT) inserted in genome of the rice. Southern analysis with hpt probe confirmed by two restriction enzymes that copy numbers of the selectable gene was introduced into the plant genome. We displayed that the relationship between drought stress and ArgE gene with the overexpressing rice plants. From this result, we observed that the degree of leaves damage has no difference in control and transgenic lines. The total RNAs were extracted from 6 weeks-seedling in normal condition in order to examine their expression levels with ArgE-overexpressed transgenic rice. In particular, expression patterns of genes encoding enzymes involved in abiotic stress, including drought and salt stresses. OsGF14a and OsSalt were investigated by reverse transcription-PCR(RT-PCR). Expression levels of the OsSalt gene decreased significantly in transgenic rice plants compared to control plant. However, ion leakage measurement did not demonstrate any leaves damage change between control and ArgE transgenic plants exposure to mannitol treatment. These results suggest that expression of the ArgE is not involved in tolerance for drought stress in rice but may playa role of signaling networks for salt-induced genes.
Agrobacterium-mediated Transformation of Rice 'Ilmibyeo' using HPT Selection Maker Gene
Guo, Jia,Cho, Joon-Hyeong,Jo, Hye-Jeong,Seong, Eun-Soo,Wang, Myeong-Hyeon The Plant Resources Society of Korea 2007 한국자원식물학회지 Vol.20 No.3
This study was conducted to produce the transgenic plant of rice. We obtained Agrobacterium AGL1 harbaring pCambial 300 vector with HPT gene. We carried out PCR analysis of 22 ea putative transgenic rice to investigate transformed lines. The 3 ea transgenic lines were detected insertion of HPT gene. Transgenic lines selected from PCR analysis were performed by Southern blot. From Southern blot, we obtained that two transgenic lines detected single band. We are going to study the method improving of cotransformation as well as transformation efficiency in rice.
Jia Guo,왕명현 한국원예학회 2011 Horticulture, Environment, and Biotechnology Vol.52 No.1
A dehydration responsive element binding (DREB) gene, designated LeDREB2, was isolated from tomato. It was classified as an A-2 group member of the DREB family. LeDREB2 is a single copy gene in the tomato genome, and it was strongly expressed in young leaves and roots but weakly expressed in mature leaves and shoots. Transcripts of the LeDREB2 genes were induced by various environmental stresses such as drought and cold. Expression analysis demonstrated that various oxidative stresses (e.g., salt (NaCl), abscisic acid (ABA), hydrogen peroxide (H2O2), methyl viologen (MV)) could induce the transcripts of LeDREB2 gene after application of time courses treatments except ABA. These results indicate that the LeDREB2 gene is a DREB transcription factor, which may play a role in both abiotic and oxidative stress responses.
A Parallel Method of Deep Packet Inspection based on Message-Passing Interface
Jia-xing Qu,Guo-yin Zhang,Xi-zhong Wang,Jia-hui Liu,Da-hua Song 보안공학연구지원센터 2015 International Journal of Security and Its Applicat Vol.9 No.12
With the increasing number of cores in multicore processors, it is challenging task how to take advantage of powerful parallel computing for the deep packet inspection. This paper introduces the deep packet inspection with a parallel method which exploits the message-passing interface (MPI). The parallel procedure includes the master thread and the slave thread. The master assigns the data packet to the slave. The slave executes the string matching with rules for inspecting. Both the master and the slave communicate by using MPI functions. The experimental results show that the parallel method is suitable for the trend of the increasing number of cores in multicore processors. Moreover, when the number of threads is equal to the number of cores in multicore processors, the performance arrives at the maximum throughput.
Modified GMDH networks for oilfield production prediction
Jia Guo,Wei Huang,Qiong Mao,Xudong Wang,Xinying Wang,Tao Song 한국자원공학회 2018 Geosystem engineering Vol.21 No.4
The self-organizing Group Method of Data Handling (GMDH) functional network is effective in predicting oilfield production. During operation the division of data sample depending on artificial classification cannot lead to global optimum in great probability and the variables are probably eliminated early in the iterative process in traditional GMDH algorithm. Recent years, GMDH model has been improved through many artificial intelligent models, but few people take the optimization of the model structure into account. In this paper, different training and testing set grouping and the effects of variables transmission were studied. The modified GMDH algorithm was optimized using the original variables preservation method and the random sample method, which was applied to the oilfield production forecasting simulation. The results of the modified GMDH algorithm, the traditional GMDH algorithm, ANNs and the empirical equations for predicting annual oil production were compared. The simulative results indicated that the modified GMDH model was the best tool for data-fitting with lowest error (RMSE = 13.9440, MAPE = 0.1121 and SI = 0.0378) and highest accuracy (R = 0.9984).