MicroRNA 452 regulates ASB8, NOL8, and CDR2 expression in colorectal cancer cells

Ji‑Su Mo,Soo‑Cheon Chae 한국유전학회 2021 Genes & Genomics Vol.43 No.1

Background MicroRNAs play important roles in the pathogenesis of human diseases by regulating target gene expression in specifc cells or tissues. Previously, we identifed microRNA 452 (MIR452), which was specifcally up-regulated in early stage human colorectal cancer (CRC) tissue. Objective The current study aims to identify and verify the target genes of MIR452 associated with CRC. Methods A luciferase reporter system was used to confrm the efect of MIR452 on ASB8, NOL8, and CDR2 expression. The expression levels of MIR452 and the target genes were evaluated by quantitative RT-PCR (qRT-PCR) and western blotting. Results We verifed the association between MIR452 and three genes, ASB8, NOL8, and CDR2, and showed that their transcripts were down-regulated by MIR452. Up-regulated MIR452 also down-regulated ASB8, NOL8, and CDR2 mRNA and protein levels in CRC cells. CDR2 protein expression was decreased in CRC tissues compared to adjacent non-tumor tissues. Conclusions These results suggest that ASB8, NOL8, and CDR2 were target genes of MIR452 in CRC cells and that upregulated MIR452 in CRC tissue regulated ASB8, NOL8, and CDR2 expression during colorectal carcinogenesis.

한국인 정상교합자의 3차원 디지털 모형을 이용한 순,협측 치면 곡률

채지현,송진욱,차정열,최정수,박영철 대한치과교정학회 2008 대한치과교정학회지 Vol.38 No.2

본 연구는 한국인의 치아형태에 적합한 브라켓 베이스를 만들기 위해 임상 치관의 순, 협측 곡률을 3차원적으로 계측하여 치관의 곡률 자료를 얻고자 하였다. 정상적인 해부학적 형태를 가지는 구강모형 30쌍을 3차원 디지털 모델로 제작한 후, FA점을 기준으로 수평 기준면과 수직 기준면을 설정하였다. 이 기준면에 대한 치면의 곡선을 형성한 후, 곡률을 이차방정식의 계수로 표현하였다. 수평면과 수직면의 곡률과 FA점을 기준으로 근심과 원심, 치은측과 교합면측의 곡률을 측정한 결과, 상악견치와 상악 제2소구치 외에는 남녀간 치면 곡률의 유의한 차이가 없었다. 하악 측절치, 상악 견치, 상하악 제1, 2소구치와 제1대구치에서 근원심 또는 치은교합면측 치면 곡률의 유의한 차이가 관찰되었다 (P < 0.05). 본 연구에서 얻어진 순, 협측 치면의 곡률 자료는 한국인의 치아 형태에 적합한 Straight Wire Appliance 개발의 기초 자료로 활용될 수 있다. Objective: This study was performed to investigate the labio/buccal clinical crown curvatures of Korean permanent teeth and to obtain the curve-ratio data in an attempt to fabricate bracket bases fit for each individual Korean permanent tooth. Methods: Three-dimensional digital models were made from 30 sets of dental casts with normal anatomic structures. According to the FA points, horizontal and vertical reference planes were established and lines were drawn on the tooth surfaces in reference to these planes. The curvature was expressed as the coefficient of a quadratic equation. Lines mesial, distal, gingival and occlusal to the horizontal, vertical reference planes and the FA point were drawn. Results: The curvature measured for each line revealed that there are no significant differences between male and female, except for maxillary canines and maxillary second bicuspids (P > 0.05). There were notable differences in the mesio-distal or gingivo-occlusal curvatures among the mandibular lateral incisors, maxillary canines, maxillary and mandibular first and second bicuspids and first molars (P < 0.05). Conclusions: The labial & buccal crown curvatures of teeth in Korean normal occlusion were measured on the mesial and distal, gingival and occlusal sides respectively in this study. Based on these data, a SWA can be developed to fit the individual features of Korean tooth crowns.

소모성 질환에 이환된 이유자돈에 대해 Porcine cytomegalovirus DNA Polymerase 유전자 검출과 분석

김지언,김지영,楚佳奇,채미경,장경수,김명철,박창식,전무형 충남대학교 수의과대학 동물의과학연구소 2004 動物醫科學硏究誌 Vol.12 No.-

Porcine cytomegalovirus (PCMV) causes piglet deaths, runting, inclusion body rhinitis, respiratory and reproductive disorders in swine herd, resulting in the significant economic losses in the swine industry. Recently, in the field of pig-to-human xenotransplantation, PCMV has been indicated as a potential xenogeneic pathogen with the risk for infection transmitted from the xenograft donor to the recipient. In this study, PCMV DNA polymerase gene was detected in the sera and nasal swabs from 30 heads of the weaned piglets affected with various wasting diseases and 10 heads of normal healthy piglets using polymerase chain reaction (PCR), and the diagnostic efficacy of PCR was evaluated. The prevalence of the PCR-positive by specimens from the diseased piglets were 83.3% in sera and 33.3% in nasal swabs. In the normal healthy group, the PCR-positive rates of sera and nasal swabs were 40% and 30%, respectively. Ten piglets (25.0%) out of 40 showed the simultaneously positive results in both of sera and nasal swabs. According to this result, it was found that the serum specimen was the most appropriate sample for detection of PCMV by PCR. The nucleotide sequence homology of DNA polymerase gene of PCMV KS1 as compared with the reference strain of PCMV (GenBank accesson no.AF027217) was 99.0%.

Roxythromycin-tylosin-furaltadone 합제의 닭 유래 세균에 대한 시험관내 항균효과

김지영,채미경,윤효인,서상희,박배근,김현수,전무형 충남대학교 수의과대학 동물의과학연구소 2003 動物醫科學硏究誌 Vol.11 No.-

In vitro antimicrobial susceptibility of roxythromycin (Rx), tylosin (Tyl), erythromycin (EM) and furaltadone (Fur) was studied, either by the single type or the combinated type of Rx-Tyl-Fur (RTF) against E. co1i (30 strains), Staph. aureus (20 strains) and Mycoplasma gallisepticum (5 strains) that are the major pathogens in poultry farm. The paper disk diffusion and MIC tests were applied. In screening test by paper disk diffusion method, the single types of antibiotics showed the sensitivity against 55 strains of bacteria in order of Fur, EM, Rx and Tyl. The combinated RTF showed the higher rate of sensitivity (80∼83.3% at 64㎍/disk, 60-75% at 32㎍/disk) and wider range of growth inhibiting zone (19.6±3.7-24.8±4.7mm) than those of the single drugs. In MIC test, the sensitivity against the 55 strains appeared in order of Fur. EM, Rx and Tyl as similar to those of paper disk diffusion method. The MIC ranges of RTF were 0.125∼16㎍/ml for E. coli, 0.25∼16㎍/ml for Staph. aureus and 1-8㎍/ml for Myco. gallisepticum, showing evidently lower than those of the single types. The MIC_(50) and MIC_(90) of RTF were estimated l∼4㎍/ml and 8∼16㎍/ml, respectively, and remarkably lower than those of the single drugs.

일부 산업장 근로자들의 직업성 피부질환 및 천식의 유병조사

윤지석,이영수,조영채,이동배 충남대학교 의과대학 지역사회의학연구소 1990 충남의대잡지 Vol.17 No.2

For the analysis of prevalence of occupational skin diseases and asthma in industrial workers, we studied the 1,274 workers who were engaged in manufacturing workers(direct exposured group to harzardous materials) and clerical workers (indirect exposured group to harzardous materials). The results were as follows: 1. The prevalence rate of occupational skin diseases and asthma in manufacturing workers(36.4%) was significantly higher than that in clerical workers(25.2%) (p<0.005). 2. The prevalence rates of skin diseases(p<0.005) and asthma(p<0.05) in male manufacturing workers were significantly higher than those in clerical workers, and the prevalence rate of asthma(p<0.005) in female manufacturing workers was significantly higher than that in clerical workers. 3. Of asthma in twenties(p<0.005), of skin diseases(p<0.005) and asthma(p<0.05) in thirties, the prevalance rate of manufaturing workers were higher than thoses of clerical workers, however no diffrence was in forties. 4. Of skin diseases(p<0.005) and asthma(p<0.05) in metal plating, of asthma(p<0.005) in cotton spinning, the prevalence rate of manufacturing workers were significantly higher than those of clerical workers. 5. The prevalence rate of skin diseases and asthma in dustness part were significantly higher than those of non-dustness part and poor ventilation part also were significantly higher than good ventilation part (p<0.005). 6. Frequency of involved skin sites, in descending order, were hand(32.1%), leg(24.3%), trunk(20.4%), face(12.1%) and arm(11.1%).

승모판 혈류의 E파와 폐정맥혈류의 D파의 최대속도 간의 시간 간격과 승모판 도플러 지수들과의 관계

전두수,이만영,박지원,김용주,임효영,강동헌,이길환,김종진,채장성,김재형,홍순조,최규보 대한순환기학회 1999 Korean Circulation Journal Vol.29 No.9

경식도 심초음파도의 Direct Planimetry를 이용하여 측정한 대동맥판 면적의 심박출량 및 좌심실 구출율의 변화에 따른 정확도

진승원,김종진,김희열,박지원,전두수,윤호중,박준철,노태호,채장성,김재형,홍순조,최규보 대한순환기학회 2000 Korean Circulation Journal Vol.30 No.8

고추 탄저병 저항성 중간모본 ‘원예 100005호 ’,‘원예 100006호’ 육성

양은영,김수,채영,채수영,조명철,문지혜,박태성,이우문,곽정호 忠北大學校 農業科學硏究所 2015 農業科學硏究 Vol.31 No.2

Owing to frequent heavy rain due to abnormal weather, the occurrence of pepper anthracnose has recently increased, which affects the supply and demand of pepper. As a result, the price of red pepper powder in 2011, for example, increased three times compared to the average year. Although anthracnose of pepper plants has been controlled by fungicides until now, it is important to develop new resistant cultivars because of the reduction of the labor force in rural area and occurrence of new anthracnose strains resistant to fungicides. National Institute of Horticultural and Herbal Science developed two pepper lines, ‘Wonye 100005’ and ‘Wonye 100006’ that are resistant to pepper anthracnose. These lines were developed by continuous selection of progenies from a cross between ‘Wonye AR1’, which were developed from interspecific hybridization and ‘TF68’. The anthracnose resistance level of the two lines was higher than that of commercial cultivars and similar to check varieties showing highest level of anthracnose resistance.

노인의 영적 간호요구

최미혜,김경희,김귀옥,김기숙,김수강,김정신,김춘숙,노흥진,박지연,성혜연,오명선,이선희,이원옥,이윤영,이현수,장명재,차혜경,채정선,홍상희 중앙대학교 의과대학 간호학과 간호과학연구소 2001 중앙간호논문집 Vol.5 No.1

This study was designed to exam the aged's needs for spiritual nursing care. The purpose was to serve as a basis for the development of spiritual nursing practice. The major findings are as follows : 1. The degree of needs for spiritual nursing care as area was that needs of love and relationship mean 22.0, needs of meaning and object mean 28.2, needs of forgiving mean 13.5. Total needs for spiritual nursing care mean 63.7, which was on the upper middle level. The needs of meaning and object was rated highest. 2. Among the general characteristics of the subjects, needs of love and relatiohship wasn't significanlty different. 3. Among the general characteristics of the subjects, needs of meaning and object was significantly different according to two factors : age(F=7.260, p=0.001), religion(F=5.275, p=0.001). Higher needs of meaning and object was possessed by the older than the younger, by the one who have religion than the other. 4. Among the general characteristics of the subjects, four factors made a significantly difference to needs of forgiving : sex(t=-2.851, p=0.006), age(F=8.201, p=0.001), religion(F=6.928, p=0.000), disease(t=2.327, p=0.024). Higher needs of forgiving was possessed by man than woman, by the older than the younger, by the one who have religion than the other, by the one who have disease than the other.

Soil Microbial Characteristics of Different Pepper Cropping Types

Soo Young Chae,Eun Young Yang,Seon Hye Son,Myeong-Cheoul Cho,Young Chae,Yang Ji Seo 한국원예학회 2008 한국원예학회 기타간행물 Vol.- No.-