심근경색 모델 흰쥐에서 스트레스가 B-type Natriuretic Peptide 발현 및 심박변이율 변화에 미치는 영향

이삼윤(Sam Youn Lee),이미경(Mi Kyoung Lee),김남호(Nam-ho Kim),주민철(Min Cheol Joo),조항정(Hyang Jeong Jo),강지숙(Ji Sook Kang),김병숙(Byung Sook Kim),윤선식(Sun Sik Yoon),최을식(Eul Sig Choi),이문영(Moon Young Lee) 대한스트레스학회 2010 스트레스硏究 Vol.18 No.3

심박변이율의 감소는 심근 경색 환자에서 예후의 악화와 관련되어 있다. 심박변이율 중 very low frequency가 심부전환자의 예후를 판단할 수 있는 독립적 인자로서 사용이 가능하다고 보고된 바 있으며, 심실의 압력 증가에 반응해서 생성되는 B-type natriuretic peptide가 심근 경색의 예후 인자로서 사용할 수 있음 역시 보고된 바 있다. 본 연구에서는 심근경색 모델을 제작하여 일정의 회복 기간을 거친 다음 다시 일정 기간의 스트레스를 겪게 한 후 심박변이율의 변화 및 심실 조직에서의 BNP 발현 정도를 비교하여 스트레스에 의한 영향을 관찰하고자 하였다. Sprague-Dawley계 수컷 흰쥐 15마리를 대상으로 하였다. 실험동물은 인위적 스트레스 및 수술을 받지 않은 대조군(CON, n=5), 심근경색 수술을 받은 후 restraint stress를 받지 않은 군(MI+No Stress, n=5), 심근경색 수술을 받은 후 1주일 동안 restraint stress를 받은 군(MI+Stress, n=5)으로 분류하였다. 심근경색 모델 제작 직후 15분 이상 심전도의 변화를 기록하였으며, 2개월 정도 후 심근경색 수술을 받은 동물을 두 군으로 나누어 그 중 한 군에는 1주일 동안 restraint stress를 가한 후 restraint stress를 가하지 않은 동물과 심박변이율을 비교 분석하였다. 심실 조직은 좌심실 전벽 부위의 위축을 관찰할 수 있었고, 대식세포에 의하여 응고, 괴사된 심근세포의 탐식과 혈관이 풍부한 육아조직 및 섬유 변화를 볼 수 있었다. 심박변이율은 심근경색 수술을 받은 직후 심박수는 유의한 증가를 보였고, standard deviation of the normal to normal intervals (SDNN), very low frequency (VLF) 및 low frequency (LF)의 유의한 감소를 보였다. 심근경색 수술을 받았던 동물에서 두 달 정도의 회복 기간을 거친 후 스트레스를 받지 않은 군은 심박수 및 기타 심박변이율 분석에서 SDNN 값을 제외하고는 정상군과 유의한 차이를 관찰할 수 없었던 반면 1주일 동안 스트레스를 받은 군에서는 심박수가 다시 유의하게 증가했을 뿐만 아니라 SDNN, VLF 및 LF 역시 정상군과 유의한 차이를 보였다. 이상의 결과를 종합하면 심근경색 동물에 대해 1주일 동안 스트레스를 가한 결과 심박변이율이 감소하고 심실에서의 BNP 발현은 더욱 증가하여 악화함을 보여주고 있다. Diminished heart rate variability (HRV) is associated with less favorable prognosis after myocardial infarction (MI). It has been reported that very low frequency (VLF) power in HRV analysis is an independent risk predictor in patients with congestive heart failure and B-type natriuretic peptide (BNP) can be used as a prognostic factor of MI. The purpose of this study was to investigate whether stress has an effect to the changes of BNP expression and/or heart rate variability in MI model in rats. Male Sprague-Dawley rats were divided into 3 groups: control group (CON), MI group (MI+No Stress), and MI followed by stress group (MI+Stress). MI+Stress group rats were raised for a two month recovery period after the operation, followed by being exposed to restraint stress for 2 hours per day for 1 week. Electrocardiogram was recorded after the operation and the last day after 1 week of stress. The frequency components of HRV were calculated in the frequency domain such as VLF, low frequency (LF), high frequency (HF) and so on. In HRV analysis, standard deviation of the normal to normal intervals (SDNN) was significantly reduced in both groups compared to the control group. VLF and LF also were significantly reduced in MI+Stress group compared to the control groups. In addition, BNP expression in western blotting was shown the strongest bands in MI+ Stress group among experimental groups. These results suggest that BNP and HRV were aggravated by stress in MI rat model. (Korean J Str Res 2010;18:275∼285)

토양환경지도 자료를 이용한 콩밭 토양의 화학성 비교

박지숙,이민진,정재원,김미혜,이서연,지윤미,한준호,김유학,공명석,한광현,노희명 忠北大學校 農業科學硏究所 2015 農業科學硏究 Vol.31 No.2

It is important to interpret the soil chemical properties in order to effectively manage the farmland. This study was conducted to investigate the soil chemical properties of upland soybean field according to the soil series at 32 upland fields located in the parts of Gangwon-do. Soil sampling sites were selected by using the National Statistics DB and KSIS DB that is a soil environment information system provided by the Rural Development Administration. Soil samples were collected from 0~15 cm of top soil before seeding or transplanting, air-dried, passed through a 2 mm sieve, and analyzed for soil pH, EC, organic matter, available phosphorus (P2O5), exchangeable potassium, exchangeable calcium and exchangeable magnesium. The average chemical properties have exceeded the each nutrient optimum range level of RDA, except the organic matter in experimental soil 2015. And despite application of conventional fertilization, each soil series nutrient contents were change respectively. The results of this study suggest that conventional fertilization should be applied based on the analysis about the amount of accumulated nutrient contents in the soil.

국내 캐주얼 브랜드의 남성복 의류 생산현황 및 치수체계 실태에 관한 연구

윤지원;서미아 한양대학교 2007 韓國 生活 科學 硏究 Vol.27 No.1

casual wear, sizing system, obese menThe purpose of this study is to provide reference material for establish sizing system through investigation of status of production and sizing system about domestic ready to fit casual brand. For this study, status of casual wear production and sizing system was examined through the survey of 13 domestic casual wear brand. The result of survey of casual wear brand showed that there was lack of unification between each company due to that each brand produced with different size code and same size code produced with different dimensions between each company. And systematic information based on actual body measurement data was not available due to lack of knowledge about body size in determination of standard size or product size. therefore it is considered that standard apparel sizing system is required for effective casual wear production because consumer's demand to wear more fitted clothes is not satisfied and especially sizing system for obese men is not covered.

요통환자의 요부 안정화 운동 방법이 통증감소에 미치는 효과에 대한 연구

조미숙,최윤희,김경,이지연,김진섭 대구대학교 특수교육재활과학연구소 2010 再活科學硏究 Vol.28 No.1

요통이란 근 골격의 이상으로 인한 통증으로 흉추 10번 이하의 허리부위에서 통증이 3일 이상 지속되는 경우를 말하며 인구의 약80% 이상이 일생을 통해 적어도 한번쯤은 겪게 된다. 이러한 요통은 체간의 연부조직의 손상이나 근력의 약화가 주요원인으로 작용하며, 이로 인해 통증, 지구력 감소, 관절가동범위 제한, 보행능력 감소를 유발한다. 이는 요통과 운동이 많은 관련성을 가지고 있다고 사료되며 그 중 요부 안정화 운동이 요통 환자의 통증 감소에 미치는 효과에 대해 선행연구들을 살펴봄으로써 효과적인 요부 안정화 운동방법과 적용에 도움을 주고자한다. Damage of soft tissue in lumbar interbody and weakness of muscular strength are the major causes of lower back pain, which results in inducing pain sensation, reducing muscular endurance and flexibility, limiting range of motion, and lowering locomotive capacity. Lower back pain is considered to be closely related to exercise. Exercise intervention in patients with low back pain being used as one of the spinal stabilization exercises and low back pain patients that exercise is effective in reducing the pain has been reported. Therefore, patients with low back pain of spinal stabilization exercises for pain reduction, previous studies on the effect of spinal stabilization exercise by examining how effective and will give help to apply.

노인의 영적 간호요구

최미혜,김경희,김귀옥,김기숙,김수강,김정신,김춘숙,노흥진,박지연,성혜연,오명선,이선희,이원옥,이윤영,이현수,장명재,차혜경,채정선,홍상희 중앙대학교 의과대학 간호학과 간호과학연구소 2001 중앙간호논문집 Vol.5 No.1

This study was designed to exam the aged's needs for spiritual nursing care. The purpose was to serve as a basis for the development of spiritual nursing practice. The major findings are as follows : 1. The degree of needs for spiritual nursing care as area was that needs of love and relationship mean 22.0, needs of meaning and object mean 28.2, needs of forgiving mean 13.5. Total needs for spiritual nursing care mean 63.7, which was on the upper middle level. The needs of meaning and object was rated highest. 2. Among the general characteristics of the subjects, needs of love and relatiohship wasn't significanlty different. 3. Among the general characteristics of the subjects, needs of meaning and object was significantly different according to two factors : age(F=7.260, p=0.001), religion(F=5.275, p=0.001). Higher needs of meaning and object was possessed by the older than the younger, by the one who have religion than the other. 4. Among the general characteristics of the subjects, four factors made a significantly difference to needs of forgiving : sex(t=-2.851, p=0.006), age(F=8.201, p=0.001), religion(F=6.928, p=0.000), disease(t=2.327, p=0.024). Higher needs of forgiving was possessed by man than woman, by the older than the younger, by the one who have religion than the other, by the one who have disease than the other.

콜리플라워를 이용한 피클제조 최적화

정현아,윤지영,황재선,주나미 한국식생활문화학회 2004 韓國食生活文化學會誌 Vol.19 No.2

The purpose of this study was to determine the organoleptic characterisitcs of cauliflower pickles made in various compounding ratio according to central conposite design for optimum organoleptic characteristics of the cauliflower pickles. The optimum mixing condition of cauliflower pickles were optimized, using central composite design with 3 variables and 3 levels, by response surface methodology. The various kinds of cauliflower pickle were made in various compounding ratio of vinegar, salt and sucrose - critical ingredients of pickle recipe - and were presented to reliable panels, who graded the subjects in 7 degrees for 4 items : color, lavor, hardness and overall quality. The optimum mixing conditions of cauliflower pickle were 603.50g of vinegar, 80.13g of salt and 251.079 of sucrose in the maximum point of overall quality.

茶 침출조건에 따른 Catechin, 무기질 및 Vitamin C 함량 변화

이순재,김미지,윤연희,박규영,최원경,방진숙,부용출 대구효성가톨릭대학교 응용과학연구소 1994 응용과학연구논문집 Vol.3 No.-

본 연구에서는 실제 상용하고 있는 각 차음료가 온도, 시간 및 추출 횟수에 따른 성분의 함량의 차이를 관찰코져 시판되고 있는 한국산 녹차, 우롱차 및 홍차를 대상으로 추출방법을 달리한 후 茶浸出液중의 주요성분인 catechin, vitamin C 및 여러가지 무기질의 함량을 측정하여 다음과 같은 결과를 얻었다. 1. 차침출온도에 따른 침출액중의 catechin과 vitamin C 함량의 차이를 볼 때 온도가 높을수록 많이 추출되었다. 2. 같은 온도라는 침출시간에 따른 차이를 관찰한 결과 시간이 길수록 침출액중의 catechin과 vitamin C의 함량이 증가되었다. 3. 녹차는 우려낸 횟수에 따른 catechin의 함량 차이는 처음에 비하여 2회 추출시약 51%, 3회추출시는 71% 감소하였다. 4. 차의 각 무기원소에 따른 함량을 비교시 Mg은 우롱차가 가장 높고 K, Mn은 녹차가, Zn, Cu 그리고 Fe는 홍차가 가장 높게 나타났다. 5. 녹차, 우롱차의 경우 대체로 무기질의 침출정도가 온도가 높을수록 오히려 감소하는 경향을 나타내었고 홍차는 Mg, K을 제외하고 모두 증가하였다. 6. 각 침출액중의 대부분의 무기질 함량은 추출횟수가 증가할수록 감소하였다. 7. 시료로 사용한 녹차, 우롱차, 홍차의 vitamin C 함량은 녹차, 우롱차, 홍차의 순으로 높게 나타났다. 8. 녹차를 추출한 횟수에 따른 vitamin C 함량은 1회에 비해 2회 추출시 약 36%, 3회 추출시 약 62% 감소되었다. 9. 5g의 녹차를 80℃에서 2분간 침출한 액중에서는 catechin은 100ml당 0.34g, Mg은 2.229mg, Ca은 0.365mg, K은 39.77mg, Zn은 0.037mg, Cu은 0.010mg, Fe는 0.001mg, Mn은 0.581mg이며, vitamin C는 9.65mg이 함유되어 있었다. This study was to compare the ingredients which are contained in usual Korean teas in-depednence on leaching temperature, time and number. The main contents i.e. catechin, vi-tamin C and other minerals extracted from Korean commercial green tea, oolong tea and black tea were measured. The resluts are as follows; According to extracting temperature, the higher temperature made catechin and vitamin C extracted more. For the same leaching temperature, the longer time the more catechin and vitamin C. In case of extracting number, the contents of catechin was decreased a-bout 50% and 70% at second time and third, respectively in comparasion to the first time. For minerals from teas, management(Mg) was most in oolong tea, potassium(K)and manganease(Mn) and in green tea and iron(FEe) was in black tea respectively. Most min-erals from greentea and oolong tea were extracted less as leaching temperature was in-creased. But in black tea minerals other than Mg and K were more at increased tempera-ture. Most minerals in extractions were decreased as leaching number was increased. The content of vitamin C in the experimental samples(tea) was most in green tea, next in oo-long tea and third in black tea. In comparasion with the first extraction vitamin C was de-creased about 35% and 60% at 2nd and 3rd leach, respectively.

B16 melanoma 세포에서 nitric oxide가 멜라닌 생성에 미치는 영향

심상수,조남영,윤미연,이지윤,김창종 중앙대학교 약학연구소 2002 약학 논총 Vol.16 No.-

To investigate the effects of nitric oxide and cGMP on melanin production, we measured the melanin production in B16 melanoma cells stimulated by alpha-melanocyte stimulating hormone (MSH). NO donor, such as, sodium nitroprusside (SNP), SIN-1, SNAP and L-arginine increased melanin production in basal state. SNAP potentiated melanin production by MSH, but other NO donors did not. In the study the potentiating effect of sodium nitroprusside on cAMP-dependent melanin production, sodium nitroprusside did not increase melanin production induced by MSH, forskolin and 8-Br-cAMP. Non-selective cAMP/cGMP phosphodiesterase inhibitor (IBMX) and selective cGMP phosphodiesterase inhibitor (zaprinast) significantly increased MSH-induced melanin production, whereas soluble guanylyl cyclase inhibitor (ODQ) inhibited MSH-induced melanin production. These results suggest the possibility that MSH-stimulated melanin production may be mediated via not only cAMP-dependent pathway but also cGMP-dependent pathway in B16 melanoma cells.

여성농업인 노동의 경제적 가치평강 관한 연구 : 농업노동과 가사노동 Agricultural Work and Household Work

유소이,최윤지,조현숙,김경미 대한가정학회 2003 Human Ecology Research(HER) Vol.41 No.7

The purposes of this study were to explore the rural women's labor by measuring labor value economically. To achieve the purpose, this study applied four methods : replacement cost method individual function, replacement cost method generalist, opportunity cost method and shadow wage method. The results of this study were as follows; 1) Time used for agricultural work and household work by rural women were 5.3 hours and 3.8 hours each. 2) According to the methods used, the amounts of valuing rural women's labor were varied and ranged from 23,000 won to 43,000 won per day. This study might help recognize the degree of rural women's labor contribution to the household income of farm households and improve the socio-economic status of rural women through showing the productivity of the rural women's labor.

수행 평가 도구를 이용한 심화·보충형 수준별 교수 전략 개발 : 과학 3의 물질의 구성 Composition of Matter Section in Science 3

강순희,김지영,박은미,이윤하 이화여자대학교 사범대학 교과교육연구소 2005 교과교육학연구 Vol.9 No.2

제7차 교육 과정의 과학 교과에 대한 심화·보충형 수준별 수업 전략을 개발하여 현장 과학 교사들에게 구체적인 도움을 주고자 하는 것이 본 연구의 목적이다. 수업 운영 형태는 동일 학급 내 교과 수준별 분단 편성이며, 연구된 구체적인 단원은 중학교 과학 3의 ‘물질의 구성’ 단원으로 총 17차시가 소요된다. 먼저 현실적으로 중등 과학 교육 현장에서 실현 가능한 과학과 수준별 교육 과정에 관련된 이론적 배경을 고찰하였다. 둘째, ‘물질의 구성’ 단원에 대하여 차시 수업의 목표로 사용 가능한 구체적인 성취 기준을 지식, 탐구, 태도 영역으로 개발하였다. 셋째, 앞에서 개발한 성취 수준의 도달 정도를 판별하는 평가 기준을 개발하고, 이 성취 수준과 평가 기준을 토대로 하여 동일 학급 내 과학 교과 수준별 분단 편성을 위한 진단 평가 도구들을 개발하였다. 넷째, 이 진단 평가 도구에 의하여 학생들을 수준별 소집단 분단으로 분류하는 구체적인 방법을 제시하였다. 마지막으로, 각 동일 학급 내 수준별 분단에 사용할 수 있는 보충 활동지를 개발하였으며 사용하는 방법을 제시하였다. The purpose of this study is to development of instructional strategy for differentiated curricular by enrichment and supplementary instructions about junior high school science under the 7th National Curricular Revision. The ability grouping for differentiated curricular in this study was within class ability grouping in same year. The composition of matter in junior high school science 3 was selected for this study and analyzed to develope the detailed achievement standards and assessment standards of school class level for science teacher. Based on those detailed achievement standards and assessment standards of school class level, the concept clusters of the composition of matter section was classified. The diagnostic evaluation which was the performance assessment tools according to the concept clusters was developed to classify for enrichment and supplementary groups. Also the method of classification about enrichment and supplementary groups in science class was suggested in details. Finally, teaching and learning materials for supplementary groups according to the concept clusters were developed about the composition of matter section in science 3 at junior high school.