全蝎 抽出物이 血栓症, 全腦虛血 및 腦細胞毒性에 미치는 影響

백명현,황영근,정지천,강정준,김성훈 동국대학교 한의학연구소 1999 東國韓醫學硏究所論文集 Vol.8 No.1

본 실험은 전갈 추출물이 어혈병태 모델과 KCN으로 유발된 전뇌허혈, 뇌세포독성 등에 미치는 영향을 관찰하였다. 전갈은 Dextran에 의해 유도된 혈전으로 감소된 혈소판수, fibrinogen량, hemocrit치를 유의성 있게 증가시키고. 증가된 prothrombin time을 유의성 있게 단축시켰다. 전갈은 thrombin과 ADP에 의해 유도된 혈소판 응집을 억제하였으나 collagen에 의해 유도된 혈소판 응집에는 저해효과를 나타내지 않았다. 또한 collagen과 epinephrine에 의해 유도된 pulmonary embolism에 대하여 보호 효과를 나타내었다. 전갈은 KCN에 의한 전뇌허혈 유발 실험에서 혼수시간을 유의성 있게 단축시켰고, Amyloid β protein(25-35)에 의해 유도된 PC12 세포의 독성에 대하여 보호효과를 나타내었다. This following is effect of Buthus martensi Karsch (BMK) extract on dextran-thrombus model, KCN-induced coma, cytotoxicity of brain etc. BMK extract significantly increased number of platelet and fibrogen and significantly shortened the prothrombin time as compared with control group treated with dextran. BMK extract didn't affect the changes of hematocrit as compared with control group treated with dextran. BMK extract induced a significant inhibition of human platelet aggregation induced by thrombin and ADP but did not affect human platelet aggregation induced by collagen. BMK extract showed a protective effect on pulmonary thrombosis induced by collagen and epinephrine. BMK extract prolonged the duration of KCN-induced coma and showed a protective effect on cytotoxicity of PC12 cells induced by amyloid β protein(25-35) in a dose dependent manner. These results suggested that BMK extract might be usefully applied for prevention and treatement of thrombosis and brain damage.

升麻葛根湯 및 升麻葛根湯合四物湯의 抗 Histamine 效果에 關한 硏究

金賢兒,鄭智天,朴宣東 동국대학교 한의학연구소 1994 東國韓醫學硏究所論文集 Vol.3 No.-

In order to find out the effect of Anti-Histamine of the Sungmagalkuntang and Sungmagalkuntang-plus-samultang I've investigated the effect of the time to death, the peripheral vascular Peaneability, the amount of secretion of Gastric Juice, the total Acidity of Gastric Juice, the contraction of Intestine Tube by Histarnine and the effect of the Skin-Reaction by the decrease of DNCB. These results are following 1. The effect of Mice s rime to death by Histamine was that the injection group of sungmagalkuiigtang and the injection group of sungmagalkungtang-plus-samultang both have had the effect of the extention uf the time lo death, which indicated a statistical significance in comparison with comparative group. 2. The effect of the increased vascular permeability by Histamine was that the injection group of sungmagalkuntang and the injection group of sungmagalkuntang-plus-samultang both have had the effect of the restraint of the increased vascular permeability, which indicated a statistical significance in comparison with comparative group. 3. The effect of the the amount of the secretion of Gastric Juice by Histamine stimulus was tirat the injection group of sungmagalkuntang-plus-samultang only has had the effect of restraint of the increased of the secretion of Gastric Juice, which indicated a statistical significance in comparison with comparative group. 4. The effect of the chan,ge of total Acidity of Gastric Juice by Histamine's stimulus was that the injection group of sungmagalkuntang-plus-samultang only has had the effect of the restraint of the increase of tolal Acidity of Gastric Juice,, which indicated a statistical significance in comparison with comparative group. 5. The effect of the contraction of Intestine Tube by Histamine was that sungmagalkuntang and sungmagalkuntang-plus-samultang both have had the effect of the contraction and the restraint According to the increase of the density, the effect of the restraint was more clear. But the difference of the effects between two substances had little. 6. The effect of the reaction of skin by the decrease of DNCB was that the injection group of sungmagalkuntang and the injection group of sungmagalkuntang-plus-samultang have had the decreased effect of the Skin-Reaction in comparison with comparative group. sungmagalkun-tang-plus-samultang aws more effective. In these results shown above, both sungmagalkuntang and sungmagalkuntang-plus-samultang was shown as more effective one

鹿脛骨이 卵巢摘出로 誘發된 흰쥐의 骨多孔症에 미치는 影響

姜智天,金頀哲,林康鉉,安德均 대한본초학회 1999 大韓本草學會誌 Vol.14 No.2

The purpose of this study is to examine what are the effects of the Deer Tibia on the postmenopausal osteoporosis. In order to observe the effects of the Deer Tibia on the osteoporosis induced from ovariectomy, administered the Deer Tibia in p.o to the ovariectomized Rats, and measured the variance of weight related to osteoporosis, the variance of osteocalcin, calcium, ALP, phosphorus, GOT and GPT in serum, the variance of creatinine, calcium to creatinine rate in urine and urinary volume, the variance of the organs in weight and the variance of the bones. The results of the experiment are as follows: 1. The level of serum ALP, S-GOT and S-GPT in Deer Tibia group was showed a significant decrease in comparison with control group. The level of serum osteocalcine, calcium, phosphorus in Deer Tibia group was showed no efficiency in comparison with control group. 2. The level of urinary volume in Deer Tibia group was showed a significant increase in comparison with control group, and calcium to creatnine ratio in Deer Tibia group showed a significant increase. 3. The weight of tibia ash and the length of femur in Deer Tibia group showed no significant change. The bone density in Deer Tibia group showed a significant increased in comparison with control group. 4. Body weight in Deer Tibia group was increased in comparison with control group, but showed no efficiency. The weight of heart, liver, spleen, kidney and uterus in Deer Tibia group showed no significant change in comparison with control group. According to the above results, effects of the Deer Tibia on the aged ovariectomized rat model showed a efficiency in elevation of bone density by increasing bone mass and in prevention of renal function failure by ovariectomy. So Deer Tibia can be used for prevention and curing of the postmenopausal osteoporosis, as for it's effects, continuous clinical study is needed.

아므르불가사리 (Asterias amurensis)의 배우자를 이용한 남해해역 연안해수의 생물학적 수질평가

유춘만,박지인,조기안,주현수,박종천,나명석 한국환경과학회 1999 한국환경과학회지 Vol.8 No.1

This study was carried out to evaluate off-shore water quality and to draw marine pollution map to Southern sea in Korea by bioassay using gametes and early development system of Asterias amurensis. From the bioassay, it was determined that the water qualites of Southern sea maintained the grade Ⅱ, because development of A. amurensis were moderately inhibited. However, Sunchon, Kwangyang, and Jinhae Bay belonged to grade Ⅲ, because development of A. amurensis were strongly inhibited. This could be due to the inflows of several river systems and poor water circulation. The water quality at Yosu island was the grade I, because A. amurensis were not inhibited in its early development.

Pyruvate Kinase M2 : A Novel Biomarker for the Early Detection of Acute Kidney Injury

Ji Hyun Cheon,Sun Young Kim,Ji Yeon Son,Ye Rim Kang,Ji Hye An,Ji Hoon Kwon,Ho Sub Song,Aree Moon,Byung Mu Lee,Hyung Sik Kim 한국독성학회 2016 Toxicological Research Vol.32 No.1

The identification of biomarkers for the early detection of acute kidney injury (AKI) is clinically important Acute kidney injury (AKI) in critically ill patients is closely associated with increased morbidity and mortality. Conventional biomarkers, such as serum creatinine (SCr) and blood urea nitrogen (BUN), are frequently used to diagnose AKI. However, these biomarkers increase only after significant structural damage has occurred. Recent efforts have focused on identification and validation of new noninvasive biomarkers for the early detection of AKI, prior to extensive structural damage. Furthermore, AKI biomarkers can provide valuable insight into the molecular mechanisms of this complex and heterogeneous disease. Our previous study suggested that pyruvate kinase M2 (PKM2), which is excreted in the urine, is a sensitive biomarker for nephrotoxicity. To appropriately and optimally utilize PKM2 as a biomarker for AKI requires its complete characterization. This review highlights the major studies that have addressed the diagnostic and prognostic predictive power of biomarkers for AKI and assesses the potential usage of PKM2 as an early biomarker for AKI. We summarize the current state of knowledge regarding the role of biomarkers and the molecular and cellular mechanisms of AKI. This review will elucidate the biological basis of specific biomarkers that will contribute to improving the early detection and diagnosis of AKI.

Pyruvate Kinase M2: A Novel Biomarker for the Early Detection of Acute Kidney Injury

Cheon, Ji Hyun,Kim, Sun Young,Son, Ji Yeon,Kang, Ye Rim,An, Ji Hye,Kwon, Ji Hoon,Song, Ho Sub,Moon, Aree,Lee, Byung Mu,Kim, Hyung Sik Korean Society of ToxicologyKorea Environmental Mu 2016 Toxicological Research Vol.32 No.1

The identification of biomarkers for the early detection of acute kidney injury (AKI) is clinically important. Acute kidney injury (AKI) in critically ill patients is closely associated with increased morbidity and mortality. Conventional biomarkers, such as serum creatinine (SCr) and blood urea nitrogen (BUN), are frequently used to diagnose AKI. However, these biomarkers increase only after significant structural damage has occurred. Recent efforts have focused on identification and validation of new noninvasive biomarkers for the early detection of AKI, prior to extensive structural damage. Furthermore, AKI biomarkers can provide valuable insight into the molecular mechanisms of this complex and heterogeneous disease. Our previous study suggested that pyruvate kinase M2 (PKM2), which is excreted in the urine, is a sensitive biomarker for nephrotoxicity. To appropriately and optimally utilize PKM2 as a biomarker for AKI requires its complete characterization. This review highlights the major studies that have addressed the diagnostic and prognostic predictive power of biomarkers for AKI and assesses the potential usage of PKM2 as an early biomarker for AKI. We summarize the current state of knowledge regarding the role of biomarkers and the molecular and cellular mechanisms of AKI. This review will elucidate the biological basis of specific biomarkers that will contribute to improving the early detection and diagnosis of AKI.

Highly Halaven-resistant KBV20C Cancer Cells Can Be Sensitized by Co-treatment with Fluphenazine

CHEON, JI HYUN,LEE, BYUNG MU,KIM, HYUNG SIK,YOON, SUNGPIL Potamitis Press 2016 Anticancer research Vol.36 No.11

<P>Aim: To identify conditions that induce an increase in the sensitivity of highly Halaven (HAL)-resistant cancer cells compared to sensitive cells. Materials and Methods: We observed that drug-resistant KBV20C cells are highly resistant to HAL compared to other antimitotic drugs. The concentration required to treat KBV20C cells was almost 500-fold higher than that used to treat sensitive parent KB cells. In order to increase sensitization, HAL-treated KBV20C cells were co-treated with the repositioned drug, fluphenazine (FLU). Results: HAL and FLU co-treatment inhibited the growth and increased apoptosis via G(2) arrest in HAL-treated KBV20C cancer cells. Sensitization by HAL-FLU affected retinoblastoma protein (pRB), pHistone H3 and pH2AX protein levels. FLU could also inhibit p-glycoprotein (P-gp) activity in HA-resistant KBV20C cells. These observations suggest that the mechanisms underlying FLU-HAL sensitization in resistant KBV20C cells involve both apoptosis and P-gp inhibition. Furthermore, both thioridazine (THIO) and mefloquine (MEF), but not azathioprine (AZA), sensitized HAL-treated KBV20C cells. Conclusion: These findings provide important information regarding the sensitization of HAL-resistant cells and indicate that FLU, THIO and MEF may have similar sensitization effects in highly HAL-resistant cells.</P>

Use of Phenolic Foam as a Medium for Production of Plug Seedlings of ‘Superdotaerang’ Tomato

Ji Hyun Lee,Jung Ji Park,Ji Eun Park,Jae Sun Jeong,Sang Hyun Park,Young Jin Cheon,Kwang Soo Kim,Byoung Ryong Jeong,Seung Jae Hwang 한국원예학회 2010 한국원예학회 학술발표요지 Vol.2010 No.10

The effect of a steamed matured silkworm and black ginseng to prevent weight gain in dogs

Ki Hyun Kim,Jung Hwan Jeon,Sang Yun Ji,Min Ji Kim,Ji Yeon Jung,Si Nae Cheon,Geum Zoo Yoo,Dong Hoon Kim,Ju Lan Chun 한국실험동물학회 2020 한국실험동물학회 학술발표대회 논문집 Vol.2020 No.7

삼십 년 동안의 치루를 동반한 장결핵

천지현 ( Ji Hyun Cheon ),문원 ( Won Moon ),박선자 ( Seun Ja Park ),박무인 ( Moo In Park ),김성은 ( Sung Eun Kim ),최윤정 ( Youn Jung Choi ),김종빈 ( Jong Bin Kim ),권혜정 ( Hye Jung Kwon ) 대한소화기학회 2013 대한소화기학회지 Vol.62 No.6

Tuberculosis can occur anywhere in the gastrointestinal tract. However, anorectal tuberculosis has rarely been reported. A 46-years-old male presented with abdominal pain and perianal discharge of 30 years` duration. The patient had received operations for anal fistula and inflammation three times. Although he had been taking mesalazine for the past three years after being diagnosed with Crohn`s disease, his symptoms persisted. Colonoscopy performed at our hospital revealed cicatricial change of ileocecal valve and diffuse ulcer scar with mild luminal narrowing of the ascending, transverse, and descending colon without active lesions. Multiple large irregular active ulcers were observed in the distal sigmoid and proximal rectum. An anal fistula opening with much yellowish discharge and background ulcer scar was observed in the anal canal. However, cobble-stone appearance and pseudopolyposis were not present. Therefore, we clinically diagnosed him as having intestinal tuberculosis with anal fistula and prescribed antituberculosis medications. Follow-up colonoscopy performed 3 months later showed much improved multiple large irregular ulcers in the distal sigmoid colon and proximal rectum along with completely resolved anal fistula without evidence of pus discharge. (Korean J Gastroenterol 2013;62:370-374)