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白花蛇舌草 메탄올 抽出物의 抗腫瘍 效果 및 抗癌 棋戰에 關한 硏究
魯勳政,文九,文錫哉,元秦熹,文永昊,朴來佶 대한한방종양학회 2000 대한한방종양학회지 Vol.6 No.1
Objectives: This experimental study was carried out to evaluate the effects of aqueous and methanol extracts of Hedyotis diffusa which has long been used for cancer treatment in oriental medicines on the induction of apoptotic cell death in human lymphoid leukemia cell line, HL-60. Methods: Cells were treated with various concentrations (200 to 0.4㎍) and periods (6 to 30 hr) of H_2O and methanol extracts of Hedyotis diffusa. Then, cells were tested for viability by MTT assay. Cells wrere treated with 200㎍/ml of methanol extract fork various periods. Genomic DNA was isolated, separated, on 1.5% agarose gels, stained with ethidium bromide and visualized under UV light. Cells were treated with 200㎍/ml of each extract for 16hr.Then, cells were treated with various doses of each extract for 12 hr and 100㎍/ml of methanol extract for various periods. Lysate from the cells used to measure the activity of caspase-1 and-3 proteases by using fluorogenic peptide substrates including acetyl-YVAD-AMC and acetyl-DEVD-AMC, respectively, Cells were treated with 200㎍/ml of each extract for various periods. Cell lysates were immunoprecipated with anti-JNKl antibodies. The immune complex was reacted with 32^p-ATP and c-Jun as a substrate. The phosphotransferase activity of JNKI was measured by suing Phosphoimage analyzer (Fuji Co., Japan). Nuclear extracts were isolated and incubated with oligonucleotide probe of NF-kB was measured by using EMSA and visualized by PhosphoImage analyzer (Fuji Co, Japan). Cell lysates were prepared and analyzed by Western blotting with anti-Bc12 antibodies and anti-Bax antibodies. Cells were pretreated with various doses of methanol extract for 2 hr. Then, the extract was removed by centrifugation. Cells were resuspended with RPMI-1640 media containing 0.3% agarose, 10% FBS, overlayred onto bottom layer agarose and incubated at CO_2 incubator for 6 days. The number of colony was cunted under light microscopy (×100). Results: The death of HL_60 cells was markedly induced by the addition of methanol extract of Hedyotis diffusa in a dose and time-dependent manners. The apoptotic characteristic ladder pattern of DNA strand break was observed in death of HL_60 cells. In addition, it was shown nucleus chromatin condensation under Hoechst staining. Therefore, Hedyotis diffusa extract-induced death of HL-60 cells is mediated by apoptotic signaling processes. The activity of Caspaxe 3-like proteases remained in a basal level in HL-60 cells treated with aqueous extract of Hedyotis diffusa. However, it was markedly increased in HL-60 cells treated with methanol extract of Hedyotis diffusa. In addition, the phosphotransferase activity of JNKl was increased in HL-60 cells treated with methanol extract of Hedyotis diffusa. Furthermore, the activation of transcriptional activator, NF-kB was markedly induced by methanol extract of Hedyotis diffusa. Anti-apoptotic Bc12 was cleaved into 23Kda fragment by treatment of methanol extract of Hedyotis diffusa. However, expression of proapoptotic Bax protein was increased by treatment of methanol extract of Hedyotis diffusa in a time-dependent manner. Furthermore, methanol extract markedly inhibited the colony forming efficiency of HL-60 cells in semisolid agar culture. Conclusions: Above results suggest that methanol extract of Hedyotis diffusa induces the apoptotic death of human leukemic HL-60 cells via activations of Caspase-3 proteases, JNKI, transcriptional activator NF-κB, In addition, our results also suggest that methanol exthanol extract of Hedyotis diffusa reduces the malignant potential of HL-60 cells via down regulation of colony forming effciency through cleavage of Bc12 as well as induction of Bax.
Jeong, Seong-Gu,Kim, Sunggyu,Kim, Han Gyung,Kim, Eunji,Jeong, Deok,Kim, Ji Hye,Yang, Woo Seok,Oh, Junsang,Sung, Gi-Ho,Hossain, Mohammad Amjad,Lee, Jongsung,Kim, Jong-Hoon,Cho, Jae Youl Elsevier 2019 Journal of Ethnopharmacology Vol.231 No.-
<P><B>Abstract</B></P> <P><B>Ethnopharmacological relevance</B></P> <P> <I>Mycetia cauliflora</I> Reinw. (Rubiaceae) has been used as a traditional remedy to ameliorate clinical signs of inflammatory diseases, including pain, inflammation, ulcers, and wounds. Among the Mycetia subfamilies, the molecular and cellular mechanisms of <I>Mycetia longifolia</I> (Rubiaceae) have been studied. However, those of <I>Mycetia cauliflora</I> are not clearly understood. Comprehensive investigation of this plant is necessary to evaluate its potential for ethnopharmacological use.</P> <P><B>Materials</B></P> <P>and methods: The activities of <I>Mycetia cauliflora</I> methanol extract (Mc-ME) on the secretion of inflammatory mediators, the mRNA expression of proinflammatory cytokines, and identification of its molecular targets were elucidated using lipopolysaccharide (LPS)-induced macrophage-like cells. Moreover, the suppressive actions of Mc-ME were examined in an LPS-induced peritonitis mouse model.</P> <P><B>Results</B></P> <P>At nontoxic concentrations, Mc-ME downregulated the release of nitric oxide (NO), the mRNA expression of inducible nitric oxide synthase (iNOS), and the mRNA expression of interleukin (IL)-1β from LPS-activated RAW264.7 cells. This extract also inhibited the nuclear translocation of p65 and p50 and the phosphorylation of IκBα, IKK, and AKT. Western blot analysis and <I>in vitro</I> kinase assays confirmed that phosphoinositide-dependent kinase-1 (PDK1) is the direct immunopharmacological target of Mc-ME effect. In addition, Mc-ME significantly reduced inflammatory signs in an animal model of acute peritonitis. These effects were associated with decreased NO production and decreased AKT phosphorylation.</P> <P><B>Conclusion</B></P> <P>Our results suggest that Mc-ME displays anti-inflammatory actions in LPS-treated macrophage-like cells and in an animal model of acute inflammatory disease. These actions are preferentially managed by targeting PDK1 in the nuclear factor (NF)-κB signaling pathway.</P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>
Anti-stress Effect of Scutellaria baicalensis in SD Rats and ICR Mice
( Jong Hoon Ryu ),( Blendyl Saguan Tan Lee ),( Ji Wook Jung ),( Nam Yoon Ahn ),( Seung Joo Lee ),( Gu Young Yu ),( Shin Ha Han ),( Jeong Hoon Lee ),( Geum Seon Lee ),( Jae Hoon Cheong ) 한국응용약물학회 2004 Biomolecules & Therapeutics(구 응용약물학회지) Vol.12 No.1
N/A Pyroligneous liquid(PL) is produced by carbonizing Oak in 350∼400℃. It is traditionally used for treating stress-related disorder, hepatic disease, immune disorder, G-I disorder and inflammatory disease. The aim of this study is to investigate anti-stress effects of PL. The experiments were performed with the use of young(9 weeks of age) male rats of SD strain and the male ICR mice (20-25g). Animals of the normal group were not exposed to any stress and the control group were exposed to stress. The rats of the Ginseng, diazepam(BZ) and PL supplementary group were orally administered once a day 100g of Ginseng extract-kg body weight, 5 mg of BZ/kg body weight and 1 ml of PL/100 g body weight and then exposed to stress. The mice of the Ginseng, BZ and PL supplementary group were given water containing 100 g of Ginseng extract/100 ml potable water, 5 mg of BZ/kg 100 ml of drinking water and 10 ml of PL/100 ml of drinking water and exposed to stress. Animals were given materials for 7 days after stabilizing them, and then were given supplementary materials for 5 days with stress. They were stressed by immobilization for 30 minutes and then the animals were exposed to electroshocks for 5 minutes. We recorded stress-related behavioral changes of experimental animals by stressing them using the Etho-vision system and measured the levels of corticosterone in blood While stress suppressed locomotor activity of animals, PL-supplementation partially blocked the stress effect of locomotion in rats and mice, and also partially blocked stress-induced behavioral changes such as freezing, burrowing, smelling and rearing activity in rats and in closed area it increased relatively in elevated plus maze test. However, these changes also partially were blocked by PL-supplementation. PL-supplementation decreased levels of blood corticosterone increased by stress in rats. These results suggest that PL protects partially the living organism from stress attack in some cases.
정훈구(Jeong, Hoon Gu),반영환(Pan, Young Hwan) 한국디지털디자인협의회 2015 디지털디자인학연구 Vol.15 No.3
SNS는 이미 많은 스마트폰 사용자들의 생활 속에 소통의 도구이자, 무료함을 달래줄 도구로 자리 잡았지만 SNS의 사용자 인터페이스에 관련한 연구는 많지 않으며 여러 SNS에서 채택하고 있는 카드형 UI에 대한 선행연구 또한 아직 진행된 바 없다. 현재 사용자들에게 노출되는 카드형 UI가 인터페이스 측면에서 어떠한 문제점이 있고, 이에 따라 낮은 사용성을 야기하는지를 알아볼 필요가 있다. 따라서 본 논문에서는 기존 SNS의 카드형 UI에 대한 비교 분석을 진행하고, 가설을 설정하여 가설검정을 시행하였다. 연구의 목적은 최적의 카드형 UI를 구성하기 위해서는 어떠한 시각요소를 통해 디자인되어야 하는지 파악하고, 파악된 시각요소가 유효할 지 검증하기 위함이다. 본 연구는 위와 같은 목적에 따라 비교분석과 문헌연구, 가설의 검증을 병행하여 진행하였다. 1차로 기존의 SNS 비교분석을 통해 몇 가지 SNS의 카드형 UI 형태를 파악하였다. 2차로 GUI와 관련한 문헌과 선행연구를 통해 효과적인 정보 전달과 편의성을 위해서는 어떠한 시각요소가 고려되어야 할지 분석하였다. 3차로는 고려되어야 할 시각요소를 바탕으로 세 가지 가설을 설정하고 이에 따라 프로토타입을 제작하여 웹 설문조사를 시행하였다. 본 연구는 향후 카드형 UI가 적용될 새로운 앱의 인터페이스 연구 자료로 활용할 수 있을 것으로 기대한다. SNS has already established itself as a tool of lot of smart phones users to communicate in their life as well as to relieve their oredom. However, studies related to the user interface of the SNS are not many, and previous studies on the card-type UI adopted in various SNS. It is necessary to find out what kind of problems the card-type UI that is exposed to the current users in terms of interface has and accordingly causes low usability. In this paper, therefore, a comparative analysis has been performed of the conventional card-type UI of the SNS, and hypothesis setting and testing were conducted. The purpose of the study is to identify with what kind of visual elements the design should be done to configure optimal card-type UI and to verify whether the identified visual elements are valid. This study was performed in accordance with these purposes with comparative analysis, literature study and hypothesis verification. First of all, some forms of the card-type UI of SNS were identified through comparison analysis of conventional SNS. Second, literature and previous studies related to the GUI were performed to analyze which visual elements should be considered for effective information transfer and convenience. Third, three hypotheses were set on the basis of visual elements to be considered, and web surveys were implemented accordingly after making the prototype. This study can be expected to be useful as an interface reference for new apps that will apply to card-type UI in the future.
Kim, Jeong-Ah,Kim, Won-keun,No, Jin Sun,Lee, Seung-Ho,Lee, Sook-Young,Kim, Ji Hye,Kho, Jeong Hoon,Lee, Daesang,Song, Dong Hyun,Gu, Se Hun,Jeong, Seong Tae,Park, Man-Seong,Kim, Heung-Chul,Klein, Terry Public Library of Science 2016 PLoS neglected tropical diseases Vol.10 No.6
<▼1><P><B>Background</B></P><P>Hantaan virus (HTNV), a negative sense tripartite RNA virus of the Family <I>Bunyaviridae</I>, is the most prevalent hantavirus in the Republic of Korea (ROK). It is the causative agent of Hemorrhagic Fever with Renal Syndrome (HFRS) in humans and maintained in the striped field mouse, <I>Apodemus agrarius</I>, the primary zoonotic host. Clinical HFRS cases have been reported commonly in HFRS-endemic areas of Gyeonggi province. Recently, the death of a member of the ROK military from Gangwon province due to HFRS prompted an investigation of the epidemiology and distribution of hantaviruses in Gangwon and Gyeonggi provinces that border the demilitarized zone separating North and South Korea.</P><P><B>Methodology and Principal Findings</B></P><P>To elucidate the geographic distribution and molecular diversity of HTNV, whole genome sequences of HTNV Large (L), Medium (M), and Small (S) segments were acquired from lung tissues of <I>A</I>. <I>agrarius</I> captured from 2003–2014. Consistent with the clinical incidence of HFRS established by the Korea Centers for Disease Control & Prevention (KCDC), the prevalence of HTNV in naturally infected mice in Gangwon province was lower than for Gyeonggi province. Whole genomic sequences of 34 HTNV strains were identified and a phylogenetic analysis showed geographic diversity of the virus in the limited areas. Reassortment analysis first suggested an occurrence of genetic exchange of HTNV genomes in nature, ROK.</P><P><B>Conclusion/Significance</B></P><P>This study is the first report to demonstrate the molecular prevalence of HTNV in Gangwon province. Whole genome sequencing of HTNV showed well-supported geographic lineages and the molecular diversity in the northern region of ROK due to a natural reassortment of HTNV genomes. These observations contribute to a better understanding of the genetic diversity and molecular evolution of hantaviruses. Also, the full-length of HTNV tripartite genomes will provide a database for phylogeographic analysis of spatial and temporal outbreaks of hantavirus infection.</P></▼1><▼2><P><B>Author Summary</B></P><P>Hemorrhagic Fever with Renal Syndrome (HFRS) and Hantavirus Pulmonary Syndrome (HPS) are endemic zoonotic infectious diseases caused by hantaviruses that belong to the Family <I>Bunyaviridae</I> containing negative-sense tripartite RNA genomes. Hantaviruses pose a critical emerging public health threat, with up to 200,000 clinical cases reported annually worldwide with 1–36% case fatality rates. In humans, hantavirus-borne diseases are contracted by the inhalation of viruses aerosolized from rodent excreta. However, there is no effective therapeutic or vaccine to prevent from the disease. Whole genome sequences of Hantaan virus (HTNV) were acquired from lung tissues of <I>Apodemus agrarius</I> captured in HFRS-endemic areas of the Republic of Korea (ROK). Phylogenetic analyses demonstrated that sequences of the HTNV tripartite genomes clustered geographically, showing broad diversity of HTNV throughout the areas surveyed. Reassortment analysis first suggested a natural occurrence of the HTNV genetic exchange in the ROK. These observations contribute to a better understanding of the genetic diversity and molecular evolution of hantaviruses in HFRS-endemic regions. The complete sequences of HTNV genomes will provide a database for the phylogeographic analysis and surveillance of endemic hantavirus-borne diseases.</P></▼2>
박정훈(Jeong-hoon Park),김우택(Woo-taek Kim),민병진(Byeong-jin Min),이원학(Won-hak Lee),이정구(Jeong-gu Lee) 대한구강악안면외과학회 2002 대한구강악안면외과학회지 Vol.28 No.6
Treatments for restoring the function and esthetics of missing teeth include fixed bridge, partial denture, orthodontic movement, implantation and autotransplantation. However, there is no absolute indications for each techniques. Due to undevelopment of root and root resorption after autotransplantation, the success rate of autotransplantation over the past decades have been low. Recently. with the study on biological principles of the healing of periodontal ligament, the success rate of autotransplantation began to improve. We report the cases of successful autotransplantation which resulted in ideal healing of periodontal ligament, gingiva and alveolar bone.