PGA2 induces the expression of HO-1 by activating p53 in HCT116 cells

Hyein Lee,Sang-Sun Lee,Ji-Young Park,Yun-Jeong Choe,이선영,Ho-Shik Kim,H.-S. Kim 대한독성 유전단백체 학회 2017 Molecular & cellular toxicology Vol.13 No.2

Prostaglandin (PG) A2 which is a cytotoxic PG, was reported to induce the expression of heme oxygenase (HO)-1 via activation of p38MAPK to keep U2OS cells from cell cycle arrest in G2M phase. The expression of HO-1 is primarily regulated at the level of transcription. But the transcription factors that are responsible for PGA2-induced HO-1 expression were not clarified yet. Here, we report that PGA2-induced transcription of HO-1 is mediated by p53, a tumor suppressive transcription factor. In HCT116 cells, PGA2 treatment led to the phosphorylation of p53 and an increase of p21WAF1 transcription as well as the activation of HO-1 transcription. Knocking p53 down via RNA interference or inhibiting the p53’s transcriptional activity by pifithrin-α treatment led to suppression of the increase in the level of both HO-1 expression and activity of HO-1 promoter. Pretreatment of NU- 7441, a chemical inhibitor of DNA-activated protein kinase (DNA-PK), prevented both the PGA2-induced phosphorylation of p53 and an increase of HO-1 transcription. In addition, N-acetyl-l-cysteine, a scavenger of reactive oxygen species (ROS), also mimicked the effect of NU-7441 on the PGA2-induced activation of p53 and HO-1 transcription. Collectively, these results suggest that PGA2 induces the expression of HO-1 via activation of p53, which is mediated by the ROSDNA- PK pathway.

Ho-166 부착풍선도자를 이용한 방사선 조사의 돼지 관상동맥 스텐트 재협착 예방 효과

김원 ( Kim Won ),정명호 ( Jeong Myeong Ho ),박옥영 ( Park Og Yeong ),정우곤 ( Jeong U Gon ),박우석 ( Park U Seog ),김주한 ( Kim Ju Han ),안영근 ( An Yeong Geun ),조정관 ( Jo Jeong Gwan ),박종춘 ( Park Jong Chun ),강정채 ( Kang Je 한국지질동맥경화학회 ( 구 한국지질학회 ) 2002 韓國脂質學會誌 Vol.12 No.1

배경 : 국내에서 개발된 방사선 동위원소 Holmium-166 (166Ho)은 주로 베타선을 방출하며, 166Ho을 부착한 풍선도자를 이용하여 돼지 관상동맥 재협착 모형에서 풍선확장술 후 신생내막 증식을 전신적 부작용 없이 안전하고 효과적으로 억제하였음을 보고한 바 있다. 본 연구에서는 돼지 관상동맥 스텐트 재협착 모형에서 스텐트 시술 후 신생내막 증식에 의한 재협착 병변을 166Ho 부착 풍선도자를 이용하여 치료하여 그 효과를 관찰하고자 하였다. 방법

Up-regulation of Heme Oxygenase-1 by Korean Red Ginseng Water Extract as a Cytoprotective Effect in Human Endothelial Cells

Yang, Ha-Na,Lee, Seung-Eun,Jeong, Seong-Il,Park, Cheung-Seog,Jin, Young-Ho,Park, Yong-Seek The Korean Society of Ginseng 2011 Journal of Ginseng Research Vol.35 No.3

Korean red ginseng (KRG) is used worldwide as a popular traditional herbal medicine. KRG has shown beneficial effects on cardiovascular diseases, such as atherosclerosis, diabetes, and hypertension. Up-regulation of a cytoprotective protein, heme oxygenase (HO)-1, is considered to augment the cellular defense against various agents that may induce cytotoxic injury. In the present study, we demonstrate that KRG water extract induces HO-1 expression in human umbilical vein endothelial cells (HUVECs) and possible involvement of the anti-oxidant transcription factor nuclear factor-eythroid 2-related factor 2 (Nrf2). KRG-induced HO-1 expression was examined by western blots, reverse transcriptase polymerase chain reaction and immunofluorescence staining. Specific silencing of Nrf2 genes with Nrf2-siRNA in HUVECs abolished HO-1 expression. In addition, the HO inhibitor zinc protoporphyrin blunted the preventive effect of KRG on $H_2O_2$-induced cell death, as demonstrated by terminal transferase dUTP nick end labeling assay. Taken together, these results suggest that KRG may exert a vasculoprotective effect through Nrf2-mediated HO-1 induction in human endothelial cell by inhibition of cell death.

Up-regulation of Heme Oxygenase-1 by Korean Red Ginseng Water Extract as a Cytoprotective Effect in Human Endothelial Cells

Hana Yang,Seung Eun Lee,Seong Il Jeong,Cheung-Seog Park,Young-Ho Jin,Yong Seek Park 고려인삼학회 2011 Journal of Ginseng Research Vol.35 No.3

Korean red ginseng (KRG) is used worldwide as a popular traditional herbal medicine. KRG has shown beneficial effects on cardiovascular diseases, such as atherosclerosis, diabetes, and hypertension. Up-regulation of a cytoprotective protein, heme oxygenase (HO)-1, is considered to augment the cellular defense against various agents that may induce cytotoxic injury. In the present study, we demonstrate that KRG water extract induces HO-1 expression in human umbilical vein endothelial cells (HUVECs) and possible involvement of the anti-oxidant transcription factor nuclear factor-eythroid 2-related factor 2 (Nrf2). KRG-induced HO-1 expression was examined by western blots, reverse transcriptase polymerase chain reaction and immunofluorescence staining. Specific silencing of Nrf2 genes with Nrf2-siRNA in HUVECs abolished HO-1 expression. In addition, the HO inhibitor zinc protoporphyrin blunted the preventive effect of KRG on H₂O₂-induced cell death, as demonstrated by terminal transferase dUTP nick end labeling assay. Taken together, these results suggest that KRG may exert a vasculoprotective effect through Nrf2-mediated HO-1 induction in human endothelial cell by inhibition of cell death.

개 아토피성 피부염 장기치료 1례

박은정,윤기영,박정호,이왕희,박성준 忠南大學校 獸醫科大學 附設 動物醫科學硏究所 2011 動物醫科學硏究誌 Vol.18 No.1

A 7-year-old male castrated Shih-tzu dog was referred to the Veterinary Medical Teaching Hospital of Chungnam National University for severe pruritus. On physical examination were confirmed systemic erythema and alopecia, crust on the neck and flank region. Tape strip test identified numerous degenerative neutrophils and severe Malassezia infection. Treatment for severe pruritus was applied with systemic antihistamine therapy and topical therapy with antimicrobial shampoos. For the finding of appropriate treatment of atopic dermatitis, it was chosen six immune-related treatments. And clinical sign scores were evaluated by investigator with CADESI-03 after each application of six treatments. Application of human immunoglobulin G and leflunomide showed the remarkable regression of lesions.

방사선사진의 인덱스-순서 분석을 통한 치아경조직의 탈염화 검출

박동현,박영호,김경숙,박정훈,이기자,최삼진,최용석,황의환,박헌국 대한구강악안면방사선학회 2009 Imaging Science in Dentistry Vol.39 No.2

Purpose : The purpose of this study was to develop the radiographic technique for detecting the demineralization which is known as indication of dental caries. Materials and Methods : This technique was based on the comparing of multiple radiographs which was irradiated by multiple X-ray spectra. For the meaningful comparing, the multiple radiographs were reconstructed to the dosimetrically consistent images using a standard material. The difference of resulting images of same target with multiple spectra represents the difference of response of material as regards the spectra. Results : We have found about 10% of demineralization of dental hard tissues particularly in the proximal region through the analyzing of differences. Conclusion : Most intriguing thing in this investigation was that the method to analyze difference shows us to an anatomic structure of dental hard tissues even if absolute values of optical density were excluded during the procedures.

Benefits and problems in implementation for integrated medical Information system

Park, Chang-Seo,Kim, Kee-Deog,Park, Hyok,Jeong, Ho-Gul 대한구강악안면방사선학회 2005 Imaging Science in Dentistry Vol.35 No.4

Purpose : Once the decision has been made to adopt an integrated medical information system (IMIS), there are a number of issues to overcome. Users need to be aware of the impact the change will make on end users and be prepared to address issues that arise before they become problems. The purpose of this study is to investigate the benefits and unexpected problems encountered in the implementation of IMIS and to determine a useful framework for IMIS. Materials and Methods : The Yonsei University Dental Hospital is steadily constructing an IMIS. The vendor's PACS software, Piview STAR, supports transactions between workstations that are approved to integrating the healthcare enterprise (IHE) with security function. It is necessary to develop an excellent framework that is good for the patient, healthcare provider and information system vendors, in an expert, efficient, and cost-effective manner. Results : The problems encountered with IMIS implementation were high initial investments, delay of EMR enforcement, underdevelopment of digital radiographic appliances and software and insufficient educational training for users. Conclusions : The clinical environments of dental IMIS is some different from the medical situation. The best way to overcome these differences is to establish a gold standard of dental IMIS integration, which estimates the cost payback. The IHE and its technical framework are good for the patient, the healthcare provider and all information systems vendors.

3차원 전산화단층촬영 영상을 이용한 안면 연조직 두께 계측의 임상적 유용성

정호걸,김기덕,한승호,허경석,이제범,박혁,최성호,김종관,박창서 대한구강악안면방사선학회 2006 Imaging Science in Dentistry Vol.36 No.2

Purpose : To evaluate clinical usefulness of facial soft tissue thickness measurement using 3D computed tomographic images. Materials and Methods : One cadaver that had sound facial soft tissues was chosen for the study. The cadaver was scanned with a Helical CT under following scanning protocols about slice thickness and table speed; 3 mm and 3 mm/sec, 5 mm and 5 mm/sec, 7 mm and 7 mm/sec. The acquired data were reconstructed 1.5, 2.5, 3.5 mm reconstruction interval respectively and the images were transferred to a personal computer. Using a program developed to measure facial soft tissue thickness in 3D image, the facial soft tissue thickness was measured. After the ten-time repeation of the measurement for ten times, repeated measure analysis of variance (ANOVA) was adopted to compare and analyze the measurements using the three scanning protocols. Comparison according to the areas was analyzed by Mann-Whitney test. Results : There were no statistically significant intraobserver differences in the measurements of the facial soft tissue thickness using the three scanning protocols (p>0.05). There were no statistically significant differences between measurements in the 3 mm slice thickness and those in the 5 mm, 7 mm slice thickness (p>0.05). There were statistical differences in the 14 of the total 30 measured points in the 5 mm slice thickness and 22 in the 7mm slice thickness. Conclusion : The facial soft tissue thickness measurement using 3D images of 7 mm slice thickness is acceptable clinically, but those of 5 mm slice thickness is recommended for the more accurate measurement.

광조사 방식이 복합레진의 중합과 누출에 미치는 영향

박종진,박정원,박성호,박주명,권태경,김성교 大韓齒科保存學會 2002 Restorative Dentistry & Endodontics Vol.27 No.2

The aim of this study was to investigate the effect of light irradiation modes on polymerization shrinkage, degree of cure and microleakage of a composite resin. VIP^™ (Bisco Dental Products, Schaumburg, IL, USA) and Optilux 501^™(demetron/ Kerr, Danbury, CT, USA) were used for curing Filtek^™ Z-250(3M Dental Products, St. Paul., MN, USA) composite resin using following irradiation modes: VIP^™ (Bisco) 200mW/cm^2 (V2), 400mW/cm^2 (V4), 600mW/cm^2 (V6), Pulse-delay (200 mW/cm^2 3 seconds, 5 minutes wait, 600mW/cm^2 30seconds, VPD) and Optilux 501^™ (Demetron/Kerr) C-mode (OC), R-mode (OR). Linear polymerization shrinkage of the composite specimens were measured using Linometer (R&B, Daejeon, Korea) for 90 seconds for V2, V4, V6, OC, OR groups and for up to 363 seconds for VPD group (n=10, each). Degree of conversion was measured using FTIR spectrometer (IFS 120 HR, Bruker Karlsruhe, Germany) at the bottom surface of 2 mm thick composite specimens. V2, V4, V6, OC groups were measured separately at five irradiation times (5, 10, 20, 40, 60 seconds) and Or, VPD groups were measured in the above mentioned irradiation modes (n=5, each). Microhardness was measured using Digital microhardness tester (FM7, Future-Tech Co., Tokyo, Japan) at the top and bottom surfaces of 2mm thick composite specimens after exposure to the same irradiation modes as the test of degree of conversion(n=3, each). For the microleakage test, class V cavities were prepared on the distal surface of the ninety extracted human third molars. The cavities were restored with one of the following irradiation modes: V2/60 seconds. V4/40 seconds, V6/30 seconds, VPD, OC and OR. Microleakage was asessed by dye penetration along enamel and dentin marigns of cavities. Mean polymerization shrinkage, mean degree of conversion and mean microhardness values for all groups at each time were analyzed using one-way ANOVA and Duncan's multiple range test, and using chisquare test for microleakage values. The results were as follows: ·Polymerization shrinkage was increased with higher light intensity in groups using VIP^™(Bisco) : the highset with 600mW/cm^2, followed by Pulse-delay, 400mW/cm^2 and 200mW/cm^2 groups. The degree of polymerization shrinkage was higher with Continuous mode than with Ramp mode in groups using Optilux 501^™ (Demetron/Kerr). ·Degree of conversion and microhardness values were higher with higher light intensity. The final degree of conversion was in the range of 44.7 to 54.98% and the final microhardness value in the range of 34.10 to 56.340. ·Microleakage was greater in dentin margin than in enamel margin. Higher light intensity showed more microleakage in dentin margin in groups using VIP^™ (Bisco). The microleakage was the lowest with Continuous mode in enamel margin and with Ramp mode in dentin margin when Optilux 501^™ (Demetron/Kerr) was used.

설악산 국립공원의 어류분포

장민호,조가익,하진용,정광석,박성배,주기재 한국어류학회 2002 韓國魚類學會誌 Vol.14 No.4

설악산 국립공원지역 산간계류의 17개 지점에서 2000년 6월부터 2001년 6월까지 어류상 및 이화학적 요인을 계절별로 총 4회에 걸쳐 분석하였다. 모든 조사지점은 1~3차의 하천으로 비교적 물리·화학적 교란이 적었으며 하천폭은 5~30 m 정도였다. 채집된 어류는 총 18과 42종 2,557개체였고, Cyprinidae는 16종으로 84.7%의 상대풍부도를 나타냈으며, Gobiidae 4종, 5.0?의 상대풍부도를 보였다. Cyprinidae는 전체 채입종의 37.2%를 나타냈다. 우점종은 Zacco temminck로 10개 지점에서 738개체가 채집되어 28.9%의 상대풍부도를 나타냈으며, 아우점종은 Rhynchocypris kumkangensis와 Z. platypus로 각각 4개 지점 648개체(25.3%)와 7개 지점 344개체(13.5%)로 나타났다. 한국고유종(Korean endemic species)은 모두 4과 11종 781개체로 30.5%의 상대풍부도를 나타냈으며, 고유화 빈도는 25.6%로 한국의 평균 고유화 빈도와 유사하게 나타났다. 본 조사에서 외래어는 채집되지 않았다. 국립공원 내에 서식하는 어류의 다양성 유지와 회복을 위해서는 공원 내는 물론 경계지역 하천의 체계적인 관리가 요구된다. The fish fauna of mountain streams in the Seorak National Park area was investigated from June 2000 to June 2001 (4 times). All 17 sites were relatively undisturbed 1st to 3rd order streams. A total of 2,557 individuals were collected and classified into 17 families and 43 species. Cyprinidae accounted for 37.2% (16 species) of the total fish species and 84.7% (2,165 ind.) of the total abundance. Subdominant families were Gobiidae (4 species, 5.0%) and Balitoridae (3 species, 3.4%). Zacco temmincki (RA 28.9%, 10 sites) dominated. Subdominant species were Rhynchocypris kumkangensis (25.3%, 4 sites) and Zacco platypus (13.5%, 7 sites). Eleven of the species are endemic to Korea (4 families, 30.5%). Exotic species were not collected in this survey. Fish diversity was higher in streams around the boundary area than in the national park. Therefore, management of streams in the boundary area to protect the fish biodiversity can be efficiently conducted. Habitat degradation should be reduced for the conservation and restoration of fish diversity.