서울의 Penicillinase Producing Neisseria gonorrhoeae 발생빈도(1998)

김재홍,김준호,반재용,이정우,황성주,정준규,정성태,강진문,조흔정,홍창의,정혜신,이한승,김이선,이봉길,이종호,선영우,한기덕,윤성필,이성훈,안종성,박석범,문승현,조항래,김형섭,류지호,황재영,박준홍,손상욱 한양대학교 의과대학 2001 한양의대 학술지 Vol.21 No.1

In recent years, gonorrhea has been pandemic and remains one of the most common STDs in the world, especially in developing countries. For the detection of a more effective therapeutic regimen and assessing the prevalence of Penicillinase Producing Neisseria gonorrhoeae(PPNG), we have been trying to study the patients who have visited the Venereal Disease Clinic of Choong-Ku Public Health Center in Seoul since 1980 by menas of the chromogenic cephalosporin method. In 1998, 93 strians of N. genorrhoeae were isolated, among which 60(64.5%) were PPNG. The prevalence of PPNG in Seoul, which had been decreased to 39% in 1996 after a peak of 74.3% in 1993, is increased to 64.5% in 1998.

갑상선기능저하증에 기인한 내인성 급사 1예 보고

이정규,전지현,김민정,이규재,김한겸,조현득,채양석 大韓法醫學會 1999 대한법의학회지 Vol.23 No.2

Although it is known that hypothyroidism can cause cardiac dysfunction, it is very hard to find a case report on sudden death due to hypothyroidism. There are only two reported cases on mediline; one is about a 15-year-old girl who died suddenly of Hashimoto's thyroiditis and the other is about a 31-year-old man who also died suddenly of hypothyroidism associated with chronic lymphocytic thyroiditis. Recently we found a young woman who unexpectedly died while she was sleeping. She was diagnosed as hypothyroidism when she went to hospital about a month before she died. At autopsy there were roughly four abnormal findings; Hashimoto's thyroiditis, mild chronic active hepatitis, diverticulosis and accessory spleen. Trace of chlorpheniramine was detected in gastric remains and blood alcohol level was 0.37 percent. To our knowledge, this is the first case reporting sudden and unexpected natural death associated with hypothyroidism in Korea.

흰쥐에서의 카르복시메칠셀룰로오스나트륨과 겔란검 혼합겔의 유착방지 효과

권윤환,이시범,이정권,박용덕,박정숙,황성주 한국약제학회 2004 Journal of Pharmaceutical Investigation Vol.34 No.6

The aim of the present study was to investigate the efficacy of a mixed gel formulation composed of sodium carboxymethyl cellulose and gellan gum (Na-CMC gel) for the prevention of adhesions after laminectomv. The anti-adhesive effect of the Na-CMC gel was tested in a controlled randomized study using an animal model of lumbar laminectomv. The animals (60 female Sprague-Dawlev rats) were randomly allocated into two treatment groups to receive the Na-CMC gel on the injured area or no gel (control). The incidence of adhesions and their grade were blindly evaluated at 4. 8 and 12 weeks after surgery. The amount of scar tissue and tenacity were grossly reduced by the Na-CMC gel at postoperative 4. 8. and 12 weeks. The mean adhesion scores were 0.75. 125. and 1.38 at 4. 8. and 12 weeks in the gel-treated group. respectively. No significant inflammatory reaction was observed and the healing of wound was not affected by the Na-CMC gel. The Na-CMC gel reduced the amount of scar formation and tenacity in rat laminectomv model without affecting the healing of operation wound and other complications. Therefore. the Na-CMC gel may be the potential to prevent postsurgical adhesions in clinical state.

감자식초의 항돌연변이원성 및 세포독성 효과

함승시,김미남,정용진,이득식 동아시아식생활학회 2001 동아시아식생활학회지 Vol.11 No.1

This study was performed to examine the antimutagenic and cytotoxic effects of potato vinegar and commercial vinegars(cider, brown rice, persimmon vinegars) on Salmonella typhimurium TA98. TA100 and cancer cell lines using Ames test and cytotoxicity assay, respectively. In Ames test, all vinegars did not exhibit any mutagenicity, but showed substantial inhibitory effects against N-methyl-N-nitro-N-nitrosog-uanidine(MNNG), 4-nitroquinoline-1-oxide(4NQO), 3-amino-1.4-dimethyl-5H-pyrido[4,3-b] indol(Trp-P-1) and benzo(a)pyrene(B(a)P). The number of revertants per plate decreased significantly when these vinegars(80㎍/plate) were added to the assay system using TA100 strain. Especially, potato vinegar(80㎍/plate) showed high inhibition rate of 69.9% against mutagenicity of B(a)P on TA100 strain. In the cytotoxicity assay, these vinegars also showed prominent cytotoxic activity against human cancer cell lines. Potato vinegar(10㎍/showed the strongest cytotoxic effect against HT1080 (fibrosacoma cell) and K562 (myelogenous leukemia) at the same concentration when compared with other vinegars.

Gas-Liquid Chromatography를 이용한 사과 및 배 중의 농약 다성분 잔류분석법

박주황,김택겸,오창환,김정한,이영득,김장억 한국환경농학회 2004 한국환경농학회지 Vol.23 No.3

사과 및 배 시료에서 농약 다성분의 gas chromatography를 이용한 잔류분석법 확립을 위하여 199가지의 농약를 선정 하여 retention time 및 검출기에 따라 ECD 5 그룹 및 NPD 5 그룹의 10개 그룹으로 분류하였다. 시료의 종류에 따른 분석조건을 확립하기 위한 회수율 시험은 농약들의 log P_(ow) 값과 화학적 분류에 따라 총 18개 (ECD 11개, NPD 7개)의 농약을 선정하였다. 예비실험 후 확립된 분석방법에 따라 10개 그룹의 혼합 표준용액으로 사과 및 배에 대한 회수율시험을 행하였다. 그 결과, 총 196가지의 농약의 70%에 해당하는 사과에서 136개, 배에서 133개의 농약들에서 회수율 70에서 120%의 양호한 결과를 나타내었다. 그러나 사과에서 43개, 배에서 45개의 농약들이 70% 미만의 회수율을 보였고, fenvalerate는 120% 이상의 회수율을 나타내었으며, 사과에서 17개 및 배에서 18개의 농약들은 검출이 되지 않았다. 그러나 확립된 분석법은 SOP에 의한 신속하고 수월한 수행으로 농산물 중의 잔류농약을 검출 및 모니터링하는 목적에 적합하다고 사료된다. A rapid analytical method was developed to determine multiple pesticide residues in apples and pears using gas-liquid chromatography (GLC). The samples were extracted with water-miscible solvents and purified by cleanup procedures serially comprising liquid-liquid partition and solid-phase extraction (SPE). Each analyte was separated and determined by a high-resolution GLC equipped with electron-capture detector (ECD) and nitrogen-phosphorous detector (NPD). A total of 196 pesticides, which were previously classified into 5 groups each for ECD and NPD based on their retention behaviors on the capillary column and responses to the detector, were subjected to the recovery experiment. In compliance with the analytical criteria, 70 to 120% of recovery and less than 20% relative standard deviation, the proposed method could be successfully applied to analyze 136 and 133 pesticide residues in apples and pears, respectively, which enabled not only rapid screening but quantitation of the residues. Even though less reliability was resulted from unacceptable recovery range, rest of pesticides including 43 and 45 analytes in apples and pears, could be also detected for their identity. The proposed method, failed to cover 17 and 18 pesticides for apples and pears, which mostly showed high polarity or heat-lability but, could be suitable for fast surveilance or monitoring of fruit harvests.

마우스 종양발생에서 Nitric Oxide의 역할에 관한 연구 Ⅲ : Helicobacter pylori에 의해 유발된 마우스 위암에서 염증매개인자의 역할 The role of inducible nitric oxide synthase and cyclooxygenase-2 in H. Pylori-associated gastric carcinogenesis

남기택,오상연,조현무,이국경,강진석,제정환,최미나,한상욱,김대용,장동덕,양기화,안병우 식품의약품안전청 2001 식품의약품안전청 연보 Vol.5 No.-

feficotorfer fyf☞ri(Hp)가 위암파 관련이 있다는 역학적인 증거는 많이 있지만 이에 대한 정확한 기전에 대해선 밝혀져 있지 않고 있으며 실험동물 모델도 적절하지 못한 것으로 알려져있다. 본 실험에서는 위암의 원인으로 알려져 있는 f, fyforf'(Hp)를 이용하여 마우스에서 위암 모델을 확립하고 만성염증과정 중에 생성되는 리0와 COX-2 등의 발현이 위암발생에 미치는 명향을 통하여 예방과 치료를 위한 점근을 시도하고자 하였다. 마우스를 7군으로 나누어서 1, 2, 3, 4군의 등물은 MNU를 증류수에 200ppm 농도로 음수병득 이용하띤 10주간 격주로 투여하였으며 MHU 음술 투여 1주 휴씩 후 배양한 f. fyrofi 를 약 109cru/rfll 로 맞춰 한 마리당 0.1ml 씩 이틀 간격으로 세 번에 걸쳐 하룻방 금식시킨 1, 2, 3, 5, 6, 7군기 마우스의 위장에 투입하떴다. 균 투입을 마친 후 다응 날부터 2군쏙 6군은 iNOS 억제제인 aminoguanidine(AG)을 음수병으로 툰여하였으며 3군과 7군은 COX-2 척제제인 nimesulide(NSD)를 투여하였다. 위의 종양발생양상을 샅최보면 bfNU와 Hp만을 투여한 1 관 ; (hfNU +Hp), 2군 : iNO을 inhibitor 투여군(MNU+HP+AG'1. 3군 ;CO딘-2 Inilibitor 투여군(MNU누Hp누 NSD), 4군 ,MNlf 단독투여군, 5군 ;Hp 단독투여춘, 6군 ; 러p 단독에 AG투여군, 7군 , Hp 단독에 NSD투여군의 종양발샐을은 각각 쁜.Bff(l1/16), 70.6%f12/ti), 했.9ff(7/18), 10%(1/10), Off(O/IS)0%(O/S), 0%(O/5)의 발쟁율을 보여 iNOS 억제제인 AC은 좁양발생을 억계하지 못하였으며, COX-2억제제인 NSD 는 종양발생을 유의적으로 감소시켰다. 콩양발생개수에서는 2.62±0.36, 1.41츠0.14, 0.44 르0.12, 0.10±0.10을 보여 AC와 NSD에서 유의성 있게 발생개수를 줄였다. Hp 단독투여에 의해즌는 종양발생이 나타나지 않았으며 HP+AG, Hp+ IfSD 추여군에콕 시험증료 시점에 약물에 의해 Hp의 제균효과가 있는지의 여부를 확인끓기 위하여 PCR을 이용하여 확인한 결과 모두 양성인 것으로 나타나 Hp의 제균효과에 의한 촐양발생 억제가 일어나지는 않았다. 위의 결과로 볼 때 Hp는 위암발생을 촉진하는 것으로 나타났고 딘p 감염시 매개되는 염증인자를 억제하였을 때 종양발생을 억제하는 것으로 위암 발생에서 염증매개인자는 종양을 촉진하는 것으로 나타났으며 it,705 억제제쓱 COX-』 억제제의 위알 예밭효과fl는 효과적일 것으로 사료된다 In spite of a large volume of epidemiological evidence indicating significant relationship between H. pylori infection and gastric adenocarcinoma, a doubt still exists on an elevated risk of stomach cancer by H. pylori infection due to lack of direct evidence of their exact mechanistic link. It is, therefore, essential to have an appropriate animal model for detailed analysis of the role of H. pylori played in gastric carcinogenesis. There is a wealth of evidence to support that over production of inducible nitric oxide synthase(iNOS) and cyclooxygenase-2(COX-2) is involved in the pathogensis of various cancer in both rodents and humans. The aim of this study was to establish a mouse model for H. pylori-associated gastric carcinogenesis and to identify the role of inducible nitric oxide synthase(iNOS) and cyclooxygenase-2(COX-2) played during the gastric carcinogenesis in mice. Eighty-three specific pathogen free, six-week-old male C57BL/6 mice were randomly divided into seven groups. Animals of the group 1, 2, 3, 4 were given MNU in their drinking water at the concentration of 200 p.p.m. for total five cycles of one-week regimen with one-week pause. After completion of MNU administration, they were given autoclaved distilled water for one weeks, and groups 1, 2, 3, 5, 6, 7 were inoculated with H. pylori. After completion of H. pylori. inoculation, groups 2 and 6 were given aminoguanidine in their drinking water at concentration of 1000p.p.m. and animals of group 3 and 7 were given the diet containing 200 ppm nimesulide at 12 weeks of age. All animals were killed at 50 weeks of age. The incidences of the glandular stomach tumors in the group 1, 2, 3 and 4 were 87.5%(14/16), 76.4%(13/17), 44.4(8/18), 10.0%(1/10), respectively and the tumor incidence of group 3(MNU→Hp+nimesulide) was significantly lower than those of group 1(MNU→Hp) at the value of P<0.01. The average numbers of tumors of group 2(MNU→Hp+AG : 1.41±0.24) and group 3(MNU→Hp+nimesulide : 0.44±0.12) were significantly lower than those of group 1(MNU→Hp : 2.62±0.36) at the value of P<0.05. Therefore, overproduced iNOS and COX-2 plays an important role in mice gastric carcinogenesis. We concluded iNOS and COX-2 inhibitor have good effects on gastric carcinogenesis.

Kinetic modeling and dynamic simulation for the catalytic wet air oxidation of aqueous ammonia to molecular nitrogen

Deuk Ki Lee,Jeong Shik Cho,Taejong Yu,Yong Su Lee,Jae Wan Choe,Sang Soo Lee 한국화학공학회 2016 Korean Journal of Chemical Engineering Vol.33 No.11

A kinetic model for the catalytic wet air oxidation of aqueous ammonia over Ru/TiO2 catalyst was developed considering the consecutive reaction steps as follows: (i) formation of active oxygen sites O* by the dissociative adsorption of aqueous O2 on the catalyst, (ii) oxidation of aqueous NH3 by the reaction with three O* sites to produce HNO2, (iii) aqueous phase dissociation of HNO2 into H+ and NO2 −, (iv) formation of NH4 + by the association of NH3 with the HNO2-dissociated H+, (v) formation of N2 by the aqueous phase reaction between NO2 − and NH4 +, (vi) formation of NO3 by the reaction of NO2 − with an O* site. For each reaction step, a rate equation was derived and its kinetic parameters were optimized by experimental data fitting. Activation energies for the reactions (ii), (v), and (vi) were 123.1, 76.7, and 54.5 kJ/mol, respectively, suggesting that the oxidation reaction of aqueous NH3 to HNO2 was a ratedetermining step. From the simulation using the kinetic parameters determined, the initial pH adjustment of the ammonia solution proved to be critical for determining the oxidation product selectivity between desirable N2 and undesirable NO3 − as well as the degree of oxidation conversion of ammonia.

Comparision of Percutaneous Absorption of Methyl Nicotinate with Laser Doppler Velocimeter after Sodium Lauryl Sulfate, Cellophane Tape-Stripping and Delipidization Induced Skin Irritations

Lee, Jeong Deuk,Lee, Sang Chin,Kim, Hyung Ok,Kim, Tae Yoon,Kim, Chung Won CATHOLIC MEDICAL CENTER 1992 Bulletin of the Clinical Research Institute Vol.20 No.1

This study was designed to characterize the difference of pharmacokinetics in percutaneous absorption of methyl nicotinate (MN) between normal and damaged skin. After topical application of 10 mM MN for 1.5 minutes on the irritated skin sites, we measured the cutaneous blood flow employing laser Doppler velocimeter (LDV) in the following time sequence; 0 (immediately after topical MN application), 1, 3, 5, 10, 15, 20, 30, 40, 50, and 60 minutes. We could find out the percutaneous absorption pattern of MN through this measurement of blood flow. The results were as follow: 1. The 5% sodium lauryl sulfate (SLS), cellophane tape-stripping and delipidization of stratum corneum have induced skin irritation and the cutaneous blood flow monitored with LDV has increased, comparing with normal control group (p<0.05). 2. In 5% SLS-irritated skin group, the percutaneous absorption of MN, as compared with normal control, was significantly increased until 5 minutes (p<0.05). But after 5 minutes, there was no statistical difference in percutaneous absorption between the SLS-irritated and the normal control group. 3. In cellophane tape-stripped skin group, the percutaneous absorption of MN was significantly increased up to 1 minute, and after 3 minutes, it decreased much more significantly than the normal control group (p< 0.05). 4. In delipidized skin group with acetone/ether, ns compared with normal control gorup, the percutaneous absorption of MN was significantly decreased between 5 minutes and 15 minutes (p<0.05), but there was no significant statistical difference in percutaneous absorption of MN at any other time. 5. Among cellophane tape-stripped skin group, delipidized skin group, and normal control group, the cellophane tape-stripped skin group got to the peak response time of blood flow earlier than other two groups, therefore MN was absorbed most rapidly in the cellophane tape-stripped skin group. These results suggested that, in percutaneous absorption, stratum corneum is the most important rate limiting step and removal or irritation of the stratum corneum caused the increased and rapid absorption of MN. It is known that stratum corneum is the main barrier to the percutaneous absorption of various chemicals (1). Many previous studies showed that percutaneous absorption increased after the deterioration of barrier function, due to irritations or diseases (2~4). But most studies of the percutaneous absorption have been performed on intact skin because of the difficulty of eonducting studies on diseased human skin and also the lack of comparable diseases in laboratory animals. In order to fgcilitate accurate determination of the potential for increased absorption through irritated skin and to investigate the role of stratum corneum and its lipid component in the percutaneous absorption, we experimentally induced irritation on the velar surface of the forearm of 10 healthy volunteers. For this purpose, we used three basic methods; patch-testing with 5% sodium lauryl sulfate (SLS) for 24 hours, stripping the skin with cellophane tape and delipidizing stratum corneum with acetone/ether mixture (1:1 in v/v). After above irritations, we sequently measured the percutaneous absorption of MN, which induced vasodilation of cutaneous vessel, with LDV.

Annular Atrophic Lichen Planus

Lee, Jeong Deuk,Lee, Chang Nam,Kim, Ji Eun,Cho, Sang Hyun 대한피부과학회 2003 Annals of Dermatology Vol.15 No.4

Annular atrophic lichen planus(AALP) is the most unusual variant of lichen planus that results from elastolytic activity of inflammatory cells. We report a case of AALP in 22-year-old man who had two annular pruritic plaques on the axilla. He had a two year history of skin lesions. Each lesion had elevated erythematous annular border with hyperpigmented atrophic macular center. Histopathologically, it showed typical features of lichen planus in the border of the lesion while a pattern of resolved lichen planus in its center. Elastic fibers was not found in the papillary dermis either at the border or at the center of the lesion.

Unusual presentations of papular umbilicated granuloma annulare(초)

( Jeong Deuk Lee ),( Min Ho Kim ),( Sang Hee Cha ),( Sang Hyun Cho ) 대한피부과학회 2010 초록집 Vol.48 No.10