사회적 음주자에서 날트렉손 투여가 급성 음주 반응에 미치는 영향

김종현,김성곤,신성현,박제민,김명정 大韓神經精神醫學會 2004 신경정신의학 Vol.43 No.6

Objectives : We investigated the effects of naltrexone on acute alcohol response, stimulant and sedative, in healthy social drinkers using two doses of alcohol intake. Methods : Twenty four healthy male medical students were voluntarily participated. The experimental method was Cross-over design, Subjects received 25 mg/day or 50 mg/day of naltrexone on the experimental days. Biphasic Alcohol Effects Scale (BAES), alcohol craving, and blood alcohol concentration (BAC) were measured before drinking and at 15, 30, 45, 60, 90,and 120 min after drinking. Results : 1) Group of 0.6 mg/kg of alcohol intake. When the scores of stimulative subscale of BAES were compared bet-ween the naltrexone and control group, the scores were significantly lower in the naltrexone group at 15 and 90 min after drinking. Alcohol induced sedative effect was significantly higher in the naltrexone group at 90 min after drinking. The alcohol induced alcohol craving at 45 and 60 min after drinking was significantly lower in the naltrexone group as compared to the control. 2) Group of 0.3 ing/kg of alcohol intake. The alcohol induced stimulative effect evident in the control group seen in the time span of 15 to 45 min after drinking was not seen in the naltrexone group. The increase of alcohol induced alcohol craving noticed at 30 min after drinking in the control group was not seen in the naltrexone group. BAC at 15 min after drinking was lower in the naltrexone group compared to the control. Conclusion : Naltrexone is suggested to attenuate stimulative effect, to intensify sedative effect, and to block alcohol induced alcohol craving. These triple actions might be utilized for treatment and prevention of relapse of alcohol dependence.

테니스 양손 백핸드 스트록의 3차원 운동학적 분석

신제민 한국스포츠리서치 2004 한국 스포츠 리서치 Vol.15 No.6

The purpose of this study was to analysis 3-d kinematic characteristics of two hand backhand stroke in tennis, 3 skilled subjects who were performed 3 successful trials were filmed with three dimensional cinematography. Kinematic variables computed were three dimensional displacements of racquet and angles of joints participated in the stroke. The 3-d angles were calculated by vector projected with the least squares solution in order to de fine complex joint movements, specially shoulder joints. The following conclusion were reached; 1. The direction of racquet at the impact was reverse to the aimed direction of a ball in the transverse plane. The role of trajectory of racquet after impact was also important to decide the ultimate direction of ball. 2. The angular motion in the horizontal adduction/abduction of the shoulder joints was affected to axial rotation of trunk the angle of internal/external rotation in the shoulder joints increased around the impact without increasing until the impact. 3. The angles of elbows joint were flexed below 150˚ at the impact, not fully extending. 4. The angle of internal/external rotation of trunk was external of trunk was rotated over 90˚ at the impact while insignificant angular oration in the other planes. 5. The pattern of ante of knee joints showed variability within subjects and trials without typical angular pattern during the total movement phase. But the magnitude and time of peak flexion were appeared before the impact at the left bide. 6. Both of foots were closed over 180˚ from the base line among local as at the impact. The right foot was much closed than left foot at impact as well as all of the phase, and almost keep fixed hat changing the angle until the impact.

태권도 옆차기와 돌려차기의 3차원 운동형태의 비교

신제민,진영완 한국운동역학회 2000 한국운동역학회지 Vol.9 No.2

The study compared to skills of Yeopchagi and Dolyeochagi in Taekwondo with the 3d kinematic analysis. To compare both of two skills, the study was analyzed as the relative time during the skills performed, the kinematic characteristics of individual segment, the relationship of inter-segment and the ranges of motion in 3d space. A performance time was mort shortened Dolyeochagi than Yeopchagi. A relative time that the time minimized angle of shank was later Dolyeochagi than Yeopchagi. The characteristic of kinematic of Individual segments showed clearly different in the flexion/extension plane. The angle of thigh increased continuously at an impact in Yeopchagi, but the angle stopped or decreased in Dolyeochagi at that time. The relationship of inter-segment showed difference largely between two skills in the flexion/extension plane, but the other plane showed no difference. These results were due to anatomical functional difference between two joints. The ranges of motion in 3d space were different at the abduction/adduction and the internal/external rotation.

남성화 암쥐의 모리스 수중 미로 수행력 : 성에 따른 공간기억력과 테스토르테론 효과의 차이

신성현,박제민,정우영,박문일,서유나,김성곤,강철중 大韓神經精神醫學會 2007 신경정신의학 Vol.46 No.5

Objectives : It is widely accepted that gonadal steroids are involved in organization and activation of sexual dimorphism in the brain areas related with spatial leaming. This study aimed to test the following hypothesis : 1) spatial memory is affected by sex, 2) this difference is related with brain differentiation by exposure to testosterone in the neonatal period, and/or 3) the sexual difference of spatial memory is the result of the interaction between organization of sexual dimorphism in the brain and the circulating testosterone after growing up. Methods : Testosterone enanthate 100 ㎍, ('androgenized') orvehicle ('control') was randomly administered to 28 female and 25 male rat pups within 24 hour of birth. The escape latencies (EL) in Morris water maze were measured in 15 and 16 week of age with vehicle ('baseline') and with 10 μmol/kg of testosterone ('with testosterone'), respectively. Each session Consis-ted of 10 trials, and means of the last 5 trials were analyzed. Results : The baseline EL of the males were significantly shorter than females regardless ofneonatal exposure to testosterone. With testosterone, EL of the androgenized females were significantly shorter than control females and comparable to those of males. Conclusion : Neonatal exposure to testosterone in female rats may develop a tesosterone-dependent male-like spatial leaming system.

기술수준에 따른 태권도 돌려차기 기술의 협응과 제어형태 비교

신제민,김로빈 한국운동역학회 1999 한국운동역학회지 Vol.9 No.1

The early studies of kicking skills in Taekwondo were simple two dimensional kinematic skills. But the later studies focused on three dimensional kinetic skills. These studies have acquired a lot of data concerned with kicking skills. These studies tended to investigate patterns of movement with comparison of between-skill or in-skill. The studies of movement patterns could systematically explain in motor controls which was focused on a mechanism of a skilled motor behavior. This study analyzed a pattern of Doelyochagi movement by 3D cinematograph and approached the movement as function of skill level in the view of motor control. The researcher selected six novices and six experts, and analyzed the change of coordination and control patterns with kinematic data. While the coordination variables were qualitatively analyzed by angle to angle graphs, control variables were qualitatively analyzed by angles. And the control variables were divided into the spatial and temporal factors which were consisted of the positive and negative maximal values, and timing the positive and negative maximal values. The researcher used t-test in order to find significant differences between two groups among the control variables. As the results of analyzing the coordination variables, the angle to angle graphs of expert group showed clearly to be froze between two joints in the flexion/extension and abduction/adduction, and showed to be released in the internal/external rotation during the third phase. Angles as control variables revealed significant differences in the flexion/extension while there no differences in the other two planes in the hip joint. But all of planes on the knee joint showed significant differences between two groups. As indicated by analyses of angles, the segments of experts tended to be frozen in the flexion/extension and abduction/adduction, and to be released in the internal/external rotation during the third phase.

랫드에서 신우황청심원의 급성 및 아급성독성시험

오승민,남혜윤,김준수,연제덕,신대희,이진영,박대규,조명행,정규혁 성균관대학교 약학연구소 1998 成均藥硏論文集 Vol.10 No.1

The acute and subacute toxicity of New Woohwangchungsimwon(NWCH) which was used l-muscone as substitutive material of musk were investigated in S.D. rats. In intraperitoneal acute toxicity test, rats(Sprague-Dawley, SPF) were injected intraperitoneally with dosages of 0, 540, 750, 1,070, 1,500 and 3,000 ㎎/㎏. Body weights were significantly decreased at 540 ㎎/㎏ dose group in both sexes and abnormal autopsy findings were founded in both sexes at all treated groups. Intraperitoneal LD_50 of NWCH was 812.3 ㎎/㎏ in male and 872.3 ㎎/㎏ in female rats. In the subacute toxicity study, NWCH was administrated orally to both sexes of rats for 4 weeks as several doses(0, 320, 800 and 2,000 ㎎/㎏). There were neither dead animals nor significant changes of body weights during the experimental period. In addition, no differences were found between control and treated groups in clinical signs, urinalysis, hematology, serum biochemical analysis, and other findings. Above data strongly suggest that no observed adverse effect level of NWCH might be over 2,000 ㎎/㎏/day in this study.

주산기 백서에서 혈중 에스트로겐 농도와 공간 기억력과의 상관관계

박민성,박제민,신성현,한귀원,김명정,김성곤 大韓神經精神醫學會 2006 신경정신의학 Vol.45 No.5

Objectives : This study was designed to find possible effect of pregnancy and parturition on spatial memory, especially in relation to levels of estrogen during the third trimester and postpartal period in rats. Methods : 25 female Sprague Dawley rats were divided into pregnant group (N= 14) and control group (N= 11). Changes in spatial memory during 6 weeks including third trimester and postpartal period were measured using Morris water maze. Time to reach the platform in the maze was indicator of spatial memory. Serum estrogen level was measured on 1 week before delivery, postpartal day 1, and day 14. Results : Both groups showed gradual improvement in performance by trial days and weeks, but no significant difference was found between the two groups. However in the third trimester, pregnant group showed a trend of less achievement on 3 days of learning than control group. Serum estrogen levels did not differ significantly between groups over the 6 weeks of period. However there was positive correlation between serum estrogen level on postpartum day 1 and time to reach platform on postpartum week 2, and negative correlation between estrogen level on postpartum day 14 and latency to the platform on postpartum week 5. Conclusion : These results imply that changes in the serum estrogen level may have dual effects on the spatial learning in peripartal period. It is suggested that decline in cognitive function might occur either by failure of rapid decrease of estrogen, immediately after parturition, or retarded restoration of estrogen in later postpartal period.

중증 재생불량성 빈혈 환자의 동종골수이식 후에 병발한 간정맥폐색질환(Hepatic Veno-Occlusive Disease)의 임상 경과 및 혈액응고 인자들의 변화

이제환,이규형,최성준,민영주,김신,이정신,김상희,박찬정,지현숙,김우건 대한조혈모세포이식학회 1999 대한조혈모세포이식학회지 Vol.4 No.2

연구배경: 재생불량성 빈혈 경우에는 조혈모세포 이식 후 VOD의 발생에 관한 보고가 거의 없는 실정이다. 저자 등은 cyclophosphamide와 antithymocyte globulin으로 전처치 후에 동종골수이식을 시행한 재생불량성 빈혈 환자들을 대상으로 하여 VOD의 발생과 임상 양상, 혈액응고 인자들의 변화에 관하여 분석하여 보았다. 방법: 재생불량성 빈혈로 동종골수이식을 시행 받은 13예 환자를 대상으로 하였다. 임상적 기준에 따라 VOD를 진단하였으며 임상 경과에 따라서 경중을 분류하였다. Protein C, protein S, antithrombin Ⅲ, tPA, PAI-1 등의 혈액응고 인자들을 전처치 투여전, 골수 주입당일, 이식 후 제7, 14, 21일에 측정하였다. 결과: 7예(53.8%)에서 VOD가 발생하였으며, 경증 5예, 중등도 2예였다. VOD군에서 혈소판치가 20.000/㎕ 이상을 유지하는 데까지 걸리는 시간이 더 길었다(p=0.026). 또한 VOD군에서 적혈구 및 혈소판의 수혈 요구량이 각각 유의하게 많았다(p=0.025, 0.015). 혈액응고 인자들의 변화를 보면, 제 0일에 VOD군에서 PAI-1의 혈중 농도가 유의하게 더 높았다.(p=0.034). 결론: 재생불량성 빈혈환자에서도 조혈모세포 이식 후에 VOD의 높은 발생률을 관찰하였으며, 대부분이 경증이었다. VOD 환자에서의 혈액응고 인자들의 변화는 PAL-1이 VOD의 감별 진단을 위하여 이용될 수 있음을 시사하고 있다. Background: There are few reports about the occurrence of hepatic veno-occlusive disease(VOD) after bone marrow transplantation (BMT) for severe aplastic anemia (SAA). We analyzed the incidence and clinical outcomes of VOD and changes of coagulation factors in patients with SAA who were conditioned with cyclophosphamide plus ATG and underwent allogeneic BMT. Methods: Thirteen patients with SAA who underwent allogeneic BMT were included. A diagnosis of VOD was made according to clinical criteria, and severity of VOD was classified according to clinical course. Plasma levels of protein C, protein S, antithrombin Ⅲ, t-PA, and PAI-1 were determined before preparative chemotherapy, in the day of marrow infusion, and days 7, 14, and 21. Results: VOD occurred in 7/13(53.8%). Five were mild VOD and two were moderate VOD. Time to platelet ≥20,000/L was slower (p=0.026) and red cell and platelet transfusion requirements were higher (p=0.025,0.015) in patients with VOD . The mean plasma level of PAI-1 at 0 were higher in patients with VOD (p=0.034). Conclusion: We observed the high incidence of VOD after allogeneic BMT in patients with SAA. A majority of cases of VOD were mild. The changes of PAI-1 after BMT may be useful marker of hepatic VOD

미세가공기술을 이용한 Interdigitated Array 전극의 제작 및 응용

이강신,김태한,김승렬,신민철,이원용,박제균 경북대학교 센서기술연구소 1998 센서技術學術大會論文集 Vol.9 No.1

Interdigitated array (IDA) electrodes were fabricated using silicon technology for highly sensitive biosensing. Thin-film platinum and gold IDA electrodes were fabricated with 10 μm band width and 7 μm gap between the bands using photolithographic techniques. The IDA electrode has 100 fingers consisting of 50 finger anodes and 50 finger cathodes. By applying both the oxidation and the reduction potentials of a reversible redox couple, ferrocyanide, to the pairs of the IDA electrode, an increased current generation can be observed. In our experiment, the collection efficiency of IDA electrode was 46% and amplification factor was 12.6. This preliminary experiment implies that the IDA electrode could be applied to biosensor fabrications as the sensitive electrochemical transducer when a lower detection limit is needed such as phenol sensor for drinking water analysis.

치아 우식증 수복용 복합레진의 개발과 치아 적합성에 관한 연구 제 2 편 : 치아와의 결합양상 및 적합성에 관한 연구

임호남,민병순,신제원 大韓齒科器材學會 1992 대한치과재료학회지 Vol.19 No.2

The purpose of this study was the evaluations of bonding behavior of experimental resins (KM-22) to acid etched enamel and surface treated dentin using shear bond strength and microleakage test. Enamel surfaces etched with various concentrated phosphoric acid and dentin surface treated with ferric compound were bonded with resin materials and used to measure the shear bond strength between them. To test the microleakage, tooth etched and filled with resins were cross cut and observed the degree of penetration of dye into interfaces. Following conclusions were drawn : 1. All of phosphoric diluted with various concentrations etching the enamel surfaces increased the shear bond strength of experimental resins to enamel, and acid concentrations over 30% did not make the significant higher bond strength of resins to etched enamel with it. 2. Some pretreatment of dentin smear layers increased the shear bond strength between dentin and resins. 3. Substantial leakage occurs around unetched composite restorations, and the filler added experimental resins showed the decresed penetration of the dye. 4. From dentin surfaces treated with EDTA, removal of smear layers and exposure of dentinal tubules were confirmed, but dentinal tubules of ferric oxalate treated dentin were occluded with some crystals regarded as precipitates.