류마티스 관절염환자의 혈청 Interleukin-10

유빈 ( Bin Yoo ),박재경 ( Jae Kyoung Park ),오원일 ( Won Il Oh ),오순환 ( Sun Whan Oh ),문희범 ( Hee Bom Moon ) 대한류마티스학회 1997 대한류마티스학회지 Vol.4 No.1

Objective: To investigate whether the serum levels of IL-10 in patients with rheumatoid arthritis are different from those of normal controls and SLE patients and to find out any correlation with disease activity parameters of rheumatoid arthritis. Methods: Sera from 20 healthy normal persons, 16 rheumatoid arthritis patients and 16 patients with systemic lupus erythematosus were collected and measured for IL-10 and IL-6. Various disease activity parameters were measured in RA patients. Results: The serum level of IL-10 in RA patients was significantly elevated compared to normal controls but lower than those of SLE patients. In RA patients there was no definite correlation between the disease activity parameters and serum IL-10 levels. Despite significant improvements in terms of various disease activity parameters, there was no significant change of serum IL-10 levels after treatment in RA patients. In seropositive RA patients, positive correlation was found between serun IL-10 and rheumatoid factor levels. Conclusion: Our findings show that the serum IL-10 levels in patietns with RA are elevated compared to normal controls but lower than those of SLE patients. There was no correlation between serum IL-l0 levels and disease acivity parameters of RA.

대용량 텍스트 데이터베이스를 위한 효율적인 2단계 합성 요약 화일 방법

유재수(Yoo Jae Soo),강형일(Kang Hyung Il) 한국정보처리학회 1997 정보처리학회논문지 Vol.4 No.4

In this paper, we propose a two-level hybrid signature file method(THM) to efficiently deal with large text database that use a term discrimination concept. In addition, we apply Yoo's clustering scheme to the two-level hybrid signature file method. The clustering scheme groups similar signatures together according to the similarity of the highly discriminatory terms so that we may achieve better performance on retrieval. The space-time analytical model of the proposed two-level hybrid method is provided. Based on the analytical model and experiments, we compare it with the existing methods, i.e., the bit-sliced method(BM), the two-level method(TM), and the hybrid method(HM). As a result, we show that THM achieves the best retrieval performance in a large database with 100,000 records when the number of matching records is less than 160.

반복적 관절내 출혈이 관절 활막과 연골 세포의 변화에 미치는 영향

유명철(Myung Chul Yoo),조윤제(Yoon Jae Cho),김강일(Kang Il Kim),전성욱(Sung Wook Chun),소동혁(Dong Hyuk So),조형준(Hyung Jun Cho),양형인(Hyung-In Yang),이상훈(Sang-Hoon Lee),이연아(Yeon-Ahn Lee) 대한정형외과학회 2008 대한정형외과학회지 Vol.43 No.3

목적: 혈액의 관절강내 반복주사 동물 모델을 이용하여, 혈우병성 관절염에서 활막의 변화와 연골 파괴에 대한 병리 기전과 경과 과정을 연구하였다. 대상 및 방법: 뉴질랜드 백색 토끼 수컷 20마리에 18주간 1주 3회씩 귀에서 자가 혈액을 채취하여 우측 슬관절에 1 ㎖를 주사하고, 좌측 슬관절에 생리식염수 1㎖를(대조군) 주사하였다. 11주 후와 18주 후에 양측 슬관절의 X선 촬영을 시행하였고, 활액막과 연골을 채취하여 조직검사를 시행하였다. 활막 세포 배양에서 RT PCR를 이용한 cytokine의 변화를 관찰 하였고, 연골 세포를 추출 배양하여 GAG 및 PGE₂, MMP-1,3 생성을 측정하였다. 결과: 11주째 육안적 변화는 없었으나, 우측 슬관절의 활액막은 조직학적으로 경한 증식반응과 형질세포 및 단핵세포의 활액막내 침착을 보이며 약간의 염증 소견을 나타냈다. 단순 방사선 소견상 특이 소견은 발견되지 않았다. 18주 후에는 육안적으로 우측 슬관절이 좌측에 비하여 부어 있었고, 단순 방사선학적 소견에서는 관절의 퇴행성 변화가 관찰 되었다. 병리학적 소견은 활막의 심한 증식과 염증세포의 침윤이 관찰되었다. 활막의 real-time RT PCR 시행결과 TNF-alpha, IL-1, MMP-1 mRNA 발현이 증가되었다. 연골세포 배양에서 대조군과 비교시 GAG 생성은 감소하고 PGE₂는 증가하였으며 MMP-1과 MMP-3는 변화가 없었다. 결론: 반복적인 관절 내 출혈은 활막세포를 자극하여 활막의 심한 증식과, proinflammatory cytokines의 생성을 증가시키며, 이는 연골 세포의 재생을 억제하고 연골세포의 염증을 증가시켜 연골의 대사기능이 억제되면서 퇴행성 변화를 촉진하여 점차적으로 관절염으로 이행되는 것으로 사료된다. Purpose: We designed this study to demonstrate the pathophysiology of hemophilic arthropathy (HA) by creating an animal model for determining the effect of repeated intraarticular bleeding in the synovium and articular cartilage. Materials and Methods: 20 normal male New Zealand white rabbits were used for this study. We injected 1 ㎖ of autologous blood from the ear vein of the rabbits into the right knee joint three timeds a week for 18 weeks, and we injected 1 ㎖ of normal saline into the left knee joint three times a week for 18 weeks as a control group. We examined the pathologic changes by microscopy and plain X-ray, and we determined the mRNA expression of proinflammatory cytokines in the synovium of the HA by performing real time RT-PCR at the 11<SUP>th</SUP> week and 18<SUP>th</SUP> week after starting blood-injection. We also examined the GAG and the PGE2 production in cultured chondrocytes that were extracted from the HA knees. Results: At the 11<SUP>th</SUP> week, after blood injection there were no remarkable gross changes in the HA knees and the control knee joints. At the 18<SUP>th</SUP> weeks, the experimental knee joints (HA knees) showed grossly swelling and degenerative changes by X-ray. The infiltration of inflammatory cells and the synovial proliferation in the HA knee joints were compared with that in the control knee joints by microscopic examination. The expressions of the mRNA of TNF-alpha, IL-1, MMP-1 and MMP-3 in the HA synovium were increased, as determined by real time RT-PCR, as compared with that in the control knee. In the cultured chondrocytes, the GAG production was decreased and the PGE2 was increased, but the MMP-1 and MMP-3 were not changed, as determined by ELISA. Conclusion: Our results showed that the GAG production of chondrocytes of the HA knees was decreased and there was increased PGE2, so that the cartilage degeneration by intra-articular bleeding was caused by the decreased metabolism of chondrocytes rather than by increased catabolism of the chondrocytes. We suggest that HA was associated with synovitis and cartilage degeneration, but decreased cartilage metabolism was the major mechanism of HA.

Characterization of effector memory CD8+ T cells in the synovial fluid of rheumatoid arthritis.

Cho, Bon-A,Sim, Ji Hyun,Park, Ji Ah,Kim, Hye Won,Yoo, Wan-Hee,Lee, Seung-Hyun,Lee, Dong-Sup,Kang, Jae Seung,Hwang, Young-Il,Lee, Wang Jae,Kang, Insoo,Lee, Eun Bong,Kim, Hang-Rae Springer 2012 Journal of clinical immunology Vol.32 No.4

<P>Little is known about the cellular characteristics of CD8(+) T cells in rheumatoid arthritis (RA). We addressed this by investigating whether the frequency of the CD8(+) T cell subsets and their phenotypic characteristics are altered in the peripheral blood and synovial fluid (SF) from patients with RA. In this study, CD8(+) T cells, mainly CD45RA(-) effector memory (EM) CD8(+) T cells, were increased significantly in the SF, but not in the peripheral blood from RA patients, compared with healthy controls. The synovial EM CD8(+) T cells were activated phenotypes with high levels of CD80, CD86, and PD-1, and had a proliferating signature in vivo upon Ki-67 staining, whereas the Fas-positive cells were prone to apoptosis. In addition, EM CD8(+) T cells in the SF were less cytotoxic, as they expressed less perforin and granzyme B. In particular, the proportions of synovial fluid mononuclear cells that were CCR4(+)CD8(+) T cells and IL-4-producing CD8(+) T cells (i.e., Tc2 cells) were significantly higher than those in peripheral blood mononuclear cells of patients with RA and healthy controls. In addition, the number of IL-10-producing CD8(+) suppressor T (Ts) cells increased significantly in the SF of RA patients. Especially, CD8(+) T cells were inversely correlated with disease activity. These findings strongly suggest that EM CD8(+) T cells in the SF are increased, likely because of inflammation, and they may be involved in modulating inflammation, thereby affecting the development and progression of RA.</P>

기술가치 평가모델의 비교 및 분석

兪日根,李在勳 弘益大學校 科學技術硏究所 2001 科學技術硏究論文集 Vol.12 No.-

Since the technology is one of the important factor for the development of countries or companies, evaluation of technology became essential for investment on venture company, technology trading and M&A. The appropriate evaluation of technology is so important yardstick of competence of business that, in this thesis, the theories and the present conditions of technology evaluation model in several institutes are compared and analysed and also the desirable direction for the future right model are suggested. Technology evaluation model is basically composed of three parts: cost-market-income approach method, technology factor method, real option method. We have to choose proper model among several methods because there are various technology evaluation methods and they have different merits and demerits. So that, due to some limits for each model, there is no complete one which can drive the right valuation by itself at one time. Thus, right valuation seems to be required that several different methods of each model should be applied and analysed at the same time and then decide the right answer that satisfies the suggested conditions.

국문학의 열린 길 찾기 : 연구와 교육의 몇 가지 문제를 중심으로

유재일 청주대학교 인문과학연구소 2000 人文科學論集 Vol.22 No.-

이 글은'인문학의 위기에 관한 한 단방 처방이 없다는 것'을 전제로 삼았다. 왜냐하면 학내외의 여러 곳에서 인문학 여러 분야의 전공자들이 그 위기를 극복할 수 있는 각자의 의견을 제시해 해결책을 찾아보고,그 모여진 바람직한 해결책들이 제도로 가시화될 때,인문학은 더욱 가치 있는 학문으로 발저하리라고 생각되기 때문이다.

Syntactic-Semantics 방법에 신경망을 결합한 온라인 한글 필기체 인식

박두일,조범석,유재휘 명지대학교 공학기술연구소 1993 공학기술연구소 논문집 Vol.8 No.-

This paper presents a cursive on-line handwritten Hangul recognition model which permits cursive writing between graphemes. Add semantics to the basic stroke types possessed by graphemes and expand stroke types. Also, by the design of attribute grammer more accurate feature values will be elicited. The combination of the feature values with genetic algorithm and BP will improve the neural networks model, and this type of learning will increase the recognition rate and reduce the learning time. In this paper, this new on-line handwritten Hangul recognition model is proposed.

이규보의 영물시에 나타난 즉물적 개방성에 대하여

유재일 연세대학교 국어국문학과 1980 연세어문학 Vol.13 No.-

상재하중 하의 Soil Nailing System의 모형실험

유남재,유건선,김재일 江原大學校 産業技術硏究所 1994 産業技術硏究 Vol.14 No.-

This research is an experimental work of investigating the behavior of soil nailing system under surcharges whereas most of them were concentrated on evaluating its capacity under selfweight of excavated ground. Model tests in laboratory were performed to investigate the ultimate bearing capacity of soil nailing system under surcharges in forms of strip loading. Tests were carried out to find parameters controlling its capacity such as length of nail, vertical and horizontal spacings between nails, inclination of nail installation and loading position of surcharges. Failure mechanism of forming failure line due to surcharge to soil nailing system was also observed by using dyed sand and monitoring its behavior. From results of these test, effects of parameters was analyzed qualitatively. Thus, this experimental results could provide meaningful data to analyze and design this system later.

Low doses of ionizing radiation suppress doxorubicin-induced senescence-like phenotypes by activation of ERK1/2 and suppression of p38 kinase in MCF7 human breast cancer cells

신재식,우상혁,이형찬,홍승우,유두현,홍석일,이왕재,이명석,진영우,안성관,진동훈,박인철 Sookmyung Women's University Research Institute of 2010 여성과 건강 Vol.6 No.1