Regulation of short-distance transport of RNA and protein

Kim, Jae-Yean,Rim, Yeonggil,Wang, Jing,Jackson, David Plant molecular biology and biotechnology research 2005 Plant molecular biology and biotechnology research Vol.2005 No.

The intercellular trafficking of proteins and RNAs has emerged as a novel mechanism of cell-cell communication in plant development. Plasmodesmata (PD), intercellular cytoplasmic channels, have a central role in cell-cell trafficking of regulatory proteins and RNAs. Recent studies have demonstrated that plants use either a selective or a non-selective PD trafficking pathway for regulatory proteins. Moreover, plants have developed strategies to regulate both selective and non-selective movement. Recent work has focused especially on integrating the recent understanding of the function and mechanisms of intercellular macromolecule movement through PD.

A novel cell-to-cell trafficking assay indicates that the KNOX homeodomain is necessary and sufficient for intercellular protein and mRNA trafficking.

Kim, Jae-Yean,Rim, Yeonggil,Wang, Jing,Jackson, David Cold Spring Harbor Laboratory in association with 2005 Genes & development Vol.19 No.7

<P>Cell-to-cell trafficking of regulatory proteins is a novel mechanism for communication during cell fate specification in plants. Although several developmental proteins traffic cell-to-cell, no signals that are both necessary and sufficient for this function in developmental proteins have been described. We developed a novel trafficking assay using trichome rescue in Arabidopsis. Fusion to KNOTTED1 (KN1) conferred gain-of-trafficking function to the cell-autonomous GLABROUS1 (GL1) protein. We show that the KNOX homeodomain (HD) is necessary and sufficient for intercellular trafficking, identifying a novel function for the HD as the minimal sequence required for trafficking of KN1 and its associated mRNA.</P>

Validation of Usefulness for Korean Paper and Pencil Test and Computer Based Stroop Test to Detect Minimal Hepatic Encephalopathy in Korea

( Jae Yoon Jeong ),( Dae Won Jun ),( Daiseg Bai ),( Ji Yean Kim ),( Sang Bong Ahn ),( Sang Gyune Kim ),( Hyoung Su Kim ),( Soung Won Jeong ),( Yong Kyun Cho ),( Do Seon Song ),( Hee Yeon Kim ),( Young 대한간학회 2017 춘·추계 학술대회 (KASL) Vol.2017 No.1

Aims: For multicenter studies, the diagnosis of minimal hepatic encephalopathy (MHE) by consensus should utilize at least two of the current validated testing strategies: paper-pencil test and one computerized test. Recently we developed a ‘Korean paper and pencil test’ (K-PPT) and a mobile base ‘Korean Stroop test’ (K-stroop test) as a ‘copyleft’. The aim of this study is to validate the usefulness for K-PPT and K-stroop test to detect MHE in Korea. Methods: One hundred thirty four patients with hepatitis B virus associated cirrhosis without overt HE underwent K-PPT (number connection test-A, number connection test-B, digit span test-forward, digit span test-backward and symbol digit modality test), psychometric hepatic encephalopathy score (PHES) and mobile based K-Stroop test. MHE was defined as an impaired performance under - 1.5 standard deviations from the mean on more than 2 tests in K-PPT. And we defined MHE as ≤ -5 points in PHES. Results: Prevalence of MHE in enrolled patients was 22.4% using K-PPT and 20.9% using PHES. The agreement of MHE diagnosis between K-PPT and PHES was 86.6% (sensitivity 71.4%, specificity 90.6%, k coefficients=0.605, P<0.0001). ‘Off time’ (P=0.001), ‘On time + Off time’ (P=0.007) and ‘On time - Off time’ (P=0.041) of K-stroop test were significantly different between patients with MHE and those without MHE diagnosed using PHES. The number of impaired performance of variables in K-PPT (r=-0.697, P<0.0001) and off-time of K-stroop test (r=-0.405, P<0.0001) showed linear correlation with conventional PHES test. Conclusions: K-PPT and mobile based K-stroop test have good correlation with conventional PHES to detect MHE in patients with cirrhosis.

A novel cell-to-cell trafficking assay indicates that the KNOX homeodomain is necessary and sufficient for intercellular protein and mRNA trafficking

Kim, Jae-Yean,Rim, Yeonggil,Wang, Jing,Jackson, David Plant molecular biology and biotechnology research 2005 Plant molecular biology and biotechnology research Vol.2005 No.

Cell-to-cell trafficking of regulatory proteins is a novel mechanism for communication during cell fate specification in plants. Although several developmental proteins traffic cell-to-cell, no signals that are both necessary and sufficient for this function in developmental proteins have been described. We developed a novel trafficking assay using trichome rescue in Arabidopsis. Fusion to KNOTTED1 (KN1) conferred gain-of-trafficking function to the cell-autonomous GLABROUS1 (GL1) protein. We show that the KNOX homeodomain (HD) is necessary and sufficient for intercellular trafficking, identifying a novel function for the HD as the minimal sequence required for trafficking of KN1 and its associated mRNA.

Plant volatiles as method of communication

Jae-Yean Kim,이숙희,현태경,김선원,김재연 한국식물생명공학회 2013 Plant biotechnology reports Vol.7 No.1

Plants emit volatile compounds that can act as a communication method to insects, neighboring plants and pathogens. Plants respond to leaf and root damage by herbivores and pathogens by emitting these compounds. The volatile compounds can deter the herbivores or pathogens directly or indirectly by attracting their natural enemies to kill them. The simultaneous damage of plants by herbivores and pathogens can influence plant defense. The induced plant volatiles can also make neighboring plants ready for defense or induce defense in parts distant from the damaged area of the same plant. Belowground root herbivory can alter the defense response to aboveground leaf herbivory. In addition, most plants normally emit volatile compounds from their flowers that directly attract foraging mutualistic insects for nectar, which in turn perform the very important function of pollination for subsequent reproduction. The volatile compounds emitted from the floral and vegetative parts of plants belong to three main classes of compounds: terpenoids, phenylpropanoids/ benzenoids, and C6-aldehydes (green-leaf volatiles). The volatile phytohormones methyl salicylate and methyl jasmonate serve as important signaling molecules for communication purposes, and interact with each other to optimize the plant defense response. Here we discuss and integrate the current knowledge on all types of communication between plants and insects, neighboring plants and pathogens that are mediated through plant volatiles.

Phosphorylation of the transcriptional repressor MYB15 by mitogen-activated protein kinase 6 is required for freezing tolerance in <i>Arabidopsis</i>

Kim, Sun Ho,Kim, Ho Soo,Bahk, Sunghwa,An, Jonguk,Yoo, Yeji,Kim, Jae-Yean,Chung, Woo Sik Oxford University Press 2017 Nucleic acids research Vol.45 No.11

<P><B>Abstract</B></P><P>The expression of <I>CBF</I> (C-repeat-binding factor) genes is required for freezing tolerance in <I>Arabidopsis thaliana. CBFs</I> are positively regulated by INDUCER OF CBF EXPRESSION1 (ICE1) and negatively regulated by MYB15. These transcription factors directly interact with specific elements in the <I>CBF</I> promoters. Mitogen-activated protein kinase (MAPK/MPK) cascades function upstream to regulate <I>CBFs</I>. However, the mechanism by which MPKs control <I>CBF</I> expression during cold stress signaling remains unknown. This study showed that the activity of MYB15, a transcriptional repressor of cold signaling, is regulated by MPK6-mediated phosphorylation. MYB15 specifically interacts with MPK6, and MPK6 phosphorylates MYB15 on Ser168. MPK6-induced phosphorylation reduced the affinity of MYB15 binding to the <I>CBF3</I> promoter and mutation of its phosphorylation site (MYB15<SUP>S168A</SUP>) enhanced the transcriptional repression of <I>CBF3</I> by MYB15. Furthermore, transgenic plants overexpressing MYB15<SUP>S168A</SUP> showed significantly reduced <I>CBF</I> transcript levels in response to cold stress, compared with plants overexpressing MYB15. The <I>MYB15<SUP>S168A</SUP></I>-overexpressing plants were also more sensitive to freezing than <I>MYB15</I>-overexpressing plants. These results suggest that MPK6-mediated regulation of MYB15 plays an important role in cold stress signaling in <I>Arabidopsis</I>.</P>

Comparison of total energy intakes estimated by 24-hour diet recall with total energy expenditure measured by the doubly labeled water method in adults

Kim Eun-Kyung,Fenyi Justice Otoo,Kim Jae-Hee,Kim Myung-Hee,Yean Seo-Eun,Park Kye-Wol,Oh Kyungwon,윤성하,Ishikawa-Takata Kazuko,Park Jonghoon,Kim Jung Hyun,Yoon Jin-Sook 한국영양학회 2022 Nutrition Research and Practice Vol.16 No.5

BACKGROUND/OBJECTIVES The doubly labeled water (DLW) method is the gold standard for estimating total energy expenditure (TEE) and is also useful for verifying the validities of dietary evaluation tools. In this study, we compared the accuracy of total energy intakes (TEI) estimated by the 24-h diet recall method with TEE obtained using the doubly labeled water method. SUBJECTS/METHODS This study involved 71 subjects aged 20–49 yrs. Over a 14-day period, three 24-h diet recalls per subject (2 weekdays and 1 weekend day) were used to estimate energy intakes, while TEE was measured using the DLW method. The paired t-test was used to determine the significance of differences between TEI and TEE results, and the accuracy of the 24-h recall method was determined by accuracy predictions percentage, root mean square error, and bias. RESULTS Average study subject age was 33.4 ± 8.6 yrs. The association between TEI and TEE was positive and significant (r = 0.463, P < 0.001), and the difference between TEI (2,084.3 ± 684.2 kcal/day) and TEE (2,401.7 ± 480.3 kcal/day) was also significant (P < 0.001). In all study subjects, mean TEI was 12.0% (307.5 ± 629.3 kcal/day) less than mean TEE, and 12.2% (349.4 ± 632.5 kcal/day) less in men and 11.8% (266.7 ± 632.5 kcal/day) less in women. Rates of TEI underprediction for all study subjects, men, and women, were 60.5%, 51.4%, and 66.7%, respectively. CONCLUSIONS This study shows that 24-h diet recall underreports energy intakes. More research is needed to corroborate our findings and evaluate the accuracy of 24-h recall with respect to additional demographics.

Treatment of Hepatocellular Carcinoma with Portal Vein Thrombosis by Sorafenib Combined with Hepatic Arterial Infusion Chemotherapy

Mi Yean Yang,정승원,Dong Kyun Kim,김상균,장재영,Young Seok Kim,이준성,Boo Sung Kim,Jung Hoon Kim,Yong Jae Kim 거트앤리버 소화기연관학회협의회 2010 Gut and Liver Vol.4 No.3

Treatment with sorafenib prolongs both the median survival and time to progression by nearly 3 months in patients with advanced hepatocellular carcinoma. Although the effects of combining sorafenib therapy with other anticancer treatment modalities have not been clarified, combination treatment is strongly expected to be beneficial. We report the case of a 50-year-old man who exhibited a partial response and portal vein thrombosis (PVT) revascularization after sorafenib combined with hepatic arterial infusion chemotherapy (HAIC). He exhibited a decrease in tumor size and PVT after 2 months of sorafenib monotherapy. However, no additional response was seen during the subsequent 2 months. To achieve a better tumor response, we combined HAIC with sorafenib. Daily cisplatin (7 mg/m2 on days 1-5) and 5-fluorouracil (170 mg/m2 on days 1-5) were infused repeatedly every 4 weeks, and the sorafenib prescription was modified. After four cycles of combined therapy, both the tumor size and PVT were much improved and exhibited partial response.

스마트 농업의 현황과 발전 방향

김연중(Yean-Jung Kim),국승용(Seung-Young Gouk),김용렬(Young-Ryul Kim),이명기(Myung-Gi Lee),김종선(Jong-Sun Kim),김윤형(Yoon-Hyung Kim),민경택(Kyung-Taek Min),지인배(In-Bae Ji),심재헌(Jae-Hun Sim) 한국농촌경제연구원 2013 한국농촌경제연구원 정책연구보고서 Vol.- No.-

현대 농업은 전통적인 생산방식에서 벗어나 6차산업으로 범위를 확대하고 있으며, IT(정보통신)·BT(바이오)·ET(환경)·NT(나노) 등 융합기술과의 접목을 통해 고부가가치 융합산업으로 발전하고 있다. 특히 농업과 ICT의 융합은 농림업 생산·유통·소비의 전 과정에 ICT를 접목하여 신성장동력을 확충하고 새로운 가치를 창출하는 과정이며 고령화에 따른 노동력 부족, 기상이변, 복잡한 유통구조에 따른 고비용 구조, 급변하는 소비자 기호 변화 등 농업이 당면한 문제를 해결하는 방안으로 그 중요성이 커지고 있다. 본 연구는 스마트 농업의 현황, 개념 및 필요성을 정리하고 각 분야별 도입가능 기반 기술 및 국내외 사례 검토를 통해 스마트 농업 도입을 위한 정책 방향을 제시하는 데 그 목적이 있으며 주요 시사점 및 결론은 다음과 같다. 첫째, 원예 부문의 경우 스마트 기술은 현재 시설원예나 과수 부문에서 주로 생산비 절감, 노동 부담 절감, 고품질 생산과 품질관리 등에 초점을 맞추어 도입되고 있다. 향후 노지 채소 부문의 스마트 기술 개발 및 보급 정책이 필요하다. 둘째, 축산 부문 스마트 농업 확대를 위해서는 식품안전에 대한 정보의 구축과 전달이 잘 이루어질 수 있는 체계를 구축함으로써 생산과 유통단계에서 소비자들의 요구에 부응할 수 있어야 한다. 이를 위해 생산 부문의 IT를 이용한 가축 환경 모니터링·제어체계 및 성장환경 통합관제시스템을 통한 농장 자동화 시스템을 확대·구축할 필요가 있으며, 유통·가공에서의 IT를 이용한 축산물 물류정보 융합기반 유통 선진화 기술 도입이 필요하다. 셋째, 임업의 경우 스마트 기술은 임업 생산성을 높일 수 있는 계기가 될 수 있지만 이를 위해서는 산림경영에 필요한 정보 콘텐츠 구축, 정보통신 인프라구축, 앱 개발, 사용자 능력 배양 등이 시급한 선결 과제이다. 넷째, 농산물 유통과 물류 분야의 스마트 기술 도입을 위해서는 농산물의 표준·규격화가 확대되어야 하며, 품목별 표준은 농산물의 유통 특성을 충분히 반영해야 하므로 공동선별을 통해 농산물을 유통하는 조직의 합의를 통해 표준을 제정하는 것이 바람직하다. 다섯째, 농촌 활력을 위해 IT를 활용하여 농촌관광, 귀농·귀촌 지원, 농촌생활지원, 농촌교육, 농촌복지, 농촌자원의 효율적 활용과 수요자와 원활한 네트워크와 소통을 위해 첨단화된 농촌으로 탈바꿈하기 위한 정책적 배려가 필요하며, 이를 위해서는 농촌주민에 대한 IT 활용에 관한 교육과 지속적인 투자가 필요하다. 여섯째, 지리정보는 단일 산업보다는 다른 산업과 융복합하여 새로운 부가가치를 창출하는 파급효과가 크기 때문에 향후 스마트 농업 분야에서 융복합을 강화할 필요가 있다. 스마트 워터그리드의 경우는 인력 및 장비의 효과적인 운용과 물절약을 통한 농업용수의 효율적인 이용에 기여할 것으로 예상된다. 일곱째, 스마트 농업 확대 및 보급을 위해서 기존 농식품 분야 IT 융합 관련사업의 운영 및 활용 실태를 점검할 필요가 있으며, 농식품 IT 융합 분야의 정부 R&D 투자 확대 및 실용화 제고를 위해서는 R&D 투자의 기획단계에서부터 실용화를 목표로 기술개발이 이루어지는 R&BD(Research and Business Development) 방식의 추진체계가 적극적으로 이루어져야 한다.

상어 콜라겐의 항산화능, 항균성, Elastase 및 Tyrosinase 저행활성

김재원 ( Jae Won Kim ),김도균 ( Do Kyun Kim ),박진수 ( Jin Soo Park ),이예경 ( Ye Kyung Lee ),백경연 ( Kyung Yean Beik ),김순동 ( Soon Dong Kim ) 한국식품저장유통학회 ( 구 한국농산물저장유통학회 ) 2009 한국식품저장유통학회지 Vol.16 No.3