오렌지 껍질로부터 분리된 프라보노이드의 화학요법 감작효과

최철익,변익건,차윤정,김선필,민영돈,유진철,송재경 조선대학교 2001 The Medical Journal of Chosun University Vol.26 No.2

Background and Objectives : The overexpression of P-glycoprotein (Pgp) or multidrug resistanceassociated protein (MRP) confers multidrug resistance (MDR) to cancer cells. MDR cells could be sensitized to anticancer drugs when treated concomitantly with a chemosensitizer. In this study, orange peel have been screened for the development of chemosensitizers reversing MDR. Materials and methods : Expression of Pgp in AML-2/WT and AML-2/D100 were determined using western blot analysis and RT-PCR. Candidates for chemosensitizers were isolated, purified from orange peel using sephadex LH-20 column chromatography and silica-gel column chromatography, whose structure was determined using ^1H-NMR, ^13C-NMR, ^1H-^1H-COSY, 13C-NMR, EI-Mass, IR. Chemosensitizing effect of NP-28 on AML-2/DX100 over expressing Pgp and MRP was determined by the MTT assay. Membrane toxicity was evaluated by the RBC hemolysis test. Antioxidant effects were examined by using a fluorescence probe (2’-7’-dichlorofluorescein diacetate). Results : A potent candidate, NP-28, has a flavonoid nucleus with some methoxy moiety. NP-28 is believed to have high therapeutic index, being non-transportable inhibitor and possibly, of inhibiting other efflux pumps such as MRP and antioxidant effects. NP-28 is also unlikely to be a substrate of Pgp. NP-28 did not increase Pgp levels. Conclusion : NP-28 has a Chemosensitizing effect to reverse Pgp-mediated MDR by increasing the intracellular accumulation of drugs. Furthermore, they do not increase expression of Pgp, which can allow a long term use without the concern about Pgp activation. NP-28 is anticipated as an ideal second-generation flavonoid chemosensitizer and/or possibly other therapeutic agents by further investigation.

확률과정에 기초한 ERAM 이론의 재해석 및 검증

최재필,조형규,최현철,조영진 대한건축학회 2004 대한건축학회논문집 Vol.20 No.11

ERAM theory is an analytical tool for spatial configuration of architectural and urban spaces such as Space Syntax model. In this study, we tried (1) to reinterpret ERAM theory in terms of Markov process which is a kind of stochastic process and (2) to compare ERAM theory with Space Syntax model using pedestrian movement data. Besides, a new quantitative index ERAM and ERAM(r) for the spatial attribute is suggested. The results of the study include the followings. From the reinterpretation of ERAM theory, it can be said that ERAM theory is well compatible with stochastic process. From the comparison of integration and ERAM, it can be said that ERAM reflects the spatial configuration better than integration. Therefore, ERAM analysis can be used as a powerful tool for predicting accessibility of architectural and urban spaces.

數 種 韓藥의 아세틸콜린에스터레이즈 酵素活性에 대한 효과

최재환,김미영,김현미,최훈,전훈,김대근,임종필,임강현 대한본초학회 2002 大韓本草學會誌 Vol.17 No.1

아세틸콜린에스터레이즈(AChE)는 신경조직에 분포하는 효소로 신경전달물질 중 아세틸콜린(ACh)을 분해하는 효소로 시냅스 신호전달체계에 있어 중요한 역할을 하는 효소이다. 현재 알츠하이머병과 치매치료에 사용하는 서양약물은 AChE의 활성을 저해하는 작용기전을 가진다. 목적 : 전통적으로 기억력 감퇴, 치매 등 치료에 사용되어 온 한약의 AChE에 대한 작용을 밝히기 위해 본 실험이 구상되었다. 방법 : 생쥐 대뇌신경세포의 균질 혼합액으로부터 효소의 활성을 Ellman 방법을 개선한 엘리자(ELISA)법을 이용하여 측정하였다. 결과 : 실험결과 天花? 의 6종이 2.5㎎/㎖의 농도에서 우수한 효소활성저해력을 보였고, 50% 효소활성 저해농도(ID_50)를 구한 결과, 天花?의 ID_50은 1.5㎎/㎖이었고, ??? 1.2 ㎎/㎖, ?? 2.0 ㎎/㎖, ??草 1.2 ㎎/㎖, ??? 1.0 ㎎/㎖, ??生 1.7㎎/㎖, 및 大?皮 2.0 ㎎/㎖를 나타내어 忍冬?의 저해력이 가장 우수하였다. 결론 : 치매 및 기억력감퇴 증상을 치료하는 한약의 작용기전 중 AChE 효소활성억제효과는 忍冬? 의 6종에서 나타났으며, 이는 전통적인 한방치료에 과학적인 기초자료를 제공할 것으로 생각한다.

裁植距離가 輸出用 가지(Solanum melongena. L)의 生育 및 收量에 미치는 影響

최성관,이재필,진동호,오주성,박흥식,정순재 동아대학교 농업생명과학연구소 2001 農業生命資援硏究 Vol.10 No.1

This study was carried out to investigate effect of the planting distance of Eggplant(Solanum melongena. L). These grafting seeding "Torobambica", rootstock "Jang Ja" and "Chuk Yang" were tested to investigate the differences between the growth of eggplants and the numbers of eggplants which can be influenced by the planting distance such as 100×50cm, 100×60cm and 100×70cm. The results are summarized as follows: 1.The plant height, leaf length, leaf, width, leaf area and the number were good for the growth change of each cultivar from planting distance such as 100×70cm. 2.The grafting seedlings "Chuk YanglTorobambica" grew and developed better than "Jang Ja" and "Jang Ja/Torobambica" 3.Effects of scion on the rootstock for planting distance "Chuk Yang" grew and developed better than "Jang Ja"

국내 아파트 단위주호 평면의 공간 분석 : 1966년∼2002년의 서울지역 아파트를 대상으로

최재필,조형규,박인수,박영섭 대한건축학회 2004 대한건축학회논문집 Vol.20 No.6

This study intends to examine changes found in the unit floor plans of the apartments built in Seoul Metropolitan area from 1966 to 2002. The changes found in the unit floor plans are analyzed in the context of the Space Syntax Model and compared to the lifestyle and societal changes in Korea. The results of the study indicate the followings ; the unit floor plans have changed a lot from 3rd period to 4th period ; there is a tendency that design element in large unit such as numbers of bathrooms and bedrooms, etc. is imitated by small unit ; the unit floor plans of 3LDK apartments have been changing into the kitchen-dining-centered unit plans, but the unit floor plans of 4LDK apartments have hold down the living-centered unit plans ; there is a tendency that RRAs of master bedroom of 3LDK and 4LDK apartments have been decreasing.

해외 건축학교육 인증제도의 비교 분석

최재필 대한건축학회 2004 대한건축학회논문집 Vol.20 No.4

Because of WTO agreement with the consequent opening of architectural design market in Korea and because of UIA recommendation, the introduction of a new accrediting system for architectural education in Korea is unavoidable. In order to take advantage of the precedents, four overseas accrediting organizations were compared and analyzed, and as the results a few recommendations were made on how to establish the Korean architectural accrediting body. The conclusions include that the NAAB conditions and procedures are the best references to start with and that some of the NBAA guidelines may be adapted with regard to the relationship between accreditation system and the governmental administration.

인접행렬의 고유벡터 성분비를 이용한 공간 분석 : Spatial Configuration Analysis Using the Eigenvector Ratio of Adjacency Matrix

최재필,조형규,최현철,황용하 대한건축학회 2003 대한건축학회논문집 Vol.19 No.11

The purpose of the ERAM model proposed in this paper is to analyze the spatial importance immanent in the intrinsic structure of spaces. Use of the adjacency matrix representing the connection of spaces makes it possible to analyze the spatial structure itself. On the assumption that a large number of people move continually at random in the system, the ratio of elements in any row of the n-th power of the adjacency matrix means the ratio of people of all nodes in equilibrium. Mathematically, it is the ratio of eigenvector corresponding to the dominant (maximum) eigenvalue, Therefore it is the attribute immanent in the structure of spaces itself. Moreover, it is possible to consider the relative level of attraction of spaces and the relative flow from spaces to spaces only by putting the gravity on the adjacency matrix. The ERAM model brings forth the flexible result reflecting the relativity of spaces and flows.

재정정책과 민간소비 : 기간별 효용극대화 모형을 중심으로 In terms of Intertemporal Utility Maximization Models

김재필,최종수 순천향대학교 사회과학연구소 1997 순천향 사회과학연구 Vol.2 No.2

Since Ricardo's nineteenth-century suggestion that the means of financing government spending is irrelevant, theoretical and empirical debate concerning the burden of government debt has been vigorous. The substitution of debt for tax financing of a given rate of government expenditure would, according to the Neutrality hypothesis(NH), leave private-sector wealth, consumption decisions and consequently savings decisions unaltered. In this case, the implications for monetary and fiscal analysis would be far-reaching. In particular, the marginal net-wealth effect of government bonds is close to zero. The purpose of this paper is as follows. First, to examine the theoretical framework underlying the strict NH and various sources for deviations from the Neutrality(nonneutralities). Second, to review previous empirical work on the NH using intertemporal utility max models instead of the usual consumption function. Third, to derive Euler equation to be estimated directly from an explicit intertemporal utility max framework of the NH and to examine whether there is any substitutability between private consumer expenditure and government spending in Korea. Fourth, to test the NH. Both joint hypotheses are tested within the life-cycle/rational expectations model. Our framework results in a set of cross-restrictions implied by the theory of intertemporal optimization. On balence, we find no evidence of the NH in Korea since government deficits and spending impact on private-sector consumption through complementarity of public spending for private consumption in consumer's utility. These results tend to imply that temporary increases in government spending will result in an expansion of real output because private consumption is probably "crowded-in". But the results should be interpreted with some caution on two counts. First, this paper fails to test the alternative nonNeutrality hypothesis in its specification of the consumption. Second, considering a certain speciality for the monetary and fiscal policy such as the imperfect capital market, the fiscal rigidity, and rapid economic growth in Korea, our results are somewhat questionable. Finally, much more empirical work remains to be done on this crucial monetary and fiscal policy issue.

複合電力系統의 供給支障費推定을 위한 解析的方法의 開發

이순영,최재석,문승필 慶尙大學校生産技術硏究所 1997 生産技術硏究所論文集 Vol.13 No.-

This study proposes a new analytical method for assessing outage cost of composite power system with considering transmission system. Uncertainty of outages of generation system and transmission can be included. Composite power system ELDC(CMELDC) on HLII is developed and proposed newly from reliability evaluation of composite power system in order to analysis the outage cost on HLII. Equivalent outage cost of transmission system(EOCTS) is proposed newly in this study too. This means the outage cost conversed from unarrival which is power occurred by the outage of transmission system. The effectiveness of the proposed approach is demonstrated by the two case studies. The results show that this approach can evaluate the equivalent price of the outages of transmission lines.

말의 피부사상균증 및 피부 mycoflora에 관한 연구

최원필,이헌준,조길제,하태영,Choi, Won-pil,Lee, Hun-jun,Cho, Gil-jae,Ha, Tae-young 대한수의학회 1996 大韓獸醫學會誌 Vol.36 No.2

This study was carried out to determine the causative agent of dermatophytosis in 7 horses, and to examine the skin mycofloras on 84 healthy and 7 diseased horses which were derived from Jae-ju and Kyonggi, Korea in 1994~1995. Specimens of hair and scale were collected from skin lesions(or normal skins) and inoculated directly on potato dextrose agar and mycobiotic agar. These agar plates were incubated at $25^{\circ}C$ for 2 weeks. Growing fungi were isolated and identified by the morphological and nutritional characteristics. Lesions were found on the hind legs of an infected horses and each lesion was round or oval(1~4 cm) in shape accompanied by severe itching. The causative agent of the 7 equine dermatophytosis was identified as Trichophyton equinum. The skin mycofloras were Penicillium(69.0%), Aspergillus(63.2%), Cladosporium(51.7%), Fusarium(31.0%), Mucor(28.7%), Absidia(18.4%), Alternaria(17.2%), Acremonium(11.5%), Paecilomyces and Phycomyces(6.9%), Rhizopus(5.6%), Trichoderma(4.6%), Scopulariopsis and Trichophyton(3.5%), Beauveria(2.3%), Tritiracheum, Sporothrix, Curvularla, Aureobasidium and Chaetomium(1.2%), and Yeast(27.6%).