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한국해안으로부터 Purple, Non-Sulfur Photosynthetic Bacterium, Rhodobacter sp. EGH-24의 분리 및 특성
차미선,김기한,조순자,이나은,이정은,이재동,이상준,박재림 한국환경과학회 2003 한국환경과학회지 Vol.12 No.12
A species of facultative photo-organotrophic, purple, non-sulfur bacterium was isolated from the 47 point at west and south coast of Korea in September 2001. Separated 13 samples of changes with red color under 28~32 ℃, 3000 lux, anaerobe conditions for 7 days cultivated in basal medium. For pure isolation from 13 samples, we used agar-shake tube method (0.4 % agar) and separated 5 strains through 13-repetition test. EGH-24 and EGH-30 was identified as the same strain through the RAPD(Random Amplified Polymorphic DNA)-PCR of strain EGH-9, EGH-13, EGH-23, EGH-24, EGH-30. Four isolates cultivated in synthesis wastewater for wastewater biodegradation test. EGH-24 was selected with efficient wastwater treating strain. Based on the results obtained from morphology, nutrient requirements, major bacteriochlorophyll content, 16S-rDNA phylogenetic analysis, EGH-24 strain may be identified as a new strain of the genus Rhodobacter and named Rhodobacter sp. EGH-24.
Huge Abscess due to Mycobacterium Abscessus Infection after Breast Augmentation
SeungHwan Hwang,Sujin Bahk,Jae-Woo Chung,홍기용,SooA Lim,어수락 대한미용성형외과학회 2018 Archives of Aesthetic Plastic Surgery Vol.24 No.3
Periprosthetic capsular contracture, implant rupture, and deflation are well-known delayed complications of augmentation mammaplasty. However, infection remains the most common cause of reoperation after breast implant surgery. We report the case of a nontuberculous mycobacterial infection with huge abscess formation after augmentation mammaplasty. A 29-year-old woman visited our clinic with enlarged breasts after undergoing breast augmentation at a local clinic 4 years ago. She had no pain and tenderness, except some hardness around the breast margin. Nine months after surgery, her breasts began to grow larger, but showed no other typical symptoms, which led the patient to neglect the enlargement. After exploring through an inframammary approach, a large amount of serous fluid leaked out on both sides and we identified a huge abscess in a pocket localized in the submuscular plane. During exploration, the infected implants, which contained a pus-like fluid with a foul odor, were completely drained. The specimen culture revealed growth of Mycobacterium abscessus, which is a rare cause of infections after breast augmentation. While the overall incidence of mycobacterial infections after breast augmentation is low, our case demonstrates that huge abscess and granuloma formation should be considered as a potential complication of breast surgery.
Baek, Dong-won,Nam, Jae-sung,Koo, Yoon-Duck,Kim, Doh-Hoon,Lee, Ji-young,Jeong, Jae-Cheol,Kwak, Sang-Soo,Chung, Woo-Sik,Lim, Chae-Oh,Bahk, Jeong-Dong,Hong, Jong-Chan,Lee, Sang-Yeol,Maki Kawai-Yamada,Hi Plant molecular biology and biotechnology research 2004 Plant molecular biology and biotechnology research Vol.2004 No.-
An Arabidopsis protoplast system was developed for dissecting plant cell death in individual cells. Bax, a mammalian pro-apoptotic member of the Bcl-2 family, induces apoptotic-like cell death in Arabidopsis. Bax accumulation in Arabidopsis mesophyll protoplasts expressing murine Bax cDNA from a glucocorticoid-inducible promoter results in cytological characteristics of apoptosis, namely DNA fragmentation, increased vacuolation, and loss of plasma membrane integrity. In vivo targeting analysis monitored using jellyfish green fluorescent protein (GFP) reporter indicated full-length Bax was localized to the mitochondria, as it does in animal cells. Deletion of the carboxyl-terminal transmembrane domain of Bax completely abolished targeting to mitochondria. Bax expression was followed by reactive oxygen species (ROS) accumulation. Treatment of protoplasts with the antioxidant N-acetyl-L-cysteine (NAC) during induction of Bax expression strongly suppressed Bax-mediated ROS production and the cell death phenotype. However, some population of the ROS depleted cells still induced cell death, indicating that there is a process that Bax-mediated plant cell death is independent of ROS accumulation. Accordingly suppression of Bax-mediated plant cell death also takes place in two different processes. Over-expression of a key redox-regulator, Arabidopsis nucleoside diphosphate kinase 2 (AtNDPK2) down-regulated ROS accumulation and suppressed Bax-mediated cell death and transient expression of Arabidopsis Bax inhibitor-1 (AtBI-1) substantially suppressed Bax-induced cell death without altering cellular ROS level. Taken together. our results collectively suggest that the Bax-mediated cell death and its suppression in plants is mediated by ROS-dependent and -independent processes.
임준,이재문,김춘열,박용휘,Lim, Jun,Lee, Jae-Mun,Kim, Choon-Yul,Bahk, Yong-Whee 대한영상의학회 1986 대한영상의학회지 Vol.22 No.5
Since early 1980's high resolution CT has been used for detectionof intrathoracic condition such as superior vena cava syndrome. Authors retrospectively analysed CT findings of 18 cases of proven SVC syndrome. The results were as follows : 1. The mean age was 50-years-old, and 14 cases were male. 2. Of 18 cases of SVC syndrome, 8cases had confirmed to be lung cancers, malignant thymoma and teratoma were rewpectively each 2 cases, and malignant lymphoma, mediastinal abscess, thyroid adenoma and metastatic tumor were 1 case. 3. CT findings were A. Abnormal SVC consisted of compression with displacement (44.4%),intraluminal thrombus(27.8%), and encasement(27.8%). B. The collateral pathways were the azygos-hemiazygos(88.8%), vertebral(50%), internal mammary(44.4%), and lateral thoracic route(33.3%).
각성상태하에서 굴곡성내시경을 이용한 경비 및 경구 기관내 삽관시 혈압, 맥박, 심박축량의 변화
박진규,임영진,이국현,염광원,박재현 대한마취과학회 1991 Korean Journal of Anesthesiology Vol.24 No.3
To evaluate the clinical usefulness of fiberoptic intubation, we recorded the changes of arterial pressure, heart rate and cardiac output during fiberoptic nasotracheal and orotracheal intubation, while measuring the time taken for the intubation procedure. Anesthesia of pharynx was achieved by oral gargling of 10 ml of 4% lidocaine, and for nasotracheal intubation, nasal mucosa was anesthetized by application of 4% cocaine usirig cotton-tipped swabs. Anesthesia of the larynx and trachea was done by superior laryngeal nerve block with 6 ml of 2% lidocaine, and translaryngeal injection with 4% lidocaine by cricothyroid membrane puncture. After sedation with intravenous diazepam and fentanyl, awake fiberoptic (n=32) or orotracheal intubation (n=18) was performed on the patients (ASA class 1 or 2) in whom difficult intubation was expected. Mean arterial pressure, heart rate and cardiac output did not change significantly during intubation procedure, nor were these values significantly different between nasotracheal and orotracheal intubation group. The time taken by intubation procedure were compared, and there was no significant difference between two groups. In postoperative interviews, a few (3/28) patients complained discom-forts. It could be concluded that under approapriate anesthesia and sedation, awake fiberoptic nasotracheal or orotracheal intubation is a safe and useful approach producing minimal cardiovascular changes and discomforts.
소아에서 전신흡입 마취중 atropine 과 심박수의 용량-반응 관계
양미경,김성덕,임승운,박재현 대한마취과학회 1993 Korean Journal of Anesthesiology Vol.26 No.5
We determined the dose-response for atropine and heart rate in 114 healthy children anesthetized with N2O-O2-1 MAC halothane, enflurane or isoflurane. After induction of anesthesia, atmpine in does of 5, 10, 20 and 30 ug/kg was administered successively by rapid intravascular infusion with a time interval to each subject. The effects of atropine on heart rate and systolic blood pressure were compared among anesthetic groups and dosage groups, and a dose-response curve for peak heart rate was constructed in each anesthetic group. The heart rate increased in all cases following atropine medication. Among the dosage group of 5 ㎍/kg, the heart rate was significantly highest in children receiving isoflurane anesthesia (p$lt;0.05). Among 10 and 20 ㎍/kg, the heart rate was significantly lowest in enflurane group. In 30 ㎍/kg, the heart rate was not different in eaeh anesthetic group. In each dosage group, the systolic blood pressure was significantly lowest in isoflurane gorup (p$lt;0.05). In children anesthetized with halothane. 50% maximal response corresponded to 7.8 ㎍/kg, and 90% maximal response corresponded to 16.5 ㎍/kg. In enflurane group, 50% maximal response corresponded to 9.5 ㎍/kg, and 90% maximal response corresponded to 23.0 ㎍/kg. In isoflurane group, 50% maximal response corresponded to 7.5 ㎍/kg, and 90% maximal response corresponded to 18.3 ㎍/kg. Based on these data we conclude that 10 ㎍/kg of atopine is minimally required to increase heart rate by 50% of peak heart rate in children anesthetized with N2O-O2-1 MAC halothane, enflurane or isoflurane and that this also increases systolic blood pressure.
( Joo Hee Kim ),( Tae Wan Lim ),( Jae Hyon Bahk ) 대한마취과학회 2011 Korean Journal of Anesthesiology Vol.60 No.4
A 76-year-old, 148-cm woman was scheduled for right upper lobectomy. A 32 Fr left-sided double lumen tube was placed using a conventional technique. Despite several attempts under fiberoptic bronchoscope-guidance, we could not locate the double lumen tube properly. We thus decided to proceed with the bronchial tube in the right mainstem bronchus. During surgery, 8-cm-long laceration was noted on the posterolateral side of the trachea. To check the possibility of laceration of the proximal trachea, the double lumen tube was changed to an LMA for use as a conduit for fiberoptic bronchoscopic evaluation in the lateral position. A plain endotracheal tube with the cuff modified and collapsed was re-intubated after evaluation. And then she was transferred to SICU. (Korean J Anesthesiol 2011; 60: 285-289)
( Deok Man Hong ),( Yun Seok Jeon ),( Jun Hyun Kim ),( Tae Wan Lim ),( Young Jin Lim ),( Jae Hyon Bahk ),( Ki Bong Kim ),( Il Jae Kim ) 대한마취과학회 2010 Korean Journal of Anesthesiology Vol.58 No.2
Background: We hypothesized that, even in patients taking aspirin, the variance of preoperative platelet response to collagen might be associated with myocardial injury during coronary artery bypass graft (CABG) surgery. Therefore, we evaluated the relationship between preoperative whole-blood aggregometry (WBA) by collagen and the post operative myocardial injuries. Methods: For 44 patients who were scheduled for elective off-pump CABG and taking aspirin, WBA was measured by the impedance method in the presence of collagen (2 mg/ml or 5 mg/ml) as stimulatory agents. After CABG, myocardial injury was evaluated by analysis of the creatine kinase (CK), creatine kinase-MB (CK-MB), and lactate dehydrogenase (LD), and by electrocardiography. Results: High response group (n=13) on preoperative WBA with collagen (2 and 5 mg/ml) showed significantly higher postoperative cardiac enzyme levels (CK, CK-MB and LD) than those of low response group (n=31). Conclusions: In patients who take aspirin and undergoing off-pump CABG, the preoperative platelet response to collagen is correlated with postoperative myocardial injury. (Korean J Anesthesiol 2010; 58: 129-135)
Molecular and functional characterization of a PEX14 cDNA from rice
Lee, Jung-Ro,Lee, Kyun-Oh,Park, Jin-Ho,Yoo, Ji-Young,Kang, Jae-Sook,Jeon, Hye-Sook,Kim, Sun-Young,Lee, Young-Mi,Kim, Sun-Tae,Lim, Chae-Oh,Bahk, Jeong-Dong,Cho, Moo-Je,Lee, Sang-Yeol Plant molecular biology and biotechnology research 2003 Plant molecular biology and biotechnology research Vol.2003 No.-
In contrast to the translocation mechanisms determined in yeasts and mammalian cells, there is little information on the functions of plant peroxisomal proteins or their genomic structures. To understand the role that PEX14 plays in diverse plant peroxisomal functions and how peroxisomal translocation is mediated in plant cells, we cloned a 1827 bp cDNA encoding the peroxisomal membrane protein OsPex14p from a rice leaf cDNA library. The 54kDa OsPex14p, which has a theoretical pI value of 6.06, contains a highly conserved N-terminal domain and a short putative transmembrane domain. The OsPEX14 gene in the rice genome exists as a single-copy gene, consists of eleven exons interrupted by ten introns, and spans about 5kb of chromosome 5. The 5′-flanking region contains putative cis-acting light-responsive elements, and the transcription initiation site maps 114bp upstream of the translation start codon. OsPEX14 mRNA is highly expresssed in leaf tissues and is induced by exposure to several stresses. Heterologous expression of OsPex14p suppresses the defect in targeting of peroxisomal matrix proteins in a pex14 null mutant of Saccharomyces cerevisiae.