Baculovirus Vector System에 의해 발현된 재조합 Pseudorabies Virus Major Capsid Protein의 면역원성

전무형,안동준,장경수,조용성,박종현,송재영,현방훈,안수환 충남대학교 생물공학연구소 1997 생물공학연구지 Vol.5 No.-

The recombinant pseudorabies virus major capsid protein (rMCP) was produced by expression of the MCP gene in Sf-9 cell using baculovirus transfer vector system. Following evaluation of the immunochemical properties of the rMCP, the immunogenicity of the recombinant subunit protiens were investigated in guinea pig and swine to obtain the preliminary guide line for the subunit vaccine using rMCP and gP50. It was proved that ultrasonication and 30% ammonium sulfate was most efficient to concentrate and purify the protein. The rMCP was safe in mice, guinea pigs and piglets. In guinea pigs, rMCP mixed with various adjuvants induced substantial degree of serum neutralizing antibody titers, but revealed incomplete protecivity against challenge. In swine, the combination of rMCP and gP50 showed the higher serum neutralizing antibody titers and cellular immune responses than rMCP alone. However, the protectivity was lower in comparison with the commercial gI-deleted inactivated vaccine. We expect these results to contribute to characterization of MCP gene of Korean isolate of PRV and to ultilize as preliminary information for prodution and evaluation of PRV recombinant subunit vaccines.

안내염과 대장암이 동반된 재발성 간농양

전승정,김태헌,류민선,오다연,송명은,이신아,류재인,김혜인,문일환,유권 이화여자대학교 의과학연구소 2011 EMJ (Ewha medical journal) Vol.34 No.2

The causes of pyogenic liver abscess has been known as biliary tract disease or intrabadominal infection but the large proportions of the patients has no apparent underlying disorders. Recently colonic mucosal lesions were reported in patients with cryptogenic liver abscess and it has been suggested that colonic mucosal break may play a role in developing liver abscess in otherwise healthy patients. We experienced a patient of severe recurrent liver abscess complicated with endophthalmitis only 3 months after successful treatment of initial cryptogenic liver abscess and a polypoid colon cancer was discovered by chance. It seems prudent to proceed colonoscopic examination in patients with cryptogenic liver abscess especially when it is recurrent.

히스토아크릴을 이용한 십이지장 궤양 출혈의 지혈 후 발생한 급성췌장염

유재훈,문원,노지훈,구동영,조영화,김기수,윤준모 고신대학교 의과대학 2010 고신대학교 의과대학 학술지 Vol.25 No.1

Upper gastrointestinal bleeding is common and potentially life threatening medical emergency. Emergency endoscopy is the first choice of diagnostic and treatment measure for patients with active upper gastrointestinal bleeding. Histoacryl should be considered a second-line treatment modality when conventional treatments were failed. However, it might cause several complications. Herein, we report a case of acute pancreatitis developed after histoacryl injection therapy for active duodenal ulcer bleeding. A 71-year-old man was admitted with melena and hematemesis. On emergency endoscopy, a 2 cm sized active ulcer with bleeding from an exposedvessel was seen at the duodenal bulb. Attempts to arrest the bleeding with hemoclipping and submucosal epinephrine injection were tried, but failed. We changed the method to endoscopic histoacryl injection, and obtained hemostasis immediately. A few hourslater, after successful hemostasis, patient complained diffuse abdominal pain. Ultrasonography revealed hyperechoic heterogenous diffuse pancreatic enlargement and right pararenal space fluid collection, this ultrasonographic findings and elevated serum pancreatic enzymes are compatible with acute pancreatitis.

서울의 Penicillinase Producing Neisseria Gonorrhoeae 발생빈도(1996)

김재홍,황동규,전재홍,김윤석,김중환,김용준,이창균,임동진,김현수,조창근,김경문,박상훈,전우형,김희성,이호정,차명수,김갑형,김형석,김석우,황지환,박병순,권오상,이민수,송기훈,성소영,이인섭,부태성 대한화학요법학회 1999 대한화학요법학회지 Vol.17 No.2

Background : In recent years, gonorrhea has been panedemic and remains one of the most commom STDs in the world, especially in developing countries. Objective & Methods: For the detection of a more effective therapeutic regimen and assessing the prevalence of PPNG, we have been trying to study the patients who have visited the VD Clinic of Choong-Ku Public Health Center in Seoul since 1980 by means of the chromogenic cephalosporin method. Results: In 1996, 139 strains of N. gonorrhoeae were isolated, among which 53(39.0%) were PPNG. Conclusion: Our results suggests that after a peak of 74.3% in 1993, the prevalence of PPNG in Seoul is gradually declining.

이형성 신장과 동반된 이소성 요관

김재헌,한민철,박성재,정준호,심봉석,조민선 이화여자대학교 의과대학 2005 EMJ (Ewha medical journal) Vol.28 No.1

Ectopic ureteral orifice presents predominantly in young girls with duplicated kidneys and ureters, and mostly drains from ectopic ureters were associated with non-duplicated upper urinary systems and it is called a single system ectopic ureter. It is frequently associated with renal dysplasia or agenesis and ureterocele. Compared with ectopic ureter draining from the duplicated kidney, the diagnosis of single system ectopic ureter is often delayed because the ectopic ureter may be associated with a single small dysplastic, poorly functioning kidney. We report a case of single system ectopic ureter associated with dysplastic and poorly functioning kidney.

마우스의 BCG감염이 R.tsutsugamushi의 증식에 미치는 영향

김인재,전창덕,홍영권,정현택,이복수,장우현,김익상,박석돈 대한감염학회 1993 감염 Vol.25 No.2

It has recently been reported that gamma-inteferon(INF-r) and tumor necrosis factor(TNF) or lipopplysaccharide(LPS) activate macrophages to kill intracellular parasites by means of nitrc oxide (NO). It is now generally accepted that NO is the endothelium-derived relaxing factor, and that it also paticipates in the regulation of the nervous and immune systems. Activated macrophages form NO?? and NO?? from the terminal guanidino nitrogen atoms of L-arginine by the process now known to proceed via the formation of NO. This pathway is inhibited by the L-arginine analog(N?? monomethyl-L-arginine; N??MMA), forms L-citrulline as a co-product and is responsible for the cytotoxic action of macrophages. In this study, the authors found that INF-r and/or LPS induced murine macrophages to kill Rickettsiae tsutsugamushi in vitro and macrophages from BCG-infected mice got the host resistance against R.tsutsugamushi in vitro as well as in vivo. The rickettsicidal effect induced by the combination of INF-r and/or LPS induced mice got the host resistance against R.tsutsugamushi in vitro as well as in vivo. The rickettsicidal effect induced by the combination of INF-r and/or LPS can also be completely inhibited by N??MMA, leading to a dose-dependent inhibition of NO production. These datademonstrate that INF-r and/or LPS as well as BCG-activated macrophages mediate host resistance aganist R.tsutsugamushi infection through NO, which is necessary for the intracellular parasite.

Pseudorabies Virus의 Major Capsid Protein 유전자의 클론닝과 Baculovirus Vector System에 의한 발현

안동준,전무형,송재영,박종현,현방훈,장경수,안수환 충남대학교 생물공학연구소 1997 생물공학연구지 Vol.5 No.-

Pseudorabies is caused by Pseudorabies virus (PRV: Aujeszky's disease virus) of Herpesviridae that is characterized by 100 to 150nm in size with a linear double-stranded DNA molecule with of approximately 90 × 10 exp (6)Da. This disease affects most of domestic animals such as swine, cattle, dog, sheep, cat, chicken, etc. causing high mortality and economic losses. In swine, young piglets show high mortality and pregnant sows, reproductive failures. However the adult swine reveals no clinical signs in general. But they become a carrier state and play an importnat role for propagation of the disease. In this study, the nucleotide sequence of major casid protein gene of PRV, Yangsan strain isolated from the diseased swine in Korea was analyzed, and the recombinant MCP was produced by expression of the MCP gene in Sf-9 cell using baculovirus transfer vector system. As result, in BamHI digestion, MCP gene locus of PRV YS strain showed different from that of Indiana S strain. The patterns of enzyme mapping were also found to be unidentical each other. The sequence of the MCP gene partially analyzed showed 98.09% identity to Indiana S strain. The expression of MCP in Sf-9 cell cotransfected by pVLMCP-44 baculovirus expression vector was characterized by Southern blot hybridization, immunofluoresent and immunocytochemical tests, SDS-PAGE and Western blotting. The rMCP with M.W. 142kDa was most effectively expressed in Sf-9 cells at the 3-4th days post inoculation of the recombinant baculovirus by 2 moi.

우라늄 이온의 선택흡착

정종헌,김준수,오원진,유재형,유승곤 한국공업화학회 2002 응용화학 Vol.6 No.1

Uranium-containing aqueous wastes have been treated by electrosorption on a carbon electrode composed of activated carbon fibers(ACFs) in a continuous flow-through cell. Effective uranium (VI) removal is accomplished when a negative potential in the range of -0.3 to -0.9V (vs. Ag/AgCI) is applied to the carbon electrode. For a feed concentration of 350mg/l, the concentration of U(VI) in the cell effluent is reduced to less than lmg/l. Electrosorption capacity over 552 mg_uranium/g_ACF is reached.

서울의 Penicillinase Producing Neisseria Gonorrhoeae 발생빈도(1994)

김재홍,안진균,정성재,김영태,김중환,김시영,이석종,이홍렬,서호석,김경훈,권혁진,정우권,고우석,이용석,안필수,오준규,오용섭 대한화학요법학회 1996 대한화학요법학회지 Vol.14 No.1

Background : In recent years gonorrhoea has been panendemic and remains one of the most common Sexually Transmitted Diseases in the world, especially in developing countries. Objective & Method : For the detection of a more effective therapeutic regimen and assessing the prevalence of Penicillinase Producing Neisseria Gonorrhoeae(PPNG), Ive have been trying to study the patients who have visited the VD Clinic of Choong-Ku Public Health Center in Seoul since 1980 by means of the chromogenic cephalosporin method. Results : In 1994, 168 strains of N. gonorrhoeae were isolated, among which 109 (64,9% ) were PPNG. Conclustion : Our results suggests that the prevalence of PPNG in Seoul is still increasing.

大德 및 隣近地域 우물물의 tritium 濃度

李德憲,吳熙弼,田載植 충남대학교 자연과학연구소 1981 忠南科學硏究誌 Vol.8 No.2

Tritium concentrations in well water of Daedong area and its vicinity were measured by means of electrolytic enrichment technique and liquid scintillation counting method. Samples were taken from six different wells spread around Dae-deog Science Town at least ten kilometers and/or from each other, so as not to have any possible influence from other water sources and/or streams. The tritium enrichment factor in the electrolytic process was determined using standardized tritiated water of known activity. Results show that the mean value of tritium concentration in well water of this area is 41.60±2.52 TU with maximum and minimum of 44.33±2.64 TU and 39.20±2.65 TU, respectively, as of September 1981, which are quite reasonably comparable with 40 TU measured by Fireman and Schwarzer for well water.