저장용액의 온도에 따른 치주인대세포의 생존율

조재현,김성오,최형준,이제호,손흥규,최병재 대한소아치과학회 2007 大韓小兒齒科學會誌 Vol.34 No.1

외상성 손상 후 치아 탈구 시 치아를 저장하는 저장용액의 종류와 온도가 치주인대세포의 생존율에 어떠한 영향을 미치는지 연구하기 위하여 치주인대세포를 10% fetus bovine serum(FBD) 함유 α-minimal essential medium(α-MEM)에서 37℃ 5% CO_(2) 공기 혼합 배양기에서 배양하고 4.25, 37℃의 Hank's balanced sa1t solution(HBSS)과 α-MEM, 우유(S회사, P회사), tap water에 저장하고 60분이 지난 후 각 군에 대해 치주인대세포의 생존율을 측정하기 위하여 MTT assay를 시행하여 다음과 같은 결과를 얻었다. 1. 4℃ 저장용액의 치주인대세포의 생존율은 α-MEM과 P회사우유에서 가장 높았고 HBSS, S회사우유, tap water 순으로 낮았다. 2. 25℃ 저장용액의 치주인대세포의 생존율은 α-MEM에서 가장 높았고 P회사우유, HBSS, S회사우유, tap water 순으로 낮았다. 3. 37℃ 저장용액의 치주인대세포의 생존율은 α-MEM과 P회사우유, HDSS, S회사우유에서 높았고 tap water에서 가장 낮았다. 4. α-MEM의 치주인대세포의 생존율은 4℃에서 가장 높았고 25℃와 37℃ 순으로 낮았으며 HBSS에서는 4℃에서 높았고 25℃와 37℃에서 낮았다. 5. S회사와 P회사우유에서 치주인대세포의 생존율은 4℃와 25℃에서 높았고 37℃에서 낮았다. 이상의 결과를 종합해볼 때 외상으로 인해 치아가 탈구되었을 때 탈구된 치아의 저장용액으로 HBSS 용액이 추천되고 있으나 이 연구에서 4℃와 25℃ 우유에서 치주인대세포의 생존율이 높았으므로 사고 현장에서 쉽게 구할 수 있고 치주인대세포의 생활력 보존에도 유리한 낮은 온도의 우유에 탈구된 치아를 보존하는 방법도 좋을 것으로 생각된다. To compare the survival rate of periodontal ligament cells preserved in storage media with good availability at the time of an avulsion injury, periodontal ligament cells were incubated in α-MEM culture medium containing 10% FBS in condition of 37℃, 5% CO_(2). These cells were then cultured in HBSS, α-MEM, milk(S. co., P. co.) and tap water at the temperature of 4, 25, 37℃ each in 60 min. The groups were measured by MTT assay. The results were as follows : 1. Among the storage media at 4℃, α-MEM and P-milk had the highest preserving ability of periodontal ligament cells, while that of HBSS, S-milk and tap was low in order. 2. Among the storage media at 25℃, α-MEM had the highest preserving ability of periodontal ligament cells, while that of P-milk, HBSS, S-milk, tap water was low in order. 3. Among the storage media at 37℃, the preserving ability of periodontal ligament cells was very high in α-MEM, P-milk, HBSS and S-milk. it' s lowest in tap water. 4. The preserving ability of periodontal ligament cells in α-MEM was high at 4℃ and it’s low in order of 25℃, 37℃, but in HBSS was high at 4℃ and it's low at 25℃, 37℃. 5. The preserving ability of periodontal ligament cells in S-milk and P-milk was high at 4℃, 25℃ and it’s low at 37℃. In conclusion, HBSS is the storage medium of choice in an avulsion, but in this study it is preferable to choose milk at 4℃ for tooth since it is easy to get and affect cell viability.

人蔘多糖類의 理化學的 特性에 關한 硏究 : 人蔘貯藏加工中 澱粉의 理化學的 特性變化 Change of physicochemical properties of the starch during storage and heat treatment

曺哉銑,吳成基,曺羊嬉,金海中,黃明浩 경희대학교 부설 식량자원개발연구소 1985 硏究論文集 Vol.6 No.-

In order to investigate the change of physicochemical properties of ginseng root starch during storage and heat treatment. the roots were stored for 15 days at 5℃, 15℃, 30℃ and 45℃, and heated for 15 hours at 60℃, 70℃, 80℃, 90℃, respectively. The starch content was decreased from about 40% to 23-26% and sucrose content was increased from 4% to 12-16% during storage for 15 days at 5-45℃. Maltose, which was not detected in fresh samples, was increased upto 8.5% during storage or heat treatment. Granular size of the starch was decreased and some of the granules were broken during storage. Amylose content in the starch was decreased from 33% to 20%, and blue value and alkali number of the starch were increased slightly, and solubility and swelling power of the starch were decreased during storage. The higher storage temperature and the longer storage time, the starch was more susceptible to gelatinize, and the viscosity of the starch was lowered with the susceptibility of gelatinization. The susceptibility of degradation of the starch by the amylase was increased and amylolytic activities in ginseng root were, also, increased during storage.

고사리(Pteridium aquilinum)뿌리 전분의 이화학적 특성에 관한 연구 : 제2보 : 전분의 물리적 특성 Ⅱ. Physical Properties

曺哉銑,金成坤,李啓瑚,權泰完 경희대학교 부설 식량자원개발연구소 1981 硏究論文集 Vol.2 No.-

고사리뿌리 전분의 이화학적인 특성연구의 일환으로 실시된 물리적 특성시험 결과는 다음과 같다. 전분입자의 미구조의 결합력을 보기 위한 흡수도, 팽운력, 용해도 시험결과 감자전분이나 타피오카전분보다 강한 결합을 하고 있으며 곡류전분보다는 약하며 팽윤력이 single-stage pattern을 이루고 있었다. 가열에 의한 전분입자의 복굴절성 상실, α-아밀라아제에 의한 소화성 및 X-선 회절둥으로 전분의 호화과정을 살펴본 결과 55-60℃에서 호화가 시작되고 60~70℃에서는 95%이상 호화되었다. 또한 3~6%전분용액의 가열에 따른 점도변화를 amyl-ograph를 사용하여 실험한 결과 pasting temperature 62~68℃, 최고점도 80~840BU, 50℃로 냉각후의 점도는 110~555BU였다. 따라서 다른 전분에 비해서 점도가 낮으나 setback이 현저히 느렸다. 수분함량 50%의 전분겔의 경도는 팥 전분보다는 약하고 감자나 밀전분에 비해서는 현저히 높았다. 전분겔의 저장중 노화속도는 밀 전분보다는 느리고 파지오카 전분보다는 빨랐다. 고사리뿌리 전분은 팥 전분겔특성에 큰 변화를 주지않지만 노화를 억제하였고 밀 전분겔에 첨가시에는 겔의 경도가 저하되어 부드러워지는 경향을 보였다. The gelatinization phenomena of bracken root starch were examined by means of the loss of birefringence, degree of digestibility by amylase and X-ray diffraction. These results indicated that gelatinization temperature of the starch was 55∼60℃ and over 95% of starch were gelatinized at the temperature between 60 and 70℃. The swelling power of the bracken root starch was much less steeper than that of potato or tapioca starch. Amylograph data on the various starch concentrations showed the pasting temperature of 62∼68℃, peak height of 80∼840 Brabender unit (BU) and peak after cooling to 50℃ of 110∼555 BU. According to the information obtained from amylograph data, the bracken root starch showed low set back. The rate of retrogradation of the starch as tested by Texturometer was slower and faster than potato and tapioca starches, respectively.

Engineering Art 기반 작곡 및 동영상 편집

조재영,김윤호 목원대학교 멀티미디어신기술연구소 2002 멀티미디어신기술연구소논문집 Vol.2 No.1

본 연구에서는 S/W 기반 음악작곡법과 작곡된 음악을 기반으로 한 동영상 편집기술에 대해 기술하였다. 이번 컨텐츠의 결과물은 미디로 작곡된 음악에 동영상관련 S/W를 활용한 동영상을 조합한 것으로, 누구나가 쉽게 접근할 수 있는 컴퓨터음악 작곡과 영상편집에 대한 배양이 주제이자 목표이다. In this paper, the scheme of music composition which is based on s/w system, and the skill of moving image edit is presented. The result of this contents informs the mixture of a composed music by the Midi program and a moving image for using by the s/w system, also guides many students that the computer music composition and image edit is not a difficult sphere. Experimental results showed that more effective approach than traditional method for music composition and image edit.

심첨부 비후 심근증에서 심근 내 미세 관상동맥의 혈류 예비력 평가 : 경흉부 도플러 심초음파를 이용한 연구 Study Using Transthoracic Doppler Echocardiography

윤호중,박철수,신재원,임상현,조은주,정해억,전희경,오용석,정욱성,김재형,최규보,홍순조 대한순환기학회 2004 Korean Circulation Journal Vol.34 No.3

전격성 경과를 취한 만성 호산구성 폐렴 환자 1예

윤호상,진춘조,유광하,이상엽,이수인,정상만,김선두,이순제,이길도,전혜정 건국대학교 의과학연구소 2000 건국의과학학술지 Vol.10 No.-

Chronic eosinophilic pneumonia(CEP) is a rare disease of unknown etiology characterized by chronic infiltration of the lung with eosinophils. It presents with profound systemic symptoms comprising fever, night sweats, weight loss, dyspnea and blood eosinophilia with nonsegmental air-space consolidation confined to the outer third of the lung, the "photographic negative of pulmonary edema". Histopathologic features of chronic eosinophilic pneumonia are intraalveolar and interstitial infiltrations with eosinophils, histiocytes, giant cells, scattered lymphocytes and plasma cells. Glucocorticoid therapy cause prompt resolution of symptoms as well as disappearans of blood eosinophils, elevated serum Ig E levels and the roentgenographic lesions. Infrequent radiographic findings include nodular infiltrates, consolidations, cavitations, atelectasis and pleural effusions. Deaths from CEP although rare, have been reported, but the majorities of CEP have benign courses and do not need a therapy with ventilator. We report a case of chronic eosinophilic pneumonia, which had a sudden course associated with diffuse pneumonic consolidations on the both lung and bilateral pleural effusion.

우모 분해균의 분리 및 특성에 관한 연구

강호조,이후장,정태성,김도경,어용준,김재황 한국수의공중보건학회 2003 예방수의학회지 Vol.27 No.3

Feather waste, generated in large quantities as a byproduct of commercial poultry processing, is almost pure keratin, which is not easily degradable by common proteolytic enzymes. Several feather-degrading bacterial isolates were isolated from feather waste and soil. These isolates were able to degrade chicken feather, when grown on basal medium containing 1.0% native feather as a source of energy, carbon, and nitrogen. The isolates were characterized according to morphological characteristics and biochemical tests, and Vitek and API kit system. belonging to the Acinetobacter calcoaceticus complex, Bacillus thuringiensis, Bacillus circulans, Bacillus cereus. It was observed that aerobic growth by the isolate on single whole chicken feather, resulted in complete degradation of the keratin protein after 5-6 days of incubation at 37℃. The production of protein by the organism was decreased with cultivation time, with the maximum activity coinciding with the start of the stationary phase. The kerationolytic activity increased with cultivation time.

사용후 핵연료 처리공정물질의 핵물질 비파괴분석을 위한 중성자 증배 보정인자에 관한 연구

김현조,박재우,고원일,김호동 제주대학교 방사능이용연구소 2002 연구보고 Vol.16 No.-

The quantity of uranium or plutonium present in bulk samples of metal, oxide, mixed-oxide, fuel rods, etc., can often be assayed nondestructively by means of neutron coincidence counting. The self-multiplication phenomenon, which results from the induced fissions existing within the sample, inevitably generates measurement error. In this study, neutron multiplication effects were investigated by the measurement method proposed by Ensslin, and then the correction curves of neutron multiplication were derived. For this, DSNC(DUPIC Safeguards Neutron Counter), a well-type neutron coincidence counter, was use. In addition, the neutron multiplication effects were verified by comparison with the result of MCNP code. The correction curve obtained from this study will be used to increase measurement accuracy of the well-type neutron accounting sysyem in the future.

유산균(L.lactis CBT-8)및 발효 추출물의 Helicobacter pylori 억제 능력

진춘조,박형석,이혜운,김성렬,강동구,이준상,이재동,왕준호 건국대학교 의과학연구소 2002 건국의과학학술지 Vol.12 No.-

Helicobacter pylori infection was the cause of chronic gastritis, duodenal ulcer and gastric MALT lymphoma. The eradication rates of H. pylori using antibiotics are around 80%. Lactobacilli have been demonstrated to have in vivo and in vitro inhibitory effects on H. pylori infection. We aimed to evaluate the inhibitory effect of L. lactis CBT-8 and its extract on H. pylori in human stomach. Thirty-five H. pylori-infected volunteers(30-49 yr)were randomized into two groups which were treated with L. lactis CBT-8 and its extract(Group A n=17) or milk containing L. lactis CBT-8(Group B, n=18). They underwent 13C-urea breath tests before and after treatment. The DOB30 of group A(n=14) was decreased 41.0% after treatment(26.0±4.9 before, 15.3±2.1 after treatment)(p<0.05). the DOB30 of group B(n=13) was decreased 22.3% after treatment(22.0±2.2 before, 17.1±1.7 after treatment)(p<0.05). In conclusion, L. lactis CBT-8 was effective in suppressing H. pylori infection in human stomach.

제5차 고등학교 물리교과서의 분석적 연구

박재호,김학수,이영주,조환옥 부산대학교 사범대학 과학교육연구소 1990 科學敎育硏究報 Vol.17 No.-

The rivised physics textbook is going to used in class starting 1991. the content of Unit Ⅰ, which is introduced in the physics test book for the first time, and the formats of the text books of 7 publishers and studied. The contents of the newly introduced Unit Ⅰ shows varieties among the publishers, while other Units of the texts in 7 different text book show no much differences form each other.