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Lee, Moon Hee,Cha, Hee-Jae,Choi, Eun Ok,Han, Min Ho,Kim, Sung Ok,Kim, Gi-Young,Hong, Su Hyun,Park, Cheol,Moon, Sung-Kwon,Jeong, Soon-Jeong,Jeong, Moon-Jin,Kim, Wun-Jae,Choi, Yung Hyun Spandidos Publications 2017 International journal of molecular medicine Vol.39 No.3
<P>Natural phytochemicals of plant origin, including flavonoids, have been found to be potent antioxidants providing beneficial effects against oxidative stress-related diseases. The present study was carried out to investigate the antioxidant properties of morin, a flavonoid originally isolated from the flowering plants of the Moraceae family. Superoxide dismutase (SOD)-like activity and 2,2'-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS(center dot+)) radical scavenging activity were determined. We also investigated the cytoprotective effects of morin against hydrogen peroxide (H2O2)-induced DNA damage and apoptosis in V79-4 Chinese hamster lung fibroblasts. Our results demonstrated that morin had strong scavenging effects against ABTS' radicals with enhanced SOD activity, which varied in a dose-dependent manner. Morin was found to reduce H2O2-induced intracellular reactive oxygen species generation and nuclear DNA damage, and it recovered cell viability damaged by H2O2 via inhibition of mitochondrial dysfunction-mediated apoptosis. Notably, the treatment of V79-4 cells with morin markedly enhanced the expression of heme oxygenase-1 (HO-1) but not quinone oxidoreductase-1, which was associated with the increased expression and phosphorylation of nuclear factor-erythroid 2-related factor 2 (Nrf2) and the downregulation of Kelch-like ECH-associated protein 1 expression. Based on our findings, we conclude that morin effectively ameliorated oxidative stress-induced DNA damage through intrinsic free radical scavenging activity and activation of the Nrf2/HO-1 pathway.</P>
Lee, Moon Hee,Han, Min Ho,Lee, Dae-Sung,Park, Cheol,Hong, Su-Hyun,Kim, Gi-Young,Hong, Sang Hoon,Song, Kyoung Seob,Choi, Il-Whan,Cha, Hee-Jae,Choi, Yung Hyun UNKNOWN 2017 INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE Vol.39 No.2
<P>In the present study, we investigated the cytoprotective efficacy of morin, a natural flavonoid, against oxidative stress and elucidated the underlying mechanisms in C2C12 myoblasts. Our results indicated that morin treatment prior to hydrogen peroxide (H2O2) exposure significantly increased cell viability and prevented the generation of reactive oxygen species. H2O2-induced comet-like DNA formation and gamma H2AX phosphorylation were also markedly suppressed by morin with a parallel inhibition of apoptosis in C2C12 myoblasts, suggesting that morin prevented H2O2-induced cellular DNA damage. Furthermore, morin markedly enhanced the expression of heme oxygenase-1 (HO-1) associated with the induction and phosphorylation of nuclear factor-erythroid 2-related factor 2 (Nrf2) and the inhibition of Kelch-like ECH-associated protein 1 (Keapl) expression. Notably, these events were eliminated by transient transfection with Nrf2-specific small interfering RNA. Additional experiments demonstrated that the activation of the Nrf2/HO-1 pathway by morin was mediated by the extracellular signal-regulated kinase (ERK) signaling cascade. This phenomenon was confirmed with suppressed Nrf2 phosphorylation and consequently diminished HO-1 expression in cells treated with a pharmacological inhibitor of ERK. Collectively, these results demonstrated that morin augments the cellular antioxidant defense capacity through the activation of Nrf2/HO-1 signaling, which involves the activation of the ERK pathway, thereby protecting C2C12 myoblasts from H2O2,-induced oxidative cytotoxicity.</P>
간흡충 重複感染이 실험동물의 膽管에 미치는 병리조직학적 변화에 관한 연구
車聖昊,李在賢,林漢鍾 고려대학교 의과대학 1991 고려대 의대 잡지 Vol.28 No.3
The study was carried out to investigate the distingate the changes of the bile duct of the rats. the mice and the rabbits by the superinfection of Clonorchis sinensis. Experimental animals were devided into two larged groups. the single-infection groups and the superinfection groups. In single-infection groups. 40 rats and 40 mice were infected with 10 (Rt-Ⅰ group. Mo-Ⅰ group) and 50 (Rt-Ⅱ. Mo-Ⅱ) metacercaria to each animal respectively and 30 rabbits with 100 (Rb-Ⅰ) and 500 (Rb-Ⅱ) metacercaria. For the superinfection groups. the other 60 rats and 60 mice each were infected twice (Rt-Ⅲ. Mo-Ⅲ). three times (Rt-Ⅳ. Mo-Ⅳ), four times (Rt-Ⅴ. Mo-Ⅴ) and five times (Rt-Ⅵ, Mo-Ⅵ) in every 2 weeks with 10 metacercaria to each rat and mouse respectively and the other 20 rabbits each were given three times (Rb.Ⅲ) or five times (Rb-Ⅳ) in every 2 weeks with 100 metacercaria. In order to observe the histopathological changes of the bile ducts in these animals. 2 to 3 animals of each group were sacrificed in every 2 weeks. The result were as follows. In rats superinfected with C. sinensis, a lots of eosinophils was infiltrated around the bile duct after 2 to 4 weeks of each infestation of C. sinensis and after that the cells were reduced and replaced by massive mononuc1ear cells forming the follicles. In addition to these. Russell bodies the alteration of plasma cell were increased around the bile duct as the superinfection of C. sinensis. In the bile ducts of mice superinfected with C. sinensis. there were no any difference with those of the single-infected mice however the histopathological changes of the bile duct showed particular findings in comparison to the other animals. The epithelial cells of the bile duct were changed to secretary cells and these cells secreted the hyalinized materials into the lumen of vile duct (hyaline change). The inflammatory cells were infiltrated in the parenchyma of the liver and formed the microabscess. In rabbits, the preiductal fibrosis was progressive as the period of the infection and/or the superinfection of C. sinensis.
차재호 서울大學校 社會科學大學 心理科學硏究所 1994 心理科學 Vol.3 No.1
구한말(1860년경)이래 70년대까지의 한국인에 관한 외국인 견문록들과 60년대 이래의 가치관 조사 연구들을 토대로 80년대까지의 약 100년간에 걸친 한국사회의 가치관 변화를 개관하고 이를 토대로 가치의 성질, 가치의 생성과 변화, 그리고 한국사회에서의 가치연구방법에 관한 27개의 명제를 제시했다. The paper summarized the value changes that occurred within the Korean society in about 100 years from 1860's through 1980's as determined through an analysis of foreigner's travelogues and empirical studies of values. Based on the findings, a set of 27 hypotheses were proposed relating to the nature and functions of value, generation and change of value, method of investigation, and value changes specific to Korea.