DNA 증거의 집단유전학적 분석을 위한 SAS시스템

이효정,이혜승,한길로,이재원,황적준 大韓法醫學會 2000 대한법의학회지 Vol.24 No.1

DNA analysis has become one of the most powerful tools in forensic inference for human identification and is now used worldwide. It is used to be statistical technique for the individual identification of a civil and criminal action. The purpose of this article is computerization of the statistical technique for the population study and DNA evidence analysis. The system using SAS/AF and SAS/SCL is the graphic user interface and the correspondence of the changed experimental circumstances

A Calculation Model to Assess Two Irreversible Capacities Evolved in Silicon Negative Electrodes

Lee, Jae Gil,Kim, Jongjung,Park, Hosang,Lee, Jeong Beom,Ryu, Ji Heon,Kim, Jae Jeong,Oh, Seung M. The Electrochemical Society 2015 Journal of the Electrochemical Society Vol.162 No.8

<P>A novel calculation model is devised to quantitatively assess two irreversible capacities evolved in Si negative electrodes: electrolyte decomposition and Li trapping. In this model, the capacity of the electrode reaction (Li-Si alloy formation, Q<SUP>n</SUP><SUB>alloy</SUB>), which is the only implicit value on the galvanostatic charge/discharge voltage profiles, is calculated with the data obtained from GITT (galvanostatic intermittent titration technique) experiment. When the calculation model is applied to two Si electrodes of different particle sizes, the particle size is found to significantly affect the nature of the irreversible reactions. In the bulk-sized Si electrode, Li trapping is dominant over electrolyte decomposition. This feature must be due to an electrical contact loss that is caused by crack formation, which is more vulnerable to bulk-sized Si particles. The hump behavior in the Coulombic efficiency profiles is also explained by the Li trapping. In the nano-sized Si electrode, electrolyte decomposition is the major irreversible reaction because of its larger surface area. Because of a stronger endurance against mechanical stress, crack formation and subsequently Li trapping are less severe than that of the bulk-sized one.</P>

Mechanical Damage of Surface Films and Failure of Nano-Sized Silicon Electrodes in Lithium Ion Batteries

Lee, Jae Gil,Kim, Jongjung,Lee, Jeong Beom,Park, Hosang,Kim, Hyun-Seung,Ryu, Ji Heon,Jung, Dong Sub,Kim, Eun Kyung,Oh, Seung M. The Electrochemical Society 2017 Journal of the Electrochemical Society Vol.164 No.1

<P>This work demonstrates that the mechanical damage of surface passivation films plays an underlying role in the failure of nano-sized Si electrodes in lithium-ion batteries. The surface film derived from the standard electrolyte (1.3 M LiPF6 dissolved in ethylene carbonate/diethyl carbonate) during the first lithiation step is damaged by the mechanical stress caused by the volume contraction of Si particles during the subsequent de-lithiation period. The electrolyte decomposes on the newly exposed Si surface and film deposition occurs, which is then mechanically damaged again owing to volume change of the Si particles. Such film deposition/damage cycles are repeated until the mechanical stress becomes insignificant as a result of capacity decay. Continued electrolyte decomposition, which prevails in the early cycling period, produces electronically insulating films located between Si particles, which cause Li trapping within the Si matrix. Li trapping is found to be responsible for the rapid decrease in capacity and Coulombic efficiency in the intermediate period of cycling. When fluoroethylene carbonate (FEC) is added to the electrolyte, a surface film that is robust against mechanical stress is produced. As a result, the FEC-derived surface film maintains its passivating ability and suppresses the irreversible reactions, resulting in a better cycling performance. (C) The Author(s) 2016. Published by ECS. This is an open access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives 4.0 License (CC BY-NC-ND, http://creativecommons.org/licenses/by-nc-nd/4.0/), which permits non-commercial reuse, distribution, and reproduction in any medium, provided the original work is not changed in any way and is properly cited. For permission for commercial reuse, please email: oa@electrochem.org. All rights reserved.</P>

만성신부전증환자에서 부갑상선 절제술 시 수술 중 급속 부갑상선 호르몬 측정의 의의

김우영,손길수,배정원,구범환,이재복,Woo Young Kim,M,D,Gil Soo Son,M,D,Jeoung Won Bae,M,D,Bum Hwan Koo,M,D,and Jae Bok Lee,M,D 대한갑상선-내분비외과학회 2005 The Koreran journal of Endocrine Surgery Vol.5 No.2

Purpose: Intraoperative quick parathyroid hormone assay (PTH) was introduced in the parathyroid surgery since 1988 and the value in patients with primary hyperparathyroidism was well recognized in the literature. The purpose of this study was to evaluate the usefulness of intraoperative rapid PTH assay in patients with renal hyperparathyroidism by comparing intraoperative PTH results and the biochemical results at postoperative 6<SUP>th</SUP> month, including PTH values. Methods: Fifteen consecutive patients of renal hyperpara</SUP>thyroidism underwent total parathyroidectomy and immediate autotransplantation from November 2003 to February 2005. PTH levels were measured by PTH assay at the induction of anesthesia (baseline level) and in 20-minute intervals after excision of the last parathyroid gland. More than 50% drop of initial PTH level was considered as completeness of parathyroidectomy. Results: Twenty minutes after resection, PTH levels decreased by 83.7% in 14 patients and by 50.2% in one patient. Ten patients (67%) were cured but 5 patients (34%) showed high PTH levels after 6 months. The drop rate of intraoperative quick PTH level in cured patients was 92% at 26 minutes after parathyroidectomy and was significantly different from 73.3% of persistent or recurrent five patients (P=0.047). Preoperative PTH level, calcium level, alkaline phosphatase level and preoperative localization were not different in the cured and recurrent or persistent patients of renal hyperparathyroidism. Conclusion: The value of intraoperative quick PTH assayin patients of renal hyperparathyroidism was questionable. More than 92% drop of intraoperative PTH level at 26 minutes after parathyroidectomy could predict success in our study. (Korean J Endocrine Surg 2005;5:93-99)

갑상선 유두암에서의 bcl-2와 P53 단백질 발현의 의의

지웅배,배정원,우상욱,손길수,이재복,구범환,채양석<SUP>1<,SUP>,Woong Bae Ji,M,D,Jeoung Won Bae,M,D,Sang Uk Woo,M,D,Gil Soo Son,M,D,Jae Bok Lee,M,D,Bum Whan Koo,M,D,and Yang-Seok Chae,M,D,<SUP>1<,SUP> 대한갑상선-내분비외과학회 2007 The Koreran journal of Endocrine Surgery Vol.7 No.4

Purpose: Papillary thyroid cancer (PTC) has a good prognosis, and it's known to be related to the apoptosis of papillary thyroid cancer. The expression of bcl-2 is thought to be associated with the inhibition of apoptosis. We evaluated the differences of bcl-2 and P53 between PTC and the control (normal tissue and benign lesion). We then analyzed the correlation between the bcl-2 and P53 expressions and the classic prognostic factors. Methods: Between January 2001 and December 2005, 30 patients who underwent total thyroidectomy for the PTC were included in this study and immunohistochemical staining was performed on the tumors. Results: bcl-2 was expressed in 18 cases (60%) in their PTC (P<0.05). The expression of P53 was not significantly related with the clinicopathological factors, but P53 was expressed in 9 cases (30%) of PTC (P<0.05). The positive staining for was noted in 18 cases (62.1%) of the PTC tissue among the 30 patients, and as the TNM stage progresses, the expression rate of was significantly decrease for 7 stage I cases (100%), for 4 stage ll cases (80%) and for 7 stage ll cases (38.9%). Conclusion: bcl-2 was expressed more as the TNM stage of PTC decreases. So bcl-2 is possibly useful as a prognostic factor for PTC, but further studies are needed for confirming its significance. (Korean J Endocrine Surg 2007; 7:231-236)

Thermal Degradation of Solid Electrolyte Interphase (SEI) Layers by Phosphorus Pentafluoride (PF₅) Attack

Kim, Jongjung,Lee, Jae Gil,Kim, Hyun-seung,Lee, Tae Jin,Park, Hosang,Ryu, Ji Heon,Oh, Seung M. The Electrochemical Society 2017 Journal of the Electrochemical Society Vol.164 No.12

<P>Thermal degradation mechanisms of solid electrolyte interphases (SEIs) on graphite and SiO electrodes are examined at moderately elevated temperatures (60-130 degrees C). Of the two possible degradationmechanisms, the attack of phosphorus pentafluoride (PF5), which is generated by thermal decomposition of lithium hexafluorophosphate (LiPF6) used as the lithium salt, dominates over the thermal decomposition of the SEI layer itself over this temperature range. Once the SEI layer is damaged, electrolyte decomposition and film deposition takes place on the newly exposed electrode surfaces due to the loss of passivating ability; this is a repair process for the damaged SEI. Such damage/repair of the SEI layer continues until the Li+ ions/electrons are exhausted from the negative electrodes. An undesired feature of this process is the steady increase in SEI thickness, which causes electrode polarization and eventual capacity fading. (C) 2017 The Electrochemical Society. All rights reserved.</P>

Artificially-built solid electrolyte interphase via surface-bonded vinylene carbonate derivative on graphite by molecular layer deposition

Chae, Seulki,Lee, Jeong Beom,Lee, Jae Gil,Lee, Tae-jin,Soon, Jiyong,Ryu, Ji Heon,Lee, Jin Seok,Oh, Seung M. Elsevier 2017 Journal of Power Sources Vol.370 No.-

<P><B>Abstract</B></P> <P>Vinylene carbonate (VC) is attached in a ring-opened form on a graphite surface by molecular layer deposition (MLD) method, and its role as a solid electrolyte interphase (SEI) former is studied. When VC is added into the electrolyte solution of a graphite/LiNi<SUB>0.5</SUB>Mn<SUB>1.5</SUB>O<SUB>4</SUB> (LNMO) full-cell, it is reductively decomposed to form an effective SEI on the graphite electrode. However, VC in the electrolyte solution has serious adverse effects due to its poor stability against electrochemical oxidation on the LNMO positive electrode. A excessive acid generation as a result of VC oxidation is observed, causing metal dissolution from the LNMO electrode. The dissolved metal ions are plated on the graphite electrode to destroy the SEI layer, eventually causing serious capacity fading and poor Coulombic efficiency. The VC derivative on the graphite surface also forms an effective SEI layer on the graphite negative electrode via reductive decomposition. The detrimental effects on the LNMO positive electrode, however, can be avoided because the bonded VC derivative on the graphite surface cannot move to the LNMO electrode. Consequently, the graphite/LNMO full-cell fabricated with the VC-attached graphite outperforms the cells without VC or with VC in the electrolyte, in terms of Coulombic efficiency and capacity retention.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Ring-opened VC is chemically bonded on graphite surface through MLD method. </LI> <LI> The attached VC copies reduction behavior of VC as additives generating SEI via C=C. </LI> <LI> VC-attached graphite outperforms the pristine graphite in both half cell and full cell. </LI> <LI> VC molecules added in electrolyte generate acid when oxidized. </LI> <LI> The acid generated by VC oxidation accelerates transition metal dissolution from LNMO. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

단백질 전달체로서 천연 및 합성재료의 첨가에 따른 PLGA와 PCL 웨이퍼로부터 알부민의 방출거동

현훈(Hoon Hyun),이재호(Jae Ho Lee),서광수(Kwang Su Seo),김문석(Moon Suk Kim),이종문(Jhon M. Rhee),이해방(Hai Bang Lee),강길선(Gil Son Khang) 한국고분자학회 2005 폴리머 Vol.29 No.5

Development of Biodegradable Methoxy Poly(ethyleneglycol)-polyesters Diblock Copolymers as Drug Carrier for Implantable Disc

( Jong Tae Ko ),( Jae Ho Lee ),( Jong Min Kim ),( Moon Suk Kim ),( John M. Rhee ),( Hai Bang Lee ),( Gil Son Khang ) 한국조직공학과 재생의학회 2006 조직공학과 재생의학 Vol.3 No.2

The main objective of this study is to investigate the feasibility of methoxy polyethyleneglycol (mPEG) and poly(D,L-lactide-co-glycolide) (PLGA) or poly(ε-caprolactone) (PCL) diblock copolymers as implantable drug carriers. A series of diblock copolymers were synthesized by ring-opening polymerization with different molecular weight of mPEG. 1H-NMR, FT-IR, XRD and GPC were used to characterize the obtained various diblock copolymers. The fabricated dexamethasone-loaded discs were incubated at 37 oC in phosphate buffer saline (PBS, pH 7.4) and the effects of composition on the degradation rate of discs were examined by water absorption, mass loss, pH variation of in vitro media and composition changes using SEM, FT-IR, respectively. The in vitro rate of drug-loaded mPEG-PLGA, mPEG-PCL implantable discs depended on their composition, increasing when the mPEG molecular weight of diblock copolymers increased. Water absorption and mass loss of mPEG-PLGA copolymer discs as drug carrier seems to be more affected on mPEG molecular weight than mPEG-PCL copolymers. Also, the variation of pH of PBS as in vitro media was changed by cleavage of ester bond by hydrolysis. These results indicate that pH of PBS was faster decreased with increasing of mPEG molecular weight in the all copolymers. It can be expected that optimized copolymer composition and molecular weight may be providing further application as the implantable drug carrier for different drugs in controlled drug delivery system.

Low-Temperature Characteristics and Film-Forming Mechanism of Elemental Sulfur Additive on Graphite Negative Electrode

Jurng, Sunhyung,Kim, Hyun-seung,Lee, Jae Gil,Ryu, Ji Heon,Oh, Seung M. The Electrochemical Society 2016 Journal of the Electrochemical Society Vol.163 No.2

<P>We report here that the low-temperature performance of graphite electrode is improved by adding a small amount of elemental sulfur (S-8) into the graphite negative electrode. The reversible capacity at -30 degrees C is much larger for the sulfur-added graphite electrode. The origin of this beneficial feature is examined through impedance analysis, which illustrates that the charge transfer resistance is much smaller in the sulfur-added graphite electrode at low temperatures. In the first lithiation step, the elemental sulfur is electrochemically reduced to be lithium polysulfide (Li2S8), which is soluble in the working solvent (carbonate-based ones). Organic thiocarbonates are generated by the chemical reaction between the lithium polysulfide and carbonate solvents. The as-generated thiocarbonates are then electrochemically decomposed to form the sulfur-containing surface film. The superior low-temperature performance of the sulfur-added graphite is thus attributed to the presence of sulfur-enriched surface film, which seems to facilitate the charge transfer reaction between the graphite and lithium. (C) The Author(s) 2015. Published by ECS.</P>