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Koo, Ja-Choon,Chun, Hyun-Jin,Lee, Bok-Luel,Cho, Moo-Je Plant Molecular Biology & Biotechnology Research C 1994 Plant molecular biology and biotechnology research Vol.1994 No.
Two antifungal proteins with low molecular weight (approx. 4kD) have been purified from seed of P.nil and amino acid sequence of one of them determined. To isolate cDNA encoding the antifungal protein, degenerated oligonucleotides corresponding to the peptide sequence of the antifungal protein and oligo (dT)_(18) were used to PCT primer. Several positive cDNA clones were isolated and the nucleotide sequence of one cline having the longest insert, designated as Pn-AFP1, was determined. Pn-AFP1 consisted of three domains; a 25 amino acids putative N-terminal signal peptide, the 39 amino acids mature protein and a 28 amino acids C-terminal propeptide. Pn-AFP1 hybridized to 0.8 and 0.45kb ClaI fragment as well as 4.5 and 3.5kb XbaI fragment of P.nil genomic DNA. Moreover, each two bands from ClaI or XbaI digestion showed different degree of hybridizing signals with cDNA probe. This result indicate that p.nil genome contains two copies of Pn-AFP.
Representation of a Conceptual Design for a Rectilinear Motion Polymer Actuator
Ja Choon Koo,Kwang Mok Jung,Jong Kil Park,Jae Do Nam,Young Kwan Lee,Jae Wook Jeon,Hyouk Ryeol Choi 대한전기학회 2007 International Journal of Control, Automation, and Vol.5 No.4
A number of different alternative actuation methods have been under active development for some specific applications where the traditional electromechanical actuators are difficult to apply. Recently, many of these substitutes are trying to employ new smart materials like electroactive polymers. However most of the polymeric materials are flexible and vulnerable so that they normally can not sustain external forces. Although the materials have shown a good potential to be used for alternative actuation mechanisms, no tangible industrial application is yet presented because of the reason. A conceptual design for a rectilinear motion actuator using dielectric elastomer is presented in this article. The introduced design concept might enable to produce fairly controllable rectilinear motions for various applications and the presented prototype actuator system is fully packaged in a small unit and controlled by a standard communication interface.
( Ja Choon Koo ),( Yeo Chang Youn ),( Mi Sun Park ),( Myeong Eun Kim ) 한국임학회 2012 한국임학회 학술발표논문집 Vol.2012 No.-
South Korea is not included in Annex I countries under the Kyoto protocol. However, its greenhouse gas (GHG) emissions were listed 9th highest in the world. South Korean government announced a goal of reducing GHG emissions by 30 percent compared to the business as usual by 2020. In order to achieve this voluntary emission target effectively, public and private sector have tried to introduce appropriate mitigation methods in various sectors such as energy, transportation and so on. In particular, the forest sector can play a significant role in mitigating greenhouse gases through carbon sequestration because about 64% of total land is covered by forest in South Korea. In this background, Korea Forest Service has been preparing a policy instrument to make forest carbon credits be traded in the voluntary carbon market which will be launched in 2015 in South Korea. Before introduction of the carbon market, it is needed to analyze companies` demands and preferences to forest carbon credit in advance. This research aimed to estimate the willingness to pay (WTP) of private companies in South Korea for carbon credits from forest offset projects such as domestic forest management activities and REDD (reducing emissions from deforestation and forest degradation) in developing countries. We postulate null hypotheses that there is no difference in the WTP according to types of forest offset projects. The face to face interview was conducted with 200 companies engaged in various types of business in 2011. 70 valid responses were analyzed to elicit the value of WTP for carbon credits per ton of CO2 equivalent by non-parametric approach (Turnbull estimation). We found that there was a difference in WTP according to the area of forest offset project. Estimated WTPs for forest carbon credits were 4.55~7.86 USD/tCO2 in domestic project and 6.64~11.05 USD/tCO2 in foreign project. These results can contribute to designing forest carbon market considering primary consumers.
High-frequency Plant Regeneration from Cultured Flower Bud Receptacles of Allium hookeri L.
Koo, Ja Choon Korean Society of Horticultural Science 2014 원예과학기술지 Vol.32 No.5
Allium hookeri L. (Alliaceae family) is an important ethnomedicinal plant native to the Himalayan region of Asia. The aim of this research was to establish a high-frequency plant regeneration system for in vitro propagation of A. hookeri. Among the tissue types examined, receptacle explants derived from immature flower buds showed the highest regeneration rate of shoots ($93.33{\pm}4.63%$), roots ($76.67{\pm}7.85%$), and calli ($80.00{\pm}7.43%$) when cultured on Gamborg B5 (B5) medium containing $10{\mu}M$ 6-benzylaminopurine (BA) + $1{\mu}M$ naphthalene acetic acid (NAA), $0.5{\mu}M$ BA + $5{\mu}M$ NAA, and $1{\mu}M$ BA + $10{\mu}M$ NAA, respectively. Shoot multiplication was superior when cultured in liquid rather than on solid medium and relatively high concentrations of BA, ranging from 5 to $10{\mu}M$. Efficient bulblet formation following root induction from shoot clumps was achieved with culture in liquid B5 medium containing 7% (w/v) sucrose. Regenerated bulblets were successfully acclimatized to ex vitro conditions with a greater than 95% survival rate. By this method, a maximum of 62 plantlets per receptacle could be propagated within 9 weeks of initial culture. The in vitro propagation system established in this study will promote A. hookeri biotechnology, including large-scale production of healthy and aseptic clones, preserving parental genotypes with desirable traits, and genetic manipulation to enhance medicinal value.