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Improved Micropropagation of Root Chicory, Cichorium intybus L. var. sativus.
Lim,Jung-Dae,Yang,Deok-Chun,Lee,Hyeon-Yong,Kim,Jong-Dai,Lee,Jin-Ha,Sung,Eun-Soo,Yu Chang-Yeon 한국자원식물학회 2004 Plant Resources Vol.7 No.1
The establishment of an efficient protocol for plant regeneration and micropropagation from leaf explant cultures of Chicory, Cichorium intybus L. var. sativus. is reported. Callus formation rate appeared 100% from explant in all growth regulators, but calli formed in the prensence of naphthaleneacetic acid (NAA) were appeared very compact and non-embryogenic state. The regenerated shoots were obtained from leaf explant cultures on solid MS medium containing different concentrations of cytokinins and auxin. The highest number of shoots (5.7) per explant and shoot growth (2.8cm) was obtained on MS medium containing 0.1 mg BAP L^-1 and 0.1 mg NAA L^-1. Indole acetic acid was the most suitable auxin for root formation among three auxins tested. 2,4-D had no effect on shoot and root formation.
혼수상태의 외상환자에서 초음파를 이용한 흉부 및 복부손상의 진단
임경수,이강현,이진웅,이부수,황성오,유수영,강성준 대한응급의학회 1995 대한응급의학회지 Vol.6 No.1
The need for rapid diagnosis and treatment of life-threatening thoracic and intra-abdominal injury result in controversy over the appropriate triage of unconscious blunt trauma patients. To aid in early decisions for these patients, a prospective analysis of 98 patients with glasgow coma scale(GCS) scores≤8 was undertaken. Although intra-thoracic and intra-abdominal injuries were frequently identified based on systolic blood ressure, the use of clinical signs alone resulted in more missed injuries than did using the emergency ultrasonography. In normotensive patients(n=34), intra-thoracic injuries was identified in one patient(2.9%), and intra-abdominal injuries were in 7 patients(20.6%). In shock(systolic blood pressure<90mmHg) Pa-tients(n=64), thoracic injuries and intra-abdominal injuries were diagnosed in 8(12.5%) and 27 patients(42.3%), thoracic and abdominal combined injuries were identified in 11 patients(17.2%). The sensitivity, specificity, and accuracy of emergency ultrasonography were 96.3%, 91.7%, 94.9%. This study suggests that all unconscious trauma patients undergo immediate emergency ultrasonography to prevent missing life-threatening injuries.
林銖遠,朴根守,張龍洙 慶北大學校 師範大學 體育學硏究會 1992 體育學會誌 Vol.20 No.-
The primary purpose of this study was to elucidate structure of consciouness about An-Dong Citizen's life be in sports and grope a device of activation. In order to meet this purpose, 977 questionnaires was analyzed according to sex, age, education, occupation and income. The results were as follows; 1) 84.4% of whole subjects felt necessity of Life be in Sports in every day life. in view of sex, 87.3% of males and 81.5% of females felt the necessity. 2) 81.8% of subjects regarded life be in sports as means of health improvement. 3) About the local facilities of life be in sports, only 9.4% of subjects satisfied and 64.9% of subjects revealed dissatisfaction. 4) Preference order in relation to the expansion of facilities appeared as follews; swimming pool(26.9%), tennis court(18.6%), gymnasium(11.4%). badminton court(11.5%), aerobic hall(5.8%) and bowling range(4.2%). 5) About making sport club, 82.4% of Whole subjects revealed affirmative answer. 6) About present participation in life be in sports, 30.1% of subjects answered being participated, but 69.9% subjects answered not being participated. 7) About necessity of coach of Life be in soprts, 66.0% of subjects felt the necessity. 8) The main reason of not attendance in Life be in sports were lack of leisure, economical difficulty, insufficiency of facilities. 9) 13.0% of subjects wanted to participate in tennis, 11.7% to swimming, 11.3% to badminton, 8.6% to mountaineering, 7.6% to aerobic, and 6.9% to bowling if circumstance permit. and then, the major device for activation of life be in sports were utilization and expansion of facilities, development of program, arrangement and nurture of coach, public information about life be in sports, support and construction of sports club, financial and administrative support.
당뇨병 환자에서 혈장 Thrombin-Antithrombin Ⅲ 및 Plasmin-α_2-Plasmin Inhibitor 복합체의 임상적 의의
김경욱,김은숙,정상수,윤수지,박우일,이준희,남수연,안철우,문병수,김경래,차봉수,송영득,임승길,이현철,허갑범 대한당뇨병학회 2002 Diabetes and Metabolism Journal Vol.25 No.5
연구배경:당뇨병 환자에서 혈액응고 및 섬유소용해 체계의 이상경향이 있어 그 결과로 여러 혈관합병증의 발생위험이 높다는 사실은 널리 알려져 있다. 그 기전은 아직 확실히 밝혀지지 않았으나, 고혈당으로 인한 혈장 단백질들의 비효소성 당화작용이나 산화성 스트레스로 인한 유리 라티칼 작용으로 응고항진이나 섬유소용해 활성의 저하를 유발하는 것으로 생각되고 있다. 최근 응고 및 용해인자와 그 억제자의 복합체들의 증가가 이 상태를 비교적 예민하게 반영한다고 알려져 있다. 방법:본 연구에서는 당뇨병 환자 101명과 정상 대조군 20명에서 혈장내 thrombinantithrombin complex(TAT)와 plasminα₂plasmin inhibitor complex(PIC)를 측정하여 비교하고, 당뇨병 환자에서 미세혈관 합병증과 대혈관합병증의 유무에 따른 차이와, 이미 혈관 질환의 위험인자로 알려져 있는 인자들간의 상관성을 알아보고자 하였다. 결과:1. 환자의 분포를 살펴보면 혈관합병증이 있는 군은 85명, 혈관합병증이 없는 군은 16명이었고, 평균연령은 각각 57.9±14.1세, 49.9±16.6세로 혈관 합병증이 있는 군에서 더 나이가 많았고, 체질량지수는 23.2±3.4㎏/㎡, 24.1±3.4㎏/㎡로 두 군간 유의한 차이는 없었다. 또 두 군간의 혈압 및 HbA1c, 공복혈당 및 인슐린과 Cpeptide, 총 콜레스테롤, 중성지방, HDL콜레스테롤, Lp⒜는 유의한 차이가 없었고, 미세혈관합병증이 있는 군에서 당뇨병의 유병기간이 길었다. 2. TAT 및 PIC의 농도는 정상 대조군에서는 2.8±1.2 ng/mL, 240.4±69.7 ng/mL이었고, 당뇨병 환자군에서는 9.5±22.6 ng/mL, 472.2±258.7 ng/mL이었다. TAT와 PIC 모두 당뇨병 환자군에서 정상 대조군에 비해 유의하게 증가되어 있었고(p<0.001), TAT/PIC ratio는 두 군간 차이가 없었다. 3. 당뇨병 환자의 혈관합병증에 따른 TAT 및 PIC, fibrinogen 농도는 합병증이 없는 군은 각각 4.1±2.4ng/mL, 362.2±272.0ng/mL, 322.7±102.4mg/mL으로 PIC와 fibrinogen의 증가를 보였으나, 연령을 보정한 후에는 통계학적 유의성은 없었다. 또 대혈관 합병증군에서는 각각 6.0±4.9 ng/mL, 507.4±321.6 ng/mL, 427.1±194.7 mg/dL이었으며 미세·대혈관 합병증군에서는 10.4±6.4 ng/mL, 484.8±269.7 ng/mL, 388.4±132.4 mg/dL으로 TAT의 증가를 보였으나 역시 연령을 보정한 후에는 통계학적 유의성은 없었다. 4. 미세혈관합병증군에서 HbA1c(>8%)가 높은 군의 PIC 농도가 유의하게 높았고(p=0.049), 대혈관합병증군에서 HbA1c(>8%)가 높은 군의 총 콜레스테롤 농도가 유의하게 높았다(p=0.042). 5. 총 당뇨병 환자군에서 PIC는 fibrinogen과 HbA1c와 양의 상관관계를, BMI와 음의 상관관계를 보였으며(r=0.47, 0.31,-0.25), 혈관 합병증이 없는 당뇨병 환자군에서만 TAT는 HbA1c와 양의 상관관계를 보였다(r=0.67). 결론:이상의 결과에서 혈장 TAT 및 PIC 농도는 당뇨병 환자에서 정상 대조군에 비해 의미있게 증가되어 있었고, 당뇨병 환자군에서는 연령의 증가와 유병기간이 혈액응고항진 및 용해의 장애에 큰 역할을 함을 알 수 있었으며, 총 당뇨병 환자군에 PIC와 HbA1c와 양의 상관관계를, BMI와 음의 상관관계를 보였으며 혈관 합병증이 없는 당뇨병 환자군에서만 TAT는 HbA1c와 양의 상관관계를 보였다. 따라서 당뇨병 환자에서 혈액응고 및 용해의 장애가 동반되어 있다고 볼 수 있으며, 혈장 TAT 및 PIC는 혈관합병증으로의 진행을 예측하는 지표로서 유용하리라 생각된다. 또 혈당조절정도와 상관성이 있으므로 혈당조절후에 추적검사를 시행하여 합병증의 예방이 가능한지 추후 연구가 필요하리라 생각된다. Background : Abnormality of coagulation and fibrinolystic system is known as a predisposing factor of vascular complication in diabetes. Although the pathogenesis is not well known, non-enzymatic glycation reaction and the increase in production of free radicals due to an increased oxidative stress may be linked to the hypercoagulibility and hypofibrinolytic activity. As indices of abnormality in coagulation and firinolysis in peripheral blood, plasma thrombin-antithrombin Ⅲ complex (TAT) and plasmin-α_2-plasmin inhibitor complex (PIC) were measured. The purpose of this study was to clarify whether hypercoagulability exists in diabetic patients with or without vascular complication. Methods : In our study, we measured plasma thrombin-antithrombin Ⅲ compelx (TAT) and plasmin-α_2-plasmin inhibit or complex (PIC) in 101 diabetic subjects and 20 controls. Comparing TAT and PIC levels in diabetic microvascular complication group, diabetic macrovascular complication group and controls, we examined correlation between risk factors associated with diabetic vascular complication. Results : 1. The group with diabetic vascular complication was older than group without complication. There was no significant difference in BMI, blood pressure, HbA_ic, blood sugar level, insulin, C-peptide, serum creatinine, total cholesterol, triglyceride, HDL-cholesterol, Lp (a) between two groups. The group with diabetic microvascular complication had longer duration of diabetes. 2. Concentration of TAT and PIC were 2.8±1.2 ng/ mL, 240.4±69.7 ng/ mL in controls and 9.5±22.6 ng/ mL, 472.2±258.7 ng/ mL in diabetic patients, respectively. TAT and PIC were significantly higher in diabetic patients than in control (p<0.001). But TAT/PIC ratio was no significant difference between two groups. 3. In diabetic patients, concentration of TAT and PIC and fibrinogen were respectively 4.1±2.4 ng/ mL, 362.2±272.0 ng/ mL, 322.7±102.4 mg/ dL in group without vascular complication and 5.3±4.1 ng/ mL, 529.5±258.7 ng/ mL, 374.9±106.2 mg/ dL in group with microvascular complication, which group had increase in PIC and Fibrinogen but no significance after correction of age. Concentration of TAT and PIC and Fibrinogen were 60.±4.9 ng/ mL, 507.4±321.6 ng/ mL, 427.1±194.7 mg/ dL in macrovascular complication, and 10.4±6.7 mg/ mL, 484.8±269.7 ng/ mL, 388.4±132.4 mg/ dL in combined vascular complication which group showed increase of TAT but also had no significant increase after correction of age. 4. In diabetic microvascular complication patients, group of high HbA_1c (>8%) (p=0.049) had significant high PIC concentration. In diabetic macrovascular complication patients, group of high HbA_1c (>8%) (p=0.042) had significant high total cholesterol concentration. 5. In all diabetic patients, PIC was positively correlated with fibrinogen and HbA_1c and negatively correlated BMI (r=0.47, 0.31, -0.25). Only in daibetic patients without angiopathy, TAT was positively correlated with HbA_1c (r=0.67). Conclusion : In this study, plasma TAT and PIC concentration significantly increased in diabetic patients compared with controls, and PIC was increased in group with microvascular complication, TAT were increased in group with combined micro macrovascular complication. However, there was no significance relationship existed when correctinf for age. PIC was correlated with HbA_1c. TAT was correlated with HbA_1c only in the group without angiopathy. Abnormality of coagulation and fibrinolysis were combined in diabetes, plasma TAT and PIC can be used as an index of vascular complication. Also we found the correlation with the degree of the blood glucose control. Therefore we need follow up study for the possibility of prevention of vascular complication after controlling the blood glucose to age-matched patients (J Kor Diaabetes Asso 25:354~363, 2001).
ABUTMENT의 경사가 CORE의 응력-변형률에 미치는 영향
임희대,박수용,우원재 忠南大學校 産業技術硏究所 1995 산업기술연구논문집 Vol.10 No.1
Cracking of core presents a serious hazard resulting in possible leakage and failure. For a realistic evaluation of cracking of core one must take into account irregular steep abutment. The present paper is concerned with the most dangerous transverse movements in the embankment which result from non-uniform settlement along the abutments. In this study, the effect of steepness of abutment has been analyzed for 100m high central trapezodial cores with abutment slopes of 1:0.25, 1:0.5, 1:0.75, and 1:1 respectively. Numerical analysis of longitndinal sections of the embankment has been carried out by the application of finite element method. The nonlinear and stress-dependent stress-strain properties of soil is approximated by using a hyperbolic model. The results of the analyses indicate that there is a decrease of vertical stress due to arching action. This decrease is more for steeper slope than for flatter slope.
임경택,정수영 충남대학교 산업기술연구소 2000 산업기술연구논문집 Vol.15 No.1
Experimental investigation was conducted to study the shear capacity of recycled aggregate concrete beams without shear reinforcement. The primer objective of the study was t investigate the suitability of using recycled aggregate concrete beams. In designing the specimens, shear-span/depth ratio and aggregate consideration(washed or non-washed aggregate) were chosen as main parameters. As a result, this study, to know the shear capacity of recycled aggregate concrete beams with the main variables such as shear span ratio and weather the recycled aggregate was washed or not, following conclusions come out. 1.In the 3-point flexural strength examine certain comparison of flexural strength between natural and recycled aggregate concrete wasn't come out, because it is failure specimen with early flexural crack simultaneously. 2.Shear strength of recycled-aggregate concrete beam under shear span ratio a/d=3 was higher than ACI(318-95) Code, but lower over a/d =4. 3.Ultimate shear strength of recycled aggregate concrete beam was higher than ACI(318-95) Code and it was very high on shear span ratio a/d =2.
임수덕 大韓法醫學會 1981 대한법의학회지 Vol.5 No.1
A drug is defined as any substance used in diagnosis, treatment, or prevention of disease, and an adverse drug reaction may be considered as any unintended or undesired consequence of drug use. With the rapid development of diagnostic and therapeutic drugs, adverse reactions to these agents have become an increasingly important medico-legal problem. Although drug reactions may involve any organ system, cutaneous eruptions are more often recognized because of their visibility. So cutaneous drug eruptions play an important rloe in early detection of the adverse drug reactions and management for it. In the adverse drug reactions there are systemic and cutaneous manifestations. The most common systemic reactions in order of frequency are nausea, drowsiness, diarrhea, vomiting, rash, arrhthymias, itching, hyperkalemia, and fever. Severe systemic reactins may lead to be fatal, but in the mild cases the reaction is only limited on the skin. The case/fatality ratio from drug-induced reactions in hospitalized patients varies from 0.24 to 2.9%. Cutaneous reactions occur in 2 to 3 percent of medical inpatients. The morphologic aspects of drug eruption are protean and frequently bizarre. It includes every possible type of skin lesion and many reaction patterns, specific and nonspecific. So it must be kept in mind that the diagnosis of drug eruptions rests upon constant awareness of the capacity of drugs, like any other chemicals, to produce disease.
무수프탈산을 취급하는 근로자들의 알레르기 질환 유병 실태
임현술,김수근,김기식 大韓産業醫學會 1998 대한직업환경의학회지 Vol.10 No.4
Phthalic anhydride(PA) is widely used in the production of alkyd and unsaturated polyester resins. It has been reported that some workers exposed to PA have developed dermatitis, rhinitis and asthma. The authors intended to investigate the prevalence of PA induced allergic diseases and to develop preventive measures of occupational diseases. Forty-five male workers from PA production and handling were selected as an exposure group. Forty-four male workers not handling PA at tie same factory were chosen as a reference group. A symptom questionnaire, doctor's examination, eosinophil count, serum-total IgE and PA-specific IgE were done on the subjects. There were no significant differences in age, educational level. tenure or smoking habits between groups. Most clinical symptoms were significantly more frequent among the exposure group than the reference group: sneezing(62.2%), rhinorrhea(57.8%), nasal stuffiness(53.3%), coughing(44.4%) and nasal itching(35.6%) were the major symptoms among the exposure group(p<0.01, p<0.05). There were no significant differences in eosinophil count and serum-total IgE between groups but the mean level in PA-specific IgE, in the exposure group, was statistically significantly higher than the reference group(p<0.01). Abnormal rates of eosinophil count, serum-total IgE and PA-specific IgE were not significantly different from each other. The prevalence rate was 68.9%(31 cases) for allergic rhinitis in the exposure group, statistically significantly higher than the reference group(p<0.01). The prevalence rate was 6.7%(3 cases) for bronchial asthma and contact dermatitis in the exposure group, not more statistically significant than the reference group. In the exposure group, the prevalence rate for age, educational level, tenure, smoking habits and use of protective devices are not statistically significant. In PA specific IgE, sensitivity is 10.8%, specificity is 100.0%, positive predictability is 100.0% and negative predictability is 61.2% when criteria point is 3.5 KU/ιor above. As a result, it was recognized that the exposure group had a high prevalence of PA induced allergic rhinitis. Also, in diagnosis of PA induced allergic diseases, medical histories and specific symptoms were the most important where as eosinophil count, serum-total IgE are the only indirect informations. PA-specific IgE has been needed to evaluate the meanings more.
임희대,박수용 忠南大學校 産業技術硏究所 1995 산업기술연구논문집 Vol.10 No.1
The behavior of an unsaturated soil tested under undrained loading conditions depends on the magnitude of the pore-air and pore-water pressure developed. This paper derives pore pressure parameters that can be utilized in determining the pore pressure response of the compacted clayey materials. Pore pressure parameter expressions are also derived for the pore-water pressures induced as a result of isotropic stress changes and deviator stress changes during undrained loading. The parameters required to computation in the proposed model can be readily determined from the conventional triaxial compression tests. To examine the degree to which the proposed pore pressure model reflects the results of triaxial tests, The back analyses carried out, It is shown from the examination that agreements are generally satisfactory.