난소의 소세포암종, 고칼슘혈증형 대세포 변종 : 1예 보고 A Case Report

이종실,이종학,김유경,박선후,고경혁 대한병리학회 2001 Journal of Pathology and Translational Medicine Vol.35 No.4

Guar gum의 섭취 수준과 기간이 흰쥐의 영양소 흡수율에 미치는 영향

김상연,선영실,장유경 漢陽大學校 韓國生活科學硏究所 1993 韓國 生活 科學 硏究 Vol.- No.11

This study was performed to investigate apparent absorption of nutrients according to level and duration of guar gum intake. Eighteen male rats of Sparague-Dawley strain weighing 50g were blocked into three groups : FF, 5G, 10G(Fiber Free, 5% Guar gam, 10% Guar gum). Balance studies were conducted at 2nd week, 4th week and 8th week. Feed in-take body weight gains, apparent absorption of protein, fat, Ca and P were measured. The results obtained in this study were summarized as follows : (1) Feed intake was not significantly different among the groups during each balance studies. Weiht gain was decreased according to level of guar gum intake during each balance studies. (2) Protein apparent absorption of 5G were not significantly different with that of 10G at 2nd week and 4th week but at 8th week protein apparent absorption of 10G was singificantly lower than that of 5G According to duration of guar gum intake, protein apparent absorption of FF and 5G were not significantly different. Wherease protein apparent absorption of 10G was significantly decreased at 8th week. (3) Fat and Ca apparent absorption were not significantly different among groups at 2nd week. Fat apparent absorption of 10G was not significantly lower than that of 5G at 4th week and 8th week. According to duration of guar gum intake, fat and Ca apparent absorption of FF and 5G were not significant different. But fat and Ca apparent absorption of 10G was significantly decreased according to duration of fur gum intake. (4) Phosphorus apparent absorption were not significantly different among groups at 2, 4th week. But phosphorus apparent absorption of 10G were significantly higher than that of 5G at 8th week. According to duration of guar gum intake, phosphorus apparent absorption of FF and 10G were not influenced. But Phosphorus apparent absorption of 5G was significantly decreased according to duration of guar gum intake. From the results of this study, we could recommend 5% guar gum in diet of obese man and di abetics.

신규간호사와 프리셉터가 인지하는 프리셉터의 교수효율성 비교

김지양,김영선,김춘실,박현숙,신미영,윤연숙,조유숙,박미미,유문숙 한국간호과학회 간호행정학회 2009 간호행정학회지 Vol.15 No.2

Objective: The purpose of this study was to compare preceptors and new graduate nurses on their perception of preceptor teaching effectiveness. Methods: The participants were 90 new nurses and 90 preceptors who worked in A medical center. The data were collected from July 1 to September 30, 2008. A cross-sectional descriptive survey was done using a structured questionnaire. Results: New nurses' perception (4.07±.44) of the preceptor teaching effectiveness was significantly higher than perception of the preceptors (3.57±.37). Fifty five percent of new graduate nurses reported a lack of coherence in the preceptor's practice guidelines. For 5 causal factors classified on the teaching effectiveness, the factor of 'Professional knowledge and ability' showed the highest score, but 'Interpersonal and communication skill' got lowest score for both group. Preceptors responded that they did not have enough time to teach well because of their heavy workloads. Conclusions: These results suggest that the preceptors need appropriate compensation and education opportunities, and new graduate nurses need consistent education by the teaching professionals. Therefore, it is important to give preceptors full charge of the preceptorship. Also, it will be necessary to develop education programs to enhance interpersonal and communication skill for preceptors and new nurses.

The Anti-tumor Activity of Vitamin C via the Increase of Fas (CD95) and MHC I expression on Human Stomach Cancer Cell Line, SNU1

Yu, Yeon-Sil,Bae, Se-Yeon,Kim, Hye-Min,Kim, Ye-Jin,Chu, Nag-Bum,Chu, Nag-Kyun,Kang, Jae-Seung,Lee, Wang-Jae The Korean Association of Immunobiologists 2011 Immune Network Vol.11 No.4

It is already known that high concentration of vitamin C induces apoptosis on tumor cells. However, there is no report regarding the function of vitamin C on the modulation of immune susceptibility of cancer. Therefore, we investigated whether vitamin C can modulate immune susceptibility of tumor cells, especially on the induction of Fas-mediated apoptosis. First, the optimal concentration of vitamin C, which cannot induce damages on tumor cells for 36 hrs. We found that 2 mM of vitamin C did not show harmful effect. In addition, the optimal concentration of agonistic anti-Fas Abs for 18 hrs was examined. As a result, 400 ng/ml of agonistic anti-Fas Abs did not induce apoptosis on tumor cells. Next, we tried to find the effect of 2 mM of vitamin C on the modulation of the susceptibility to agonistic anti-Fas Abs. When tumor cells were cultured with 400 ng/ml of agonistic anti-Fas Abs for 18 hrs, after pre-treatment with 2 mM of vitamin C for 24 hrs, viability of cells was decreased. Interestingly, we found that the expression of Fas (CD95) and MHC class I was increased by the treatment of vitamin C. Taken together, vitamin C increases the susceptibility of tumor cells to anti-Fas Abs and the expression of Fas (CD95) and MHC class I on tumor cells.

Expression of antimicrobial peptide PAJE in Escherichia coli

Yu-Sil Hong,Sung Wan Kim,Kwang Ho Choi,Tae Won Goo,Seong Ryul Kim 한국응용곤충학회 2013 한국응용곤충학회 학술대회논문집 Vol.2013 No.10

The antibiotic peptide PAJE (RWKIFKKPFKISIHL-NH2), designed incorporating the N-terminal α-helical segments of papiliocin and jelleine, is a 15-residue hybrid peptide that has a broad spectrum of activity against Gram-negative, positive bacteria and fungi. In this study, we successfully expressed bioactive PAJE in Escherichia coli cells that are highly sensitive to this peptide. For the efficient production of peptide, we synthesized gene encoding PAJE, and fused the sequence in-frame to ketosteroid isomerase (KSI) gene to construct an expression vector pET29b-PAJE-KSI, which was then used to transform E. coli BL21 (DE3). The fusion protein PAJE-KSI was expressed as inclusion body at high level (more than 30% of the total proteins). Recombinant PAJE was easily released by cleavage of the fusion protein with cyanogen bromide (CNBr). Subsequently, we purified the recombinant PAJE by FPLC chromatography. The purified PAJE displayed considerably antibacterial activity identical to that previously reported for chemically synthesized PAJE. The results indicated that successful expression of PAJE in E. coli cells and efficient procedure for purification may lead to a cost-effective platform for the mass production of PAJE.

Polyarteritis Nodosa of the Gallbladder : A Report of Two Cases 2례 보고

Yu, Eun Sil,Kim, Yoong Il Seoul National University 1986 The seoul journal of medicine Vol.27 No.4

현대 중국의 의료 제도 엿보기 : 영화 『나는 약신이 아니다(我不是藥神)』를 중심으로

유연실(Yu, Yon-Sil) 의료역사연구회 2021 의료사회사연구 Vol.8 No.1

2018년 7월 18일 중국에서 개봉한 나는 약신이 아니다(我不是藥神)는 만성골수성백혈병(Chronic Myelogenous Leukemia, CML) 치료제인 글리벡(Glivec)을 매개로 벌어지는 백혈병 환자들의 애환을 다룬 영화이다. 이 영화는 백혈병 환자였던 ‘루용(陸勇)’이 글리벡의 인도산 복제약을 중국에 불법으로 들여와 환자들에게 공급하다 2013년 8월 체포되었던 실제 사건을 바탕으로 제작되었다. 원무예(文牧野) 감독은 ‘루용’ 사건을 토대로 영화 속에서 다국적 제약회사의 항암치료제 독점 생산, 인도산 복제약의 유통, 질병과 빈곤의 악순환 등 다양한 중국의 의료 문제를 폭로하였다. 무엇보다 이 영화는 글로벌 제약회사의 환자 생명권을 담보로 한 지적재산권 행사 문제 및 중국 의료보험제도의 모순을 고발하고 있다는 점에서 시사하는 바가 크다. 본고는 이 영화를 통해 의료와 관련된 두 가지 측면을 중점적으로 검토하였다. 첫째, 다국적 제약회사의 의료 시장 독점과 환자의 ‘생명권’ 보장이라는 문제를 검토하였다. 스위스의 다국적 제약회사인 노바티스사(Novartis)가 개발한 글리벡은 인류 최초의 표적 항암제로서 백혈병 환자들에게 생명의 희망을 안겨 주었지만, 천문학적인 약값으로 인해 환자들에게 ‘절망의 약’으로 불리게 되었다. 이와 같은 다국적 제약회사의 횡포에 대항하여, 중국에서는 루용으로 대표되는 백혈병 환우 집단이 자신들의 생명권을 수호하기 위해 인도산 복제약을 수입하여 비밀리에 유통시키는 불법적인 행동을 전개하였다. 이 사건을 계기로 중국 정부는 21세기 생명정치의 변동 속에서 환자의 생명권과 제약회사의 경제적 이익을 어떻게 양립시킬 것인가라는 문제에 직면하게 되었다. 둘째, 권력의 병리학과 관련된 의료의 불평등 문제이다. 중국 의료보험제도의 계층적⋅지역적 불평등과 수입 항암제에 대한 높은 관세로 인해 환자들은 질병에 걸리면 높은 약값과 치료비를 충당하기 위해 경제적 빈곤에 허덕이고, 또한 경제적으로 어려운 사람들이 의료적으로 소외되어 더욱 쉽게 질병에 걸리는 악순환이 반복되었다. 루용 사건을 계기로 중국 정부는 의료보험의 보장성 확대, 수입 항암제에 대한 규제 완화, 복제약의 생산 증대 등 다양한 측면에서 의료제도의 개선을 위해 노력하였다. 그러나 중국 정부는 의약품에 대한 연구 개발 투자 확대, 제약회사의 이익 규제, 환자집단의 자율성 보장 등 측면에서 여전히 많은 문제를 해결해야 한다. 역설적으로 사회주의 체제하에서 자본에 의해 독점되는 의료 시장과 질서가 ‘약신’을 필연적으로 만들어 냈다는 점을 고려한다면, 국가 권력이 의료의 불평등 문제 해결과 의약품의 공공성 확대를 위해 어떠한 노력을 전개해야 할 것인가라는 해답을 찾을 수 있을 것이다. “Dying to survive(我不是藥神)” released in China on July 18, 2018. This movie tells the sorrow and pain of chronic myeloid leukemia(CML) patients who use an expensive anticancer drug called Glivec. The film is made on the basis of actual events, that is, the real protagonist “Lu Yong(陆勇)” of the film is unable to pay for the expensive medicine because he suffers from leukemia. Since 2004, he has imported generic drugs produced in India and illegally circulated them to patients. He supplied India-made generic drugs to patients at the original price, but was arrested by the police in August 2013 for the crime of selling counterfeit drugs. Based on the “Lu Yong” incident, director Wen Muye(文牧野) exposed various Chinese medical problems in the film such as the exclusive production and sales of anti-cancer therapeutics by multinational pharmaceutical companies, the circulation of illegal generic drugs, the vicious circle of disease and poverty. Most importantly, this film exposes the conflicts between the pursuit of intellectual property rights secured by multinational pharmaceutical companies and the Chinese medical insurance system. This article focuses on two issues through this film. First, this article analyzes the monopoly of the medical market by multinational pharmaceutical companies and the protection of patients “right to life”. Glivec, developed by Novartis of Switzerland, is the first new anti-cancer drug that targets the tumorigenesis mechanism in humans, it brings hope of life to leukemia patients. However, due to the excessively expensive drug fees, it is called by the patients “The Medicine of Despair”. Facing the rampant dominance of multinational pharmaceutical companies, China s leukemia patient group represented by “Lu Yong” imported Indian-made generic drugs for secret circulation in order to protect their right to life. After Lu Yong was arrested by the Procuratorate, the leukemia patient requested the Chinese government to release Lu Yong and exposed the difficult situation of the leukemia patient to the media. In this 21st century biopolitical change, the Chinese government is faced with the problem of how to balance the patient’s right to life with the economic interests of pharmaceutical companies. Second, this article explores the issue of medical inequality. Due to the inequality of China’s medical insurance system and the high tariffs on imported anti-cancer agents, leukemia patients become poor in order to pay high drug and treatment fees after suffering from the disease, and it is difficult for people with financial difficulties to enjoy medical services. So it is more likely to suffer from the vicious circle of disease recurring. After the “Lu Yong” case, the Chinese government has improved the medical security system in a number of ways, including expanding the coverage of medical insurance, relaxing restrictions on imported anticancer agents, and increasing the production of cloned drugs. However, the Chinese government still needs to solve many problems in terms of investment in the research and development of pharmaceuticals, control of the interests of pharmaceutical companies, and protection of activeness and behaviorality of the patient. Paradoxically, if one considers that the medical market and order monopolized by capital under the socialist system will inevitably create a “medicine god(藥神)”, the state power should do its utmost to solve the problem of medical inequality and expand the publicness of pharmaceutical products.

노동과 출산의 이중변주

유연실(YU YON SIL) 중국근현대사학회 2013 중국근현대사연구 Vol.60 No.-

The People’s Republic of China, under the slogan “Women hold up Half the Sky”, called out a wide range of women to the public sphere of society, politics, economics. In this, women are recognized identity as a member of society through labor, and also obtained confidence that can borrow the authority of the state, to challenge gender order. The Chinese government is trying to ensure the labor of women and increase the production volume, such as through the dissemination of new midwifery and the implementation of painless childbirth. It was also resolved to some extent the burden of child-rearing of women in the installation of the nursery. But, in fact, the female body, is plagued by double whammy of reproductive labor and wage labor, and had to function of the body in order to produce the labor(population) and goods under the name of economic development of the country. Since 1955, birth control education and publicity at the national dimension is performed actively, medical contraception has spread, hard of pregnancy and childbirth of women was reduced to some extent. In other words, the woman was released to some extent from the re-production labor, but contraceptives supply is not facilitate and the effect of contraceptive markedly dropped. In this situation, women had to practice on their own bodies abortion and sterilization. After all, the spread of medical technology of childbirth, women were released to some extent from the re-production labor, but women were difficult to obtain real physiological free. Because Countries is constantly monitoring control women’s bodies through medical. Since 1978, ‘one child policy’ is currently being carried out and to keep the policy in the context of China, it is intended to mean that the rights of women’s physical autonomy and growth are controlled by their country yet.

PARTIAL SEQUENCING OF ROOT CDNA CLONES OF Brassica napus TO GENERATE EXPRESSED SEQUENCE TAGS AND TO PROVIDE A PLANT GENE RESOURCE

Park, Yu-Shin,Kwak, June-Myoung,Kwon, O-Yu,Kim, Yong-Sung,Lee, Dae-Sil,Cho, Moo-Je,Lee, Hyung-Hoan,Nam, Hong-Gil Gyeongsang National University Chinju, Korea 1992 Plant molecular biology and biotechnology research Vol.1992 No.

만성 폐쇄성 폐질환 평가 테스트의 유용성

김유은 ( Yu Eun Kim ),이상수 ( Sang Su Lee ),김차영 ( Cha Young Kim ),이승훈 ( Seung Hun Lee ),임수진 ( Su Jin Lim ),조유지 ( Yu Ji Cho ),정이영 ( Yi Yeong Jeong ),김호철 ( Ho Cheol Kim ),황영실 ( Young Sil Hwang ),이종덕 ( Jong D 대한결핵 및 호흡기학회 2011 Tuberculosis and Respiratory Diseases Vol.71 No.4

Background: A chronic obstructive pulmonary disease (COPD) assessment test (CAT) has recently been developed as a short and simple method for assessing the quality of life in COPD patients. The object of this study was to assess the usefulness of the Korean version of the CAT for assessing COPD patients in an outpatient clinic. Methods: The study included 60 COPD patients in a stable state from an outpatient clinic. The authors investigated the frequency of acute exacerbation during aprevious year through reviewing medical records. We evaluated the spirometry test, a 6-min walk distance test, and obtained the MMRC dyspnea scale, the Korean version of the CAT, and the BODE index at the time of visit. To assess the usefulness of the CAT, correlations between the CAT and other methods were evaluated. Results: The mean age of patients was 68.3±8.6 years and 95% of patients were male. There was a significant correlation between the CAT score and FEV1% (r=?0.323, p=0.012), the frequency of acute exacerbation (r=0.292, p=0.024), the MMRC dyspnea scale (r=0.554, p<0.001), the BODE index (r=0.380, p=0.003), and 6 MWD (r= ?0.372, p=0.004). The mean CAT score increased according to the GOLD stage (stage 1, 10.7±4.5; stage 2, 13.1±7.9; stage 3, 16.3±6.2; stage 4, 16.5±14.8; p=0.746). Conclusion: The CAT was shown to be useful for the assessment of COPD severity. Therefore, the CAT is an easily applied and simple method for assessing COPD severity in an outpatient clinic.