수확후 채소류에 발생하는 진균독소의 탐색과 방제(Ⅱ) 이병된 양념 채소류(양파, 마늘, 고추)에서 주요 진균독소 검출

정일민,주호종,심성철,백수봉,유승헌 충남대학교 생물공학연구소 1999 생물공학연구지 Vol.7 No.-

수확후 Alternaria, Penicillium 및 Fusarium에 이병된 고추, 양파, 마늘을 HPLC로 주요독소들을 검정한 결과 고추에서 Alternaria 독소의 AOH(alternariol)는 소량∼440㎍/g 및 ALT(altenuene)는 소량∼103㎍/g, TeA(tenuagonic acid)는 249∼342㎍/g 및 AME(alternariol monomethyl ether)는 206∼294㎍/g이 검출되었고 양파, 마늘에서 Penicillium 독소의 citrinin이 2.8∼18.4㎍/g, penicillin-G는 0∼439.0㎍/g, penicillic acid는 0∼10.2㎍/g 및 patulin은 0∼7.0㎍/g 검출되었다. 그리고 양파, 마늘에서 Fusarium 독소로는 fusaric acid가 0∼553.6㎍/g 검출되었을 뿐 deoxynivalenol과 nivalenol은 검출되지 않았다. The major mycotoxins were detected from peppers, onions and garlics infected postharvest pathogens, Alternaria, Penicillium and Fusarium. Analyses of the major mycotoxins were conducted using HPLC. Detected Alternaria mycotoxins per gram of infected postharvest peppers were alternariol (AOH) with amount ranged from small quantity to 440 ㎍/g, altenuene (ALT) with amount ranged from small quantity to 103 ㎍/g, tenuagonic acid (TeA) with amount ranged from 249 to 342 ㎍/g and alternariol monomethyl ether (AME) with amount ranged from 206 to 294 ㎍/g. Penicillium toxins per gram of infected postharvest onions and garlics were citrinin with amount ranged from 2.8 to 18.4 ㎍/g, penicillun-G with amount ranged from no detection to 439.0 ㎍/g, penicillic acid with amount ranged from no detection to small quantity and patulin with amount ranged from no detection to small quantity. Fusarium toxins per gram of infected postharvest onions and garlics were fusaric acid with amount ranged from no detection to 553.6 ㎍/g. However, deoxyrivalenol and nivalenol were not detected from onins and garlics infected by Fusarium.

파종시기가 다른 일미찰의 등숙 중 호화 특성 비교

김미정, 박효진, 이유영, 김선림, 김정태, 우관식, 권영업, 정일민 忠北大學校 農業科學硏究所 2014 農業科學硏究 Vol.30 No.2

This study was conducted to investigate the pasting properties of waxy corn ‘Ilmichal’ during ripening with different sowing date. Starch content of Ilmichal was increased as matured (p<0.05). Analysis of pasting properties with waxy corn starch using a rapid visco analyser (RVA) showed linear changes in peak viscosity, trough viscosity, final viscosity, breakdown and consistency according to ripening. There were significantly positive correlation between starch content of Ilmichal and peak viscosity (0.39*), trough viscosity (0.58***), final viscosity (0.58***), and consistency (0.57**). Differential scanning calorimerty (DSC). The enthalpy of gelatinization was increased as ripening. But onset and peak temperature were decreased. There were significantly positive correlation between starch content of Ilmichal and onset temperature (0.44*), peak temperature (0.38*), and completion temperature (0.59***), on the other hand, crude protein content presented significantly negative correlation between completion temperature (-0.41*).

Factors Influencing the Acrylamide Content of Fried Potato Products

Jin, Yong-Ik,Park, Kyeong-Hun,Chang, Dong-Chil,Cho, Ji-Hong,Cho, Kwang-Su,Im, Ju-Sung,Hong, Su-Young,Kim, Su-Jeong,Nam, Jung-Hwan,Sohn, Hwang-Bae,Yu, Hong-Seob,Chung, Ill-Min The Korean Society of Environmental Agriculture 2016 한국환경농학회지 Vol.35 No.4

BACKGROUND: Acrylamide (CAS No. 79-06-1) is known to be a carcinogenic compound, and is classified as a Group 2A compound by the International Agency for Research on Cancer (IARC, 1994). Acrylamide can be generated during the browning process via the non-enzymatic Maillard reaction of carbohydrates such as reducing sugars and of amino acids such as asparagine, both of which occur at a temperature above $120^{\circ}C$. Potato tubers contain reducing sugars, and thus, this will affect the safety of processed potato products such as potato chips and French fries. In order to reduce the level of acrylamide in potato processed products, it is therefore necessary to understand factors that affect the reducing sugar content of potatoes, such as environmental factors and potato storage conditions, as well as understanding factors affecting acrylamide formation during potato processing itself. METHODS AND RESULTS: Potatoes were cultivated in eight regions of Korea; For each of these different environments, soil physico-chemical characteristics such as pH, electrical conductivity, total nitrogen, available phosphate, and exchangeable cation content were measured and correlations with potato reducing sugar content and potato chip acrylamide levels were examined. The reducing sugar content in potato during storage for three months was determined and acrylamide level in potato chip was analyzed after processing. The storage temperature levels were $4^{\circ}C$, $8^{\circ}C$, or $10^{\circ}C$, respectively. The acrylamide content of chips prepared from potatoes stored at $10^{\circ}C$ or $20^{\circ}C$ for one month was analyzed and the different frying times were 2, 3, 5, and 7 min. CONCLUSION: This study showed that monitoring and controlling the phosphate content within a potato field should be sufficient to avoid producing brown or black potato chips. For potatoes stored at low temperatures, a reconditioning period ($20^{\circ}C$ for 20 days) is required in order to reduce the levels of reducing sugars in the potato and subsequently reduce the acrylamide and improve chip coloration and appearance.

Green synthesis of copper nanoparticles using <i>Eclipta prostrata</i> leaves extract and their antioxidant and cytotoxic activities

Chung, Ill-Min,Abdul Rahuman, Abdul,Marimuthu, Sampath,Kirthi, Arivarasan Vishnu,Anbarasan, Karunanithi,Padmini, Parthasarathy,Rajakumar, Govindasamy D.A. Spandidos 2017 Experimental and therapeutic medicine Vol.14 No.1

<P>The present study outlines the development of a method to synthesize copper nanoparticles (CuNPs) by mixing copper acetate solution with leaf extract of <I>Eclipta prostrata</I> without using any surfactant or external energy. <I>E. prostrata</I> leaf extract function as an excellent reducing agent of copper ions, and the biosynthesized CuNPs are safer for the environment. The powder X-ray diffraction (XRD) pattern provided evidence for the formation of face-centered cubic structure ranging from 23 to 57 nm, with an average size of 31±1.2 nm. Fourier transform infrared spectroscopy (FTIR) was used to identify the biomolecules and capping reagents in the <I>E. prostrata</I> leaf extract that may be responsible for the reduction of copper ions and the stability of the bioreduced nanoparticles. The biosynthesized CuNPs displayed considerable antioxidant capacity. Similarly, <I>in vitro</I> anticancer studies demonstrated the cytotoxicity value of synthesized CuNPs against tested HepG2 cells. The findings of the present study suggested that biosynthesized CuNPs that utilize extracts of <I>E. prostrata</I> may be used for therapeutic application, and thus are a promising nanomaterial.</P>

Cytotoxic Chemical Constituents from the Mushroom of Pholiota adiposa

Ill-Min Chung,Won-Sik Kong,Oh-Kyu Lee,Jeong-Sik Park,Ateeque Ahmad 한국식품과학회 2005 Food Science and Biotechnology Vol.14 No.2

Compound-specific δ¹³C and δ¹⁵N analyses of fatty acids and amino acids for discrimination of organic, pesticide-free, and conventional rice (<i>Oryza sativa</i> L.)

Chung, Ill-Min,Kim, Jae-Kwang,An, Yeon-Ju,Kwon, Chang,Kim, So-Yeon,Yang, Yu-Jin,Yarnes, Christopher T.,Chi, Hee-Youn,Kim, Seung-Hyun Elsevier 2019 Food chemistry Vol.283 No.-

<P><B>Abstract</B></P> <P>Herein, we improve the procedure for organic rice authentication using compound-specific δ<SUP>13</SUP>C and δ<SUP>15</SUP>N analyses of fatty acids and amino acids, addressing the increasing demand for accurate methods to confirm organic authenticity. Organic rice (OR) and pesticide-free rice (PFR) featured higher values of δ<SUP>15</SUP>N<SUB>bulk</SUB> than conventional rice (CR), whereas the corresponding differences between OR and PFR were insignificant. Additionally, OR, PFR, and CR could be discriminated based on some δ<SUP>13</SUP>C<SUB>amino-acid</SUB> and δ<SUP>15</SUP>N<SUB>amino-acid</SUB> values. δ<SUP>13</SUP>C<SUB>bulk</SUB> was correlated with most δ<SUP>13</SUP>C<SUB>fatty-acid</SUB> (<I>r</I> ≥ 0.596) values, and δ<SUP>15</SUP>N<SUB>bulk</SUB> was strongly correlated with most δ<SUP>15</SUP>N<SUB>amino-acid</SUB> (<I>r</I> ≥ 0.834) values. The first component in the orthogonal projection to latent structure-discriminant analysis model allowed for a clear separation between OR and PFR, and good predictability (<I>Q</I> <SUP>2</SUP> <I>Y</I> = 0.506). Thus, the present study improves the reliability of organic authentication when bulk stable isotope ratio analysis alone is insufficient for the accurate discrimination of OR, PFR, and CR.</P> <P><B>Highlights</B></P> <P> <UL> <LI> This work focuses on the detection of incorrect or fraudulent organic rice labeling. </LI> <LI> Compound-specific isotope analysis was employed for organic rice authentication. </LI> <LI> Best results were achieved by OPLS-DA combined with δ<SUP>13</SUP>C/δ<SUP>15</SUP>N amino acid analysis. </LI> <LI> δ<SUP>13</SUP>C<SUB>lysine</SUB> was identified as the greatest contributor (VIP > 1) for all OPLS-DA models. </LI> </UL> </P>

Comparison of isoflavones in soybean (Glycine max (L.) Merill) germplasms by different origins

Ill-Min Chung,Eun-Hye Kim,Ju-Jin Lim,Bo-Ra Yu,Ye-Seul Yang,Jae Yeon Yoon,Joo-Hyun Lee,Seung-Hyun Kim 한국육종학회 2013 한국육종학회 심포지엄 Vol.2013 No.07

Soybean [Glycine max (L.) Merrill] is one of the world’s most major crops as not only an important source of oil and protein, but also secondary metabolites. Intake of soybean is associated with decreased risk of cardiovascular disease and osteoporosis, as well as cancer, including breast and colon cancers. Seventy soybeans germplasms collected from 4 different countries, America (6 varieties), China (15 varieties), Japan (16 varieties), and Korea (33 varieties), were distributed by Chungbuk National University (Cheongju, Chungbuk, Korea) and cultivated in Konkuk University farm. This study investigated the isoflavones in seventy soybeans according to 4 different origins (America, China, Japan and Korea). Between 4 different origins, Korea showed highest concentrations of total isoflavones (1292.6 ± 438.6 ㎍ g-1) and China showed the lowest concentrations of total isoflavones (843.8 ± 365.7 ㎍ g-1). The total isoflavone contents in soybean of America and China ranged from 572.3 ㎍ g-1 to 2001.9 ㎍ g-1 and from 275.8 ㎍ g-1 to 1521.8 ㎍ g-1, respectively. And the isoflavone contents of Japan and Korea ranged from 473.3 ㎍ g-1 to 2314.6 ㎍ g-1 and from 419.0 ㎍ g-1 to 3010.7 ㎍ g-1, respectively. Malonylgenistin (356.9 ± 158.8 ㎍ g-1) was the major isoflavones among 12 isoflavones. Specially, glycoside and malonylglycosides constituted 49.2 % and 45.3 % of total isoflavones in soybeans, respectively.

Varietal Variation in Antioxidative Activity of Rice Grain by DPPH and TBA Methods

Ill Min Chung,Kwang Ho Kim,Joung Kuk Ahn,Jin Ohk Lee 韓國作物學會 2000 Korean journal of crop science Vol.45 No.4

This study was to investigate antioxidative activity of rice grain using 1, 1-Diphenyl-2-picrylhydrazyl(DPPH) and thiobarbituric acid (TBA) method and germination ability for screening rice varieties with high antioxidative activities on korean native and foreign rice varieties harvested in 1998 and 1999. The average antioxidative activity of foreign rice varieties (DPPH 63.5% and TBA 55.2%) was significantly higher than that of native rice varieties (DPPH 47.2% and TBA 45.6%) on varieties harvested in 1999. The promptness index (PI) of native rice varieties was higher in stored rices for three months (mean PI=160.7) than that of stored rices for a year (mean PI=141.6). On the other hand, the PI of foreign rice varieties was higher in stored rices stored for a year (mean PI=176.7) than that of stored rices for three months (mean PI=157.5). Varieties with high redness of hulled rice (a-value) showed significant lipid peroxidation inhibitory activity to DPPH in a stored rices for a year (r=0.5744** ) and stored rices for three months (r=0.5630** ) . These results indicate that the pigments of hulled rice varieties may play important antioxidative roles and colored rice varieties with higher antioxidative potentials can be developed and also may provide information with rice breeder to breed rice variety with a high antioxidative activity for a rapid screening of a small amounts of a large number of samples using color value.

Variation of isoflavones in soybeans according to different cultivation regions and seeding periods

Ill-Min Chung,Eun-Hye Kim,Min-Young Kim,Jong-Hyun Lim 한국육종학회 2012 한국육종학회 심포지엄 Vol.2012 No.07

The present study is designed to know the effect of cultivative environment and conditions, locational genetic variation on the content and components of seed isoflavones soybean genotype. The concentration of isoflavones showed difference between three different seeding time. Specially, Somyung of normal seeding (6. 21) revealed the highest concentrations (7657.0 ㎍ g-1) than other soybean seeds. On the other hand, Cheongjakong and Taekwangkong of early seeding (5. 25) showed the lower concentrations (1518.6 ㎍ g-1, 2524.0 ㎍ g-1) than other soybeans. For different regions, Taekwangkong of Yeoju region had the highest total isoflavone concentrations (3227.5㎍ g-1) than other cultivation regions. While, Taekwangkong of Miryang region (619.5 ㎍ g-1) revealed lower concentrations than other regions. And, Cheongjakong of Yeoju regions also revealed higher concentrations (3140.1 ㎍ g-1) than other regions. On the other hand, Cheongjakong of Miryang (1619.4 ㎍ g-1) showed lower concentrations of total isoflavone concentrations. In our present study, the isoflavone concentration of soybean was influenced by seeding and cultivation regions.

Investigation of the quality and stability in soybean flour (Glycine max L.) according to the storage conditions

Ill-Min Chung,Sung-Kyu Park,Min-Jeong An,Ji-Hee Lee,Kyoung-Jin Lee,Na-Young Son,Yeon-Ju An,Seung-Hyun Kim,Koan-Sik Woo 한국작물학회 2017 한국작물학회 학술발표대회 논문집 Vol.2017 No.-