8-hydroxyguanine이 전립선비대증 발생에 미치는 효과

이재원,이석영,이형래,이상철,김용태,김원재 충북대학교 의학연구소 2002 忠北醫大學術誌 Vol.12 No.2

연구목적: 전립선비대증은 노화현상에 의해 발생하는 질환으로서 임상적으로 주로 50세 이상에서 증상이 나타난다. 산소 라디칼은 DNA의 염기에 다양한 손상을 미칠 수 있다. 산소 라디칼에 의한 DNA의 변화에는 여러 가지 형태가 있으며 이러한 변화들 가운데서 guanine이 8-oxoguanine (8-hydroxyguanine, oh8Gua)으로의 수산화(hydroxylation) 반응은 가장 흔히 관찰되는 형태로서 노화 및 만성 퇴행성 질환, 기타 여러 질환의 중요한 병인으로 인식되고 있다. 세포에 유해한 영향을 주는 oh8Gua의 생성으로부터 유전자를 보호하는 수복효소 중 8-oxoguanine-DNA glycosylase (OGG1)가 주된 역할을 하는 것으로 알려져 있는데 이 효소를 생산하는 유전자인 hOGG1은 사람마다 유전자형이 다르다. 따라서 본 연구에서는 전립선비대증이 노화와 관련된 hOGG1 유전자형에 따라서 발생에 차이가 있는지를 조사하였다. 대상 및 방법: 충북대학교 병원에서 전립선비대증으로 진단 받고 입원하여 경요도전립선절제술을 받은 환자로 병리 조직학적으로 전립선비대증이 확인된 81명을 환자군으로 하였으며, 충청북도내 10개 군에서 50세 이상의 건강한 성인 남자 764명을 대상으로 실시한 전립선 무료검진에서 경직장 초음파 검사상 전립선 크기 20 gm 이하, 국제 전립선 증상 점수(IPSS) 7점 이하, 요속 10 ㎖/s 이상, 전립선 특이 항원(PSA) 4.0 ng/㎖ 이하인 81명을 대조군으로 설정하였다. 이들에서 채취한 혈액에서 genomic DNA를 얻은 후 PCR-SSCP (polymerase chain reaction-single stranded conformation polymorphism), 직접 염기서열분석(direct DNA sequencing) 및 RFLP (restriction fragment length polymorphism)를 통해서 두 군 사이의 hOGG1 유전자의 다형성을 분석하였다. 결과: 대조군에서 codon 326형은 Ser326Ser형이 12례(14.8%), Ser326Cys형이 51례(63.0%), Cys326Cys형이 18례(22.2%)였으며 전립선비대증 환자군에서는 Ser326Ser형이 15례(18.5%), Ser326Cys형이 34례(42.0%), Cys326Cys형이 32례(39.5%)로 두 군 사이에 통계적인 유의성이 관찰되었다(p=0.022). 또한 Cys326Cys형과 나머지(Ser326Ser, Ser326Cys)형으로 나누어 시행한 통계 분석에서도 대조군에서는 각각 18례(22.2%), 63례(77.8%)였으며 전립선비대증 환자군에서는 32례(39.5%), 49례(60.5%)로 통계적인 유의성이 관찰되었다(p=0.017, OR=2.286, 95% CI=1.149-4.546). 결론: hOGG1 유전자의 형태와 전립선비대증의 발생과는 밀접한 관련이 있었다. 즉 hOGG1 Cys326Cys형을 가진 사람에서 전립선비대증이 발생한 가능성이 높을 것으로 사료된다. Purpose: 8-oxoguanine DNA glycosylase (OSS1) rapairs DNA by removing 8-oxoguanine (oh8Gua), a highly mutagenic oxidative DNA adduct. Recently, the human gene for OGG1 (hOGG1) was cloned and several genotypes have been reported. However, the implications of such genotypes in benign prostatic lyperplasia have not been demonstrated. To assess the involvement of hOGG1 associated with the aging process on the developing of BPH, we analyzed the genetic polymorphisms of hOGG1. Materials and Methods: We studied the hOGG1 gene polymorphisms in 81 cases of BPH and 81 cases of normal controls. We performed the SSCP, PCR-based restriction fragment length polymorphism (RFLP) and direct sequencing to characterize the genetic polymorphisms of hOGG1 in both BPH patients and controls. Results: We found two polymorphic sites. A serine326/cysteine polymorphism at codon 326 (1a type) in exon 7 was associated with an exchange of amino acid. Another polymorphic site at codon 324 (1b type) in exon 6 was silent. We found that the distribution of hOGG1 genotypes in BPH patients (Ser326Ser type 18.5%, Ser326Cys type 42.0% and Cys326Cys type 39.5%) was significantly different from that in controls (14.8%, 63.0% and 22.2%, respectively) (p=0.022). Homozygosity for the Cys326Cys genotype significantly increased the risk of developing BPH, with the odds ratio (OR) being 2.286 (95% confidence interval [CI]=1.149-4.546) Conclusion: Our results suggest that the hOGG1 Cys326Cys genotype might play a important role in the development of BPH.

온열요법과 기치유요법이 어깨근통증 압통역치에 미치는 영향

이석복 ( Lee Seok Bok ),이민선 ( Lee Min Sun ),이형환 ( Hyung H Lee1 ) 한국자연치유학회 2014 Journal of Naturopathy Vol.3 No.1

In this study, we wanted to understand the influence of thermotherapy, Qi-therapy, and their combination therapy on the oppressive muscular pains in shoulders. For the clinical study, 102 subjects were divided into 3 groups of thermotherapy, Qi-therapy, and the combination therapy to see the differences in the therapeutic effects. The treatments were taken 5 times for 2 weeks in 3-day intervals and pre- and post-treatment assessments were undertaken for the visual pain assessment (VAS, visual analogue scale) and experimental pressure threshold in 8 points in the relevant muscles (PPT). VAS score was decreased by 39% after thermotherapy, 66.5% after Qi-therapy, and 72.3% after combination therapy, all with significance (p<.05). The experimental pressure thresholds of shoulder muscles, the upper trapezius muscles, the lower trapezius muscles, the anterior trapezius muscles. the supraspinous muscles, the greater pectoral muscles, and the sternocleidomastoid muscles were increased by 4.3~11.3% after thermotherapy, 5.9~19.9% after Qi-therapy, and 9.3~25.4% after combination therapy. The experimental pressure thresholds in the 8 points in the relevant muscles did not show a significant difference between the Qi-therapy and combination therapy in the upper and lower trapezius muscles, however, did show a significant difference to the thermotherapy. In the shoulder muscles, anterior and posterior trapezius muscles, supraspinous muscles, supraspinous muscles, greater pectoral muscles, and sternocleidomastoid muscles, there were significant superiorities in Qi-therapy over thermo therapy, and in the combination therapy over Qi-therapy. In conclusion, it could be understood that both thermotherapy and Qi-therapy have been effective in the improvement of pains, and especially combination therapy showed better effectiveness. It is expected to apply these natural therapies to the pain treatment of human body to contribute to the improvement of health and welfare.

전기 기계적 해리로부터 회복된 쯔쯔가무시병에서의 급성 전격성 심근염

정영학,이민주,이경화,이지훈,김석형,이병권 이화여자대학교 의과학연구소 2016 EMJ (Ewha medical journal) Vol.39 No.1

Scrub typhus, caused by Orientia tsutsugamushi , is an acute febrile illness. Characteristics of tsutsugamushi disease are fever, rash and eschar. However, severe complications might rarely occur, such as acute fulminant myocarditis caused by scrub typhus. Thus, there are few reports of recovery from seriously complicated cases. We report on an adult male with scrub typhus complicated with acute fulminant myocarditis with no previous comorbid illness who recovered successfully with proper treatment including antibiotics, ventilator support, percutaneous cardiopulmonary support, and continuous renal replacement therapy.

서울의 Penicillinase Producing Neisseria Gonorrhoeae 발생빈도(1996)

김재홍,황동규,전재홍,김윤석,김중환,김용준,이창균,임동진,김현수,조창근,김경문,박상훈,전우형,김희성,이호정,차명수,김갑형,김형석,김석우,황지환,박병순,권오상,이민수,송기훈,성소영,이인섭,부태성 대한화학요법학회 1999 대한화학요법학회지 Vol.17 No.2

Background : In recent years, gonorrhea has been panedemic and remains one of the most commom STDs in the world, especially in developing countries. Objective & Methods: For the detection of a more effective therapeutic regimen and assessing the prevalence of PPNG, we have been trying to study the patients who have visited the VD Clinic of Choong-Ku Public Health Center in Seoul since 1980 by means of the chromogenic cephalosporin method. Results: In 1996, 139 strains of N. gonorrhoeae were isolated, among which 53(39.0%) were PPNG. Conclusion: Our results suggests that after a peak of 74.3% in 1993, the prevalence of PPNG in Seoul is gradually declining.

자성선숙어(protogynous fish)의 성전환

이정재,김형배,박인석,고환봉,이영돈 濟州大學校海洋硏究所 1993 해양과환경연구소 연구논문집 Vol.17 No.-

제주 연근해에 서식하는 자성선숙형 자웅동체어인 놀래기류와 능성이류를 대상으로 성전환에 따른 생식소의 조직학적 변화와 체색의 변화 등의 성적이형 현상을 토대로 하여 성전환 특성을 고찰하였다. 1. 용치놀래기, 어렝놀래기, 놀래기는 6월과 7월에 주산란을 하는 하계산란종인 반면에 황놀래기는 10월과 11월에 산란하는 추계산란종이였다. 2. 성전환 체장범위는 용치놀리기 13. 0-15. 0㎝, 황놀래기는 11.5-16.0㎝, 그리고 놀래기 전장 10.0-13.0㎝였다. 3. 성전환은 주로 산란기 전후로 하여 나타나고 있다. 용치놀래기, 어렝놀래기, 놀래기는 1차웅과 2차웅이 혼재하고 잇으나 어렝놀래기는 2차웅만이 출현하였다. 4. 성전환에 따른 용치놀래기, 어렝놀래기, 황놀래기 체색변화는 선홍색 또는 선황색 계통에서 색조가 다양한 녹색이나 황색계통으로 전환되고 있다. 놀래기의 경우 전반적의 체색의 변화는 미미하나 몸 가운데에 파란반점과 가로줄무늬를 띠고 있다. 어렝놀래기 경우 체색의 변화와 함께 등지느러미 제1, 2극의 돌기가 신장되고 있다. 5. 성전환 과정에 따른 놀래기류의 생식소내 조직학적 변화는 난모세포의 퇴행변성에 따라 다양한 간질세포와 혈관의 발달을 보였다. 이후 난소의 박판주위에서 정원세포와 정모세포들이 출현하기 시작하여 기능적 정소로 전환되고 있다. 6. 자바리는 전장 30.0-92.3㎝, 능성어는 전장 23.4-38.7㎝에서 생식소내 주변인기 난모세포를 갖는 암컷이었고, 붉바리는 전장 22.0-33.0㎝에서 양성생식소를 갖는 개들도이 출현하고 있다. Studies and reviews have been based on sex reversal diagnosis of histological change and body color change by sexual dimorphism on protogynous hermaphrodite fish, Labridae, Serranidae inhabited at the costal area of Cheju-do, Korea. Halichoreres poealopterus, Pteragogus flagellifera and H, tenuispinis were summer season, spawing species which mainly spawn in Jun and July, but Pseudolabrus japonicus was autumn season spawing species which mainly spawn in October and November. The standard length range of sex reversal were 13. 0-15. 0㎝ H, poecuilopterus, 9. 5-15.0㎝ P,flagellifer, 11. 5-16 0㎝ P, japonicus, and 10. 0-13 0㎝ (total length) H, tenuispinis respectively. Sex reversal mainly appeared before and after spawing season. H. Poecilopterus, H. tenuispinis and P. japonicus were composed of monandry and diantry, but P. flagellifera was diandry. By sex reversal, the body colors of Ho poecilopterus, P. flagellifera and P. japonicus change from pale red or yellow lines to brilliant green or dark yellow lines. The body colors of H. tenuispinis change slight, but containe lateral stripe pattern and blue spot in body center. In P.flagellifera, 1st, 2nd spine process of dosal fin were grown with body colour change. In sex reversal process, histological change of Labridae of inner gonad showed development of various interstitial cells and blood vessel by degeneration of oocyte. Thereafter, spermatogonia and spermatocyte begin to appear at the inner edge regions of the ovarian lamellae and then converted from ovary to functional testis. Epinephelus moara of 30. 0-92.3㎝ in TL, E.Septenfasciatus of 23. 4-38.7㎝ in TL were female with contained oocyte of perinucleolus stage. E. akaara of 22.0-33.0㎝ in TL appeared individuals of ovotestis.

캠퍼스 인트라넷 사용자 만족요인 연구

이형석,박승봉,한재민 한국인터넷비즈니스학회 2003 인터넷비지니스연구 Vol.4 No.2

본 연구는 최근 모든 대학들이 서비스를 제공하고 있는 캠퍼스 인트라넷에 대한 대학생들의 만족도를 분석하였다. 기존의 기업 정보시스템 평가에서 사용되어지고 있는 정보 요인, 시스템 요인 그리고 개인 요인들이 전반적인 사용자 만족에 어떠한 영향을 미치는지를 살펴보았다. 분석결과 정보 요인에서는 내용성과 접근성이 유의한 요인으로 개인 요인에서는 사용자 개인의 능력과 흥미가 유의하게 나타났다. 그리고 시스템 요인에서는 인트라넷 사용의 용이성과 사용자 인터페이스가 전반적인 사용자 만족에 영향을 미치는 요인으로 나타났다. 따라서 본 연구의 캠퍼스 인트라넷에 대한 만족 요인 분석결과를 토대로 효과적인 대학정보서비스 제공과 지속적인 수정 및 업데이트를 위한 정보를 제공할 수 있을 것으로 본다. This study analyzed that university students' satisfaction with campus Intranet provided by all of university in recent. In detail, this showed how information, system and individual factor being used in evaluation on corporate information system are affecting the overall user satisfaction. The result of the analysis shows that information content and information access in information factors, individual ability and interest in individual factors were significant factor. And easiness of Intranet use and user based interface in system factors affected significantly user satisfaction. Thus, this study provides the information for continuous correction and update as well as educational information service effectively.

면역학적 접근법을 이용한 미소대장암의 진단 및 외과적 치료

이기형,이석환,고석환 경희대학교 1997 慶熙醫學 Vol.13 No.1

충남 서북부지역 여성의 질세포진 검사에 대한 임상적 고찰

이석민,선우재근,장경택,서수형,김민관,배동한 순천향의학연구소 1996 Journal of Soonchunhyang Medical Science Vol.2 No.2

Pap smear were performed to 30,294 women living in the north-west of Chung-nam. The result are summarized below 1. Positive vaginal cytology was 1.09 percent. 2. 72 percents women in their 30's and 40's experienced vaginal cytology. 3. Vaginal cytology was performed at a decreasing rate as age increases. 4. Positive cytology rate is 2.42% higher than average for elderly women over the age of 65. The present date indicates that pap smear is being widely recognized to be important but they needs to be continued advocacy for pap smear.

사회체육지도자들의 Stress에 관한 연구

이종희,김정석,윤형기 경희대학교 체육과학연구소 1997 體育學論文集 Vol.25 No.-

We tried questionare survey to 331 physical director who worked for sports in the region of Seoul and Kyung-Gi Do by scientific analysis of those result. we got some conclusion the difference of psychological and physical Stress. 1. Non-complex group revealed more Stress than complex group in psychological and physical aspect. The comparative analysis showed nonsignificant difference. 2. Male group revealed more Stress than female group in Psychological aspect. and Female group revealed more Stress than male group in physical aspects. The comparative analysis showed signiticant difference(p<.GO 1). 3. Graduate School graduated group revealed more Stress than college graduated group, university graduated group in psychological aspect. The comparative analysis showed significant difference(p<.05). 4. Over 25 house per week group revealed more Stress than 20-24 hours par week. less group below 19 hours per week in psychological aspect. the comparative analysis showed significant difference(p<.05).

Si₃N₄/SiC 복합체의 기계적 성질에 미치는 Y₂O₃-Al₂O₃계 소결조제의 영향

이홍림,서유석,이형직 연세대학교 산업기술연구소 1992 논문집 Vol.24 No.2

Si₃N₄/SiC composites were prepared by mixing Si₃N₄powder with SiC powders of 0, 10, 20, 30 vol%. To these composites were added the powders of Y₂O₃-Al₂O₃system as sintering aids. These samples were hot-pressed at 1850℃ for 60min under a pressure of 30 MPa in N₂. Some of the samples were annealed at 1400℃ for 4 hr under N₂ before the measurement of mechanical properties. β-Si₃N₄ phase is starting powder was all transformed to β-Sic phase remained unchanged. The fracture toughness of the composites increased more or less with the increase of SiC content. The room temperature strength decrease was observed around 10-30 vol% of SiC content. For the sample in which grain boundary crystallization occurred after annealing, fracture toughness increased while roon-temperature strength decreased.