간 세포암에서 VEGF, TGF-β1, b-FGF 발현의 의의

김성용,남충현,주종우,채만규,백무준,이문수,김형철,안현철,김홍수,김창진,김창호 순천향의학연구소 2001 Journal of Soonchunhyang Medical Science Vol.7 No.1

Purpose: Angiogenesis is important for the proliferation and the metastasis of solid tumors. The growth of a solid tumor is widely recognized to depend on the process of neovascularrozation. Without angiogenesis, tumors cease to grow beyond even a few milimeters in diameter. It has been shown that tumor vascular density is an independent prognostic marker in several types of human tumors and is known to correlate with poor prognosis. To date, many angiogenic factors have been identified, such as transforming growth factor-α(TGF-α), transforming growth factor-β(TGF-β), fibroblast growth factor family(FGF), vascular endothelial growth factor(VEGF), platelet derived endothelial cell growth factor(PD-ECGF), tumor necrosis factor-α(TNF-α), and angiogenin. Hepatocellular carcinoma(HCC) is the second most common tumor in Korean males and is known as a typical hypervascular tumor with frequent portal vein invastion. The authors identified the expreesion of VEGF, TGF-β1, and b-FGF in HCC specimens and evaluated the relationship between these growth factors and the clinicopathologic characteristics of HCC. Method: We reviewed the medical records of 30 patients who were diagnosed as hepatocellular carinoma treated with hepatic resection between January 1994 and December 1998 in Soonchunhyang University Chunan Hospital. The selection of the cases was decided according to the condition of paraffin block fixation. The prognostic factors such as age, sex, tumor size, concentration of serum α-fetoprotein, presence of liver cirrhosis, presence of tumor emboli in portal vein, TMN stage, amount of transfusion during the operation, hepatitis B virus(HBV) infection, and Edmonson-Steiner(E-S) grade were investigated. Relationship between the prognostic factors and the immunopathologic expression of the TGF-β1, b-FGF, and VEGF was examined. Result: Thirty patients (24 males, 6 females) were included in the current study. The patient's mean age was 50.6 years and the age ranged from 36 to 65 years. The mean size of the tumor was found to be 5.2cm. All the patients were follewed up for 7 to 63 months. Child's classification A patients were 23(76.7%)cases, B patients were 7(23.3%)cases, and C was none. Immunohistochemical staining of HCC tumor mass in VEGF expression patients were 17(56.7%), b-FGF expression patients were 10(33.3%), and TGF-β1 expression patients were 10(33.3%). VEGF expression or more than one positive expression among the three factors correlated with tumor size and the stage of HCC but did not correlated with other clinicopathological characteristics. TGF-β1 and b-FGF did not correlate with any clinicopathological characteristics. Conclusion: The results suggest that the expression of VEGF or more than one positive expression among the three factors in HCC cells may be a significant prognostic factor of HCC.

Mirizzi 증후군의 변형된 분류와 치료

김형철,강길호,채만규,김성용,백무준,이문수,박상흠,이문호,김창호,송옥평,조무식,박희주 순천향의학연구소;Soonchunhyang Medical Research Institute 2000 Journal of Soonchunhyang Medical Science Vol.6 No.1

Purpose : The Mirizzi syndrome is relatively rare and preoperative diagnosis of this disease is difficult. In 1978, Morelli suggested the subclassification of the Mirizzi syndrome into acute or chronic form. We experienced 5 cases of acute form. We analysed clinical features, preoperative radiologic findings and operative findings of 18 cases including acute forms which were diagnosed as Mirizzi syndrome and should suggest the modified classification of Mirizzi syndrome for choice of appropriate treatment. Method : From January 1995 to December 1998, 18 cases, of which 8 cases were diagnosed at Soonchunhyang University Chunan Hospital, and 10 cases were reported in the Korean Journal were retrospectively analysed with regard to clinical features, preoperative radiologic findings and operative findings. According to the clinical features, whole cases were divided into type Ⅰ(acute form) and type Ⅱ(chronic form) and then each type of cases were subclassified according to preoperative radiologic findings and operative findings. Results : Of 18 cases there were 5 cases in type Ⅰ(27.8%), 13 cases in type Ⅱ(72.2%). Type Ⅱb was most common. Type Ⅰa cases were treated only with cholecystectomy. We applied cholecystectomy, T-tube choledochostomy and patch technique in type Ⅰb and thpe Ⅰc cases. Cholectystectomies including removal of gallstones and internal drainage procedures were done in type Ⅱ chronic forms. Conclusion : The acute form(Type Ⅰ) of Mirizzi syndrome was suggested by Morelli might be subclassified into typeⅠa,Ⅰb and Ⅰc following the presence of the necrotic defect in common hepatic duct. Through the modified classification of Mirizzi syndrome based on clinical feature, preoperative radiologic findings and operative findings, we can choice appropriate treatment.

Newcastle Disease Virus의 Fusion 단백 유전자의 제한효소분절형태(RELP)의 특이성

장경수,김지영,김석,김태용,송영환,허지연,김현수,송희종,전무형 충남대학교 수의과대학 동물의과학연구소 2001 動物醫科學硏究誌 Vol.9 No.-

To evaluate the specificity of restriction fragment length polymorphism (RFLP) of Newcastle disease virus (NDV) fusion (F) protein gene for various NDV strains. F protein genes of NDV CBP-1 isolate, LaSota, B1 and Kyojungwon (KJW) were amplified by reverse transcriptase-polymerase chain reaction (RT-PCR) and RFLP were examined. In RT-PCR using F primer sets for 1.7 kb F gene, the amplified products were observed in CBP-1, LaSota and B1 except KJW. However, F2 primer set for 521 bp yielded the amplified bands with all strains. The amplified F genes (1.7 kbp) were treatd with 15 different restriction enzymes to analyze RFLP and the cleavage patterns. CBP-1 showed various cleavage patterns with 8 enzymes, LaSota, with 11 enzymes and B1, with 11 enzymes. According to the cleavage patterns, the physical maps were constructed, and it was found that CBP-1, LaSota and B1 strains had specific cleavage sites with HinfI, XbaI and XhoI. These findings suggest that RFLP are worth applying in the diagnosis and molecular epidemiological studies of Newcastle disease.

Brucella abortus 국내 분리주의 36kDa 외막단백을 암호하는 omp2b 유전자의 염기 분석

허지연,김지영,송근호,김명철,박창식,김태용,김정화,전무형 한국수의공중보건학회 2003 예방수의학회지 Vol.27 No.1

B. abortus 국내 분리주의 분자유전학적 성상을 규명하고 분자역학적 연구에 필요한 기초자료를 얻기위해 36kDa OMP를 암호하는 omp2b 유전자를 PCR법으로 확인하고, 그리고 omp2b 유전자의 핵염기 서열과 아미노산 서열을 결정하고 이 결과를 Brucella 표준균주 및 여러 동물에서 분리된 Brucella 분리주와 상호 비교 분석하였던 바 다음과 같은 결과를 얻었다 1. B. abortus 분리주 (7주)에 대해 omp2b primers를 이용하여 PCR을 수행 한 바 공시한 참조균주와 분리균주 모두에서 1,251bp의 특이한 DNA 절편이 각각 증폭되었다 2. omp2b 유전자의 1,089bp에 대한 염기서열과 이에 대한 362개의 아미노산 서열을 결정하였다. 분리균주의 omp2b 유전자의 염기서열을 참조균주와 비교 분석한 결과 B. abortus 2308 (biovar 1) 및 B. abortus B3196 (biovar 5)과는 99.6%~l00% 및99.5%~99.9%의 매우 높은 상품성을 나타내었고, B. abortus 45/20 (biovar 1)과는 97.1%~97.4%의 상품성을 나타내었다. 또한 362개의 아미노산 서열에 대해 분석하였던 바, 분리균주들은 B. abortustus 2308 (biovar 1) 및 B. abortus B3196 (biovar 5)과는 99.2%~l00%의 매우 높은 상품성을 보였으며, B. abortus 45/20 (biovar 1)과는 96.4%~97.2%의 상품성을 보였다. 3. 본 시험에 공시된 브루셀라균주의 omp2b 유전자서열과 여러 동물에서 분리된 브루셀라 분리주의자료를 바탕으로 phylogenetic tree를 작성 한 바, 국내 분리주는 B. abortus 2308 (biovar 1), B. abortus B3196 (blovar 5), B. abortus 45/20 (biovar 1), B. suis 1330 (biovar 1), B. melitensis 16M (biovar 1), B. canis NCTC 01854, B. neotomae 5k33과 97.3%이상의 높은 근연성을 보이며 동일한 cluster에 소속되었으며, omp2b 유전자는 매우 잘 보존됨을 알 수 있었다. omp2b gene of Brucella abortus isolates encoding 36kDa outer membrane protein (OW) was amplified by polymerase chain reaction (PCR) and the sequences of nucleotide and amino acid were determined and analyzed to obtain the basic data for molecular epidemiological features of brucellosis in Korea. In PCR using omp2b primers for the gene of 36kDa OMP, the specific signals at 1,251bp were demonstrated, The nucleotide sequences of omp2b gene of 1,089bp were sequenced by dye terminator cycle sequencing method, and 362 amino acid sequences were predicted. As compared the nucleotide sequences with those of B. abortus reference strains, the isolates showed the very high homology to the reference strains ranging 97.1%-100%. The similar results were also obtained in the analysis of the amino acid sequences. In the phylogenetic tree, the isolates clustered with the reference strains and the majority of strains originated from pig, dog, wood rat and cattle.

Brucella abortus 국내 분리주의 36kDa 외막단백을 암호하는 omp2b 유전자의 염기 분석

허지연,김지영,송근호,김명철,박창식,김태용,김정화,전무형 충남대학교 형질전환복제돼지연구센터 2004 논문집 Vol. No.8

B. abortus 국내 분리주의 분자유전학적 성상을 규명하고 분자역학적 연구에 필요한 기초자료를 얻기 위해 36kDa OMP를 암호하는 omp2b 유전자를 PCR법으로 확인하고,그리고 omp2b 유전자의 핵염기 서열과 아미노산 서열을 결정하고 이 결과를 Brucella 표준균주 및 여러 동물에서 분리된 Brucella 분리주와 상호 비교 분석하였던 바 다음과 같은 결과를 얻었다. 1. B. abortus 분리 주 (7주)에 대해 omp2b primers를 이용하여 PCR을 수행한 바 공시한 참조균주와 분 리균주 모두에서 1,251bp의 특이한 DNA 절편이 각각 증폭되었다. 2. omp2b 유전자의 1,089bp에 대한 염기서열과 이에 대한 362개의 아미노산 서열을 결정하였다. 분리 균주의 omp2b 유전자의 염기서열을 참조균주와 비교 분석한 결과 B. abortus 2308 (biovar 1) 및 B abortus B3196 (biovar 5)과는 99.6%∼100% 및 99.5%∼99.9%의 매우 높은 상동성을 나타내었고,B. abortus 45/20 (biovar 1)과는 97.1%~97.4%의 상동성을 나타내었다. 또한 362개의 아미노산 서열에 대해 분석하였던 바,분리균주들은 B.abortustus 2308 (biovar 1) 및 B. abortus B3196 (biovar 5)과는 99.2%∼100%의 매우 높은 상동성을 보였으며,B. abortus 45/20 (biovar 1)과는 96.4%∼ 97.2%의 상동성을 보였다. 3. 본 시험에 공사된 브루셈라균주의 omp2b 유전자 서열과 여러 동물에서 분리된 브루셀라 분리주의 자료를 바탕으로 phylogenetic tree를 작성 한 바,국내 분리주는 B. abortus 2308 (biovar 1),B. abortus B3196 (biovar 5),B. abortus 45/20 (biovar 1),B. suis 1330 (biovar 1),B. melitensis 16M (biovar 1),B. canis NCTC 01854,B. neotomae 5K33과 97.3% 이상의 높은 근연성을 보이며 동일한 cluster에 소속되었으며,omp2b 유전자는 매우 잘 보존됨을 알 수 있었다. omp2b gene of Brucella abortus isolates encoding 36kDa outer membrane protein (OMP) was amplified by polymerase chain reaction (PCR) and the sequences of nucleotide and amino acid were determined and analyzed to obtain the basic data for molecular epidemiological features of brucellosis in Korea. In PCR using omp2b primers for the gene of 36kDa aMP, the specific signals at 1,251bp were demonstrated, The nucleotide sequences of omp2b gene of 1,089bp were sequenced by dye terminator cycle sequencing method, and 362 amino acid sequences were predicted. As compared the nucleotide sequences with those of B. abortus reference strains, the isolates showed the very high homology to the reference strains ranging 97.1%∼100%. The similar results were also obtained in the analysis of the amino acid sequences. In the phylogenetic tree, the isolates clustered with the reference strains and the majority of strains originated from pig, dog, wood rat and cattle.

후방골절단술과 Skeletal anchorage system를 이용한 사악구치부압하 : 증례보고 CASES REPORT

임광수,풍무걸,김기형,이희철,김우형,김동우 대한악안면성형재건외과학회 2002 Maxillofacial Plastic Reconstructive Surgery Vol.24 No.1

The severe extrusion of the maxillary [post.teeth after the early loss of the mandibular post.teeth makes the fabrication of the mandibular prothesis difficult, and without proper planning.the treatment results are often unsatisfactoy. Several treatment modalitys have been introducted to correct the interarch space deficency.:Reduction the occlusal surfaces,Crown lengthening,Endodontics & restorative approach,Extraction, Orthodontic intrusion and segmental osteotomy. Because of many advantages,conservative method is widely used if it has no particular limits of treatment adjustment, but the cases of surgical correction indication are found in clinical experience. In our clinic,we corrected severe extruded maxillary post.teeth by performance of a unilateral posterior osteotomy with superior repositioning of the segment and orthodontic intrusion using Skeletal anchorage system,so here we reported the cases with a study of documents.

Newcastle Disease Virus의 F와 HN 유전자 재조합 DNA 제조 및 면역원성

김지영,초가기,박종현,서상희,박창식,김명철,전무형 충남대학교 형질전환복제돼지연구센터 2007 논문집 Vol. No.10

수의 임상에서 침술마취의 적용

김명철,전무형,박창식 충남대학교 형질전환복제돼지연구센터 2004 논문집 Vol. No.8

Acupuncture is used to treat pathological conditions and to prevent pain from arising. The pathological conditions that can be treated can be painless or painful conditions. Pain modification is an important part of traditional acupuncture therapies. Acupuncture analgesia (AA) is a specific form of acupuncture; the stimulation of the acupuncture points must be strong and long (about 15 to 30 minutes) before a local or general analgesia or hypalgesia will develop. Development of modern AA began in the late fifties. Effectiveness of acupuncture in alleviation of pain was quickly noticed and brought to the medical researchers and surgeons. Through their diligent pursuit. AA has been established as a viable alternative for relief of pain and substitution of conventional anesthesia. In veterinary medicine, early successful applications of AA to surgery of domestic animals were reported in the late sixties. Local or regional anesthesia is often preferred to general anesthesia in cattle. Acupuncture was effective in inducing analgesia. Regional electroacupuncture analgesia is potentially useful for standing surgeries in cattle. Some standing surgeries may be easily accomplished using electroacupuncture anesthesia. The major advantages of AA or EAA are its safety in high-risk patients and excellent post-operative pain relief. freedom from complications and enhanced healing which follows surgery under AA. The major disadvantages are the long induction period (10-40 minutes) and variable degrees of analgesia, even in skilled hands. The mechanisms of AA involve stimulation of peripheral sensory nerve, spinal cord and supraspinal areas (thalamus, midbrain and hypothalamus). AA can be used effectively in dog. The method has its advantages and disadvantages.

수박 종자에서 Pseudomonas pseudoalcaligenes subsp. citrulli의 분리를 위한 선택배지

金炯武,李光植 全北大學校 1993 論文集 Vol.36 No.-

A semiselctive agar medium(PSC) was developed for the isolation of Pseudomonas pseudoalcaligenes subsp. citrulli(Ps.c.) from watermelon seed. The medium contains following per liter : 20g proteose peptone #3, 5.0㎖ glycerol, 1.5g K2HPO4, 1.5g MgSO47H2O, 15g agar, 1㎎ ampicillin, 100㎎ cycloheximide 1㎖ 1.5% bromthymol blue, 1㎖ 1.0% methyl violet and 1㎎ vancomycin. Colonies of Ps.c. were 3-4㎜ in diameter, smooth, round, slight convext, dark green(lighter margin) and glistening after 5 days at 39C on the PSC medium. The recovery of 10 strains including ATTC type 29625, on PSC medium in comparison to KB ranged from 93% to 105%(mean 97.8%). Reduction in seed flora of 10 watermelon seed lots on PSC medium was 97.2%. Recovery of Ps.c. added to watermelon seed ranged from 91.3% to 103.4%(mean 96.7%).

최근 일간지에 보도된 정신병에 대한 기사 분석

김성완,윤진상,이무석,이형영 大韓神經精神醫學會 2000 신경정신의학 Vol.39 No.5

연구목적: 정신병에 대한 사회적 편견과 낙인은 질병의 예방, 조기치료 및 재활을 어렵게 한다. 이러한 부정적 시각은 다양한 대중매체에 의해서 강화될 수 있다. 본 연구는 질병에 대한 일반인의 인식에 큰 영향을 줄 수 있는 신문이 정신병에 대해 기술하는 내용을 분석하였다. 방법: 1998년 3월부터 2000년 2월까지 2년 동안 동아일보와 조선일보의 신문기사를 천리안의 뉴스복수정보 검색 프로그램을 이용하여 조사하였다. 검색어로는 일반인과 신문기사가 정신병을 지칭한다고 생각되는 정신분열, 정신병, 정신질환, 정신이상을 선택하였다. 이러한 검색어를 포함한 총 326건의 기사를 정해진 범주에 따라 분류하여 양적 및 질적 분석을 하였다. 정신병에 대한 시각을 알아보기 위해 신문기사를 부정적(직접적 또는 간접적) ② 객관적 또는 긍정적, ③특별한 시각 없음으로 분류하였다. 결과: 326건의 기사 중 부정적 기사는 직접적 177건, 간접적 51건으로 모두 228건(69.9%)이었다. 반면 객관적 또는 긍정적인 기사는 각각 29건, 14건으로 총 43건(13.2%)이었고 특별한 시각 없음은 55건(16.9%)이었다. 부정적 기사 중 가장 많은 것은 정신병 환자는 위험하거나 난폭하며 범죄를 잘 저지른다는 용을 전달하는 기사였다(118건). 이외에도 정신병 환자는 엉뚱하거나 특이하다(27건), 사회적 기능 행을 잘 못한다(18건), 정신병은 사회적으로 창피한 병이다(14건), 낫지 않는 만성질병이며 가족은 힘들고 부담스럽다(16건), 격리 수용해야 한다(6건)는 부정적 측면의 내용이 있었다. 또한 병의 원인에 대한 편견(18건), 상대를 비하하거나 어떤 현상의 심각성을 표현하기 위해 정신병이란 용어가 사용되는 경우(35건)와 더불어, 정신병원 입원에 대해서도 수용, 감금, 처넣다 등의 단어로 부정적인 기술(18건)을 하였다. 정신이상, 정신병이라는 용어를 사용하는 경우에 부정적 기사의 비율이 높았고, 기사에 환자가 직접 묘사되는 경우에 부정적 기사의 빈도가 높았다. 사건뉴스, 정치, 방송, 문화 기사에서 부정적 기사가 많았다. 반면 건강 기사에서 부정적 기사가 상대적으로 적었고 객관적 또는 긍정적 기사의 비율은 가장 높았다. 결론: 정신병에 대한 신문의 올바른 보도를 유도하는 것은 일반인의 질병개념 형성과 치료방법의 선택에 긍정적인 영향을 미칠 것이며, 나아가 환자의 효율적인 사회적응에 도움이 될 것이다. 이를 위해 정신병에 대한 신문기사의 부정적인 기술과 편견 전달을 체계적으로 모니터 하는 것이 필요하다고 생각한다. 또한 대중매체를 적극적으로 활용하여 정신병에 대한 정보를 일반인에게 사실적으로 전달하려는 노력이 요구된다. 중심단어:정신병·신문·대중매체·낙인·정신분열. Object: Social stigma and prejudice against schizophrenia hinder the prevention of the disease, early treatment and social rehabilitation. This stigma and the negative viewpoint against schizophrenia are enhanced by the mass media. This study investigated newspaper articles on psychosis, which strongly influence the public recognition of the illness. Method: The newspaper atricles of Dong-A Ilbo and Chosun Ilbo from March 1998 to February 2000 were reviewed through a news-searching program in Chollian. The search words, which were regared as psychotic illness, were Schizophrenia, Psychosis, Mental illness, and Mental derangement. A total of 326 articles were classified by category and analyzed quantitatively and qualitatively. The viewpoint of articles on psychosis consisted of these : ①negative(direct or indirect) ② neutral or positive ③ no specific viewpoint. Result: Of the 326 articles, the number with a negative description was 228(69.9%), the number with a neutral or positive description was 43(13.2%), and the number with no specific viewpoint was 55(16.9%). The most frequent negative theme was psychotic patients are dangerous or violent or may commit a crime(n=118). Other negative themes were these : psychotic patients are bizarre or grotesque(n=27), psychotic patients can't function in society well(n=18), psychosis is incurable and families should bear great burden(n=16), psychosis is shameful(n=13) and psychotic patients should be institutionalized(n=6). There were also prejudices against the cause of the illness(n=18), the description of psychosis in degrading terms(n=22) and the negative description of psychiatric hospitalization(n=18) with the words such as institutionalization, imprisonment and put into etc. In the artcles using the terms mental derangement or psychosis compared with mental illness, schizophrenia and in the articles describing psychotic patient compared with illness itself, the frequency of negative articles was high. Within each type of artcle, news, politics and culture artcles more frequently had a negative description, while health artcles less frequently had a negative description and most frequently had a neutral or positive description. Conclusion: There should be a program for monitoring newspapers for appropriate in-formation on psychosis. It might reduce social stigma and consequently help rehabilitation of psychotic patients. Also there should be endeavors to monitor other mass media and to develop a public education campaign through the mass media. KEY WORDS:Psychosis·Newspaper·Media·Stigma·schizophrenia.