Methylation Status and Expression of E-cadherin in Oral Squamous Cell Carcinomas Compared to Benign Oral Epithelial Lesions

Son, Hyun-Jin,Chu, Jung-Youb,Cho, Eui-Sic,Lee, Dong-Geun,Min, Myung-Gee,Lee, Suk-Keun,Cho, Nam-Pyo The Korean Academy of Oral Biology 2006 International Journal of Oral Biology Vol.31 No.2

Expression of invasion/metastasis suppressor, E-cadherin, is reduced in many types of human carcinomas. Although somatic and germline utations in the CDH1, which encodes the human E-cadherin, have frequently been reported in cases with diffuse gastric and lobular breast ancers, irreversible genetic inactivations are rare in other human carcinomas. Recently, it has been well documented that some genes in human cancers may be inactivated by altered CpG methylation. Herein, we determined the expression and methylation status of E-cadherin in oral squamous cell carcinoma (SCC) by immunohistochemistry and methylation-specific PCR. The expression of E-cadherin was significantly higher in the well-differentiated oral SCCs than the moderately or poorly differentiated ones. None of eight tested benign pithelial hyperplasias showed aberrant methylation, whereas five of 12 oral squamous cell carcinomas showed aberrant methylation. When we compared E-cadherin expression with methylation status, oral SCCs with normal methylation showed a higher expression of E-cadherin than those with methylation. These findings suggest that aberrant CpG methylation of CDH1 promoter region is closely associated with transcriptional inactivation and might be involved in tumor progression of the oral mucosa.

서비스의 개념과 척도에 관한 연구

조현철,강석후 漢陽大學校 産業經營硏究所 1992 産業經營硏究 Vol.4 No.-

개구리(Rana nigromaculata)胃 粘液質의 組織化學的 硏究

趙顯旭,朴重錫,河在淸 慶尙大學校 1985 論文集 Vol.24 No.2

개구리 위 점막내에 존재하는 점액질의 성분을 변태 시기에 따라서 조직화학적으로 관찰하였다. 원주세포 내 점액질은 변태 XVI와 XXIV 시기에는 바황화점액질로 된 산성점액질에 비해서 중성점액질이 주를 이루었고 변태 XX 시기에서는 산성 및 중성점액질이 혼재하였다. 점액선 세포내 점액질은 관찰된 변태 세 시기에서는 주로 중성점액질로 되어 있었다 This study was-performed histochemically to observe the responses of mucus secreting cells in the gastric mucosa of frog, Rana nigromaculata during metamorphosis. The obtained results were as follows: 1. Columnar cells contained a small amount of acidic mucosubstances including nonsulfated. mucin and a large amount of neutral mucosubstances at XVI and XXIV stages of metamorphosis. At XX stage, columnar cells contained acidic and neutral mucosubstances. 2. Mucous glands contained mainly neutral mucosubstances at XVI, XX and XXIV stages.

디푸루칸 캅셀(플루코나졸 50 mg)에 대한 플루코나 캅셀의 생물학적 동등성

조혜영,강현아,이석,오인준,임동구,문재동,이용복 한국약제학회 2003 Journal of Pharmaceutical Investigation Vol.33 No.2

Fluconazole is an orally active bis-triazole antifungal agent, which is used in the treatment of superficial and systemic candidiasis and in the treatment of cryptococcal infections in patients with the acquired immuno deficiency syndrome (AIDS). The purpose of the present study was to evaluate the bioequivalence of two fluconazole capsules, Diflucan(Pfizer Pharmaceuticals Korea Inc.) and Flucona (Korean Drug Pharmaceuticals Co., Ltd.), according to the guidelines of Korea Food and Drug Administration(KFDA). The fluconazole release from the two fluconazole capsules in vitro was tested using KP Ⅶ Apparatus Ⅱ method at 0.1M hydrochloride dissolution media. Twenty normal male volunteers, 23.60±1.88 years in age and 63.57±6.17㎏ in body weight, were divided into two groups and a randomized 2×2 cross-over study was employed. After three capsules containing 50㎎ as fluconazole was orally administered, blood was taken at predetermined time intervals and the concentrations of fluconazole in serum were determined using HPLC method with UV detector. The dissolution profiles of two fluconazole capsules were very similar at 0.1M hydrochloride dissolution media. Besides, the pharmacokinetic parameters such as AUC_t, C_max and T_max were calculated and ANOVA test was utilized for the statistical analysis of the parameters using logarithmically transformed AUC_t and C_max and untransformed T_max. The results showed that the differences in AUC_t, C_max and T_max between two capsules based on the Diflucan were 4.96%, 5.65% and -13.76%, respectively. There were no sequence effects between two capsules in these parameters. The 90% confidence intervals using logarithmically transformed data were within the acceptance range of log(0.8) to log(1.25) (e.g., log(1.01)∼log(1.08) and log(1.00)∼log(1.12) for AUC_t and C_max respectively), indicating that Flucona capsule is bioequivalent to Diflucan capsule.

엘도스 캡슐(에르도스테인 300mg)에 대한 엘브론 캡슐의 생물학적 동등성

조혜영,이석,강현아,문재동,이용복 한국약제학회 2003 Journal of Pharmaceutical Investigation Vol.33 No.3

Erdosteine, the thiol derivatives chemically related to cysteine, is a mucolytic and mucoregulator agent which modulated mucus production and viscosity and increases mucoiliary transport. The purpose of the present study was to evaluate the bioequivalence of two erdosteine capsules, Erdos (Dae Woong Pharmaceutical Co., Korea) and Erblon (Kuhn Ⅱ Pharmaceutical Co., Korea), according to the guidelines of Korea Food and Drug Administration (KFDA). The erdosteine release from the two erodisteine capsules in vitro was tested using KP Ⅷ Apparatus Ⅱ method with various different kinds of dissolution media (pH 1.2, 4.0, 6.8 buffer solution and water). Twenty four healthy male subject, 23.33±2.06 years in age and 66.18±8.19 ㎏ in body weight, were divided into two groups and a randomized 2×2 cross-over study was employed. After three capsules containing 300 ㎎ as erdostein were orally administered, blood was taken at predetermined time intervals and the concentrations of erdostein in serum were determined using HPLC method with UV detector. The dissolution profiles of two formulations were similar at all dissolution media. Besides, the pharmacokinetic parameters such as AUC_(t), C_(max) and T_(max) were calculated and ANOVA test was utilized for the statistical analysis of the parameters using log-arithmically transformed AUC_(t) and C_(max) and untransformed T_(max). The results showed that the differences between two formulations based on the Erdos were 0.20%, 1.10% and -9.44% for AUC_(t), C_(max) and T_(max), respectively. There were no sequence effects between two formulations in these parameters. The 90% confidence intervals using logarithmically transformed data were within the acceptance range of long(0.8) to log(1.25)(e.g., long(0.94)∼long(1.22) and log(0.92)∼log(1.20) for AUC_(t) and C_(max), respectively. Thus, the criteria of the KFDA guidelines for the bioequivalence was satisfied, indicating Erblon capsule and Erdos capsule are bioequivalent.

양파(Allium cepa L.) 추출물이 Tyrosinase 유전자 발현에 미치는 효과

조남철,윤연희,이혜진,손현정,김양경,최근희,나명석,조영권,이황희,진종언 한국식품영양학회 2001 韓國食品營養學會誌 Vol.14 No.3

Melanin 생성에 관여하는 tyrosinase 유전자의 발현을 억제하는 물질을 탐색하고자 tyrosinase promoter를 지닌 Bl6 mouse melanoma cell해 앙파 methanol 추출물을 처리한 바 양파 methanol 추풀물은 10.0㎍/ml, 100.0 ㎍/ml, 1.0 ㎎/ml의 농도에서 대조군에 비해서 약 15%, 23% 57%의 억제효과를 나타냈으며 세포생존율은 1.0 ㎍/ml, 10.0 ㎍/ml, I00.0 ㎍/ml, 1.0㎎/ml의 농도에서 약 126 %, 92%. 85%, 64%로서 세포독성이 낮게 나타났다. Ethyl acetate, butyl alcohol, 그리고 물 용매 분획물은 tyrosinase 유전자의 발현물 억제하는 효과가 없었지만 methylene chloride 용해 분획물은 10.0 ㎍/ml과 100.0 ㎍/ml의 농도에서 약 37%와 35%의 발현율을 나타냄으로서 대조군에 비해 크게 억제하였다. Onion(Allium cepa L,) extract by methanol repressed the expression of tyrosinase gene of B16 mouse melanoma cell containing tyrosinase promoter. 10.0 ㎍/ml, 100.0 ㎍/ml, 1.0 ㎎/ml of the extract repressed expression of tyrosinase gene about 15%, 23%, and 57%, respectively, compared with control. In the MTT assay, the same extract exhibited low cytotoxicity at 1.0 ㎍/ml, 10.0 ㎍/ml, 100.0 ㎍/ml, and 1.0 ㎎/ml, respectively. The fractions of ethyl acetate, butyl alcohol, and water did not showed the repressive effect on the expression of tyrosinase gene, but the fraction of methylene chloride repressed highly at 10.0 ㎍/ml and 100.0 ㎍/ml.

개구리에서 산-염기 평형의 계절적 변동에 관한 연구

조석현,박해근 충남대학교 의과대학 지역사회의학연구소 1984 충남의대잡지 Vol.11 No.1

The influence of environmental temperature on the acid base balance of frog(Rana Temporaria Chosenica) blood was investigated at 25℃ all the year round. pH of blood was measured by means of Artrup method and Sigaard- Anderson's nomogram using pH meter(Corning) and tonometer. Buffer value, actual bicarbonate concentration and OH^-/H^+ ratio were calculatedd by the measure buffer line, pH and CO2 gas tension which was used(low CO_2 was 4-5% and high C0_2 was 8-9% balanced with 0_2). Results obtained are as follows: 1. Buffer line was shift to the upper and-right side during winter season and the differences was statistically significant. 2. Buffer value was higher during winter season than summer value and the difference was significan (p<0.001). 3. The actual blood pH was 7.42±0.01 in September and 7.78±0.03 in January. Generally the pH was higher during winter season than that of summer. The difference between two seasons was statistically significant(p<0.05). 4. The blood PCO_2 value has a seasonal changes with low PC0_2 during winter season and the differences were statistically significant. 5. The plasma bicarbonate concentration was 17.00±1.07mM/L in September and the value were decreased with cold weather of 12.33±1.29mM/L in January. Seasonal changes were significantly different(p<0.05). It is concluded from this findings that the acid-base changes of blood in frog were related to the temperature of living habitat and the corresponding changes in the tissue energy store and buffers.

투기종목 선수들의 무산소성 역치의 특성

조현철,송순천,김춘경,김의영,안용규,이규석,김학렬 龍仁大學校 體育科學硏究所 1991 體育科學硏究論叢 Vol.1 No.1

The purpose of the present study is to determine the levels of maximal oxygen uptake and anaerobic threshold of boxing players(n=23), ken-do players(n=18), Judo players(n=12), T.D.K players(n=18). Treadmill test was used to measure maximal state and anaerobic threshold of metabolic variables. Treadmill exercises protocol was 2-min incremental test from an initial work load of 0MPH(grade : 0%) to all-out by step of 1.0MPH(grade : 2%). The results of measurement was as follows : 1. Absolute(1/min) and relative(ml/kg/min) maximal oxygen uptake was highest(5.08l/min, 65.01 ml/kg/min) in Judo layers, followed in order by the T.D.K players (4.37l/min, 64.74 ml/kg/min), Boxing players(4.18) l/min, 63.19 ml/kg/min) and ken-do players(3.81l/min, 58.65 ml/kg/min). It is not significant difference in the relative maximal oxygen uptake, but is significant difference between group in the absolute maximal oxygen uptake(p<0.001) 2. AT-VO₂(l/min) and AT-VO₂(ml/kg/min) in the anaerobic threshold was highest (3.71 l/min, 47.28 ml/kg/min) in Judo players, followed in order by the T.K.D players(3.29 l/min, 48.75 ml/kg/min), boxing players(2.90 l/min, 43.91 ml/kg/min) and ken-do players(2.82 l/min, 43.52 ml/kg/min). It is not significant difference in the AT-V02(ml/kg/min), but is significant difference between group in the AT-V02(l/min). (p<0.001) 3. %HRmax and %V 02max in the anaerobic threshold was not significant difference between group.

유방암의 위와 대장전이

유현아,김은영,서민지,정은,조민정,오현진,장지혜,박지찬,이정의,박석영 이화여자대학교 의과학연구소 2014 EMJ (Ewha medical journal) Vol.37 No.2

Gastric metastasis from breast cancer is rare and only six cases have been reported in Korea. Colon metastasis is more rare than gastric metastasis. We report a 63-year-old woman with gastric and colon metastases of invasive lobular carcinoma of breast. She was diagnosed as right breast cancer, received right modified radical mastectomy 10 years ago and has been treated with chemotherapy and hormone therapy. Investigating for melena and a small caliber of stool, we found gastric and colon metastases. The diagnosis of metastatic breast cancer was made through gross pathologic and immunohistochemistry staining. We report a case with gastric and colon metastases from breast cancer and a review of the associated six case reports in Korea.

리스페달 정(리스페리돈 2㎎)에 대한 리스펜 정의 생물학적 동등성

조혜영,박은자,강현아,백승희,이석,박찬호,문재동,이용복 한국약제학회 2004 Journal of Pharmaceutical Investigation Vol.34 No.2

The purpose of the present study was to evaluate the bioequivalence of two risperidone tablets, Risperdal (Janssen Korea Co., Ltd) and Rispen (Myung In Pharm. Co., Ltd), according to the guidelines of Korea Food and Drug Administration (KFDA). The risperione release from the two risperidone formulations in vitro was tested using KP Ⅷ Apparatus Ⅱ method with various of dissolution media (pH 1.2, 4.0, 6.8 butter solution and water). Twenty four healthy male subjects, 23.33±2.10 years in age and 69.24±8.05 kg in body weight, were divided into two groups and a randomized 2×2 crossover study was employed. After one tablet containing 2 ㎎ as risperidone was orally administered, blood was taken at predetermined time intervals and the concentration of risperidone in serum were determined using HPLC method with UV detector. The dissolution profiles of two formulations were similar at all dissolution media. Besides, the pharmacokinetic parameters such as AUC_(t), C_(max) were calculated and ANOVA test was utilized for the statistical analysis of the parameters using logarithmically transformed AUC_(t), C_(max) and untransformed T_(max). The results showed that the differences between two formulations based on the Risperdal were 0.20, -1.29 and -11.09% for AUC_(t), C_(max) and T_(max), respectively. There were no sequence effects between two formulations in these parameters. The 90% confidence intervals using logarithmically transformed data were within the acceptance range of log(0.8) to log(1.25) (e.g., log(0.90)∼log(1.03) and log(0.84)∼log(1.09) for AUC_(t) and C_(max), respectively). Thus, the criteria of the KFDA guideline for the bioequivalence were satisfied, indicating Rispen tablet and Risperdal tablet were bioequivalent.