Slide Session : OS-IFD-07 ; Infectious Disease : In Vitro Antiviral Activity of Ribavirin Against Severe Fever with Thrombocytopenia Syndrome Virus

( Myung Jin Lee ),( Kye Hyung Kim ),( Jong Youn Yi ),( Su Jin Choi ),( Chung Jong Kim ),( Nak Hyun Kim ),( Kyoung Ho Song ),( Pyoeng Gyun Choi ),( Ji Hwan Bang ),( Wan Beom Park ),( Eu Suk Kim ),( San 대한내과학회 2014 대한내과학회 추계학술대회 Vol.2014 No.1

In Vitro Antiviral Activity of Ribavirin Against Severe Fever with Thrombocytopenia Syndrome Virus Myung Jin LEE1, Kye-Hyung KIM1, Jongyoun YI2, SuJin CHOI1, Chung-Jong KIM1, Nak- Hyun KIM1, Kyoung-Ho SONG1, Pyoeng Gyun CHOI1, Ji-Hwan BANG1, Wan Beom PARK1, Eu Suk KIM1, Sang-Won PARK1, Hong Bin KIM1, Nam Joong KIM1, Myoung- Don OH1 Seoul National University College of Medicine, Korea1, Pusan National University School of Medicine, Korea2 Background: Severe fever with thrombocytopenia syndrome (SFTS) is an emerging infectious disease caused by a novel Bunyavirus, severe fever with thrombocytopenia syndrome virus (SFTSV). No effective antiviral therapy is proven yet, but clinical use of ribavirin (RBV) has been tried. We investigated the antiviral effect of RBV against SFTSV in vitro. Methods: To test for cytotoxicity of RBV, Vero cells were treated with different concentrations of RBV (3.90 to 500 μg/mL, two-fold dilution) and analyzed by cell viability MTS assay 48h post-infection. To determine antiviral activity of RBV against SFTSV, Vero cells were infected with SFTSV strain Gangwon/Korea/2012 at 100 TCID50 (50% tissue culture infective dose) per well in a 96-well plate, and RBV was added at the concentrations showing no or minimal cytotoxicity. Viral RNAs were extracted from the culture supernatants and quantifi ed using one-step real-time reverse transcription- PCR to amplify the partial large segment of SFTSV. Statistical analysis was done by one-way ANOVA with Tukey`s post hoc test. Results: Cytotoxicity due to RBV was not observed at RBV concentration =31.3 μg/ mL. Viral RNAs at 24h post-RBV treatment were reduced with increasing RBV concentrations (1-32 μg/mL), compared with those of mock-treated cells (P <0.01, Figure). Half maximal inhibitory concentration (IC50) of RBV was 3.69 μg/mL at 24h post-RBV treatment. Conclusions: Our study shows that RBV has antiviral effect against SFTSV in a dose-dependent manner. Further studies are required to evaluate the effi cacy of RBV in SFTS.

트레드밀 운동시 흡기근 테이핑이 폐활량에 미치는 영향

김민지,신수영,송월섭,조수진,최동락,황미진,황진규,박진현,김경,Dennis W. Fell 대구대학교 특수교육재활과학연구소 2011 再活科學硏究 Vol.29 No.1

이 연구는 트레드밀 운동과 키네시오 테이핑의 효과에 따른 폐활량의 변화를 알아보기 위해 26명의 비흡연자가 참가하였으며 키네시오 테이핑을 적용하지 않은 그룹과 키네시오 테이핑을 적용한 두 그룹으로 무작위로 배정하고 각 군들을 주 3회 6주간의 트레드밀 훈련을 실시하였다. 실험 전과 후, 스파이로미터를 사용하여 키네시오 테이핑의 적용 따른 폐활량 변화 효과를 측정하였다. 이 연구의 결과를 종합해보면 키네시오 테이핑을 적용 그룹에서 적용하지 않은 그룹에 비해 FVC, FEV1에서 유의한 증가를 보였다. 이러한 결과는 건강한 성인에서 키네시오 테이핑을 적용한 경우 키네시오 테이핑을 적용하지 않고 트레드밀 운동을 한 경우보다 폐활량의 향상에 효과적이라고 생각되어진다. The purpose of this study was to investigate the variation of vital capacity(VC) according to the effects of kinesio taping with treadmill exercise. Twenty-six non-smokers were participated in this research and these subjects were randomly assigned into two groups. To measure the VC variation effects of kinesio taping, spirometer was used. The collected data were analyzed statistically by using a paired Mauchly test and repeated measure ANOVA. The results of this study were as follows; A group: treadmill with kinesio taping B group: treadmill without kinesio taping. Between A and B, there were significant differences. In the case of A group, there was 11.66% increase of VC, during 3 weeks experiment(p<.01). In the case of B group, there were significant differences, 3.35% increase of VC, during 3 weeks taping intervention(p<.01). After 6 weeks experiment, the improvement of VC shown a significant difference with intergroup (p<.05). From this result, it was revealed that treadmill exercise with kinesio taping was effective to improve VC to healthy adult than treadmill exercise without kinesio taping.

Helicobacter pylori의 생육에 영향을 미치는 성분과 식품첨가물에 관한 연구

송재철,정혜진,박현정,조은경,신완철 한국식품영양학회 1999 韓國食品營養學會誌 Vol.12 No.5

본 연구에서는 일반적으로 섭취하고 있는 식품재료나 음식 중에 공통적으로 함유한 성분 또는 가공중에 많이 첨가되는 성분을 선정하여 이들이 Helicobacter pylori 생육에 어떤 영향을 미치는지를 검토하였다. 첫째, 일반식품의 주요 성분인 탄소원, 질소원, 무기질을 대상으로 Helicobacter pylori 생육에 미치는 영향을 검토하였다. 그 결과 식품성분 중 탄소원의 종류에 따라 균일 생육활성은 다르게 나타났다. 우선 단당류가 이당류나 다당류에 비교하여 생육을 촉진하였고 특히 단당류 중 포도당은 가장 균의 생육에 큰 영향을 미치는 것으로 나타났다. 그러나 만노오스는 균의 생육을 오히려 억제시키는 것으로 나타났다. 당알코올류인 솔비톨, 만니톨, 말티톨, 크리시톨 등은 균의 생육에 아무런 영향을 미치지 않은 것으로 나타났다. 질소원과 Helicobacter pylori의 생육과의 관계는 평소 식생활에서 많이 섭취된다고 생각되는 일반 음식에 많이 함유되어 있는 유기형 아미노산 성분고 무기형 질소화합물을 대상으로 균의 생육 정도를 비교하였다. 리신, 메티오닌, 시스테인, 트립토판 등은 균의 생육을 촉진하고 아르기닌, 황산암모늄, 염화암모늄 등은 균의 생육에 도움이 되지 않고 암노니아, 요소등은 균의 생육에 도움이 되지 않고 암모니아, 요소 등은 균의 생육에 조금 영향을 미침을 관찰할 수 있었다. 특히 리신과 메티오닌은 가장 균의 생육에 영향을 미치는 것으로 나타났다. 무기염류의 영향에서는 황산마그네슘, KH-2PO_4, 염화마그네슘 등은 큰 영향을 미치지 못하였고 황산철, 염화제일철, 염화제이철은 생육을 억제하는 것으로 나타났다. 식품첨가물의 Helicobacter pylori의 생육 정도를 검토한 것으로, 그 다음이 질화나트륨, 염화나트륨 순이었으며 토코페롤은 전혀 생육에 영향을 미치지 못하였다. The purpose of this study was to choose principal food components contained in diet foods and food additives used for manufacturing processed foods, and elucidate their in vivo effects on the growth pattern of Helicobacter pylori. To do this, the antibacterial effects of various sources of carbon, nitrogen and mineral as an effect agent on Helicobacter pylori were first assessed based upon bacterial growth degree. Results show that the source of carbon tested had different effects on bacterial growth of Helicobacter pylori. It was revealed that a promotional effect of monosaccharides resulted in enhanced growth of Helicobacter pylori compared with disaccharides and polysaccharides. In particular, glucose was observed to be most effective in growth of Helicobacter pylori among monosaccharides tested, whereas mannose to hinder the growth of Helicobacter pylori. Polyols such as sorbitol, mannitol, maltitol and xylitol was, however, observed to show no promotion or suppression effect on growth of Helicobacter pylori. Apart from these, the sources of amino acid and inorganic nitrogen were chosen and tested to assess the promotion or suppression effect of nitrogen sources on growth of Helicobacter pylori. It was found that amino acid such as lysine showed its promotion effect on the growth of Helicobacter pylori, while arginine, (NH_1)_2SO_4 and NH_4Cl showed no effect on its growth. Ammonia and urea were, however, observed to have a positive effect on the growth of Helicobacter pylori. Among these effect agents, lysine and methionine were revealed to show the most positive effect on growth of Helicobacter pylori. Minerals such as MgSO_4, KH_2PO_4, and MgCl_2 appeared to exert their positive growth effects, whereas CaCl_2, and CaSO_4 had a little effect. In addition FeSO_4, FeCl_2, and FeCl_3 brought suppression on the growth of Helicobacter Pylori. In studies of the growth of Helicobacter pylori by food additives, ascorbic acid showed extreme suppression on its growth. Sodium nitrate and sodium chloride were also found to be of negative effect on the growth of Helicobacter pylori in order of degree, whereas tocopherol had nothing to do with microbial growth.

백양꽃(Lycoris koreana) × 개상사화(Lycoris aurea) 교잡 미숙배로부터 식물체 생산

송원섭,박인현,박진형 圓光大學校大學院 1992 論文集 Vol.10 No.-

This experiment was conducted to investigate cultural conditions of immature embryo in Lycoris koreana×Lycoris aurea hybrid. Optimum concentration of ager supplemented to Murashige and Tucker(MT) medium for plantlet production was 6g/l. Optimum concentration of sucrose supplemented to MT medium for plantlet production was 30~50 g/l. For plantlet prodution, MT medium supplemented with NAA 0.01 mg/l plus zeatin 0.1~0.01mg/l and without olant growth regulator was most effective. Optimum level of PH controlled to MT medium for plantlet productiom was 4.5~5.0. Key words : Lycoris koreana, Lycoris aurea, immature embryo, plantlet production.

개인식별을 위한 컴퓨터 영상 중첩시스템 연구

송현교,김하진,강민구 호남대학교 정보통신연구소 1996 정보통신연구 Vol.5 No.-

임포즈란 신원 미상의 두개골을 발견했을 때, 두개골의 사진과 동일인으로 판정되는 사람의 생전 사진을 서로 같은 비율로 축소한 후 두 사진을 겹침으로써 두 인물간의 동일인 여부를 비교, 검토하는 과정을 말한다. 이에 본 논문에서는 국립과학수사연구소에서 제 검사 및 DNA 유전자 검사를 통해 이미 동일인임이 확인된 7명의 두개골 사진, 생전사진을 이용하여 컴퓨터 프로그램을 이용한 응용프로그램을 개발하였다. 신원 미상의 두개골을 비디오 카메라, 컴퓨터를 이용, 영상처리후 임포즈하고 해부학적 특징으 기준으로 동일인여부를 비교, 검토하는 과정에서 다음과 같은 결과를 얻었다. 1. 두개골 및 생전사진을 처리하는 방법으로 다양한 영상처리 기법(Smooth, Sharpen, Emboss, Mosaic, Engrave, Invert, Neon, Color to Mono)을 개발하였고, 이들 중 Neon, Invert, Engrave기법에서 최적의 영상을 얻을수 있었다. 2. 다양한 방식(SrcOr. SrcAnd. SrcInvert. SrcErase,DstInvert. MergePaint) 으로 두 영상을 합성하여 임포즈 감정시 필요한 최적의 비교상태를 얻을수 있었다. 3. 비디오 카메라를 이용하여 두개골상을 직접 컴퓨터에 입력할 수 있었으며, 이를 생전사진과 임포즈함으로써 보다 정확하고 신속한 감정을 가능 하게 했다. 이상에서 볼 때 기존의 수작업으로 이루어졌던 임포즈가 이제는 컴퓨터 프로그램에 의해 이루어지므로서 보다 신속하고 정확한 임포즈 작업을 가능하게 할 것이라 사료된다. A Study on the Computer Impose System for Individual Recognization *Dept. of inform. and Comm Eng.. Honam University ** The Dental School of Yonsei University In this thesis. a new superimposition scheme using a computer vision system was proposed with 7 pairs of skull and ante-mortem photographs. which were already identified through other tests and DNA fingerprints at the Korea National Institute of scientific Investigation, At this computer vision system. an unidentified skull was caughty by video-camcoder with the MPEG and a ante-mortem photograhp was scanned by scanner. These two images were processed and superimposed using pixel processing. Recognition of the individual identification by anatomical references was performed on the two superimposed images. This image processing techniques for the superimposition of skull and ante-mortem photographs simplify used the previous approach taking skull photographs and developing it to the same size as the ante-mortem photographs. This system using various image processing techniques on computer screen. a more precise and time-saving superimposition technique could be able to be applied in the area of computer individual indentification.

유구치 치수절단시 치관부 치수의 임상, 혈액 및 병리 조직학적 관찰

송민호,김영진,김현정 大韓小兒齒科學會 1993 大韓小兒齒科學會誌 Vol.20 No.1

현제, 소아치과의 임상에서 유치치수 절단술시 치수상태를 객관적으로 파악할 수 있는 진단방법이 없으나 적절한 적응증을 선택하기 위해서는 반드시 필요하다. 따라서 저자는 생활치수 절단처치가 필요하다고 단단되는 유구치 52례를 동통의 병력,구내표준방사선사진,전기치수검사,온,냉자극검사,치수노출유무,치수내 혈액상 및 치관부 치수제거 후 지혈곤란도를 병리조직학적 소견과 비교하여 유치치수염의 감별진단의 지표로 삼고자 실험하여 아래와 같은 결과를 얻었다. 1. 유규치 치관부 치수의 병리조직상 염증정도를 단계로 분류하여(-)14례(26.9%),(+)26례(50.0%),(++)5례(9.6%)이고,(+++)7례로 나타났다. 2. 동통의 병력은 치관부 치수 염증정도별에 따라(-)35.7%,(+)30.8% (++)80.0%,(+++)85.7%로 염증이 심할수록 동통이 발생하는 비율이 높게 나타났다(p<0.05). 3. 구내표준방사선 사진상에 의한 우식부 하방의 잔존상아질 양과 염증정도 사이에는 유의한 차이가 없었다(p<0.05). 4. 연화상아질 제거 후의 치수노출은 염증정도에 따라 (-)7.1%,(+)26.9%,(++)80.0%,(-+++)71.4%로 염증이 심할수록 치수노출되는 비율이 높게 나타났다(p<0.05). 5. 전기치수검사와 온, 냉자극검사는 염증정도에 검사치 사이에 유의성이 없었다(p>0.05). 6. 치수혈액상 림프구 백분율의 평균은 염증정도에 따라(-)53.6%,(+)50.8%,(++)48.2%,(+++)37.4%로 염증이 심할수록 림프구 백분율이 감소하는 것으로 나타났다(p<0.05). 따라 (-)7.1%,(+)11.5%,(++)0%,(+++)71.4%로 염증이 심할수록 높게 나타났다(p<0.05). The purpose of the present investigation was to study the diagnosis of deciduous tooth pulpitis by the clinical examination,blood picture and histopathologic features in the coronal pulp. It makes sure of the extent of the inflammation for more appropriate pulp therapy. The subjects were 52 decayed deciduous teeth of 26 normal healthy children aged from sy5m to 10y8m. which justified the vital pulp amputation therapy. The pain history,periapical radiograph,EPT,thermal test,pulp exposure,hemogram and bleeding control were compared with the histopathologic structure of the coronal pulp. The results were as follows: As to the histologic structure of the removed moronal pulp, the inflammatory degree was divided into 4 types; (-)no evedence of inflammation,(+)slightly confined infiltration on inflammatory cells,(++)slightly or moderately diffused infiltration,(++)severely diffused infilration,when the numbers of the case respectively at degree type were(-)14,(+)26,(++)5,(+++)7 cases. The ratio of cases which have pain history is significantly higher according to the severity of inflammation(p<0.05). The ratio of cases in which the pulp is exposed is significantly higher according to the severity of inflammation(p<0.05). As the results of the X-ray examination, EPT and thermal test show, they had no correlation with the inflammatory state(p>0.05). The mean of the lymphocyte ratio was significantly decreased according to the severity of inflammation(p<0.05). The ratio of cases in which it was difficult to control bleeding at the coronal pulp amputations were significantly higher according to severity of inflammation(p<0.05).

패류의 영동에 관한 연구 : 4. 오분자기의 동결저장온도에 따른 품질의 변화

송대진,하진환,김수현 제주대학교 해양연구소 1985 해양자원연구소연구보고 Vol.9 No.-

貝類의 冷凍에 關한 硏究 : 4. 오분자기의 凍結貯藏溫度에 따른 品質의 變化 4. Quality Changes of Abalone, Haliotis diversicolor japonica, by the Freezing Storage Temperatare

宋大鎭,河璡桓,金洙賢 濟州大學校 海洋資源硏究所 1985 해양과환경연구소 연구논문집 Vol.9 No.-

오분자기를 보다 效果的으로 利用하기 위한 基礎資料를 얻기 위하여 오분자기를 脫殼, 凍結한 후 凍結貯藏中의 品質의 變化를 實驗하였다. 凍結貯藏中 pH는 거의 變化가 없었고 drip量 조금씩 增加하였으며 揮發性鹽基窒素도 조금씩 增加하였으나 -35℃의 경우 3個月까지는 좋은 鮮度를 維持하였다. 保水力은 점차 減少하여 貯藏 3個月후에는 -10℃에서 貯藏한 경우가 10%, -35℃는 약 7%까지 減少하였다. hardness는 -10℃와 -35℃ 다 같이 1個月까지 增加하다가 以後 급격하게 減少하였으며 toughness는 계속 減少하였다. cohesiveness는 -10℃의 경우 큰 變化가 없었으나 -35℃의 경우는 2個月에 급격히 減少하였으며 elasticity는 -10℃와 -35℃의 경우 모두 큰 變化가 없었다. 色調의 變化에서 a값은 큰 變化가 없었으나 L값은 계속 減少하였고 b값과 ΔE값은 계속 增加하였다. 組織學的 觀察의 結果 貯藏溫度가 높은 것일수록 氷結晶의 成長과 근속끼리의 응집이 현저함이 確認되어졌다. To obtain the basic data for utilizing abalone, Haliotis diversicolor japonica more effectively the quality changes as pH, drip, water holding capacity (WHC), texture, color difference and histological observation in shelled and frozen products during storage were carried out. It was noticed that the pH of frozen products (-10℃ and -35℃) were not changed markedly, thaw exudate (drip) and volatile basic nitrogen (VBN) were slightly increased as the storing period prolonged but fairly good freshness was maintained until 3 months in the storage of -35℃. The water holding capacity for all frozen products were steadily decreased to show 10% and 7% down at -10℃ and -35℃ respectively with 3 months storage on the base of raw materials. With all frozen products it was observed that hardness was increased up until the 1 month storage period and then suddenly dropped while toughness was decreased steadily. NO significant changes in cohesiveness with the -10℃ storage were observed but with -35℃, it was dropped rapidly after 2 months storage while elasticity was not changed markedly for all frozen products. The color difference expressed as a value was not changed markedly while the L value was decreased steadily. The b and ΔE value were increased continually as the frozen period was extended. It was confirmed that both the growth of ice crystal and aggregates of muscle bundle were prominent at the higher storage temperature in histological observation.

흉부 둔상 후 8년간 지속된 삼첨판폐쇄부전의 치료 1예

송현주,남승현,최영주,박성훈,박시훈,한재진 대한순환기학회 2004 Korean Circulation Journal Vol.34 No.4

스케일이 있는 급수관내의 유도초음파의 전파 특성에 관한 연구

송성진,김영환,이동훈,이현동,배철호,박정훈 한국비파괴검사학회 2003 한국비파괴검사학회지 Vol.23 No.1

배관 내의 스케일은 통수비를 감소시키므로 배관을 적절히 관리하기 위해서는 스케일량에 대한 정량적인 평가가 필수적이다. 상수도 급수관내의 스케일량을 평가하기 위해서 유도초음파를 사용하였다. 가변각 웻지를 사용하여 유도 초음파의 여러 모드를 발생시키고, 노후 배관을 따라서 전파하는 특성을 분석하였다. 실험적으로 관내의 스케일이 증가함에 따라서 f×d=1.5㎒·㎜인 경우에 F(M,2) 모드들의 진폭이 크게 감소하는 것을 알 수 있었다. 본 연구의 결과로 상수도 급수관의 스케일량 평가에 적합한 유도 초음파는 F(M,2) 모드들임을 알 수 있었다. Since the scale in pipes reduces the flow rate, a quantitative evaluation of the scale is essential for the proper maintenance of pipes. Guided waves were employed to estimate the amount of scale in water supplying pipes. Using variable angle wedge, several modes of guided waves were generated and their propagation charcteristics along the pipes with scale were analyzed. It was experimentally observed that the amplitude of F(M,2) modes at f×d=1.5㎒·㎜ decreased significantly with increasing amount of scale. The present study showed that F(M,2) modes were optimal to evaluate the scale in water supplying pipes.